We narrowed to 15,100 results for: pET

1. pDONR221-TAF15 c1222t No Stop

   Plasmid

   #84900

   Purpose

   DONOR vector for Gateway cloning of TAF15 c1222t No Stop

   Depositor

   Aaron Gitler

   Article

   Couthouis et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

   Insert

   TAF15 (TAF15 Human)
   

   Use

   Gateway donor

   Tags

   Expression

   Mutation

   c1222t No Stop

   Promoter

   none

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3.3_SARS2_Mu

   Plasmid

   #177578

   Purpose

   expressing SARS-CoV-2 B.1.621 (Mu) spike protein for pseudovirus production

   Depositor

   David Nemazee

   Article

   Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

   Insert

   Spike (Mu variant) (S SARS-CoV-2)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   T95I, Y144T, Y145S, R346K, E484K, N501Y, D614G, …

   Promoter

   CMV

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.3_SARS2_omicron_BA.2.75

   Plasmid

   #190674

   Purpose

   expressing SARS-CoV-2 BA.2.75 spike protein for pseudovirus production

   Depositor

   David Nemazee

   Article

   Dacon et al Cell Host Microbe. 2022 Nov 4. pii: S193

   Insert

   Spike (S SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K147E, W152R, F157L, I210V, G257S, D339H, G446S, …

   Promoter

   CMV

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.3_SARS2_Lambda

   Plasmid

   #177579

   Purpose

   expressing SARS-CoV-2 C.37 (lambda) spike protein for pseudovirus production

   Depositor

   David Nemazee

   Article

   Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

   Insert

   Spike (Lambda variant) (S SARS-CoV-2)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   75-76VI, 246-253N, L452Q, F490S, D614G, T859N, la…

   Promoter

   CMV

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCW-eGFP-FHA-SHLD2-m1

   Plasmid

   #114122

   Purpose

   Lentiviral vector for inducible expression of N-terminally tagged eGFP-RNF8 FHA domain-SHLD2, m1 mutant; 53BP1-independent recruitment to damaged chromatin

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Insert

   SHLD2 (m1 mutant) (SHLD2 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   RNF8 FHA domain (aa 2-160) and eGFP

   Expression

   Mammalian

   Mutation

   W489A, F494A, W495A mutations introduced

   Promoter

   TRE promoter, Tet ON

   Available Since

   Aug. 28, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pSLIK-NFLAG-hEWSR1WT-CMyc

   Plasmid

   #50733

   Purpose

   Produces lentivirus expressing human EWSR1 wild type with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2G

   Depositor

   Zissimos Mourelatos

   Article

   Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

   Insert

   EWSR1 (EWSR1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and Myc

   Expression

   Mutation

   Promoter

   TRE

   Available Since

   Jan. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pSLIK-NFLAG-hEWSR1P552L-CMyc

   Plasmid

   #50736

   Purpose

   Produces lentivirus expressing human EWSR1P552L mutant with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2G

   Depositor

   Zissimos Mourelatos

   Article

   Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

   Insert

   EWSR1 P552L (EWSR1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and Myc

   Expression

   Mutation

   Changed Pro 552 to Leu

   Promoter

   TRE

   Available Since

   Jan. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pLenti-CD74-ROS1 L2000V

   Plasmid

   #183815

   Purpose

   Express CD74-ROS1 fusion (C6;R34) harboring ROS1 L2000V mutation in mammalian cells

   Depositor

   Aaron Hata

   Article

   Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

   Inserts

   ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
   CD74 molecule (CD74 Human)
   

   Use

   Lentiviral

   Tags

   The fusion protein of CD74 exon 1-6 and ROS1 exon…

   Expression

   Mammalian

   Mutation

   L2000V

   Promoter

   CMV

   Available Since

   April 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL4-eIF3B

   Plasmid

   #133308

   Purpose

   Expression of luciferase driven by eIF3B promoter region

   Depositor

   Josep Domingo-Domenech

   Article

   Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

   Insert

   eIF3B promoter (EIF3B Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   eIF3B

   Available Since

   Oct. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-hSyn-spCas9

    Plasmid

    #231404

    Purpose

    Expresses spCas9 from hSyn promoter

    Depositor

    Arjen Boender, Larry Young

    Article

    Schimmer et al bioRxiv 2024.07.15.603543; doi: https://

    Insert

    hSYN promoter (RIC8B Human)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Changed RSV promoter to hSYN promoter

    Promoter

    hSYN

    Available Since

    Oct. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. HisGB1-Fusion + CBP TAZ1(340-439)

    Plasmid

    #173762

    Purpose

    Bacterial coexpression of CBP TAZ1 and CITED2/Hif1a activation domain constructs

    Depositor

    Peter Wright

    Article

    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(

    Inserts

    CITED2(216-246):Hif1a(796-826) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    

    Use

    Tags

    His6GB1 and thrombin

    Expression

    Bacterial

    Mutation

    fusion of CITED2 and Hif1a

    Promoter

    T7

    Available Since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. HisGB1-FusionL21A + CBP TAZ1(340-439)

    Plasmid

    #173763

    Purpose

    Bacterial Coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructs

    Depositor

    Peter Wright

    Article

    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(

    Inserts

    CITED2(216-246L231A):Hif1a(796-826) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    

    Use

    Tags

    His6GB1 and thrombin

    Expression

    Bacterial

    Mutation

    fusion of CITED2 and Hif1a

    Promoter

    T7

    Available Since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. HIsGB1-FusionL63A + CBP TAZ1(340-439)

    Plasmid

    #173764

    Purpose

    Bacterial coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructs

    Depositor

    Peter Wright

    Article

    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(

    Inserts

    CITED2(216-246):Hif1a(796-826L822A) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    

    Use

    Tags

    His6GB1 and thrombin

    Expression

    Bacterial

    Mutation

    fusion of CITED2 and Hif1a

    Promoter

    T7

    Available Since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL4-eIF3B MBE3 mutant

    Plasmid

    #133310

    Purpose

    Expression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 3

    Depositor

    Josep Domingo-Domenech

    Article

    Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

    Insert

    eIF3B promoter MBE3 mutant (EIF3B Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    ccacgtgacc changed to cAaAAAAacc

    Promoter

    eIF3B

    Available Since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL4-eIF3B MBE2 mutant

    Plasmid

    #133309

    Purpose

    Expression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 2

    Depositor

    Josep Domingo-Domenech

    Article

    Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

    Insert

    eIF3B promoter MBE2 mutant (EIF3B Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    gccacatgcacc changed to gcAaAaAAcacc

    Promoter

    eIF3B

    Available Since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDONR221-TAF15 g1172a No Stop

    Plasmid

    #84898

    Purpose

    DONOR vector for Gateway cloning of TAF15 g1172a No Stop

    Depositor

    Aaron Gitler

    Article

    Couthouis et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

    Insert

    TAF15 (TAF15 Human)
    

    Use

    Gateway donor

    Tags

    Expression

    Mutation

    g1172a No Stop

    Promoter

    none

    Available Since

    April 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-EML4-ALK variant 3a I1171N

    Plasmid

    #183832

    Purpose

    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK I1171N mutation in mammalian cells

    Depositor

    Aaron Hata

    Article

    Shiba-Ishii et al Nat Cancer. 2022 Jun 20. pii: 10.1038/s4

    Inserts

    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    

    Use

    Lentiviral

    Tags

    The fusion protein of EML4 exon 1-6a and ALK exon…

    Expression

    Mammalian

    Mutation

    I1171N

    Promoter

    CMV

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-CD74-ROS1 G2032R/L2086F

    Plasmid

    #183820

    Purpose

    Express CD74-ROS1 fusion (C6;R34) harboring ROS1 G2032R/L2086F mutation in mammalian cells

    Depositor

    Aaron Hata

    Article

    Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

    Inserts

    ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
    CD74 molecule (CD74 Human)
    

    Use

    Lentiviral

    Tags

    The fusion protein of CD74 exon 1-6 and ROS1 exon…

    Expression

    Mammalian

    Mutation

    G2032R/L2086F

    Promoter

    CMV

    Available Since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-CD74-ROS1 S1986F/G2032R/L2086F

    Plasmid

    #183822

    Purpose

    Express CD74-ROS1 fusion (C6;R34) harboring ROS1 S1986F/G2032R/L2086F mutation in mammalian cells

    Depositor

    Aaron Hata

    Article

    Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

    Inserts

    ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
    CD74 molecule (CD74 Human)
    

    Use

    Lentiviral

    Tags

    The fusion protein of CD74 exon 1-6 and ROS1 exon…

    Expression

    Mammalian

    Mutation

    S1986F/G2032R/L2086F

    Promoter

    CMV

    Available Since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-CD74-ROS1 S1986F/G2032R

    Plasmid

    #183821

    Purpose

    Express CD74-ROS1 fusion (C6;R34) harboring ROS1 S1986F/G2032R mutation in mammalian cells

    Depositor

    Aaron Hata

    Article

    Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

    Inserts

    ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
    CD74 molecule (CD74 Human)
    

    Use

    Lentiviral

    Tags

    The fusion protein of CD74 exon 1-6 and ROS1 exon…

    Expression

    Mammalian

    Mutation

    S1986F/G2032R

    Promoter

    CMV

    Available Since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLenti-CD74-ROS1 S1986F/L2000V

    Plasmid

    #183818

    Purpose

    Express CD74-ROS1 fusion (C6;R34) harboring ROS1 S1986F/L2000V mutation in mammalian cells

    Depositor

    Aaron Hata

    Article

    Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

    Inserts

    ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
    CD74 molecule (CD74 Human)
    

    Use

    Lentiviral

    Tags

    The fusion protein of CD74 exon 1-6 and ROS1 exon…

    Expression

    Mammalian

    Mutation

    S1986F/L2000V

    Promoter

    CMV

    Available Since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLenti-CD74-ROS1 S1986F/L2086F

    Plasmid

    #183819

    Purpose

    Express CD74-ROS1 fusion (C6;R34) harboring ROS1 S1986F/L2086F mutation in mammalian cells

    Depositor

    Aaron Hata

    Article

    Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

    Inserts

    ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
    CD74 molecule (CD74 Human)
    

    Use

    Lentiviral

    Tags

    The fusion protein of CD74 exon 1-6 and ROS1 exon…

    Expression

    Mammalian

    Mutation

    S1986F/L2086F

    Promoter

    CMV

    Available Since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV5D HA-PPM1H_M flap

    Plasmid

    #236718

    Purpose

    Expresses PPM1H with PPM1M flap domain sequence

    Depositor

    Suzanne Pfeffer

    Article

    Claire Y. et al bioRxiv, 2025

    Insert

    PPM1H_M flap (PPM1H, PPM1M Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    PPM1H flap domain replaced with PPM1M flap domain

    Promoter

    CMV

    Available Since

    May 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV5D HA-PPM1M_H flap

    Plasmid

    #236719

    Purpose

    Expresses PPM1M with PPM1H flap domain sequence

    Depositor

    Suzanne Pfeffer

    Article

    Claire Y. et al bioRxiv, 2025

    Insert

    PPM1M_H flap (PPM1M, PPM1H Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    PPM1M flap domain replaced with PPM1H flap domain

    Promoter

    CMV

    Available Since

    May 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. MoChlo: Modular Cloning Chloroplast Toolbox

    Plasmid Kit

    #1000000156

    Purpose

    The MoChlo Toolbox consists of a library of standardized chloroplast-specific genetic modules for Golden-Gate cloning of synthetic operons into chloroplast transformation vectors.

    Depositor

    Scott Lenaghan

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

26. pLenti-EML4-ALK variant 3a I1171N/D1203N

    Plasmid

    #183833

    Purpose

    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK I1171N/D1203N mutation in mammalian cells

    Depositor

    Aaron Hata

    Article

    Shiba-Ishii et al Nat Cancer. 2022 Jun 20. pii: 10.1038/s4

    Inserts

    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    

    Use

    Lentiviral

    Tags

    The fusion protein of EML4 exon 1-6a and ALK exon…

    Expression

    Mammalian

    Mutation

    I1171N/D1203N

    Promoter

    CMV

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. BJ5183 cells

    Bacterial Strain

    #16398

    Depositor

    Bert Vogelstein

    Article

    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

    Bacterial Resistance

    Streptomycin

    Species

    Escherichia coli

    Available Since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

28. CRBN_ΔHBD

    Plasmid

    #232779

    Purpose

    Expresses CRBN_ΔHBD sequence in E. coli cells with cleavable MBP-His tag and deleted internal DDB1 binding domain

    Depositor

    Ivan Dikic

    Article

    Bailey et al Cell Chem Biol. 2024 Nov 20:S2451-9456(2

    Insert

    Cereblon (CRBN Human)
    

    Use

    Tags

    MBP-His6-TEV

    Expression

    Bacterial

    Mutation

    CRBN (residues 47 to 193 and 249 to 436) with GNG…

    Promoter

    T7

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHis-TEV-Nsp15-E266Q

    Plasmid

    #218803

    Purpose

    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-E266Q (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

    Depositor

    Debra Hansen

    Article

    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:

    Insert

    Nsp15-E266Q (N Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    TEV protease cleavable His6-tag

    Expression

    Bacterial

    Mutation

    E266Q (G to C)

    Promoter

    Available Since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHis-TEV-Nsp15-V66L

    Plasmid

    #219524

    Purpose

    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-V66L (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

    Depositor

    Debra Hansen

    Article

    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:

    Insert

    Nsp15-V66L (N Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    TEV protease cleavable His6-tag

    Expression

    Bacterial

    Mutation

    V66L (G to C)

    Promoter

    Available Since

    May 27, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pHis-TEV-Nsp15-A81V

    Plasmid

    #219525

    Purpose

    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-A81V (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

    Depositor

    Debra Hansen

    Article

    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:

    Insert

    Nsp15-A81V (N Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    TEV protease cleavable His6-tag

    Expression

    Bacterial

    Mutation

    A81V (C to T)

    Promoter

    Available Since

    May 27, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pHis-TEV-Nsp15-D183N

    Plasmid

    #219526

    Purpose

    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-D183N (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

    Depositor

    Debra Hansen

    Article

    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:

    Insert

    Nsp15-D183N (N Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    TEV protease cleavable His6-tag

    Expression

    Bacterial

    Mutation

    D183N (G to A)

    Promoter

    Available Since

    May 27, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pHis-TEV-Nsp15-H234A

    Plasmid

    #219529

    Purpose

    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-H234A (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

    Depositor

    Debra Hansen

    Article

    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:

    Insert

    Nsp15-H234A (N Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    TEV protease cleavable His6-tag

    Expression

    Bacterial

    Mutation

    H234A (CAC to GCG)

    Promoter

    Available Since

    May 27, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. V. natriegens NC7

    Bacterial Strain

    #215356

    Purpose

    Vibrio natriegens with genomically integrated natural competence regulator, TfoX. For zero-capital molecular biology applications.

    Depositor

    Buz Barstow

    Article

    Specht et al PNAS Nexus. 2024 Feb 13;3(2):pgad444. do

    Bacterial Resistance

    None

    Available Since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

35. V. natriegens NC1

    Bacterial Strain

    #215355

    Purpose

    Vibrio natriegens with genomically integrated natural competence regulator, TfoX, with chloramphenicol resistance. For zero-capital molecular biology applications.

    Depositor

    Buz Barstow

    Article

    Specht et al PNAS Nexus. 2024 Feb 13;3(2):pgad444. do

    Bacterial Resistance

    None

    Available Since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

36. FNL Combinatorial Cloning Platform

    Plasmid Kit

    #1000000211

    Purpose

    A collection of Multisite Gateway Entry clone plasmids which can be used in a combinatorial fashion to generate large numbers of tagged expression clones for protein expression in a variety of systems

    Depositor

    Dominic Esposito

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

37. pTKA001

    Plasmid

    #164577

    Purpose

    Expresses SARS-CoV-2 nsp7 from a pET46 backbone for IPTG-inducible bacterial expression

    Depositor

    Robert Kirchdoerfer

    Article

    Altincekic et al Front Mol Biosci. 2021 May 10;8:653148.

    Insert

    nsp7 (ORF1ab )
    

    Use

    Tags

    6xHis, Ek, TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTKA002

    Plasmid

    #164578

    Purpose

    Expresses SARS-CoV-2 nsp8 from a pET46 backbone for IPTG-inducible bacterial expression

    Depositor

    Robert Kirchdoerfer

    Article

    Altincekic et al Front Mol Biosci. 2021 May 10;8:653148.

    Insert

    nsp8
    

    Use

    Tags

    6xHis, Ek, TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGJK_His-SUMO-LbuCas13a

    Plasmid

    #172488

    Purpose

    Bacterial expression of codon-optimized His-SUMO-LbuCas13a.

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

    Insert

    bdSUMO-LbuCas13a (codon-optimized)
    

    Use

    Tags

    His6 and bdSUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter and lac operator

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pOPINB

    Plasmid

    #41142

    Depositor

    Ray Owens

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pOPINA

    Plasmid

    #41141

    Depositor

    Ray Owens

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. Top7 H1-H2

    Plasmid

    #12479

    Depositor

    David Baker

    Insert

    Top7 H1-H2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    H1-H2

    Promoter

    Available Since

    March 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. Top7 S2-H2

    Plasmid

    #12477

    Depositor

    David Baker

    Insert

    Top7 S2-H2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S2-H2

    Promoter

    Available Since

    March 13, 2007

    Availability

    Academic Institutions and Nonprofits only

44. Top7 S2-S4

    Plasmid

    #12478

    Depositor

    David Baker

    Insert

    Top7 S2-S4
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S2-S4

    Promoter

    Available Since

    March 13, 2007

    Availability

    Academic Institutions and Nonprofits only

45. Top7 H1-S4

    Plasmid

    #12480

    Depositor

    David Baker

    Insert

    Top7 H1-S4
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    H1-S4

    Promoter

    Available Since

    March 13, 2007

    Availability

    Academic Institutions and Nonprofits only

46. pOPINK

    Plasmid

    #41143

    Depositor

    Ray Owens

    Insert

    GST
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. FDA60

    Plasmid

    #45606

    Depositor

    David Baker

    Insert

    FDA60
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

48. pCS2HA

    Plasmid

    #16330

    Depositor

    Peter Klein

    Insert

    HA tag
    

    Use

    Vertebrate expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

49. FR52re

    Plasmid

    #45489

    Depositor

    David Baker

    Insert

    FR52re
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    redesign of Di-I_5_FR28

    Promoter

    Available Since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

50. ssCFr

    Plasmid

    #12481

    Depositor

    David Baker

    Insert

    ssCFr
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 13, 2007

    Availability

    Academic Institutions and Nonprofits only