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Showing: 3901 - 3920 of 66710 results

  2. pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.A184S

    Plasmid
    #106183
    Purpose
    Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Publication
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Has service
    AAV1
    Insert
    SF-Venus-iGluSnFR.A184S
    Use
    AAV
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184S
    Promoter
    hSynapsin
    Available since
    July 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    106183-plasmid-map-sequence-id-214796

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  4. pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP

    Plasmid
    #137141
    Purpose
    Intersectional viral expression of ChR2(ET/TC)-EYFP in cells expressing Flp AND NOT Cre
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Publication
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Has service
    AAV8
    Insert
    Coff/Fon-ChR2(ET/TC)-EYFP
    Use
    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt
    Mutation
    E123T, T159C
    Promoter
    nEF
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    137141-plasmid-map-sequence-id-301584

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  5. Spike Display_B.1.1.7

    Plasmid
    #172735
    Purpose
    Expression vector for SARS-CoV-2 (B.1.1.7) spike used in the Spike Display system
    Depositor
    IIya Finkelstein
    Publication
    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 S B.1.1.7 (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    CMV
    Available since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    172735-plasmid-map-sequence-id-340032

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  9. pSW031

    Plasmid
    #231144
    Purpose
    Used to express cyclic sortase cW11 (recognizes APXTG motif) from E. coli.
    Depositor
    Philip Cole
    Publication
    Whedon et al J Am Chem Soc. 2024 Nov 25. doi: 10.1021
    Insert
    S. aureus Sortase A mutant
    Tags
    Nostoc punctiform split intein C-terminal fragmen…
    Expression
    Bacterial
    Mutation
    P94S, E108Q, S116V, D160N, T164Q, V168M, L169H, D…
    Promoter
    T7
    Available since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    231144-plasmid-map-sequence-id-460063

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  11. pMC_mClover3_BSDminus_F2

    Plasmid
    #184167
    Purpose
    Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.
    Depositor
    Ophir Shalem
    Publication
    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    mClover3
    bsd
    Use
    CRISPR
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    184167-plasmid-map-sequence-id-360739

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  12. pMC_mClover3_BSDminus_F1

    Plasmid
    #184166
    Purpose
    Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.
    Depositor
    Ophir Shalem
    Publication
    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    mClover3
    bsd
    Use
    CRISPR
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    184166-plasmid-map-sequence-id-360737

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  17. pSG297-(Sp-gRNA)-(Sa-gRNA)-(PuroR_T2A_BFP(C>T screening))

    Plasmid
    #239448
    Purpose
    Orthogonal dual Cas9 gRNA and BFP reporter lentivirus cassette plasmid. For use with S. pyogenes and S. aureus gRNAs and includes a BFP reporter which reports on C-to-T editing.
    Depositor
    Alexis Komor
    Inserts
    S.p. gRNA backbone
    S.a. gRNA backbone
    PuroR-T2A-BFP(C>T_screening)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    BFP: T65S, S72S, V145F, K206K, H231L
    Promoter
    EF1alpha, U6, and mU6
    Available since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239448-plasmid-map-sequence-id-476590

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Showing: 3901 - 3920 of 66710 results