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Plasmid#72619PurposeExpresses two gRNAs targeting GFPDepositorInsertgRNAs toward GFP
UseTagsExpressionMammalianMutationPromoterU6 (gRNA1) and H1 (gRNA2)Available sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP (AAV8)
Viral Prep#137141-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP (#137141). In addition to the viral particles, you will also receive purified pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP plasmid DNA. nEF-driven, Flp-dependent expression of ChR2(E123T/T159C)-EYFP (inhibited in presence of Cre) for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoternEFTagsEYFP (Flp-dependent)Available sinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Tet-MKK6(DD)-Puro
Plasmid#86094PurposeTet/Dox inducible (TetR) constitutively active mutant MKK6/MAP2K6 in lentiviral vectorDepositorInsertMKK6 (MAP2K6 Human)
UseLentiviralTagsMyc tagExpressionMammalianMutationSerine 207 and Threonine 211 both changed to Aspa…PromoterCMV/TetOAvailable sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
mPA-GFP-TOMM20-N-10
Plasmid#57158PurposeLocalization: Outer Mitochondrial Membrane, Excitation: 400 / 504, Emission: 515 / 517DepositorInsertTOMM20 (TOMM20 Human)
UseTagsmPA-GFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
PA-GFP Golgi
Plasmid#57164PurposeLocalization: Golgi, Excitation: 400 / 504, Emission: 515 / 517DepositorInsertGolgi (B4GALT1 Human)
UseTagsPA-GFP GolgiExpressionMammalianMutationPromoterCMVAvailable sinceJan. 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDIRECT_23C
Plasmid#91140PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:barDepositorInsertEngineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-mRuby2
Plasmid#111250PurposeBroad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mRuby2 (codon optimized for P. fluorescens)DepositorInsertmRuby2
UseTagsExpressionBacterialMutationmRuby2 is codon optimized for expression in P. fl…PromoterPcAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUCP30T-mKo1
Plasmid#78477PurposeBroad range bacterial fluorescence labelingDepositorInsertmKO1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG_Xph20-SNAPf-CCR5TC
Plasmid#135537PurposeEncodes a specific PSD-95 binder (Xph20) fused to SNAPf, CCR5TC zinc finger, and KRAB(A) transcriptional repressor domainDepositorInsertXph20
UseTagsSNAPfExpressionMammalianMutationPromoterCAGAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-Src-Y530F
Plasmid#140319PurposeExpresses Flag-tagged human Src-Y530F in mammalian cells for structure-function studiesDepositorInsertSrc mutant (SRC Human)
UseTags2X FlagExpressionMammalianMutationY530FPromoterCMVAvailable sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
BFP-RanQ69L
Plasmid#163438PurposeMammalian expression of RanQ69L with tag-BFP; contains miniAux domain for degredationDepositorInsertRanQ69L (RAN Human)
UseTagstag-BFPExpressionMammalianMutationQ69LPromoterCMVAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
mPA-GFP-H2B-6
Plasmid#57137PurposeLocalization: Nucleus/Histones, Excitation: 400 / 504, Emission: 515 / 517DepositorInsertH2B (H2BC11 Human)
UseTagsmPA-GFPExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceJan. 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ubiq-Cas9.874W
Plasmid#112686PurposeExpress hSpCas9 under Ubiquitin-63E promoterDepositorInserthSpCas9
UseTags3x FLAG, EGFP, and NLSExpressionInsectMutationHumanized Cas9 bacterial sequencePromoterUbiquitin-63E promoterAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG_Xph15-SNAPf-CCR5TC
Plasmid#135536PurposeEncodes a specific PSD-95 binder (Xph15) fused to SNAPf, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domainDepositorInsertXph15
UseTagsSNAPfExpressionMammalianMutationPromoterCAGAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5X-ELP[AV-60]
Plasmid#67012PurposeExpresses malE-ELP[AV-60] in E. coliDepositorInsertmalE-ELP[AV-60]
UseTagsHis6 and malE-Factor XaExpressionBacterialMutationPromotertacAvailable sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTH10
Plasmid#188783PurposeCarries a NAT marker and C. albicans RBT5 promoter in a pGEM-T backboneDepositorInsertRBT5 promoter
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceSept. 1, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti-puro /BRAF-V600E
Plasmid#131724PurposeMammalian Expression of Flag tagged BRAFDepositorInsertBRAF (BRAF Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationV600EPromoterCMVAvailable sinceFeb. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
cpLID-micro
Plasmid#168995Purposecircularly permuted LOV2 based optical dimerization system (ssrA-cpLOV2 and sspB)DepositorInsertmCh-sspB-T2A-cpLID-CAAX
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Galphai2-mTurquoise2
Plasmid#69621PurposeThe heterotrimeric G-protein subunit, Gαi isoform 2, tagged with mTurquoise2DepositorInsertGalphai2-mTurquoise2
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
nos-Cas9.874Z1
Plasmid#112685PurposeExpress hSpCas9 under nanos promoterDepositorInserthSpCas9
UseTags3x FLAG, EGFP, and NLSExpressionInsectMutationHumanized Cas9 bacterial sequencePromoternanosAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only