167,846 results

1. pcDNA HA HP1 gamma

   Plasmid

   #24080

   Depositor

   Naoko Tanese

   Article

   Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

   Insert

   HP1 gamma (CBX3 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Oct. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-Flag-Kdm4b-pA

   Plasmid

   #104486

   Purpose

   Express mouse Kdm4b in mammalian cells. It has a T7 promoter for in vitro transcription. Since polyA is encoded on the vector, polyA-tailing is unnecessary.

   Depositor

   Yi Zhang

   Article

   Inoue et al Genes Dev. 2017 Oct 1;31(19):1927-1932.

   Insert

   Kdm4b (Kdm4b Mouse)
   

   Tags

   Flag epitope tag

   Expression

   Mammalian

   Available Since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEMS-II-PEST

   Plasmid

   #204766

   Purpose

   GEMS 2.0 sensor system with PEST destabilization domain (pCMV-EGFP-PEST-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577+PEST)

   Depositor

   Kate Meyer

   Article

   Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   EGFP-PEST-m6Alinker-DHFR
   

   Mutation

   mutated all RACs in EGFP to RGC/RCC

   Promoter

   CMV

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. YFP-Parkin

   Plasmid

   #23955

   Purpose

   Mammalian expression of human Park2 fused to EYFP

   Depositor

   Richard Youle

   Article

   Narendra et al J Cell Biol. 2008 Dec 1. 183(5):795-803.

   Insert

   Park2 (PRKN Human)
   

   Tags

   eYFP

   Expression

   Mammalian

   Available Since

   April 9, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(-) MID51 4xMyc Hisx6

   Plasmid

   #44598

   Depositor

   David Chan

   Article

   Loson et al Mol Biol Cell. 2013 Jan 2.

   Insert

   MiD51 (Mief1 Mouse)
   

   Tags

   4xMyc and 6xHis

   Expression

   Mammalian

   Available Since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTF-ahpC-rfp

   Plasmid

   #153035

   Purpose

   For CRISPR-Cas12a genome editing in E. coli. Contains a constituitively expressed CRISPR array targeting the ahpC gene in E. coli and donor DNA to insert an rfp translational fusion in the ahpC gene.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   CRISPR array
   

   Expression

   Bacterial

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Anti-Neurexin-1-Beta [N170A/1.1R] (Recombinant Antibody)

   Antibody

   #180100-rAb

   Purpose

   Anti-Neurexin-1-Beta (Human) recombinant mouse monoclonal antibody

   Depositor

   James Trimmer

   Article

   Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

   Recommended Applications

   Immunocytochemistry

   Reactivity

   Human, Mouse, and Rat

   Source Species

   Mouse

   Isotype

   IgG2a

   Trial Size

   Available to purchase

   Available Since

   June 15, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

8. pLenti-PGK-Venus-Akaluc (neo)

   Plasmid

   #124701

   Purpose

   Aka-Luciferase reporter

   Depositor

   Roland Friedel

   Article

   Bozec et al Neurooncol Adv. 2020 Oct 10;2(1):vdaa134

   Insert

   Venus-Akaluciferase
   

   Use

   Lentiviral

   Promoter

   hPGK

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSW031

   Plasmid

   #231144

   Purpose

   Used to express cyclic sortase cW11 (recognizes APXTG motif) from E. coli.

   Depositor

   Philip Cole

   Article

   Whedon et al J Am Chem Soc. 2024 Nov 25. doi: 10.1021

   Insert

   S. aureus Sortase A mutant
   

   Tags

   Nostoc punctiform split intein C-terminal fragmen…

   Expression

   Bacterial

   Mutation

   P94S, E108Q, S116V, D160N, T164Q, V168M, L169H, D…

   Promoter

   T7

   Available Since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNIC-HIS6-MBP-TEV-GG

    Plasmid

    #215800

    Purpose

    Cloning of target gene with N terminal His6 MBP tag and TEV Cleavage

    Depositor

    Frank von Delft, ASAP Discovery

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. Luc-PS9

    Plasmid

    #177942

    Purpose

    Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    Luc2 (ORF1ab )
    

    Expression

    Mammalian

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDawn

    Plasmid

    #43796

    Purpose

    Empty backbone for blue-light-regulated expression of a gene of interest. Expression is induced under blue illumination.

    Depositor

    Andreas Moeglich

    Article

    Ohlendorf et al J Mol Biol. 2012 Mar 2;416(4):534-42. do

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGfa2-nLac

    Plasmid

    #53126

    Purpose

    Expression of LacZ driven by the human Gfa2 promoter. LacZ can also be replaced with the gene of interest for targeting transgene expression to astrocytes in mice.

    Depositor

    Michael Brenner

    Article

    Brenner et al J Neurosci. 1994 Mar;14(3 Pt 1):1030-7.

    Insert

    Gfa2 (GFAP Human)
    

    Use

    Mouse Targeting

    Tags

    LacZ, NLS, and mP1

    Expression

    Mammalian

    Mutation

    contains -2163 to +47 (the human gfa2 segment), w…

    Promoter

    human GFAP

    Available Since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIRE4-Bpa

    Plasmid

    #155342

    Purpose

    Plasmid encoding the translational pair that enables efficient incorporation of Benzoyl-phenylalanine in response to the amber codon into proteins in mammmalian cells.

    Depositor

    Irene Coin

    Article

    Bottke et al EMBO Rep. 2020 Sep 14:e50437. doi: 10.15

    Insert

    humanized EBpaRS/BstYam
    

    Expression

    Mammalian

    Promoter

    PGK/U6

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLENTI_TetOn_Hu_BIRC5(WT)

    Plasmid

    #136348

    Purpose

    Lentiviral inducible expression (TetOn) of human BIRC5

    Depositor

    Guillermo de Cárcer

    Insert

    BIRC5 (BIRC5 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. His6-SUMO-pET28a-EfaCas1

    Plasmid

    #112793

    Purpose

    To express enterococcus faecalis Cas1 protein in E. coli

    Depositor

    Ailong Ke

    Article

    Xiao et al Nature. 2017 Oct 5;550(7674):137-141. do

    Insert

    EfaCas1
    

    Tags

    His6-SUMO

    Expression

    Bacterial

    Available Since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. FRT LAMP3 mCRISPRed_mTagBFP2 pcDNA5

    Plasmid

    #159455

    Purpose

    Encodes a lysosome tagret fusion protein of the pH resistant proteins mCRISPRed and mTagBFP2

    Depositor

    Oliver Griesbeck

    Article

    Erdogan et al Cell Chem Biol. 2020 May 21;27(5):610-62

    Insert

    mCRISPRed
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTlpA-Wasabi

    Plasmid

    #85455

    Purpose

    mWasabi reporter gene gated by wild type TlpA

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TlpA
    mWasabi
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D341A

    Promoter

    TlpA and TlpA, LacI

    Available Since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUSE-Src-YF-UniRapR-mCerulean-myc

    Plasmid

    #45381

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

    Insert

    Src-YF-UniRapR-mCerulean-myc
    

    Tags

    Myc, Myr, and mCerulean

    Expression

    Mammalian

    Mutation

    changed mSrc Tyrosine 529 to Phenylalanine

    Promoter

    CMV

    Available Since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti6.2-ccdB-3xFLAG-V5

    Plasmid

    #87071

    Purpose

    Lentiviral Gateway-compatible expression vector backbone with a C-terminal 3xFLAG-V5 tag

    Depositor

    Mikko Taipale, Susan Lindquist

    Article

    Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3xFLAG-V5

    Expression

    Mammalian

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pBB572

    Plasmid

    #27397

    Depositor

    Bernd Bukau

    Article

    de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

    Insert

    ibpA, ibpB (ibpB, ibpA E.coli)
    

    Expression

    Bacterial

    Available Since

    March 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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22. PX459v3

    Plasmid

    #178799

    Purpose

    SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Cbh (Cas9-2A Puro) and U6 (gRNA)

    Available Since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. pFastBac1-pGC-A

    Plasmid

    #186626

    Purpose

    pFastBac1 (baculovirus polyhedrin promoter) encoding HA signal peptide, TEV-protease cleavable His10-tag, and full-length human pGC-A (NP_000897.3 amino acids 33-1061; expression-optimized DNA)

    Depositor

    Debra Hansen

    Article

    Zhang et al Sci Rep. 2022 Jul 12;12(1):11824. doi: 1

    Insert

    NPR1 (NPR1 Human)
    

    Tags

    hemagglutinin (HA) signal peptide + His10-tag + T…

    Expression

    Insect

    Mutation

    deleted amino acids 1-32

    Promoter

    polyhedring

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. tdTomato-N1

    Plasmid

    #54642

    Purpose

    Localization: N1 Cloning Vector, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson, Roger Tsien, Nathan Shaner

    Article

    Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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25. pNIC-CT-sfGFP-HN-GG

    Plasmid

    #215803

    Purpose

    Cloning of target gene with C terminal TEV cleavable sfGFP HisAsn6 tag

    Depositor

    Frank von Delft, ASAP Discovery

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pET His10 TEV LIC cloning vector (2B-T-10)

    Plasmid

    #78173

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    10xHis and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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27. MAP2K2-NanoLuc Fusion Vector

    Plasmid

    #238877

    Purpose

    Express MAP2K2-NanoLuc Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    MAP2K2 (MAP2K2 Human)
    

    Tags

    NanoLuc (R)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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28. mTagGFP2-Rab5a-7

    Plasmid

    #56417

    Purpose

    Localization: Ras GTPase, Excitation: 483, Emission: 506

    Depositor

    Michael Davidson

    Insert

    Rab5a
    

    Tags

    mTagGFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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29. SunTagng22aa

    Plasmid

    #106438

    Purpose

    Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.

    Depositor

    Steven Jacobsen

    Article

    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

    Insert

    NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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30. AAV-hSyn-PiGM-Iq(eGFP)

    Plasmid

    #221602

    Purpose

    A recombinant AAV2 plasmid encoding the PiGM-Iq system with CRY2PHR, CIBN and EGFP as expression marker. hSynapsin promoter for panneuronal expression.

    Depositor

    John Lin

    Insert

    PiGM-Iq (eGFP)
    

    Use

    AAV

    Mutation

    RGS2 1-53 truncation

    Promoter

    Human Synapsin

    Available Since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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