We narrowed to 17,417 results for: She
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Plasmid#229029PurposeExpresses ePE6d — engineered SpCas9 nickase (nCas9), RNA chaperone nucleocapsid (NC) protein, with T128N/N200C/V223Y variant of MMLVΔRNaseH — for gene editing in riceDepositorInsertCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-FOXM1-shRNA-81
Plasmid#89497PurposeLentiviral vector with dox inducible expression of shRNA targeting human FOXM1.DepositorInsertFOXM1 (FOXM1 Human)
UseLentiviral and RNAi; Doxycycline inducibleTagsExpressionMammalianMutationPromoterH1/TOAvailable sinceJune 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
TCF4 in pENTR
Plasmid#216569Purposegateway cloning donor vector containing TCF4DepositorInsertTCF4 (TCF4 Human)
UseGateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-FOXM1-shRNA-#2
Plasmid#89499PurposeLentiviral vector with dox inducible expression of shRNA targeting human FOXM1.DepositorInsertFOXM1 (FOXM1 Human)
UseLentiviral and RNAi; Doxycycline inducibleTagsExpressionMammalianMutationPromoterH1/TOAvailable sinceJune 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCW-GFP-CtIP
Plasmid#71109Purposemammalian expession of GFP-CtlPDepositorInsertCtlP
UseLentiviralTagsGFPExpressionMammalianMutationPromoterTRE promoter, Tet ONAvailable sinceDec. 9, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
CMV-Citroff1
Plasmid#134304PurposeMammalian expression of inverse-response citrate sensorDepositorInsertCitroff1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTW211b5
Plasmid#136927PurposeDirected evolution of SpCas9DepositorInsertCas9(N)-NpuN
UseCRISPRTagsExpressionBacterialMutationsee manuscriptPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-mito-CitronRH
Plasmid#158547PurposeMitochondria-targeted nonbinding variant of direct-response citrate sensorDepositorInsertCitronRH
UseTagsExpressionMammalianMutationPromotercmvAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lamin A (R377H)-mRFP
Plasmid#124270PurposeLamin A mutant (R377H), C-terminally tagged with mRFP. Mutation associated with EDMD myopathy.DepositorInsertLMNA (R377H) (LMNA Human)
UseTagsmRFPExpressionMammalianMutationLMNA (R377H)PromoterAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
OA-986B
Plasmid#124999PurposeExpresses dCas9-VPR driven by the Ubiquitin-63E promoterDepositorInsertdCas9-VPR
UsePiggybackTagsExpressionInsectMutationPromoterUbiquitinAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAJE-Ma pylRS nitroY/haloY-F5
Plasmid#225684PurposeExpresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control during Ma Pyl tRNA-synthetase validation.DepositorInsertsM. alvus PylRS 3-NY/HaloY F5
M. alvus Pyl-tRNA(6)
UseMethanomethylophilus alvusTagsExpressionMutationCTG125CTT, N166A, V168S, A223C, W239RPromoterGlnS and LppAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
2xMARS
Plasmid#205233PurposeExpresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-iin mammalian cellsDepositorInserttwo repeats of PLEKHA5 aa 143-271 with K163A and R164A mutations (PLEKHA5 Human)
UseTagsNuclear Export Sequence and mScarlet-iExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…PromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-FOXM1-shRNA-84
Plasmid#89496PurposeLentiviral vector with dox inducible expression of shRNA targeting human FOXM1.DepositorInsertFOXM1 (FOXM1 Human)
UseLentiviral and RNAi; Doxycycline inducibleTagsExpressionMammalianMutationPromoterH1/TOAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCLIPf-Cox8A Control Plasmid
Plasmid#101130PurposeExpression of CLIP-Tagged Cox8A in Mammalian cellsDepositorInsertCox8A (COX8A Human)
UseTagsCLIP-tag (CLIPf)ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-CaMKIIa-HA-hM3D(Gq)-IRES-mCitrine
Plasmid#50466PurposeGq-coupled hM3D-IRES-mCitrine under the control of CaMKIIa promoterDepositorInserthM3D(Gq)-IRES-mCitrine (CHRM3 Human)
UseAAVTagsHAExpressionMutationSee supplemental documents for DREADD mutationsPromoterCaMKIIaAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPHT-PKA
Plasmid#60936PurposeFluorescent FPX sensor for cAMPDepositorInsertPKA FPX Sensor
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCherry-BAF
Plasmid#172447PurposeExpression of nuclear protein BAF with an N-terminal mCherry tagDepositorInsertBAF
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDM_T7_HisLacZomega
Plasmid#118815PurposeT7 RNAP-driven expression of N-terminal His-tagged lacZ omega subunit.DepositorInsertlacZ_
UseTagshistagExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-GCaMP6s
Plasmid#105714PurposeCan be used to express GCaMP6s in the presence of Flp recombinase. Can be used to create adeno-associated virus to express Flp-dependent GCaMP6s.DepositorHas ServiceAAV8InsertGCaMP6s
UseAAV; Flp/frtTagsExpressionMammalianMutationPromoterEf1a, Human elongation factor-1 alphaAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBF3038
Plasmid#26850DepositorInsertCreA recombinase
UseCre/LoxTagsExpressionYeastMutationN/APromoterAvailable sinceDec. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
p5E-2.3k-fabp2
Plasmid#84927Purpose–2.3kb promoter of zebrafish fabp2/ifabp gene in Gateway 5’ Entry vectorDepositorInsert–2.3kb promoter of zebrafish fabp2/ifabp gene (fabp2 Zebrafish)
UseTagsNOExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-FOXM1-shRNA-#3
Plasmid#89500PurposeLentiviral vector with dox inducible expression of shRNA targeting human FOXM1.DepositorInsertFOXM1 (FOXM1 Human)
UseLentiviral and RNAi; Doxycycline inducibleTagsExpressionMammalianMutationPromoterH1/TOAvailable sinceJune 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pALS3-Ma-sfGFP-TAG150
Plasmid#212121PurposeFluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-TAG150 and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.DepositorInsertssfGFP-150TAG
M. alvus Pyl-tRNA(6)
UseTagsHis6ExpressionBacterialMutationN150TAGPromoteraraC and lppAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK-Ma pylRS nitroY/haloY-F5
Plasmid#212125PurposeExpresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control for Ma Pyl tRNA-synthetase selections.DepositorInsertM. alvus PylRS 3-NY/HaloY F5
UseTagsExpressionBacterialMutationCTG125CTT, N166A V168S, A223C, W239RPromoterGlnS (constitutive)Available sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 HA FoxO1
Plasmid#14936DepositorInsertFoxO1 (Foxo1 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
FKBP-eGFP-TAZ
Plasmid#221213Purposepart A of TAZ-SPARK-OFFDepositorInsertFKBP-eGFP-TAZ
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
DL015:CMV::iPAK4
Plasmid#177880Purposeibox-PAK4cat (iPAK4) fusion with CMV promoter, cloned into a lentivirus backboneDepositorInsertiPAK4
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
PRDX6A-c001
Plasmid#175474PurposeProtein expression in bacterial cells. Entire CDS, M1-P224. Can be biotinylated.DepositorInsertPRDX6 (PRDX6 Human)
UseTagsAviTag and His6-TEVExpressionBacterialMutationSee Depositor CommentsPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6-Universal
Plasmid#52694PurposeThis plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.DepositorInsertsCas9
Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
UseCRISPR; Toxoplasma gondiiTags3X FLAG and Nuclear localization signalExpressionMutationPromoterTgTUB1 and Toxoplasma U6Available sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
DL016:CMV::eGFP-iPAK4
Plasmid#177881PurposeeGFP-IPAK4 fusion with CMV promoter, cloned into a lentivirus backboneDepositorInsertiPAK4
UseLentiviralTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GluA2-scFv-mNeon-KDEL
Plasmid#230057PurposeAAV plasmid that expresses a a single chain variable fragment (scFv) for anti- GluA2 with a C-terminal mNeon. Encodes an ER retrieval motif (KDEL) at C-terminus of mNeon.DepositorInsertAnti-GluA2 single chain variable fragment (scFv)
UseAAVTagsER retrieval motif (KDEL) and mNeonExpressionMammalianMutationPromoterhuman synapsinAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
p5'Entry-lck-7.4kb
Plasmid#58891Purposeprovides zebrafish lck promoter (7.4 kb) for GateWay construction of Tol2 transgenesDepositorInsertzebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 7.4 kb
UseGateway 5' entry cloneTagsExpressionMutationmutated single nucleotide (T-->C, 1874th nucle…Promoterlymphocyte-specific protein tyrosine kinase (lck)…Available sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETNb-cALFA
Plasmid#159989PurposeFor the protein expression with ALFA tag at C-terminus in E. coliDepositorTypeEmpty backboneUseTagsHRV3C protease cleavage site-ALFA tag-8histidineExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
DL017:CMV::HaloTag-iPAK4
Plasmid#177882PurposeHT-iPAK4 fusion with CMV promoter, cloned into a lentivirus backboneDepositorInsertiPAK4
UseLentiviralTagsHaloTagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
HECTD1
Plasmid#32860PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHECTD1 (HECTD1 Human)
UseTagsHis (with TEV cleavage)ExpressionBacterialMutationamino acids 1879-1966 onlyPromoterAvailable sinceJan. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBac_Dual_SNF2L-opt_p48
Plasmid#102243PurposeExpression of human codon optimized human SNF2L and pRbAp48 from pFastBac dualDepositorUseTagsExpressionInsectMutationcodon optimized for expressionPromoterPolyhedrin and p10Available sinceNov. 1, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
AICSDP-35:AAVS1-mEGFP
Plasmid#91565PurposeHomology arms and linker-mEGFP sequence for internal insertion of CAGGS-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFPDepositorInsertAAVS1 Homology Arms with CAGGS driven mEGFP (AAVS1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 7, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine
Plasmid#50472PurposeGs-coupled rM3D-IRES-mCitrine under the control of GFAP promoterDepositorUseAAVTagsHAExpressionMutationSee supplemental documents for DREADD mutationsPromoterGFAPAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
epB_UbC_CymRV5-nls-Halox2_DEx4
Plasmid#119907PurposeePiggyBac mammalian expression plasmid for CymR-Halo fusionDepositorInsertCymRV5-nls-Halox2
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2019AvailabilityAcademic Institutions and Nonprofits only