We narrowed to 48,240 results for: REP

1. gRNA_tdtomato_reporter1_SP-2x

   Plasmid

   #79370

   Purpose

   Assays activity of transcriptional activators fused to SP Cas9

   Depositor

   George Church

   Article

   Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

   Insert

   tdtomato
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pREPK219H

   Plasmid

   #51492

   Purpose

   pREP with a mutation in the ATPase domain (K219H). This is predicted to result in loss of geminivirus replication.

   Depositor

   Daniel Voytas

   Article

   Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

   Insert

   Rep/RepA
   

   Use

   Plant expression t-dna

   Tags

   Expression

   Mutation

   changed lysine 219 to histidine

   Promoter

   2x35S

   Available since

   May 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. 28 pCOLI_S_STREP

   Plasmid

   #160262

   Purpose

   N-term 2xStrepII 3C cleavable Spec resistant bacterial expression vector

   Depositor

   John Weir

   Article

   Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

   Type

   Empty backbone

   Use

   Tags

   2xStrepII

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pYZ_3WJrep_N20_switch

   Plasmid

   #132722

   Purpose

   T7 RNAP-driven expression of 3WJ repressor index 20 switch RNA with a GFP-ASV reporter

   Depositor

   Peng Yin, Alexander Green

   Article

   Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

   Insert

   3WJ_N20_switch
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pYZ_3WJrep_N20_trigger

   Plasmid

   #132726

   Purpose

   T7 RNAP-driven expression of 3WJ repressor index 20 trigger RNA

   Depositor

   Peng Yin, Alexander Green

   Article

   Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

   Insert

   3WJ_N20_trigger
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pYZ_3WJrep_N24_switch

   Plasmid

   #132725

   Purpose

   T7 RNAP-driven expression of 3WJ repressor index 24 switch RNA with a GFP-ASV reporter

   Depositor

   Peng Yin, Alexander Green

   Article

   Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

   Insert

   3WJ_N24_switch
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AAVS1_Puro_PGK1_3xFLAG_Twin_Strep_EZH2

   Plasmid

   #79902

   Purpose

   Gene Addition of 3xFLAG_Twin_Strep_EZH2 to the AAVS1 Genomic Safe Harbor Locus

   Depositor

   Yannick Doyon

   Article

   Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

   Insert

   3xFLAG_Twin_Strep_EZH2
   

   Use

   CRISPR and TALEN

   Tags

   3xFLAG_Twin_Strep

   Expression

   Mammalian

   Mutation

   Promoter

   PGK1

   Available since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJK_ToeRepG2_N02_switch

   Plasmid

   #132742

   Purpose

   T7 RNAP-driven expression of second-generation toehold repressor index 2 switch RNA with a GFP-ASV reporter

   Depositor

   Peng Yin, Alexander Green

   Article

   Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

   Insert

   ToeRepG2_N02_switch
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCRISPReporter

   Plasmid

   #65007

   Purpose

   E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with reporter cloning site

   Depositor

   Mattheos Koffas

   Article

   Cress et al ACS Synth Biol. 2015 Mar 30.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   none

   Available since

   May 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Bxb1Donor_WT_ADRB2_LucReporter

    Plasmid

    #129788

    Purpose

    encodes a donor vector for recombination with the Bxb1 recombinase with the WT Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

    Depositor

    Sriram Kosuri

    Article

    Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

    Insert

    ADRB2 (ADRB2 Human)
    

    Use

    Synthetic Biology

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    N/A

    Available since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pReporter_5

    Plasmid

    #60566

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJK_ToeRepG2_N19_trigger

    Plasmid

    #132747

    Purpose

    T7 RNAP-driven expression of second-generation toehold repressor index 19 trigger RNA

    Depositor

    Peng Yin, Alexander Green

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    ToeRepG2_N19_trigger
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pStrepIHFb

    Plasmid

    #149385

    Purpose

    Expression of P. aeruginosa PA14, StrepII-HRV3C-IHFbeta

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 StrepII-tagged IHFbeta
    

    Use

    Tags

    HRV3C cleavage site and StrepII-tag with

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 52 pLIB_3C_C_STREP

    Plasmid

    #160300

    Purpose

    C-term 3C cleavable 2xStrepII tag for insect cell expression

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    2xStrepII

    Expression

    Insect

    Mutation

    Promoter

    polyhydrin

    Available since

    Nov. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Preprotachykinin A

    Plasmid

    #130664

    Purpose

    Expresses human Preprotachykinin A in mammalian cells

    Depositor

    Michael Kressel

    Insert

    Preprotachykinin A (TAC1 Human)
    

    Use

    Tags

    Fusion Red

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYZ_3WJrep_N19_switch

    Plasmid

    #132723

    Purpose

    T7 RNAP-driven expression of 3WJ repressor index 19 switch RNA with a GFP-ASV reporter

    Depositor

    Peng Yin, Alexander Green

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    3WJ_N19_switch
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pReporter_2

    Plasmid

    #60563

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EmptyTranscriptionalReporter

    Plasmid

    #62453

    Purpose

    MXS_chaining vector with 2 cassettes

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Inserts

    CMVmin::NLS-Citrine-bGHpA
    CMV::NLS-Cherry-bGHpA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pReporter_7

    Plasmid

    #60567

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJK_ToeRepG2_N02_trigger

    Plasmid

    #132745

    Purpose

    T7 RNAP-driven expression of second-generation toehold repressor index 2 trigger RNA

    Depositor

    Peng Yin, Alexander Green

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    ToeRepG2_N02_trigger
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge