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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 23,588 results for: CHI

Showing: 11201 - 11250 of 23588 results

  23. pHIT186

    Plasmid
    #204513
    Purpose
    Bacterial expression of CSR-1 PAZ domain with 6xHis and maltose binding protein tags for use as an antigen
    Depositor
    Yuji Kohara
    Article
    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:
    Insert
    PAZ domain of csr-1 (C. elegans) (csr-1 Nematode)
    Use
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Available since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    204513-plasmid-map-sequence-id-403809

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  24. pHIT520

    Plasmid
    #204519
    Purpose
    Bacterial expression of CEC-8 N-terminal fragment with 6xHis and maltose binding protein tags for in vitro assays
    Depositor
    Yuji Kohara
    Article
    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:
    Insert
    N-terminal fragment of cec-8 (C. elegans) (Y55B1BR.3 Nematode)
    Use
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Available since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    204519-plasmid-map-sequence-id-403744

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  25. pHIT320

    Plasmid
    #204517
    Purpose
    Bacterial expression of HIM-1 internal fragment with 6xHis and maltose binding protein tags for use as an antigen
    Depositor
    Yuji Kohara
    Article
    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:
    Insert
    Internal fragment of him-1 (C. elegans) (him-1 Nematode)
    Use
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Available since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    204517-plasmid-map-sequence-id-403742

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  31. pTP-PCR C1

    Plasmid
    #189618
    Purpose
    Cloned Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria
    Depositor
    Bogumil Karas
    Article
    Cochrane et al Biology (Basel). 2020 Oct 26;9(11). pii:
    Insert
    Mitochondrial Genome of Thalassiosira pseudonana
    Use
    Synthetic Biology; Diatom expression
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Minor additional mutations described in manuscript
    Promoter
    Available since
    July 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    189618-plasmid-map-sequence-id-401652

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  32. pCAGGS-C-TEV-HAT

    Plasmid
    #202597
    Purpose
    Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Type
    Empty backbone
    Use
    Tags
    TEV cleavable site (ENLYFQG) and poly-histidine a…
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202597-plasmid-map-sequence-id-399552

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  33. pCAGGS-N-TEV-HAT

    Plasmid
    #202598
    Purpose
    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Type
    Empty backbone
    Use
    Tags
    TEV cleavable site (ENLYFQG) and poly-histidine a…
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202598-plasmid-map-sequence-id-399468

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  34. OsMTI-1B Oryza sativa Metallothionein 1B

    Plasmid
    #200295
    Purpose
    Bacterial expression plasmids for production of recombinant Oryza sativa Metallothionein 1B
    Depositor
    Artur Krężel
    Article
    Mosna et al Metallomics. 2023 Oct 4;15(10):mfad061.
    Insert
    Gene of metallothionein with Chiting Binding Domanin-Intein (LOC4332487 Oryza sativa)
    Use
    Tags
    Chiting Binding Domanin-Intein
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    200295-plasmid-map-sequence-id-394827

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  35. pJL1-MBP-DQNAT

    Plasmid
    #198215
    Purpose
    pJL1 plasmid encoding E. coli maltose binding protein (MBP) modified at the C-terminus with 30 amino acids containing one optimal DQNAT sequon followed by a 6x-His tag
    Depositor
    Michael Jewett
    Article
    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:
    Insert
    MBP_DQNAT
    Use
    Tags
    6x His Tag and DQNAT glycosylation tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    198215-plasmid-map-sequence-id-393681

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  36. pJL1-PD-DQNAT

    Plasmid
    #198216
    Purpose
    pJL1 plasmid encoding H. influenzae protein D (PD) modified at the C-terminus with 30 amino acids containing one optimal DQNAT sequon followed by a 6x-His tag
    Depositor
    Michael Jewett
    Article
    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:
    Insert
    PD-DQNAT
    Use
    Tags
    6x His Tag and DQNAT glycosylation tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    198216-plasmid-map-sequence-id-393680

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  37. pTP-PCR C4

    Plasmid
    #189621
    Purpose
    Cloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria
    Depositor
    Bogumil Karas
    Article
    Cochrane et al Biology (Basel). 2020 Oct 26;9(11). pii:
    Insert
    Reduced Mitochondrial Genome of Thalassiosira pseudonana
    Use
    Synthetic Biology; Diatom expression
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Repeat region removed, and minor additional mutat…
    Promoter
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    189621-plasmid-map-sequence-id-379021

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  38. pTP-PCR C2

    Plasmid
    #189619
    Purpose
    Cloned Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria
    Depositor
    Bogumil Karas
    Article
    Cochrane et al Biology (Basel). 2020 Oct 26;9(11). pii:
    Insert
    Mitochondrial Genome of Thalassiosira pseudonana
    Use
    Synthetic Biology; Diatom expression
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Minor additional mutations described in manuscript
    Promoter
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    189619-plasmid-map-sequence-id-379034

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  40. NeuA.pJL1

    Plasmid
    #199115
    Purpose
    In vitro expression of N-acylneuraminate cytodiylyltransfease (NeuA), which conjugates sialic acid and CTP to make activated sugar donor, CMP-sialic acid (see CSTI.PJL1 and PdST6.PJL1)
    Depositor
    Michael Jewett
    Article
    Thames et al ACS Synth Biol. 2023 Apr 11. doi: 10.102
    Insert
    N-acylneuraminate cytodiylyltransfease (NeuA)
    Use
    Tags
    Strep-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    199115-plasmid-map-sequence-id-390797

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Showing: 11201 - 11250 of 23588 results