Addgene

We narrowed to 23,340 results for: Nov;

Showing: 6211 - 6240 of 23340 results

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LentiCRISPRv2 NT sgRNA5

Plasmid

#164930

Purpose

Expresses Non-targeting sgRNA5 control in mammalian cells

Depositor

Ji Luo

Article

Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

Insert

Non-targeting sgRNA5 (verified for knockout)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EFS promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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SCML2_3

Plasmid

#220878

Purpose

Baculovirus expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Article

Nady et al J Mol Biol. 2012 Nov 9;423(5):702-18. do

Insert

SCML2 (SCML2 Human)

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-FLAG/HA-DCP1

Plasmid

#215843

Purpose

Expression of FLAG/HA-DCP1

Depositor

Kumiko Ui-Tei

Article

Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

Insert

Human DCP1 (DCP1B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-syt1 (96-421)

Plasmid

#213619

Purpose

Bacterial expression of SUMO-tagged rat syt1 (96-421)

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt1 (96-421) (Syt1 Rat)

Use

Tags

SUMO

Expression

Bacterial

Mutation

Promoter

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-syt7 (136-403)

Plasmid

#213621

Purpose

Bacterial expression of SUMO-tagged mouse syt7 (136-403)

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt7 (136-403) (Syt7 Mouse)

Use

Tags

SUMO

Expression

Bacterial

Mutation

Promoter

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-syt1 5W

Plasmid

#213623

Purpose

Bacterial expression of SUMO-tagged rat syt1 5W

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt1 5W (Syt1 Rat)

Use

Tags

SUMO

Expression

Bacterial

Mutation

M173W, F231W, F234W, C277A, V304W, Y364W, I367C

Promoter

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-syt1 s7+

Plasmid

#213624

Purpose

Bacterial expression of SUMO-tagged rat syt1 s7+

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt1 s7+ (Syt1 Rat)

Use

Tags

SUMO

Expression

Bacterial

Mutation

L171K, H198K, K236R, C277A, N333R, Y364K, I 367C,…

Promoter

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-syt7 C2AB - 4A

Plasmid

#213625

Purpose

Bacterial expression of SUMO-tagged mouse syt7 C2AB - 4A

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt7 C2AB - 4A (Syt7 Mouse)

Use

Tags

SUMO

Expression

Bacterial

Mutation

F167A, F229A, I298A, L361A

Promoter

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-syt7 C2AB-2A

Plasmid

#213627

Purpose

Bacterial expression of SUMO-tagged mouse syt7 C2AB-2A

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt7 C2AB-2A (Syt7 Mouse)

Use

Tags

SUMO

Expression

Bacterial

Mutation

I298, L361A

Promoter

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mGCC2-FLAG

Plasmid

#214166

Purpose

Transient expression of GCC2

Depositor

Nan Yan

Article

Tu et al Nat Commun. 2022 Nov 15;13(1):6977. doi:

Insert

GCC2 (Gcc2 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-S437A

Plasmid

#208803

Purpose

Xenopus laevis His6-Myc-DONSON (S437A)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

S437A

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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LLP782_Traptavidin-KRAB-CO

Plasmid

#211775

Purpose

AviTag counterpart binding domain, Traptavidin, fused to transcriptional repressor KRAB, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

SpyCatcher-DNMT3A

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect DNMT3A…

Promoter

pEF1a and pSV40

Available since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMuLE (L1-L4) 6X PRE

Plasmid

#206168

Purpose

pMuLE L1-L4 entry plasmid containing 6 consensus PGR bindings sites upstream of a minimal 2YBTATA promoter

Depositor

Renee van Amerongen

Article

Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

Insert

6X PRE

Use

Gateway cloning entry clone

Tags

Expression

Mutation

Promoter

2YBTATA

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMuLE (L1-L4) 4X PRE

Plasmid

#206167

Purpose

pMuLE L1-L4 entry plasmid containing 4 consensus PGR bindings sites upstream of a minimal 2YBTATA promoter

Depositor

Renee van Amerongen

Article

Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

Insert

4X PRE

Use

Gateway cloning entry clone

Tags

Expression

Mutation

Promoter

2YBTATA

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMuLE (L1-L4) 2X PRE

Plasmid

#206166

Purpose

pMuLE L1-L4 entry plasmid containing 2 consensus PGR bindings sites upstream of a minimal 2YBTATA promoter

Depositor

Renee van Amerongen

Article

Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

Insert

2X PRE

Use

Gateway cloning entry clone

Tags

Expression

Mutation

Promoter

2YBTATA

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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SpyCatcher003(K31TAG)-Talin rod-mScarletI

Plasmid

#210022

Purpose

Expression of SpyCatcher003(K31TAG)-Talin rod(434-2541)-mScarletI in mammalian cells

Depositor

Vesa Hytönen

Article

Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

Insert

SpyCatcher003(K31TAG)-Talin rod(434-2541)-mScarletI

Use

Tags

C-terminal mScarletI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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LLP785_Traptavidin-DNMT3A-CO

Plasmid

#211778

Purpose

AviTag counterpart binding domain, Traptavidin, fused to DNA methyltransferase DNMT3A, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

Traptavidin-EZH2

Use

Tags

3xV5

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect EZH2 e…

Promoter

pEF1a and pSV40

Available since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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LLP781_SnoopCatcher-KRAB-CO

Plasmid

#211774

Purpose

SnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional repressor KRAB, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

Traptavidin-KRAB

Use

Tags

3xV5

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect KRAB e…

Promoter

pEF1a and pSV40

Available since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SUMO-A'-l3-A'

Plasmid

#209118

Purpose

Bacterial expression of CC-GEMS ligand SUMO-A'-l3-A'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l3-A'

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SUMO-A'-l2-G'

Plasmid

#209122

Purpose

Bacterial expression of CC-GEMS ligand SUMO-A'-l2-G'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l2-G'

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SUMO-A'-l2-B'

Plasmid

#209121

Purpose

Bacterial expression of CC-GEMS ligand SUMO-A'-l2-B'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l2-B'

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SUMO-A'-l1-A'

Plasmid

#209116

Purpose

Bacterial expression of CC-GEMS ligand SUMO-A'-l1-A'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l1-A'

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SUMO-A'-l4-G

Plasmid

#209123

Purpose

Bacterial expression of CC-GEMS ligand SUMO-A'-l4-G

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l4-G

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SUMO-A'-l2-A'

Plasmid

#209117

Purpose

Bacterial expression of CC-GEMS ligand SUMO-A'-l2-A'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l2-A'

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLeo-A-a2-GEMS(PLCG)

Plasmid

#209114

Purpose

Transient mammalian expression of the CC functionalized GEMS receptor A-a2-GEMS (PLCG)

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

A-a2-GEMS(PLCG)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCOA-Leu-FvPPT1

Plasmid

#179923

Purpose

Expression vector for heterologous expression of a polyketide synthase in S. cerevisiae. Carries and empty insertion site and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1

Depositor

Jens Laurids Sørensen

Article

Bejenari et al J Microbiol Biol Educ. 2023 Nov 27;24(3)

Insert

FvPPT1

Use

Tags

Expression

Yeast

Mutation

Codon optimized for expression in Saccharomyces c…

Promoter

Yarrowia lipolytica EXP1

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 DomI + DomII pcDNA3

Plasmid

#206094

Purpose

expression of the N-terminus, Domain I, Domain II and the II-III loop (amino acids 1-1154) of rabbit Cav2.2 calcium channel

Depositor

Annette Dolphin

Article

Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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phROSA26-Tet-HA-JLP_V55Q

Plasmid

#208771

Purpose

Tetracycline inducible expression vector for HA-JLP_V55Q mutant protein, used as a donor plasmid for HA-JLP_V55Q knock-in at the human ROSA26 locus

Depositor

Katsuji Yoshioka

Article

Suzuki et al Genes Cells. 2023 Nov 14. doi: 10.1111/g

Insert

JLP (SPAG9 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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10X PRE TK luc

Plasmid

#206162

Purpose

Luciferase reporter construct containing 10 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350

Depositor

Renee van Amerongen

Article

Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

Insert

10X PRE TK luciferase

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

min TK

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
8X PRE TK luc

Plasmid

#206161

Purpose

Luciferase reporter construct containing 8 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350

Depositor

Renee van Amerongen

Article

Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

Insert

8X PRE TK luciferase

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

min TK

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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