We narrowed to 10,958 results for: AGA
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MAPK1 gRNA (BRDN0001147355)
Plasmid#77787Purpose3rd generation lentiviral gRNA plasmid targeting human MAPK1DepositorInsertMAPK1 (MAPK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 mTagBFP2 rat PGK1 shRNA
Plasmid#222869PurposeExpresses mTagBFP2 along with an shRNA against rat PGK1DepositorUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-TMPRSS2-TwinStrep_Zeo
Plasmid#192000PurposeLentiviral vector to generate TwinStrep-tagged TMPRSS2 stable expressing cell lineDepositorInsertTMPRSS2 (TMPRSS2 Human)
UseLentiviralTagsTMPRSS2ExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
FUW-Flag-Mbd3 delta1-70
Plasmid#52372PurposeLentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutantDepositorInsertMbd3 (Mbd3 Mouse)
UseLentiviralTagsflagExpressionMammalianMutationdeleted amino acids 1-70PromoterUbiquitinAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
mRFP-FKBP12-5ptpase enzyme
Plasmid#67515PurposeRecruitable human type IV 5-phosphatase enzymeDepositorUseTagsmRFPExpressionMammalianMutationINPP5E has C641A to destroy the C-terminal CAAX d…PromoterCMVAvailable sinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
FUW-Flag-Mbd3 delta 25-83
Plasmid#52373PurposeLentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutantDepositorInsertMbd3 (Mbd3 Mouse)
UseLentiviralTagsflagExpressionMammalianMutationamino acids 25-83 deletedPromoterUbiquitinAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
FUW-Flag-Mbd3 delta49-63
Plasmid#52374PurposeLentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutantDepositorInsertMbd3 (Mbd3 Mouse)
UseLentiviralTagsflagExpressionMammalianMutationdeleted amino acids 49-63PromoterUbiquitinAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP.mIKKβ
Plasmid#74412PurposeExpresses mouse eGFP-IKKβ in mammalian cellsDepositorInsertmouse IKKβ (Ikbkb Mouse)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
MAP2K4 gRNA (BRDN0001145028)
Plasmid#76650Purpose3rd generation lentiviral gRNA plasmid targeting human MAP2K4DepositorInsertMAP2K4 (MAP2K4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIS1 IL6R
Plasmid#60796PurposeLuciferase reporter to measure miR-155 mediated differential repression. Contains IL6R 3' UTR and wild-type miR-155 sitesDepositorInsertIL6R 3'UTR and wild-type miR-155 binding site (IL6R Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterthymidine kinase (HSV-TK promoter)Available sinceOct. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
LeGO-iG-CSF2RB(A455D)
Plasmid#125751PurposeLentiviral Expression Vektor with CSF2RB-A455D mutant cDNA and IRES eGFPDepositorInsertCSF2RB(A455D)
UseLentiviralTagsExpressionMutationA455D, codon optimizedPromoterSFFVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
STARR-seq luciferase INF enhancer vector_ORI_ISG15
Plasmid#99322PurposeLuciferase validation vector with ISG15 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activityDepositorInsertenhancer; chr1: 947976 -949995 (ISG15 Human)
UseLuciferase; Starr-seq luciferase validation vectorTagsExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
DHC1-AID-Clover donor (Neo)
Plasmid#158621PurposeDonor plasmid for tagging DHC1 with AID-CloverDepositorInsertDHC1 (DYNC1H1 Human)
UseCRISPR; Tagging donorTagsExpressionMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJEP325-pAAV-FullH1TO-SaCa9sgRNAi(CREB)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA
Plasmid#113702PurposeDox-inducible H1 driven SaCas9 gRNA expression cassette with a gRNA targeting CREB. Followed by an EFS driven GFP-KASH in a separate reading frame.DepositorInsertsSaCas9 gRNA Cassete
GFP
UseAAV, CRISPR, and Mouse TargetingTagsKASHExpressionMutationPromoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTT5-hSDC1-S15/25A
Plasmid#52364Purposeexpresses human Syndecan-1 serine 15 and 25 mutated to alanine (numbering excludes signal peptide) in HEK293-EBNA1 (293E) suspension culture.DepositorInsertSDC1 (SDC1 Human)
UseTagsExpressionMammalianMutationS15A, S25A and R95Q (according to numbering in Fi…PromoterCMVAvailable sinceDec. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCW57-mCherry-2A-BRD4 Iso AdelCTD
Plasmid#137722PurposeDoxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long isoform missing its C-terminal, P-TEFb interacting domain (CTD)DepositorInsertBRD4 long isoform without its C-terminal, P-TEFb interacting domain (BRD4 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationTruncated at amino acid 1328PromoterTight TRE promoterAvailable sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
FLAG-KIF5A tail only
Plasmid#166956PurposeExpresses FLAG-tagged KIF5A protein (820-1027aa) in pcDNA3.1DepositorInsertKIF5A (Kif5a Mouse)
UseTagsFLAGExpressionMammalianMutationKIF5A (820-1027aa)PromoterCMVAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTT5-hSDC1-N20
Plasmid#52355Purposeexpresses human syndecan 1 'del 1- 20' truncation mutant in HEK293-EBNA1 (293E) suspension culture.DepositorInsertSDC1 (SDC1 Human)
UseTagsExpressionMammalianMutationDeletion of SDC1 aa2-21 (according to numbering i…PromoterCMVAvailable sinceDec. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRSITEP-puro-shWRN2-C911
Plasmid#125786Purposea seed-matched control for pRSITEP-puro-shWRN2DepositorInsertshWRN2 (WRN Human)
UseRNAiTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgRNA-Cre-GpNLuc-sgmSTING
Plasmid#208386PurposeLentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine STING gene.DepositorInsertsgSTING (Sting1 Mouse)
UseLentiviral and LuciferaseTagsExpressionMammalianMutationWTPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
A3Ai E72A-Cas9n-UGI-NLS
Plasmid#109430PurposeExpresses catalytically inactive human APOBEC3A with an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.DepositorInsertApolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A E72A Catalytic Mutant (APOBEC3A Human)
UseCRISPRTagsExpressionMammalianMutationInsertion of an L1 intron into the coding sequenc…PromoterCMVAvailable sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Cas9-T2A-STOP-TdT
Plasmid#126425PurposeExpresses Cas9 but not TdT in mammalian cells; control for pcDNA-Cas9-T2A-TdT. (pTBL552)DepositorInsertCas9-T2A-STOP-TdT (DNTT Streptococcus pyogenes, Human)
UseTagsExpressionMammalianMutationalanine-6 of TdT has been changed to a STOP codonPromoterpCMVAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKHH030_sgRB1#1
Plasmid#174144PurposeLentiviral vector expressing a sgRNA against the human RB1 geneDepositorInsertRB1 (RB1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP.mIKKα
Plasmid#74413PurposeExpresses mouse eGFP-IKKα in mammalian cellsDepositorInsertmouse IKKα (Chuk Mouse)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
shERK1a-mlpx-puro
Plasmid#65228Purposeencodes a shRNA against ERK1DepositorInsertshRNA against ERK2 (MAPK1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLe6Δ -hLP-mcs/(EGFP-IresPuro-hInt)
Plasmid#64314Purposechondrocyte-specific COL11A2 promoter/enhancer lentiviral reporter vector to select iChon cellsDepositorUseLentiviralTagsEGFP and IRES-PUROExpressionMammalianMutationN/APromoterAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgArid1b#1/Cre
Plasmid#173573PurposeExpresses a Arid1b-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Arid1b (Arid1b Mouse)
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBRPy CAGGS-Mbd3-IRES-PURO
Plasmid#52376PurposeMammalian expression vector for constitutive expression of Mbd3DepositorInsertMbd3 (Mbd3 Mouse)
UseTags2xFlag (DYKDDK)ExpressionMammalianMutationPromoterCAGGSAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCW57-mCherry-2A-BRD4 Iso CdelET
Plasmid#137723PurposeDoxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long short missing its extra-terminal domain (ET)DepositorInsertBRD4 short isoform with deleted extra-terminal domain (BRD4 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationDeleted amino acids 600-682PromoterTight TRE promoterAvailable sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only
