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173,176 results

Showing: 6511 - 6540 of 173176 results

  1. pGG-NES-TurboID-GFP

    Plasmid
    #209401
    Purpose
    Transiently expressing and visualizing the subcellular localization of TurboID fused with a nuclear export signal (NES) in planta
    Depositor
    Rosa Lozano-Durán
    Article
    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1
    Insert
    NES-3XHA-TurboID
    Tags
    GFP
    Expression
    Plant
    Mutation
    TurboID gene sequence C249A
    Promoter
    Cauliflower mosaic virus (CaMV) 35S promoter
    Available Since
    Jan. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    209401-plasmid-map-sequence-id-418454

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  2. pLSB-KAN

    Plasmid
    #166700
    Purpose
    Combined sgRNA/Cas9 pLSB vector with kanMX6 (G418) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Article
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Expression
    Yeast
    Promoter
    adh15 promoter and tRNA Ser promoter
    Available Since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    166700-plasmid-map-sequence-id-329224

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  5. pYPQ230-RRVQ

    Plasmid
    #200188
    Purpose
    Gateway entry clone (attL1 & attR5) for LbCas12a-RRVQ mediated genome editing
    Depositor
    Yiping Qi
    Article
    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:
    Insert
    LbCas12a-RRVQ
    Use
    CRISPR; Gateway compatible lbcas12a-rrv entry clo…
    Tags
    NLS
    Expression
    Plant
    Mutation
    G146R, D156R, R182V, E795Q
    Available Since
    June 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    200188-plasmid-map-sequence-id-396764

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  6. pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine (AAV8)

    Viral Prep
    #50454-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine (#50454). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine plasmid DNA. Synapsin-driven, Cre-dependent, excitatory hM3D DREADD expression, with physically separate mCitrine expression for cell body labeling. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    mCitrine (Cre-dependent)
    Available Since
    Sept. 20, 2018
    Availability
    Academic Institutions and Nonprofits only

  7. TFORF1275

    Plasmid
    #143781
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TPRX1 (TPRX1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143781-plasmid-map-sequence-id-391569

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  15. Go1-CASE

    Plasmid
    #168123
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAO1, GNB3, GNG9 (GNAO1, GNB3, GNGT2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG9
    Expression
    Mammalian
    Mutation
    NLuc is inserted at L91/G92 within GNAO1
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168123-plasmid-map-sequence-id-332865

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  20. AAV-EF1a-BbTagBY (AAV9)

    Viral Prep
    #45185-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from AAV-EF1a-BbTagBY (#45185). In addition to the viral particles, you will also receive purified AAV-EF1a-BbTagBY plasmid DNA. Brainbow construct encoding farnesylated TagBFP and EYFP, both in reverse orientation between mutant Lox sites. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Dawen Cai, Joshua Sanes
    Article
    Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi
    Promoter
    EF1a
    Tags
    TagBFP, EYFP, or none.
    Available Since
    March 7, 2018
    Availability
    Academic Institutions and Nonprofits only

  21. Nluc CD63

    Plasmid
    #242528
    Purpose
    This plasmid can be used to quantify CD63 by luminescence signal upon providing substrate, in particular in the secretome, especially in the extracellular vesicles of cells expressing this construct.
    Depositor
    Clotilde Thery
    Article
    Grisard et al J Extracell Vesicles. 2022 Jul;11(7):e12
    Insert
    CD63 (CD63 Human)
    Use
    Luciferase
    Tags
    Nanoluciferase
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    242528-plasmid-map-sequence-id-500759

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  22. pAAV-hSyn-EGFP (AAV9 trial size)

    Viral Prep
    #50465-AAV9.T
    Purpose
    Ready-to-use AAV9 trial size particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    EGFP
    Available Since
    May 29, 2019
    Availability
    Academic Institutions and Nonprofits only

  23. pD649-HAsp-ISLR2_Ig1-3-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #157619
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    ISLR2_Ig1-3 (ISLR2 Human)
    Tags
    Fc(DAPA)-Avi-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    Jan. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    157619-plasmid-map-sequence-id-382008

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  25. pEMS1600

    Plasmid
    #29274
    Purpose
    Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PN
    Depositor
    Elizabeth Simpson
    Article
    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1
    Insert
    Ple129 (MKI67 Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    Intron-LacZ-NLS
    Available Since
    Nov. 4, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
    29274-plasmid-map-sequence-id-13689

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  26. Cas9 CFP

    Plasmid
    #155278
    Purpose
    Lentiviral Cas9 expression with CFP marker
    Depositor
    Martin Carroll
    Insert
    spCas9
    Expression
    Mammalian
    Available Since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    155278-plasmid-map-sequence-id-309981

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  28. PB-eDIO-ChR2-EYFP

    Plasmid
    #104387
    Purpose
    PiggyBac plasmid encoding channelrhodopsin 2 fused to EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.
    Depositor
    Volker Busskamp
    Article
    Klapper et al Sci Rep. 2017 Oct 31;7(1):14450. doi: 10
    Insert
    Channelrhodopsin 2
    Use
    Cre/Lox; Piggybac
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    E134R
    Available Since
    Feb. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    104387-plasmid-map-sequence-id-203589

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  29. DT034A

    Plasmid
    #159395
    Purpose
    Constitutive LuxR expression, controlled under E.coli hybrid (J23117-J23106) promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoter
    Depositor
    Sheref Mansy
    Article
    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea
    Insert
    pr.J23117/J23106-LuxR-pr.LuxR-PFO-pdt
    Use
    Synthetic Biology
    Tags
    FLAG/FLAG and pdt#3 from Collins Nat. Biotech. 20…
    Expression
    Bacterial
    Promoter
    J23117 and pLux promoter
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    159395-plasmid-map-sequence-id-393061

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Showing: 6511 - 6540 of 173176 results