263,825 results

1. AAV-Best1-sCD200

   Plasmid

   #164424

   Purpose

   AAV plasmid expressing soluble CD200 in retinal pigment epithelium

   Depositor

   Connie Cepko

   Article

   Wang et al Proc Natl Acad Sci U S A. 2019 May 14;11

   Insert

   CD200 (Cd200 Mouse)
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   deleted terminal 40 amino acids

   Promoter

   Best1

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PMP34-mApple

   Plasmid

   #214417

   Purpose

   Peroxisome localization of PMP34 fused to mApple

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   PMP34 (SLC25A17 Human)
   

   Tags

   mApple

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. MAC_C_PTH2

   Plasmid

   #111674

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   PTH2 (PTRH2 Human)
   

   Expression

   Mammalian

   Available Since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. MAC_C_PTH2

   Plasmid

   #111674

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   PTH2 (PTRH2 Human)
   

   Expression

   Mammalian

   Available Since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV CAGG eGFP

   Plasmid

   #107707

   Purpose

   express eGFP in variety of cell types

   Depositor

   Troy Margrie

   Article

   Velez-Fort et al Neuron. 2018 Apr 4;98(1):179-191.e6. doi

   Insert

   eGFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAGG

   Available Since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV CAGG eGFP

   Plasmid

   #107707

   Purpose

   express eGFP in variety of cell types

   Depositor

   Troy Margrie

   Article

   Velez-Fort et al Neuron. 2018 Apr 4;98(1):179-191.e6. doi

   Insert

   eGFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAGG

   Available Since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBIG2abc

   Plasmid

   #80617

   Purpose

   pBIG2abc - Step2 vector abc (biGBac system for expression of protein complexes in insect cells)

   Depositor

   Jan-Michael Peters

   Article

   Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBIG2abc

   Plasmid

   #80617

   Purpose

   pBIG2abc - Step2 vector abc (biGBac system for expression of protein complexes in insect cells)

   Depositor

   Jan-Michael Peters

   Article

   Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCIneoMyc UNC119B

   Plasmid

   #128333

   Purpose

   Mammalian expression vector of Myc-tagged human UNC119B

   Depositor

   Yutaka Hata

   Article

   Iwasa et al Cancer Sci. 2018 Sep;109(9):2767-2780. d

   Insert

   UNC119B (UNC119B Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCIneoMyc UNC119B

    Plasmid

    #128333

    Purpose

    Mammalian expression vector of Myc-tagged human UNC119B

    Depositor

    Yutaka Hata

    Article

    Iwasa et al Cancer Sci. 2018 Sep;109(9):2767-2780. d

    Insert

    UNC119B (UNC119B Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Flag-mFoxJ1

    Plasmid

    #122965

    Purpose

    Expresses Flag-tagged mouse FoxJ1 in mammalian cells

    Depositor

    FENG-QIAN Li, Ken-Ichi Takemaru

    Insert

    mouse FoxJ1 (Foxj1 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Flag-mFoxJ1

    Plasmid

    #122965

    Purpose

    Expresses Flag-tagged mouse FoxJ1 in mammalian cells

    Depositor

    FENG-QIAN Li, Ken-Ichi Takemaru

    Insert

    mouse FoxJ1 (Foxj1 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBIG2abcde

    Plasmid

    #80619

    Purpose

    pBIG2abcde - Step2 vector abcde (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBIG2abcde

    Plasmid

    #80619

    Purpose

    pBIG2abcde - Step2 vector abcde (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pScRad52-SpCas9 (pXK-1646)

    Plasmid

    #208823

    Purpose

    CRISPR/Rad52-Cas9 expression vector for Animal gene editing, harboring the ScRad52-SpCas9 and a VEGFA.gRNA expression cassette. Key Construct: hU6-VEGFa.gRNA-CMV-ScRad52-SpCas9.

    Depositor

    Kun Xu

    Article

    Shao et al Int J Biochem Cell Biol. 2017 Nov;92:43-

    Type

    Empty backbone

    Use

    CRISPR; Cas9

    Tags

    Rad52

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBIG2abcd

    Plasmid

    #80618

    Purpose

    pBIG2abcd - Step2 vector abcd (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pBIG2abcd

    Plasmid

    #80618

    Purpose

    pBIG2abcd - Step2 vector abcd (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

18. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-Phi29 (JO582)

    Plasmid

    #208954

    Purpose

    A variant CE1 construct with Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-Phi29(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A), Phi29(-exo;D169A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBIG1c

    Plasmid

    #80613

    Purpose

    pBIG1c - Step1 vector c (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBIG1c

    Plasmid

    #80613

    Purpose

    pBIG1c - Step1 vector c (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAF0443 Nm60_attP-mCherry donor

    Plasmid

    #193471

    Purpose

    Promoterless mcherry donor for Nm60

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Nm60_attP
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBIG1d

    Plasmid

    #80614

    Purpose

    pBIG1d - Step1 vector d (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBIG1d

    Plasmid

    #80614

    Purpose

    pBIG1d - Step1 vector d (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBIG1e

    Plasmid

    #80615

    Purpose

    pBIG1e - Step1 vector e (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBIG1e

    Plasmid

    #80615

    Purpose

    pBIG1e - Step1 vector e (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TOP-H2B-mCherry-DD

    Plasmid

    #96894

    Purpose

    Reporter to monitor translation of 5'TOP mRNA

    Depositor

    Tobias Meyer

    Article

    Han et al Nat Methods. 2014 Jan;11(1):86-93. doi:

    Insert

    TOP-H2B-mCherry-DD
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TOP-H2B-mCherry-DD

    Plasmid

    #96894

    Purpose

    Reporter to monitor translation of 5'TOP mRNA

    Depositor

    Tobias Meyer

    Article

    Han et al Nat Methods. 2014 Jan;11(1):86-93. doi:

    Insert

    TOP-H2B-mCherry-DD
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pKK01

    Plasmid

    #64744

    Purpose

    Yeast expression plasmid: phaA, phaB, phaC

    Depositor

    Jens Nielsen

    Article

    Kocharin et al AMB Express. 2012 Sep 25;2(1):52. doi: 1

    Insert

    phaA, phaB, phaC
    

    Expression

    Yeast

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pKK01

    Plasmid

    #64744

    Purpose

    Yeast expression plasmid: phaA, phaB, phaC

    Depositor

    Jens Nielsen

    Article

    Kocharin et al AMB Express. 2012 Sep 25;2(1):52. doi: 1

    Insert

    phaA, phaB, phaC
    

    Expression

    Yeast

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

30. pAF0466 Ef1a-Nm60_attB acceptor

    Plasmid

    #193476

    Purpose

    Acceptor plasmid for 3 plasmid recombination assay

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Nm60_attB
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. mCherry-WASH1-N-18

    Plasmid

    #55163

    Purpose

    Localization: Cytoskeleton, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    WASH1
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

32. mCherry-WASH1-N-18

    Plasmid

    #55163

    Purpose

    Localization: Cytoskeleton, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    WASH1
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

33. pEAK10-His-Myc-Kal7

    Plasmid

    #25454

    Depositor

    Betty Eipper

    Article

    Penzes et al J Biol Chem. 2000 Mar 3. 275(9):6395-403

    Insert

    Kal7
    

    Tags

    His and Myc

    Expression

    Mammalian

    Available Since

    July 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pEAK10-His-Myc-Kal7

    Plasmid

    #25454

    Depositor

    Betty Eipper

    Article

    Penzes et al J Biol Chem. 2000 Mar 3. 275(9):6395-403

    Insert

    Kal7
    

    Tags

    His and Myc

    Expression

    Mammalian

    Available Since

    July 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. lentiCRISPR v2-sgDHODH-4

    Plasmid

    #186022

    Purpose

    knock out DHODH in mammalian cells

    Depositor

    Boyi Gan

    Article

    Mao et al Nature. 2021 May;593(7860):586-590. doi:

    Insert

    Dihydroorotate Dehydrogenase (Quinone) (DHODH Human)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET30b-5M SrtA with TEV site

    Plasmid

    #86962

    Purpose

    Expresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tag

    Depositor

    Ron Bose

    Article

    Sarpong et al Anal Biochem. 2017 Mar 15;521:55-58. doi

    Insert

    S.aureus SrtA 5M
    

    Tags

    6x Histidine tag and TEV site

    Expression

    Bacterial

    Mutation

    P94R, D160N, D165A, K190E, K196T

    Promoter

    T7

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET30b-5M SrtA with TEV site

    Plasmid

    #86962

    Purpose

    Expresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tag

    Depositor

    Ron Bose

    Article

    Sarpong et al Anal Biochem. 2017 Mar 15;521:55-58. doi

    Insert

    S.aureus SrtA 5M
    

    Tags

    6x Histidine tag and TEV site

    Expression

    Bacterial

    Mutation

    P94R, D160N, D165A, K190E, K196T

    Promoter

    T7

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAV2.1-CMV-BD lacZ_3' Cer-bGHpA

    Plasmid

    #216319

    Purpose

    Split fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system).

    Depositor

    Elvir Becirovic

    Article

    Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

    Insert

    Split Cerulean + splice acceptor site
    

    Use

    AAV

    Promoter

    CMV

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV2.1-CMV-5'Cer-BDlacZ-bGHpA

    Plasmid

    #216318

    Purpose

    Split fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system).

    Depositor

    Elvir Becirovic

    Article

    Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

    Insert

    split cerulean fluorescent protein + splice donor site
    

    Use

    AAV

    Promoter

    CMV

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pIA1414

    Plasmid

    #172402

    Purpose

    Expression of SARS-COV-2 Nsp9 in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp9 (ORF1ab Synthetic)
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge