We narrowed to 35,144 results for: cas

1. pFnCas12a_NT

   Plasmid

   #169838

   Purpose

   Expresses FnCas12a and guide RNA in Bacteria

   Depositor

   John van der Oost

   Article

   Mohanraju et al CRISPR J. 2021 Feb;4(1):82-91. doi: 10.1

   Insert

   FnCas12a
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pXynA

   Available since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. NmCas9_sgRNA_expression_in_pBluescript

   Plasmid

   #122091

   Purpose

   U6 driven NmCas9 sgRNA expression vector for cloning own guides

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   NmCas9 tracrRNA (NEWENTRY Synthetic, S. pyogenes)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHdCas9

   Plasmid

   #137080

   Purpose

   Expression of Himar-dCas9 in bacteria

   Depositor

   Harris Wang

   Article

   Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

   Insert

   Himar-dCas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLtetO-1

   Available since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAK_DR30_CasRx_Puro

   Plasmid

   #134845

   Purpose

   All in one overexpression of CasRx and guideRNA

   Depositor

   Michael Yaffe

   Insert

   RfxCas13d
   

   Use

   CRISPR

   Tags

   T2A Puromycin

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCast

   Plasmid

   #200169

   Purpose

   E. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozyme

   Depositor

   Yong-Su Jin

   Article

   Kuanyshev et al Biotechnol Bioeng. 2021 Jan;118(1):372-3

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. M-ST1cas

   Plasmid

   #48669

   Purpose

   Mammalian S. thermophilus #1 Cas9 expression, human optimized

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAdSh.PGK.Cas9

   Plasmid

   #58253

   Purpose

   Expresses human codon-optimized S. pyogenes Cas9 from the human PGK-1 gene promoter

   Depositor

   Manuel Goncalves

   Article

   Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

   Insert

   Human codon-optimized S. pyogenes Cas9
   

   Use

   Adenoviral and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. xCas9

   Plasmid

   #138565

   Purpose

   Expresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   xCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Promoter

   EFS

   Available since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Nsp2Cas9_AAV

   Plasmid

   #192143

   Purpose

   Expresses Nsp2Cas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Wei et al Elife. 2022 Aug 12;11. pii: 77825. doi:

   Insert

   Nsp2Cas9
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCjeCas9

    Plasmid

    #172215

    Purpose

    Expresses Campylobacter jejuni Cas9 (CjeCas9) in bacterial cells

    Depositor

    John van der Oost

    Article

    Saha et al Sci Adv. 2020 Jun 17;6(25):eaaz4849. doi

    Insert

    Cas9 endonuclease from the Campylobacter jejuni Type II CRISPR/Cas system
    

    Use

    Tags

    Histag

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCasX1

    Plasmid

    #87685

    Purpose

    p15A plasmid expressing CasX locus from Deltaproteobacteria

    Depositor

    Jennifer Doudna

    Article

    Burstein et al Nature. 2016 Dec 22. doi: 10.1038/nature

    Insert

    CasX1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    None

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. G-RCAS (A)

    Plasmid

    #15182

    Depositor

    Connie Cepko

    Article

    Harpavat et al BMC Dev Biol. 2006 Jan 22;6:2. doi: 10.1

    Insert

    Gateway cassette
    

    Use

    RNAi and Retroviral; Avian expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTi4.0_SpdCas9_pmcDA1

    Plasmid

    #204613

    Purpose

    CRISPR cytosine deaminase integrative plasmid for Mycoplasma gallisepticum

    Depositor

    Pascal Sirand-Pugnet

    Article

    Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEPSA5Cas9syn2

    Plasmid

    #206963

    Purpose

    S. aureus genome editing vector removed these two motifs AGGNNNNNGAT, CCAYNNNNNNTGT, CCAYNNNNNNGTA, GGANNNNNNNNTCG, and GWAGNNNNNNGAT

    Depositor

    Daijie Chen

    Article

    Yang et al ACS Synth Biol. 2023 Oct 13. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. NAX_CAGGS_CAS9_Puro

    Plasmid

    #167863

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEPSA5Cas9syn

    Plasmid

    #206965

    Purpose

    S. aureus genome editing vector removed these two motifs AGGNNNNNGAT and CCAYNNNNNNTGT

    Depositor

    Daijie Chen

    Article

    Yang et al ACS Synth Biol. 2023 Oct 13. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDual_dsCas9_Blast_hs_NC_chr1

    Plasmid

    #214690

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)

    Depositor

    Kevin Janes

    Insert

    dgRNA_Chr1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDual_dsCas9_Blast_hs_TP73

    Plasmid

    #214691

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human TP73

    Depositor

    Kevin Janes

    Insert

    dgRNA_TP73 (TP73 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. HBT_pcoCASphi_version1

    Plasmid

    #197931

    Purpose

    Cloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminal

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Insert

    pcoCasphi-2
    

    Use

    CRISPR

    Tags

    FLAG tag and SV40 NLS and nucleoplasmin NLS

    Expression

    Plant

    Mutation

    Promoter

    35SPPDK promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pThermoCas9i_ctrl

    Plasmid

    #100982

    Purpose

    Expresses the catalytically inactive version of ThermoCas9 (Thermo(d)Cas9) and its sgRNA module

    Depositor

    John van der Oost

    Article

    Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:

    Inserts

    Catalytically inactive variant of the Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
    ThermoCas9 single guide RNA expressing module
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    changed aspartate 8 to alanine and histidine 582 …

    Promoter

    B. coagulans DSM 1 pta promoter and B. smithii xy…

    Available since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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