We narrowed to 35,144 results for: cas

1. mAMCase_CatD_6xHis_pTwistCMV

   Plasmid

   #200228

   Purpose

   Expresses mouse Acidic Mammalian Chitinase catalytic domain with C-terminal 6xHis tag in pTwist CMV vector

   Depositor

   James S Fraser

   Article

   Diaz et al Elife. 2024 Jun 17;12:RP89918. doi: 10.7

   Insert

   Acidic Mammalian Chitinase (Chia1 Mouse)
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   S354F

   Promoter

   CMV

   Available since

   Sept. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. EC2_10_dCas9_VPR_HC_sgRNA

   Plasmid

   #163712

   Purpose

   Yeast high copy plasmid with dCas9, sgRNA expression cassette and VPR activation domains

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Inserts

   dCas9
   VPR
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. AsCas12a

   Plasmid

   #188492

   Purpose

   Expresses FLAG tagged AsCas12a

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   Cas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDonor_AcCAST_kanR

   Plasmid

   #127925

   Purpose

   Kanamycin resistance donor for AcCAST

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

   Insert

   KanR
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. p130Cas

   Plasmid

   #186131

   Purpose

   For expression of recombinant p130Cas in SF9 cells.

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   BCAR1 (Bcar1 Rat)
   

   Use

   Tags

   His6

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin promoter

   Available since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. NAX_CAGGS_CAS9_VPR_Puro

   Plasmid

   #167895

   Purpose

   PiggyBac compatible plasmid expressing Cas9-VPR

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   Cas9-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. VPR_dCas9

   Plasmid

   #134601

   Purpose

   Express CHO codon-optimized dCas9 fused to VPR for CRISPR activation. 2A linked ZsGreen1 marker

   Depositor

   Lasse Ebdrup Pedersen

   Article

   Karottki et al Biotechnol Bioeng. 2020 Feb;117(2):593-5

   Insert

   VPR-dCas9-2A-ZsGreen-DR
   

   Use

   Tags

   VPR and ZsGreen1-DR

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMEG_YLCas9_empty

   Plasmid

   #117826

   Purpose

   CRISPR/Cas9 plasmid for GoldenMOCS in Y. lipolytica

   Depositor

   Michael Sauer

   Article

   Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

   Insert

   YlpTEF_hCas9_YlCyc1TT
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. SpCas9_sgRNA_expression_in_pBluescript

   Plasmid

   #122089

   Purpose

   U6 driven SpCas9 sgRNA expression vector for cloning own guides

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   SpCas9 tracr (NEWENTRY Synthetic, S. pyogenes)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Nme2Cas9_pCDest

    Plasmid

    #119923

    Purpose

    Nme2Cas9 CMV-driven mammalian expression plasmid

    Depositor

    Erik Sontheimer

    Article

    Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

    Insert

    Nme2Cas9
    

    Use

    Tags

    NLS-3xHA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCas9_sgRNA_0

    Plasmid

    #70763

    Purpose

    expresses Ustilago maydis codon-optimized Cas9, contains U. maydis U6 promoter, is self-replicating

    Depositor

    Regine Kahmann

    Article

    Schuster et al Fungal Genet Biol. 2015 Sep 11. pii: S10

    Inserts

    U6 promoter
    cas9
    tnos terminator
    

    Use

    Self-replicating in ustilago maydis, conferring c…

    Tags

    N-terminal NLS, C-terminal HA-tag +NLS

    Expression

    Mutation

    Streptococcus pyogenes gene codon-optimized for U…

    Promoter

    U6 from Ustilago maydis and synthetic otef promot…

    Available since

    Nov. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCas9

    Plasmid

    #162606

    Purpose

    Pre-express Cas9 in yeast

    Depositor

    Lars Steinmetz

    Article

    Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

    Insert

    Tef1-Cas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCas2C

    Plasmid

    #82374

    Purpose

    dCas9 expressed from aTc-inducible promoter in acsA, C RBS: TGGACA

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PEZ3

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCas34

    Plasmid

    #82386

    Purpose

    sgRNA targeting YFP expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PEZ3

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCas3

    Plasmid

    #229538

    Purpose

    pMV_hyg encoding Cas3(wt) and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning.

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Insert

    Cas3
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae

    Promoter

    Available since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHelper_AcCAST

    Plasmid

    #127923

    Purpose

    Expresses AcCAST and a crRNA targeting PSP1

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    AcTnsB, AcTnsC, AcTniQ, AcCas12k
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX-evoCas9

    Plasmid

    #107550

    Purpose

    Expresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cells

    Depositor

    Anna Cereseto

    Article

    Casini et al Nat Biotechnol. 2018 Jan 29. pii: nbt.40

    Insert

    Humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    M495V/Y515N/K526E/R661Q

    Promoter

    CBh

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPbcas13b

    Plasmid

    #89906

    Purpose

    Bacterial expression for pbCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.

    Depositor

    Feng Zhang

    Article

    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

    Insert

    Cas13b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pdCASclos

    Plasmid

    #73640

    Purpose

    Transcriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824

    Depositor

    Sheng Yang

    Article

    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

    Inserts

    dCas9
    sgRNA to spo0A
    

    Use

    E.coli-clostridium shuttle vector

    Tags

    Expression

    Mutation

    D10A, H840A

    Promoter

    Pj23119 and ptb

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. Casp1

    Plasmid

    #98849

    Purpose

    Expresses murine Casp1 in mammalian cells

    Depositor

    Peter Keyel

    Article

    Shi et al Front Immunol. 2017 May 8;8:522. doi: 10

    Insert

    Casp1 (Casp1 Mouse)
    

    Use

    Tags

    No fusion proteins or tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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