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We narrowed to 54,614 results for: tra

Showing: 4221 - 4240 of 54614 results

  2. AP2u2-CMV-Ace2-IgG1

    Plasmid
    #226455
    Purpose
    Mammalian expression of human Ace2 protein under CMV promoter fused with IgG1, tagged with 6His, and released extracellular environment after expression for protein purification
    Depositor
    Urartu Seker
    Article
    Ozcelik et al ACS Pharmacol Transl Sci. 2024 Mar 21;7(
    Inserts
    Angiotensin Converting Enzyme 2
    Immunoglobulin Heavy Constant Gamma 1
    Use
    Tags
    6xHis Tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    226455-plasmid-map-sequence-id-458394

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  4. pSN-A112C-1A32

    Plasmid
    #191241
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Use
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available since
    Feb. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    191241-plasmid-map-sequence-id-384667

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  5. pSN-A112C-1A31

    Plasmid
    #191242
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Use
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    191242-plasmid-map-sequence-id-382360

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  6. pSN-A112C-CP8B1

    Plasmid
    #191240
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Use
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    191240-plasmid-map-sequence-id-380472

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  7. sgRNA targeting human SLC12A5 gene stop codon

    Plasmid
    #158577
    Purpose
    For generate double-strand DNA break near the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.
    Depositor
    Rudolf Jaenisch
    Article
    Tang et al Sci Transl Med. 2019 Jul 31;11(503). pii
    Insert
    sgRNA targeting human SLC12A5 gene stop codon
    Use
    CRISPR
    Tags
    Cas9 and GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    158577-plasmid-map-sequence-id-311925

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  11. pCDNA3.3_CoV2_B.1.1.7

    Plasmid
    #170451
    Purpose
    Express SARS-CoV-2 B.1.1.7 (UK strain / alpha strain) spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 (UK strain) pseudotyped virus production.
    Depositor
    David Nemazee
    Article
    Yuan et al Science. 2021 Aug 13;373(6556):818-823.
    Insert
    SARS-CoV-2 501V2 spike D18 (S SARS-CoV-2, UK strain (B.1.1.7))
    Use
    Tags
    NA
    Expression
    Mammalian
    Mutation
    HY69-70del, Y144del, N501Y, A570D, D614G, P681H, …
    Promoter
    CMV
    Available since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    170451-plasmid-map-sequence-id-333038

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  16. Venus2-Y705F/S727A-STAT3

    Plasmid
    #123177
    Purpose
    Expresses Y705F/S727A STAT3 double mutant fused to the 158-238 fragment of Venus in mammalian cells
    Depositor
    Federico Herrera
    Article
    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.
    Insert
    STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
    Use
    Tags
    Venus2 (aa 158-238) for bimolecular fluorescence …
    Expression
    Mammalian
    Mutation
    Double Y705F and S727A substitutions
    Promoter
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123177-plasmid-map-sequence-id-238660

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  18. Venus1-Y705F/S727A-STAT3

    Plasmid
    #123176
    Purpose
    Expresses Y705F/S727A STAT3 double mutant fused to the 1-157 fragment of Venus in mammalian cells
    Depositor
    Federico Herrera
    Article
    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.
    Insert
    STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
    Use
    Tags
    Venus1 (aa 1-158) for bimolecular fluorescence co…
    Expression
    Mammalian
    Mutation
    Double Y705F and S727A substitutions
    Promoter
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123176-plasmid-map-sequence-id-238648

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Showing: 4221 - 4240 of 54614 results