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Showing: 6391 - 6420 of 160465 results

  10. pCAT009

    Plasmid
    #171635
    Purpose
    Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting B2M and CAG promoter expressing mTagBFP2
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-B2M
    mTagBFP2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171635-plasmid-map-sequence-id-336490

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  14. pCAT100

    Plasmid
    #180512
    Purpose
    Plasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv2
    PlmCasX with DpbCasX R1 loop
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180512-plasmid-map-sequence-id-359730

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  18. GPR116-Tango

    Plasmid
    #66307
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    GPR116 (ADGRF5 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  20. pET28-iLOVf

    Plasmid
    #63723
    Purpose
    E coli expression plasmid to produce Thrombin cleavable His tagged iLOV fusion proteins
    Depositor
    Brian Smith
    Type
    Empty backbone
    Use
    Tags
    TEE, iLOV, 6xHis, thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    63723-plasmid-map-sequence-id-110780

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  29. pCW57.1_V5_TurboID_FLAG_Ct

    Plasmid
    #194073
    Purpose
    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    FLAG-TurboID-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    194073-plasmid-map-sequence-id-380729

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Showing: 6391 - 6420 of 160465 results