167,795 results

1. pBR HPV31 9E

   Plasmid

   #221009

   Purpose

   Human papillomavirus 31 isolate 9E, complete genome cloned in pBR322

   Depositor

   Alison McBride

   Insert

   Human papillomavirus type 31 genome 9E isolate
   

   Use

   Cloning vector

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-CMV-hXRCC1-mCherry-Hygro

   Plasmid

   #176532

   Purpose

   mCherry fused to the C-terminus of XRCC1 & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Wilk et al Sci Rep. 2020 Jan 20;10(1):651. doi: 10.

   Insert

   X-Ray Repair Cross Complementing 1 (XRCC1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCherry-VAPB

   Plasmid

   #108126

   Purpose

   ER marker

   Depositor

   Gerry Hammond

   Article

   Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

   Insert

   mCherry:VAPB (VAPB Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEM705 Gsi (Cys)

   Plasmid

   #109373

   Purpose

   GsX chimera with Gαi C-terminus (Pertussis toxin sensitive)

   Depositor

   Robert Lucas

   Article

   Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

   Insert

   Gsi chimera
   

   Tags

   None

   Expression

   Mammalian

   Mutation

   Cys at position 391 (Pertussis Toxin Sensitive)

   Promoter

   CAG

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. CCR5_gRNA_pX459

   Plasmid

   #192268

   Purpose

   gRNA expression for CRISPR Genome Editing of CCR5

   Depositor

   Shantanu Chowdhury

   Article

   Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

   Insert

   CCR5 guide RNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. TERTpWT_Gluc_CCR5

   Plasmid

   #192270

   Purpose

   Donor template for insertion of hTERT promoter driven Gaussia luciferase reporter

   Depositor

   Shantanu Chowdhury

   Article

   Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

   Insert

   Promoter Reporter Insert within donor template
   

   Use

   CRISPR

   Promoter

   TERT promoter driving expression of gaussia

   Available Since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. RC(WW)-LgBit

   Plasmid

   #208931

   Purpose

   Recognition domain for in vitro MAPK-activity sensor

   Depositor

   Attila Reményi

   Article

   Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

   Insert

   WW domain of PIN1 (PIN1 Human)
   

   Tags

   HISx6, LgBit, and MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJT302 Ec03 Lenti pEF-attB LSR GFP

   Plasmid

   #184945

   Purpose

   Lentiviral delivery of recombinase landing pad

   Depositor

   Patrick Hsu, Michael Bassik, Ami Bhatt, Lacra Bintu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Ec03 Lenti pEF1a-attB LSR GFP
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCas9-CAT

   Plasmid

   #80323

   Purpose

   Encodes Cas9 and chloramphenicol acetyltransferase (CAT)

   Depositor

   Sebastian Lourido

   Article

   Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

   Inserts

   Cas9
   Chloramphenicol acetyltransferase
   

   Use

   CRISPR

   Tags

   3X FLAG and NLS

   Promoter

   TgTUB1

   Available Since

   Sept. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDG330

    Plasmid

    #100898

    Purpose

    SpCas9 with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-FLAG-mCherry-C1-centrin-1

    Plasmid

    #73333

    Purpose

    Lentiviral vector encoding centrin-1 with N-terminal FLAG and mCherry tags

    Depositor

    Manuel Thery

    Insert

    centrin-1 (CETN1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5FRT/TO-UBXD4-Strep-HA

    Plasmid

    #113480

    Purpose

    Expression of human UBXD4 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    UBXD4 (UBXN2A Human)
    

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. LRP6 deltaN-pCS2-VSVG

    Plasmid

    #27281

    Depositor

    Xi He

    Article

    Tamai et al Mol Cell. 2004 Jan 16. 13(1):149-56.

    Insert

    low density lipoprotein receptor-related protein 6 (LRP6 Human)
    

    Use

    Xenopus injection

    Tags

    VSVG

    Mutation

    contains amino acid residues 1370–1613 (extracell…

    Available Since

    Jan. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPGK-T7/2-CD44v6 (fl)

    Plasmid

    #137816

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v6 (fl) (CD44 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    R296K in the V6 region [Please see depositor comm…

    Promoter

    PGK

    Available Since

    June 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJT303 Ec04 Lenti pEF-attB LSR GFP

    Plasmid

    #184946

    Purpose

    Lentiviral delivery of recombinase landing pad

    Depositor

    Patrick Hsu, Michael Bassik, Ami Bhatt, Lacra Bintu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Ec04 Lenti pEF1a-attB LSR GFP
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLIK MEK5-DD-mRFP1 neo

    Plasmid

    #47548

    Purpose

    Lentiviral expression vector for an inducible mRFP1-tagged constitutively active MEK5 mutant

    Depositor

    Kevin Janes

    Article

    Jensen et al Cell Host Microbe. 2013 Jan 16;13(1):67-

    Insert

    MEK5-DD-mRFP1 (MAP2K5 Human)
    

    Use

    Lentiviral

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    S311D, T315D

    Available Since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pLKO5.U6.DR130(CasRX)_Non-targeting sgRNA.EFS.tRFP657

    Plasmid

    #212962

    Purpose

    Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.

    Depositor

    Roland Rad

    Article

    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

    Insert

    DR1_CasRx_non-targeting spacer
    

    Use

    CRISPR and Lentiviral

    Tags

    TagRFP657

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUdO2c

    Plasmid

    #210231

    Purpose

    medium-copy plasmid for bacterial expression

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. NG-ABEmax

    Plasmid

    #124163

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    TadA-TadA(evo)-Cas9-NG
    

    Expression

    Mammalian

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMJA089

    Plasmid

    #128518

    Purpose

    Expresses human Lysozyme in Pichia pastoris

    Depositor

    Marcos Alcocer

    Article

    Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. d

    Insert

    Artificial gene for human lysozyme (LYZ Human)
    

    Expression

    Yeast

    Promoter

    AOX1

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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