We narrowed to 15,978 results for: LEA

1. GALTA-c001

   Plasmid

   #79645

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   GALT (GALT Human)
   

   Tags

   6x His and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGBW-m4046323

   Plasmid

   #149100

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp3

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp3 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;MBP

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pEG408

   Plasmid

   #40086

   Depositor

   Geraldine Seydoux

   Article

   Griffin et al Cell. 2011 Sep 16;146(6):955-68.

   Insert

   Mex-5 (aa 345 - 468) (mex-5 Nematode)
   

   Tags

   Dendra2 PFAF, S-peptide, and TEV protease cleavag…

   Expression

   Worm

   Mutation

   contains aa 345 - 468

   Promoter

   pie-1

   Available Since

   Aug. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pMAH-Golgi-bapaUGAc

   Plasmid

   #201675

   Purpose

   boronic acid- and peptide-assisted UDP-GlcNAc sensor in Golgi

   Depositor

   Huiwang Ai

   Article

   Zhang et al ACS Sens. 2023 Jul 22. doi: 10.1021/acss

   Insert

   Golgi-bapaUGAc
   

   Tags

   Golgi leading sequence

   Expression

   Mammalian

   Promoter

   CMV SP6

   Available Since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGBW-m4134165

   Plasmid

   #152085

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;3xFlag

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available Since

   May 20, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pGBW-m4258306

   Plasmid

   #154795

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 E (envelope)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;V5

   Expression

   Mammalian

   Mutation

   H. sapiens recode 1

   Promoter

   CMV

   Available Since

   July 30, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pGBW-m4046348

   Plasmid

   #149108

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp6

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp6 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pBXNH3A

   Plasmid

   #47075

   Purpose

   FX cloning E.coli expression vector with araBAD promoter and N-terminal 10x His, 3C protease cleavage site and Avi-tag

   Depositor

   Eric Geertsma, Raimund Dutzler

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Tags

   3C protease site (PreScission site), Avi-tag, and…

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pOPTM p62 PB1 ZZ (1-167)

   Plasmid

   #210429

   Purpose

   Bacterial expression of MBP-PB1 ZZ (1-167) with TEV cleavage site

   Depositor

   Carsten Sachse

   Article

   Tarafder et al Methods Mol Biol. 2019;1880:3-15. doi: 1

   Insert

   p62 PB1 ZZ (1-167) (NUP62 Human)
   

   Tags

   MBP

   Expression

   Bacterial

   Available Since

   Feb. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJA50

    Plasmid

    #59931

    Purpose

    gRNA for cleavage at unc-58(e665) locus in C elegans

    Depositor

    Andrew Fire

    Article

    Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

    Insert

    unc-58(e665) gRNA
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBXNAC3GH

    Plasmid

    #47073

    Purpose

    FX cloning E.coli expression vector with araBAD promoter, N-terminal Avi-tag, C-terminal 3C protease cleavage site, GFP and 10x His tags

    Depositor

    Eric Geertsma, Raimund Dutzler

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Tags

    3C protease site (PreScission site), Avi-tag, GFP…

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGBW-m4133326

    Plasmid

    #152113

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;HA

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    May 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pGBW-m4046310

    Plasmid

    #149022

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 M (membrane) (M Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pGBW-m4046228

    Plasmid

    #145400

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF7a protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF7a protein (ORF7a Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pEPQD0CM0281

    Plasmid

    #162302

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, [S-tag]-[TEV cleavage site]
    

    Use

    Synthetic Biology

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pREXNH3

    Plasmid

    #47079

    Purpose

    FX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and N-terminal 10x His tag and 3C protease cleavage site

    Depositor

    Eric Geertsma, Raimund Dutzler

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Lactococcus lactis expression

    Tags

    3C protease site (PreScission site) and decaHis (…

    Promoter

    NIS (nisin)

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGBW-m4046299

    Plasmid

    #153878

    Purpose

    Yeast expression plasmid for SARS-CoV-2 S (surface glycoprotein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available Since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pJA54

    Plasmid

    #60212

    Purpose

    cleavage near e1350 locus in sqt-1

    Depositor

    Andrew Fire

    Article

    Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

    Insert

    sqt-1(e1350) gRNA1
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GLYR1

    Plasmid

    #38946

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    GLYR1 (GLYR1 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

20. library backbone 3'LTR gRNA and barcode

    Plasmid

    #127397

    Purpose

    Digestion by BstXI and BamHI to release the backbone

    Depositor

    Xiaowei Zhuang

    Article

    Wang et al Proc Natl Acad Sci U S A. 2019 May 28;11

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Puro-mCherry

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGBW-m4134207

    Plasmid

    #152431

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;3xFlag

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    July 30, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pMTB-Multibow-hB

    Plasmid

    #60992

    Purpose

    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear EBFP2.

    Depositor

    Sean Megason

    Article

    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

    Insert

    EBFP2
    

    Use

    Zebrafish

    Tags

    histone 2B tag

    Promoter

    Bactin2

    Available Since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pcDXNSM3

    Plasmid

    #49028

    Purpose

    FX cloning mammalian cell line expression vector with CMV promoter and N-terminal streptavidin binding peptide, Myc tag and 3C protease cleavage site

    Depositor

    Eric Geertsma, Raimund Dutzler

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Tags

    3C protease site (PreScission site), MYC, and str…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGBW-m4133141

    Plasmid

    #152404

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;3xFlag

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    June 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pJA55

    Plasmid

    #59935

    Purpose

    gRNA for cleavage near sqt-1(e1350) locus in C elegans

    Depositor

    Andrew Fire

    Article

    Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

    Insert

    sqt-1(e1350) gRNA2
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGBW-m4046457

    Plasmid

    #145424

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pGBW-m4046390

    Plasmid

    #148996

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available Since

    May 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. 4YZ8

    Plasmid

    #69955

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    WHSC1L1:JMC055-F10:C223308 (NSD3 Human)
    

    Tags

    His and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO

    Available Since

    Oct. 7, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

29. pSaI14

    Plasmid

    #8614

    Depositor

    Robert Hartley

    Article

    Krajcikova et al J Bacteriol 1998 Mar;180(6):1582-5.

    Insert

    Ribonuclease inhibitor SaI14
    

    Expression

    Bacterial

    Mutation

    N terminal threonine changed to alanine

    Available Since

    Jan. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

30. pGBW-m4046207

    Plasmid

    #145559

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp6

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp6 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pGBW-m4046418

    Plasmid

    #149029

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pGBW-m4046476

    Plasmid

    #145512

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 E (envelope) (E Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pMTB-Multibow-hY

    Plasmid

    #60995

    Purpose

    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear citrine

    Depositor

    Sean Megason

    Article

    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

    Insert

    citrine
    

    Use

    Zebrafish

    Tags

    histone 2B tag

    Promoter

    Bactin2

    Available Since

    Feb. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGBW-m4046475

    Plasmid

    #145531

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pCY 3010-05

    Plasmid

    #36211

    Depositor

    Anne Robinson

    Article

    Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

    Insert

    LEU2
    

    Use

    Yeast cassette for pcr and homologous recombinati…

    Tags

    yEpolylinker-Cerulean-LEU2

    Available Since

    July 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGBW-m4046426

    Plasmid

    #149140

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF7b

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF7b (ORF7b Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. pGBW-m4134288

    Plasmid

    #152098

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHis-003

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    May 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pGBW-m4046406

    Plasmid

    #153880

    Purpose

    Yeast expression plasmid for SARS-CoV-2 S (surface glycoprotein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available Since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. pGBW-m4133930

    Plasmid

    #152574

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHis-003

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    June 18, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. 5C7M (ITCH)

    Plasmid

    #69966

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ITCH:SDC094-D06:C29524 (ITCH Human)
    

    Tags

    His and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO

    Available Since

    Oct. 7, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

41. pST50Trc2-DHFRHIS

    Plasmid

    #63967

    Purpose

    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pGBW-m4046339

    Plasmid

    #145565

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp8

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp8 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

43. pGBW-m4046441

    Plasmid

    #145556

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp2

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp2 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

44. pGBW-m4046165

    Plasmid

    #145540

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp7

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp7 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

45. pGBW-m4046448

    Plasmid

    #145426

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF7b

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF7b (ORF7b Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

46. pGBW-m4134112

    Plasmid

    #152084

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;V5

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    May 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

47. pLIC.B3.AF2373

    Plasmid

    #11529

    Depositor

    Sung-Hou Kim

    Article

    Liu et al J Mol Biol. 2005 Nov 25. 354(2):289-303.

    Insert

    NAD kinase (AF2373 A. fulgidus)
    

    Tags

    HisTag, TEV cleavable

    Expression

    Bacterial

    Available Since

    April 10, 2006

    Availability

    Academic Institutions and Nonprofits only

48. pMTB-Multibow-hR

    Plasmid

    #60997

    Purpose

    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear dTomato

    Depositor

    Sean Megason

    Article

    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

    Insert

    dTomato
    

    Use

    Zebrafish

    Tags

    histone 2B tag

    Promoter

    Bactin2

    Available Since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

49. pGBW-m4046219

    Plasmid

    #153926

    Purpose

    Yeast expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available Since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

50. pGBW-m4134323

    Plasmid

    #152878

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;TapTag

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    July 30, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge