We narrowed to 29,660 results for: REP

1. pcDNA5FRT/TO-p47-Strep-HA

   Plasmid

   #113475

   Purpose

   Expression of human p47 with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   p47 (NSFL1C Human)
   

   Use

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. gRNA-eGFP-Reporter

   Plasmid

   #60719

   Purpose

   Expresses guide RNA used to activate pGL3-Basic-8x-gRNA-eGFP reporter.

   Depositor

   Charles Gersbach

   Article

   Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

   Insert

   gRNA (5'-AAAGGTCGAGAAACTGCAAA-3')
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and mouse U6

   Available since

   Jan. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMGIB-3xFLAG-reptin

   Plasmid

   #53609

   Purpose

   Retroviral expression of human reptin

   Depositor

   Steven Artandi

   Article

   Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

   Insert

   reptin (RUVBL2 Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   CDS mutated to confer resistance to shRNA. All mu…

   Promoter

   LTR

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pET21-Streptavidin-Glutamate_Tag

   Plasmid

   #46367

   Depositor

   Mark Howarth

   Article

   Fairhead et al J Mol Biol. 2013 Sep 19. pii: S0022-2836

   Insert

   Streptavidin-Glutamate_Tag
   

   Use

   Tags

   6 glutamate tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 24, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pGEX6P1-DEST-Strep2

   Plasmid

   #119753

   Purpose

   Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal Strep-II tag.

   Depositor

   Martin Reijns, Andrew Jackson

   Type

   Empty backbone

   Use

   Tags

   GST and Strep-tag II fusion tag

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA4.TO-ORF52-2xCSTREP

   Plasmid

   #136212

   Purpose

   Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF52

   Depositor

   Britt Glaunsinger

   Article

   Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

   Insert

   ORF52
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(-)-HA-SrtAstrep (pKS77)

   Plasmid

   #50030

   Purpose

   Expression of cytosolic S. pyogenes SrtA in mammalian cells

   Depositor

   Hidde Ploegh

   Article

   Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

   Insert

   SrtA(strep)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available since

   Dec. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-ccdB-TwinStrep

   Plasmid

   #214988

   Purpose

   Gateway compatible empty vector (transient mammalian expression) with a C-terminal TwinStrep tag

   Depositor

   Wade Harper

   Type

   Empty backbone

   Use

   Tags

   TwinStrep

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Dead Streptavidin-SpyCatcher

   Plasmid

   #59547

   Purpose

   pET21-DCatch encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyCatcher, for generation of chimeric SpyAvidin tetramers

   Depositor

   Mark Howarth

   Article

   Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-

   Insert

   Dead-dloop-Streptavidin-SpyCatcher
   

   Use

   Tags

   N-terminally shortened SpyCatcher

   Expression

   Bacterial

   Mutation

   PolyAsp in L3/4 loop for ion-exchange

   Promoter

   Available since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA5FRT/TO-UBXD8-Strep-HA

    Plasmid

    #113477

    Purpose

    Expression of human UBXD8 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    UBXD8 (FAF2 Human)
    

    Use

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5FRT/TO-UBE2QL1-Strep/HA

    Plasmid

    #124666

    Purpose

    Expresses UBE2QL1 with C-terminal Strep-HA tag in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

    Insert

    UBE2QL1 (UBE2QL1 Human)
    

    Use

    Tags

    2xStrep/HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGL3-p300 reporter

    Plasmid

    #11726

    Depositor

    Eileen Adamson

    Article

    Yu et al Mol Cell. 2004 Jul 2. 15(1):83-94.

    Insert

    p300 promoter and 5' UTR (EP300 )
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIgK-2.1-StreptagII

    Plasmid

    #225830

    Purpose

    Mouse IgK chain specific for chicken gamma globulin (clone 2.1). Used to produce StreptagII-tagged monoclonal antibodies for use as tag-specific ELISA standards.

    Depositor

    Gabriel Victora

    Article

    Schiepers et al Nature. 2023 Mar;615(7952):482-489. doi:

    Insert

    Mouse anti-CGG IgK chain, Streptag-II-tagged
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHS18 (mANGPTL3-Strep)

    Plasmid

    #109111

    Purpose

    Expresses StrepII-tagged mouse ANGPTL3 in mammalian cells

    Depositor

    Brandon Davies

    Article

    Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

    Insert

    ANGPTL3 (Angptl3 Mouse)
    

    Use

    Tags

    StrepII

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pcDNA5FRT/TO-UBE2QL1-CS-Strep/HA

    Plasmid

    #129513

    Purpose

    Expresses UBE2QL1 C88S (catalytically inactive mutant) with C-terminal Strep-HA tag in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

    Insert

    UBE2QL1 C88S (UBE2QL1 Human)
    

    Use

    Tags

    2xStrep/HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. eGFP L138 Reporter

    Plasmid

    #119130

    Purpose

    Acts as a reporter for APOBEC-medated single-base editing. mCherry acts as a transfection control and eGFP as a reporter.

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    mCherry and eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    eGFP L138S

    Promoter

    CMV

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5FRT/TO-UBXD7-Strep-HA

    Plasmid

    #113479

    Purpose

    Expression of human UBXD7 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    UBXD7 (UBXN7 Human)
    

    Use

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    A834C silent mutation, wildtype without stop codon

    Promoter

    CMV

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDNA4.TO-ORF57-2xCSTREP

    Plasmid

    #136217

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF57

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF57
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Fluorescent reporter for MOBE1-2

    Plasmid

    #219945

    Purpose

    Mammalian expression of 2x-deadEGFP(A111V/L202S) and aptamer-gRNAs to correct both mutations for MOBE1-2

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    CMV-EGFP(A111V/L202S); U6-A111V-gRNA-com-3'end; U6-L202S-gRNA-MS2-3'end
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    EGFP(A111V/L202S)

    Promoter

    CMV, U6

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5FRT/TO-FAF1-Strep-HA

    Plasmid

    #113486

    Purpose

    Expression of human FAF1 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    FAF1 (FAF1 Human)
    

    Use

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge