Skip to main content
Addgene

We narrowed to 26,385 results for: IRE

Showing: 9951 - 10000 of 26385 results
  1. pWZLblasti-HA-MEK1-MEK5DD

    Plasmid
    #53177
    Purpose
    Expresses HA tagged chimeric human MEK1 (nucleotides 1–201)-MEK5 (498–1344 with S311DT315D) from blasticidin resistance retroviral vector
    Insert
    MEK1 (1-201)-MEK5 (498–1344) fusion (MAP2K1 Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    MEK1 (1-201) MEK5(498–1344 with S311D/T315D)
    Promoter
    Available since
    June 4, 2014
    Availability
    Academic Institutions and Nonprofits only
  2. 289aa ELL2

    Plasmid
    #127266
    Purpose
    For in vitro translation of shorter human ELL2 from Met1. Also contains Met133I, M1381I, M186I mutations.
    Depositor
    Insert
    NH2-ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)
    Use
    In vitro translation
    Tags
    Expression
    Mutation
    Three Mets (133, 138, 186) to Ileu. Contains 289…
    Promoter
    T7
    Available since
    July 17, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. HA tagged Sph ELL2 Met133, 138, 186 to Ileu

    Plasmid
    #127271
    Purpose
    For in vitro translation of human ELL2 with Met133, 138, 186 to Ileu with HA tag after 8th Met
    Depositor
    Insert
    ELL2 Met133, 138, 186 to Ileu (ELL2 Human)
    Use
    In vitro translation
    Tags
    Expression
    Mutation
    Met133, 138, 186 to Ileu with HA tag after 8th Met
    Promoter
    T7
    Available since
    July 15, 2019
    Availability
    Academic Institutions and Nonprofits only
  4. HA tagged mid ELL2 M133, 138, 186ILeu

    Plasmid
    #127276
    Purpose
    Expresses human ELL2 with M133, 138, 186ILeu to prevent internal Met initiation peptides and contains middle HA tag that doesn't disrupt function
    Depositor
    Insert
    ELL2 (ELL2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    M133, 138, 186 to Ileu, HA tag at XbaI after Met …
    Promoter
    pEF1a
    Available since
    July 15, 2019
    Availability
    Academic Institutions and Nonprofits only
  5. EF1a_MYC Glu39Ala_P2A_Hygro_Barcode

    Plasmid
    #120512
    Purpose
    Barcoded lentiviral vector to express MYC Glu39Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
    Depositor
    Insert
    MYC Glu39Ala (MYC Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Point mutation changing Glutamic Acid to Alanine …
    Promoter
    EF1a
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
  6. pKP134 [pRS406-YBR139Wp-YBR139W(S219,D415,H474A)-GFP-ADH1t]

    Plasmid
    #106469
    Purpose
    Expresses Atg42/Ybr139w(S219,D415,H474A) with a C-terminal GFP tag in yeast cells
    Depositor
    Insert
    YBR139W(S219,D415,H474A)
    Use
    Tags
    GFP
    Expression
    Bacterial and Yeast
    Mutation
    Changed Serine 219, Aspartate 415 and Histidine 4…
    Promoter
    YBR139W
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
  7. pLuc-Optop38i-lsm

    Plasmid
    #89747
    Purpose
    Expression of light-regulated p38 inhibitor, firefly luciferase-fused, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor MK3BD3-13F
    Depositor
    Insert
    Optop38i lit-state mutant
    Use
    Luciferase
    Tags
    Firefly luciferase
    Expression
    Mammalian
    Mutation
    I539E mutation of AsLov2Jalpha
    Promoter
    CMV
    Available since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
  8. pLuc-OptoJNKi-lsm

    Plasmid
    #89746
    Purpose
    Expression of light-regulated JNK inhibitor, firefly luciferase-fused, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11
    Depositor
    Insert
    OptoJNKi lit-state mutant
    Use
    Luciferase
    Tags
    Firefly luciferase
    Expression
    Mammalian
    Mutation
    I539E mutation of AsLov2Jalpha
    Promoter
    CMV
    Available since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
  9. pLuc-OptoJNKi-dsm

    Plasmid
    #89745
    Purpose
    Expression of light-regulated JNK inhibitor, firefly luciferase-fused, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11
    Depositor
    Insert
    OptoJNKi dark-state mutant
    Use
    Luciferase
    Tags
    Firefly luciferase
    Expression
    Mammalian
    Mutation
    C450A mutation of AsLov2Jalpha
    Promoter
    CMV
    Available since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
  10. pBEST-p70a-UTR3-Nterm 6xHis mRFP K15TAG-T500

    Plasmid
    #92319
    Purpose
    Expression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR3
    Depositor
    Insert
    Optimized NTerm 6xHis tagged monomeric RFP (mRFP)
    Use
    Synthetic Biology; Cell free protein synthesis (c…
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    p70a
    Available since
    July 5, 2017
    Availability
    Academic Institutions and Nonprofits only
  11. pBEST-p70a-UTR1-Nterm 6xHis mRFP K15TAG-T500

    Plasmid
    #92318
    Purpose
    Expression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR1
    Depositor
    Insert
    Optimized NTerm 6xHis tagged monomeric RFP (mRFP)
    Use
    Synthetic Biology; Cell free protein synthesis (c…
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    p70a
    Available since
    July 5, 2017
    Availability
    Academic Institutions and Nonprofits only
  12. pBEST-p70a-UTR1-NTerm 6xHis-zmADH V86TAG-T500

    Plasmid
    #92230
    Purpose
    Expression of zmADH mutant V86TAG for ncAA incoporation using the p70a promoter and UTR1
    Depositor
    Insert
    NTerm 6xHis Tagged zmADH
    Use
    Synthetic Biology; Cell free protein synthesis (c…
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Residue V86 mutated into TAG (Amber) stop codon f…
    Promoter
    p70a
    Available since
    June 30, 2017
    Availability
    Academic Institutions and Nonprofits only
  13. pTight-9-124-BclxL

    Plasmid
    #60857
    Purpose
    2nd generation lentiviral transfer plasmid. Expresses a synthetic cluster of miR-9/9* and miR-124 fused to Bcl-xL under a doxycycline-inducible promoter
    Depositor
    Insert
    miR-9/9*-miR-124-BCLxL (BCL2L1 Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pTight
    Available since
    Dec. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
  14. pWZL-Neo-Myr-Flag-DEST

    Plasmid
    #15300
    Purpose
    a Gateway-compatible retroviral destination vector which adds a myristoylation sequence and a FLAG tag to each introduced ORF
    Depositor
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Flag and Myr
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 12, 2007
    Availability
    Academic Institutions and Nonprofits only
  15. pLHCX-ATF4 mScarlet NLS

    Plasmid
    #115970
    Purpose
    ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I (retroviral vector backbone)
    Depositor
    Insert
    activating transcription factor 4 (ATF4 Synthetic, Human)
    Use
    Retroviral
    Tags
    HA tag, c-myc NLS, and mScarlet-I
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 25, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 9951 - 10000 of 26385 results