We narrowed to 26,504 results for: vit;

1. pGEMHE:mTREK-1(K271Q)

   Plasmid

   #133270

   Purpose

   X. laevis expression vector. It will generate the mouse TREK-1 channel

   Depositor

   Dan Minor

   Article

   Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

   Insert

   Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 )
   

   Use

   Oocyte expression

   Mutation

   K271Q

   Available Since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. 432 pSG5L HA RB 567L

   Plasmid

   #10725

   Depositor

   William Sellers

   Article

   Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

   Insert

   RB 567L (RB1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   567L

   Available Since

   Sept. 27, 2005

   Availability

   Academic Institutions and Nonprofits only

3. AB.pCCL.sin.cPPT.U6.miR-132-Decoy.hPGK.GFP.WPRE

   Plasmid

   #46587

   Depositor

   Brian Brown

   Article

   Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

   Insert

   miR-132 Decoy (MIR132, Mir132 Mouse, Human)
   

   Use

   Lentiviral

   Promoter

   hU6

   Available Since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pspCas9-ABE-3'UTR-sgRNA-2xMS2

   Plasmid

   #132554

   Purpose

   Vector plasmid expressing ABEmax and sgRNA scaffold with MS2 replacing the Tetraloop and loop 2

   Depositor

   Baisong Lu

   Article

   Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

   Inserts

   ABEmax
   sgRNA, with Tetraloop and loop 2 replaced by MS2 aptamer
   

   Expression

   Mammalian

   Available Since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Zebrafish PAC (fPAC)

   Plasmid

   #129498

   Purpose

   Expresses zebrafish TMEM206. In pcDNA3.1(+) backbone

   Depositor

   Zhaozhu Qiu

   Article

   Yang et al Science. 2019 Apr 26;364(6438):395-399.

   Insert

   zebrafish PAC (pacc1 Zebrafish)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. D5_Ld_280600

   Plasmid

   #135821

   Purpose

   Expression of Leishmania donovani ectodomain in mammalian cells

   Depositor

   Gavin Wright

   Article

   Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

   Insert

   major surface protease gp63, putative,leishmanolysin, putative
   

   Tags

   biotinylation sequence and His tag

   Expression

   Mammalian

   Available Since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiCRISPRv2puro-Ptger1

   Plasmid

   #170306

   Purpose

   A knock-out vector for the mouse Ptger1

   Depositor

   Michiyuki Matsuda

   Article

   Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

   Insert

   A gRNA targeting the mouse Ptger1 gene.
   

   Use

   CRISPR and Lentiviral

   Available Since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDARMO_CMVT_3xFLAG-HUWE1_H3962D

   Plasmid

   #187131

   Purpose

   mammalian cell expression of 3xFLAG-HUWE1 H3962D

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   H3962D

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAWp63-clone32 (Opti-SpCas9)

   Plasmid

   #131736

   Purpose

   Expresses Opti-SpCas9 in mammalian cells

   Depositor

   Alan Siu Lun Wong

   Article

   Choi et al Nat Methods. 2019 Aug;16(8):722-730. doi

   Insert

   Opti-SpCas9
   

   Use

   Lentiviral

   Tags

   Flag

   Mutation

   R661A+K1003H

   Promoter

   EFS

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p-miR-148a

    Plasmid

    #65052

    Purpose

    Expression of miR148a in mammalian cells

    Depositor

    Cristina Fillat

    Article

    Bofill-De Ros et al Mol Ther. 2014 Sep;22(9):1665-77. doi: 1

    Insert

    hsa-miR-148a (pre-miRNA) (MIR148A Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ABE8e(TadA-8e K20A R21A)

    Plasmid

    #138496

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e K20A R21A)-nSpCas9
    

    Expression

    Mammalian

    Mutation

    TadA-8e K20A R21A

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. LLP250 pEF1a-TALE-SunTag

    Plasmid

    #100939

    Purpose

    Empty TALE vector fused to SunTagx10 driven by EF1a promoter, with 3xHA tag, no PURO gene

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    TALE insertion site, SunTag array (10xGCN4)
    

    Use

    TALEN

    Tags

    3xHA

    Expression

    Mammalian

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-FRB:Akt1(D274A)

    Plasmid

    #86634

    Purpose

    Expresses FRB-tagged human Akt1(D274A) kinase-inactive mutant

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    FRB-Akt1(D274A) (AKT1 Human)
    

    Expression

    Mammalian

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MDH1-shDusp5-2-H2Kb2

    Plasmid

    #17853

    Depositor

    Chang-Zheng Chen

    Article

    Li et al Cell. 2007 Apr 6. 129(1):147-61.

    Insert

    shDusp5 (DUSP5 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-3xFLAG-Puro PAK5 K478R K469R

    Plasmid

    #167180

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 K478R K469R

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 K478R K469R
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX-4T-1-3xMyc-ERK2(K52R)

    Plasmid

    #129228

    Purpose

    Encodes Myc tagged human ERK2(K52R) for purification by GST affinity

    Depositor

    Kevin Janes

    Article

    Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

    Insert

    ERK2(K52R) (Mapk1 Rat)
    

    Tags

    GST and Myc

    Expression

    Bacterial

    Mutation

    Changed Lysine 52 to Arginine

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. VRER variant

    Plasmid

    #138561

    Purpose

    Expresses human codon-optimized SpCas9-VRER and blasticidin resistance: EFS promoter-VRER-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    VRER
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    D1135V, G1218R, R1335E, T1337R

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. IBMc326

    Plasmid

    #161949

    Purpose

    Contains Type IIS restriction site free elements for expression vectors in IBM. Expression vector to generate ORF insertion library in IBM on ECF20

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-5)-BsaI-BsaI-ECF20(187-193)-Ter
    

    Expression

    Bacterial

    Promoter

    PBAD

    Available Since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MSCV-miR30_shBrd9_1116-PGK-NeoR-IRES-mCherry

    Plasmid

    #75133

    Purpose

    Retroviral expression plasmid encoding shBrd9_1116 (targeting murine Brd9)

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    shBrd9_1116
    

    Use

    Retroviral

    Available Since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

20. mCardinal-MannII-N-10

    Plasmid

    #56167

    Purpose

    Localization: Mannosidase/Golgi, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    MannII
    

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    aa 1-112 of MannII

    Promoter

    CMV

    Available Since

    March 4, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

21. pmCherry-PH1(K14A)

    Plasmid

    #86600

    Purpose

    Expresses mCherry-tagged PH(K14A) domain of human Akt1

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    K14A mutant of Akt1 PH domain (AKT1 Human)
    

    Expression

    Mammalian

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA5 FRT/TO Plk4 KD ∆24-mCherry

    Plasmid

    #80272

    Purpose

    mammalian expression plasmid for kinase dead Plk4 with multi-phospho degron deleted

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    Plk4 (PLK4 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    deletion of multi-phospho degron (amino acids 282…

    Promoter

    CMV

    Available Since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. LentiCRISPRv2puro-Ptgs2_Balbc

    Plasmid

    #170301

    Purpose

    A knock-out vector for the mouse Ptgs2 in Balb/c

    Depositor

    Michiyuki Matsuda

    Article

    Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

    Insert

    A gRNA targeting the mouse tyrosinase gene.
    

    Use

    CRISPR and Lentiviral

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBEG R2-eGFP-L3 #BGV201

    Plasmid

    #48979

    Purpose

    Supplies the selection marker (eGFP) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Insert

    eGFP
    

    Use

    Gateway cloning vector

    Tags

    IRES (strong)

    Promoter

    NA

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

25. pcDNA3.1(+)-FluoSTEP-EKAR

    Plasmid

    #181858

    Purpose

    Genetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    FluoSTEP-EKAR
    

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AB.pCCL.sin.cPPT.U6.miR-223-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46601

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-223 Decoy (MIR223, Mir223 Mouse, Human)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-DR1-NRSE-RFP

    Plasmid

    #22927

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

    Insert

    peroxisome proliferator activated receptor gamma binding site promoter with neuron-restrictive silencer element with minimal CMV
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBEG R2-iNeo-L3 #BGV207

    Plasmid

    #48986

    Purpose

    Supplies the selection marker (neomycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Insert

    neomycin resistance
    

    Use

    Gateway cloning vector

    Tags

    IRES (weak)

    Promoter

    NA

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

29. pJ207 Delta-cpc-KanR

    Plasmid

    #51468

    Purpose

    Deletes the cpc operon in Synechocystis and confers kanamycin resistance

    Depositor

    Anastasios Melis

    Article

    Kirst et al Biochim Biophys Acta. 2014 Oct;1837(10):

    Insert

    Replacing the cpc operon with kanamycin resistance
    

    Use

    Synthetic Biology

    Promoter

    Synechocystis endogenous cpc-operon promoter

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-3)

    Plasmid

    #159658

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Promoter

    Ef1a

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-2)

    Plasmid

    #159657

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Promoter

    Ef1a

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRSETa-Amrose v1

    Plasmid

    #79537

    Purpose

    pRSETa (Invitrogen) plasmid expressing Amrose variant 1

    Depositor

    Randolph Caldwell

    Article

    Schoetz et al PLoS One. 2014 Sep 5;9(9):e107069. doi:

    Insert

    Amrose
    

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. vsv-CenpB 1-166-Sgo1 N61I-GFP

    Plasmid

    #108499

    Purpose

    expression of vsv-CenpB 1-166-Sgo1 N61I-EGFP (no PP2A binding)

    Depositor

    Susanne Lens

    Article

    Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

    Insert

    CenpB-Shugoshin 1 (SGO1 Human)
    

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Mutation

    CenpB 1-166 fusion before Sgo1, no PP2A binding N…

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3myc-PR-LBD

    Plasmid

    #89113

    Purpose

    mammalian expression of human progesterone receptor ligand binding domain amino acid residues 632-933 with N-terminal myc tag

    Depositor

    Elizabeth Wilson

    Article

    Liu et al J Biol Chem. 2011 Aug 26;286(34):29951-6

    Insert

    human progesterone receptor ligand binding domain (PGR Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    contains Ligand Binding Domain (632-933)

    Promoter

    CMV

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

35. AB.pCCL.sin.cPPT.U6.miR-146a-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46590

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-146a Decoy (MIR146A, Mir146 Mouse, Human)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

36. pFBD-Akt1(K14A)

    Plasmid

    #86562

    Purpose

    Expresses human full-length Akt1(K14A) mutant

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Tags

    10xHis

    Expression

    Insect

    Mutation

    K14A mutation in the PH domain

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pVLAD6-mmPtch1-B-SBP

    Plasmid

    #120886

    Purpose

    BacMam vector for Ptch1-B expression

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    ptch1 (Ptch1 Mouse)
    

    Tags

    SBP

    Expression

    Mammalian

    Mutation

    deleted amino acids 620-643, truncated at 1291

    Promoter

    CMV

    Available Since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Sumo3 PolQM1 deltains3

    Plasmid

    #78459

    Purpose

    Inducible expression in E. coli

    Depositor

    Sylvie Doublie, Susan Wallace

    Article

    Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

    Insert

    Polymerase theta (POLQ Human)
    

    Tags

    His and SUMO3

    Expression

    Bacterial

    Mutation

    delta 1-1791; delta 2497-2529

    Promoter

    T7

    Available Since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

39. LentiCRISPRv2puro-Gnaq

    Plasmid

    #170302

    Purpose

    A knock-out vector for the mouse Gnaq

    Depositor

    Michiyuki Matsuda

    Article

    Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

    Insert

    A gRNA targeting the mouse Gnaq gene.
    

    Use

    CRISPR and Lentiviral

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. LZRSpBMN-­Z‐GFP‐Eps8

    Plasmid

    #74935

    Purpose

    Expresses mouse Eps8 fused to GFP

    Depositor

    Giorgio Scita

    Article

    Frittoli et al Immunity. 2011 Sep 23;35(3):388-99. doi:

    Insert

    Eps8 (Eps8 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only