163,421 results

1. pLenti-Cas9-GFP

   Plasmid

   #86145

   Purpose

   Lentiviral vector expressing Cas9-GFP

   Depositor

   David Sabatini

   Article

   Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

   Insert

   Cas9-GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-Mm.cargo(Cas9)

   Plasmid

   #174865

   Purpose

   Expresses mouse Peg10 cargoRNA encoding SpCas9

   Depositor

   Feng Zhang

   Article

   Segel et al Science. 2021 Aug 20;373(6557):882-889.

   Insert

   SpCas9 flanked by MmPeg10 UTRs
   

   Use

   Tags

   HA-SV40 NLS and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBabe K-Ras 12V

   Plasmid

   #12544

   Depositor

   Channing Der

   Article

   Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

   Insert

   K-Ras 12V (KRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   12V constituitively activated

   Promoter

   Available since

   Sept. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUltra-Plus-H2B-EGFP

   Plasmid

   #161776

   Purpose

   3rd generation lentiviral bicistronic vector for mammalian expression of H2B-EGFP and one gene of interest

   Depositor

   Jorge Luis Arias-Arias

   Insert

   H2B-EGFP from Addgene Plasmid #26790
   

   Use

   Lentiviral; Bicistronic

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   UbC and UbC promoter

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLX208 CMV sTurboID (C)-HA-FRB-ERM

   Plasmid

   #153007

   Purpose

   Expresses split-TurboID C-terminal fragment targeted to endoplasmic reticulum membrane

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   sTurboID (C) (G74)-ERM (Cb5)
   

   Use

   Lentiviral

   Tags

   FRB and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. GFP-SMN

   Plasmid

   #37057

   Depositor

   Greg Matera

   Article

   Shpargel et al Proc Natl Acad Sci U S A. 2005 Nov 29;10

   Insert

   Human SMN1 (SMN1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MG1655 ΔendA ΔrecA (DE3)

   Bacterial Strain

   #37854

   Depositor

   Kristala Prather

   Article

   Tseng et al Microb Cell Fact. 2010 Nov 27;9:96.

   Bacterial Resistance

   None

   Available since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

8. Human Base Editing Sensor Library

   Pooled Library

   #179218

   Purpose

   sgRNAs that target frequently occurring cancer associated mutations identified in the MSK IMPACT dataset that can be modeled with cytosine base editing.

   Depositor

   Lukas Dow

   Article

   Sanchez-Rivera et al Nat Biotechnol. 2022 Feb 14. pii: 10.103

   Expression

   Mammalian

   Use

   Lentiviral

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

9. pCG150_double_phiC31_attB

   Plasmid

   #169693

   Purpose

   cargo vector for the phiC31 system with, unc-119 rescue fragment, no fluorescent marker. Gateway R3R4 sites (for gene of interest insertion)

   Depositor

   Shih-Peng Chan

   Article

   Yang et al Genetics. 2022 Feb 4;220(2):iyab206. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGTag-NLS-eGFP-SV40

    Plasmid

    #117811

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pX459V2.0-HypaCas9

    Plasmid

    #108294

    Purpose

    pX459 V2.0 (Plasmid #62988) with the N692A, M694A, Q695A, and H698A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CROPseq-multi-Puro (pRW1441)

    Plasmid

    #216217

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi construct with Puromycin resistance

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDH-EF1-Large BiT - SNAP-tag - Beta-actin

    Plasmid

    #171019

    Purpose

    Lentiviral expression of a Large BiT-SNAP-tag-Beta-actin fusion in mammalian cells

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    LargeBit-SNAPtag- Beta-actin
    

    Use

    Lentiviral and Luciferase

    Tags

    Beta actin and LargeBiT

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-Cyclin D1

    Plasmid

    #19927

    Depositor

    Yue Xiong

    Article

    Watanabe et al Proc Natl Acad Sci U S A. 1998 Feb 17. 9

    Insert

    Cyclin D1 (CCND1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG-eCas9-GFP-U6-gRNA

    Plasmid

    #79145

    Purpose

    All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in human pluripotent stem cells

    Depositor

    Jizhong Zou

    Insert

    eSpCas9(1.1)
    

    Use

    CRISPR

    Tags

    2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3 FLAG FBXW7alpha

    Plasmid

    #236470

    Purpose

    transient overexpression of FBXW7alpha in mammalian cells

    Depositor

    Michele Pagano

    Article

    Busino et al Nat Cell Biol. 2012 Mar 4;14(4):375-85.

    Insert

    FBXW7alpha (FBXW7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PGKp-GFP-Empty

    Plasmid

    #174175

    Purpose

    Control lentiviral vector expressing GFP under control of the human PGK promoter.

    Depositor

    Rod Bremner

    Article

    Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Aug. 19, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. mEmerald-Claudin7-C-12

    Plasmid

    #54041

    Purpose

    Localization: Tight Junctions, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    Claudin7 (CLDN7 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pET28a_T7-ARG1

    Plasmid

    #106473

    Purpose

    Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli

    Depositor

    Mikhail Shapiro

    Article

    Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

    Insert

    ARG1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR221_SLC1A2

    Plasmid

    #131901

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC1A2 (SLC1A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pGR595

    Plasmid

    #213785

    Purpose

    Integrates at the CAN1 locus the BadBoy2 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette.

    Depositor

    Chang Liu

    Insert

    BadBoy2 TP-DNAP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCF525-mTagBFP2_EF1a-Hygro-P2A-mTagBFP2_lenti

    Plasmid

    #138315

    Purpose

    Lenti expression of mTgBFP2

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    mTAGBFP2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. GFP-Epac2

    Plasmid

    #118314

    Purpose

    Expression of mouse Epac2

    Depositor

    Philip Stork

    Article

    Liu et al Mol Cell Biol. 2008 Dec;28(23):7109-25.

    Insert

    Epac2 (Rapgef4 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJT260 Lenti pEF-dCas9-3XFLAG-VPR-T2A-tagBFP-P2A-Blast

    Plasmid

    #187336

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-VPR-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. XLone-Puro eGFP

    Plasmid

    #140027

    Purpose

    Tunable and temporal expression control of nuclear eGFP

    Depositor

    Xiaoping Bao

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1-hACE2

    Plasmid

    #145033

    Purpose

    Express human ACE2 with C9 tag at C-terminal in mammalian cells

    Depositor

    Fang Li

    Article

    Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

    Insert

    human ACE2 (ACE2 Human)
    

    Use

    Tags

    C9

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. M13KO7-ΔPS-ΔgeneIII

    Plasmid

    #134351

    Purpose

    Helper phage for M13 phage production

    Depositor

    Mark Isalan

    Article

    Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

    Insert

    Helper phage gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

    Plasmid

    #60231

    Purpose

    Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Cre recombinase
    EGFP
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA-P2A and EGFP-KASH

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and hSyn

    Available since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. p4455 FLAG-hPLIC-2

    Plasmid

    #8661

    Depositor

    Peter Howley

    Article

    Kleijnen et al Mol Cell 2000 Aug;6(2):409-19.

    Insert

    hPLIC-2 (UBQLN2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEGFP-C1-AR

    Plasmid

    #28235

    Purpose

    Mammalian expression of Androgen receptor fused to EGFP

    Depositor

    Michael Mancini

    Article

    Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

    Insert

    Androgen receptor (AR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge