178,239 results
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Plasmid#189922PurposeAAV genome encoding SaABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installationDepositorInsertSaABE8e
UseAAVMutationSaCas9 D10APromoterEFSAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-PEmax
Plasmid#187893PurposeAll-in-one prime editor piggyBac transposon with PEmaxDepositorInsertSpCas9_H840A_KK-MMLV-RT_co-P2A-PAC_dTK
UseCRISPR and Synthetic Biology; Piggybac transposonTagsBPSV40 NLS and c-Myc NLSExpressionMammalianPromoterCAGAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1_3XFlag
Plasmid#182494PurposeEmpty plasmid containing Flag tag for C-terminal taggingDepositorInsert3xFlag with 5 Glycolic spacer
Available SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
AKT-translocation-mStrawberry reporter
Plasmid#158685PurposeThis plasmid is a AKT pathway reporter. It has 1EF1a promoter driving the expression of truncated FoxO1 fused to mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EML4-ALK variant 1
Plasmid#183828PurposeExpress EML4-ALK fusion variant 1 (E13;A20) in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of EML4 exon 1-13 and ALK exon…ExpressionMammalianPromoterCMVAvailable SinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
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GPI-RpHLuorin2
Plasmid#171721PurposeGPI-anchored RpHLuorin2 for plasma membrane expression (extracellular)DepositorInsertRatiometric pHLuorin2 fused to GPI anchor
Tagsratiometric pHluorin2ExpressionMammalianPromoterCMVAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynap.(cyto)iGlucoSnFR-mRuby2 (AAV9)
Viral Prep#164510-AAV9PurposeReady-to-use AAV9 particles produced from pAAV.hSynap.(cyto)iGlucoSnFR-mRuby2 (#164510). In addition to the viral particles, you will also receive purified pAAV.hSynap.(cyto)iGlucoSnFR-mRuby2 plasmid DNA. Synapsin-driven, cytosolic-targeted iGlucoSnFR-mRuby2DepositorPromoterSynTagsmRuby2Available SinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-dCasRx-METTL3-PURO
Plasmid#174218PurposeExpresses dCasRx-METTL3 in mammalian cellDepositorInsertdCasRx-METTL3
UseCRISPR and LentiviralExpressionMammalianPromoterpMSCVAvailable SinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-dCas9-VPR-H2B-GFP (CRISPRa)
Plasmid#175571PurposeInactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activationDepositorTypeEmpty backboneUseCRISPRAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
NW11
Plasmid#133443PurposeCovalently circularized nanodisc NW11 for studying membrane proteins and viral entry.DepositorInsertNW11
ExpressionBacterialAvailable SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-sRGECO (AAV8)
Viral Prep#137125-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-Ef1a-sRGECO (#137125). In addition to the viral particles, you will also receive purified pAAV-Ef1a-sRGECO plasmid DNA. EF1a-driven expression of cytosolic sRGECO calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aAvailable SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
AiP11390-pAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1390)
Plasmid#163505PurposeDLX2.0 (3xCore version of DLX enhancer). AAV vector for strong & rapid SYFP2 expression in forebrain GABAergic interneurons. The enhancer core has 100% seq conservation between mouse and humanDepositorHas ServiceAAV PHP.eBInsertSYFP2
UseAAVPromoterminBetaGlobinAvailable SinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19_CRISPR_DpmrA
Plasmid#160903PurposeCRISPR_Cas9, lambda red recombineeringDepositorInsertsCas9
Lambda Red Recombineering Genes
Zeocin Resistance Cassette
PromoteraraBADAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGIPz-mPD-L1
Plasmid#121488PurposeExpression of mouse PD-L1 WTDepositorInsertPD-L1
UseLentiviralTagsFlagExpressionMammalianPromoterCMVAvailable SinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGFP(HiBiT)
Plasmid#162589PurposeN-terminally HiBiT-tagged EGFP under CAG promoterDepositorInsertEGFP
TagsHiBiTExpressionMammalianPromoterCAG promoterAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EGFP-IRES-Neo
Plasmid#128660Purposemammalian expression of EGFP with neomycin selectionDepositorInsertEGFP
UseLentiviralTagsN/AExpressionMammalianMutationWild typePromoterCMVAvailable SinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-NIR-GECO2G
Plasmid#159605PurposeAAV production plasmid encoding for near-infrared calcium indicator NIR-GECO2GDepositorHas ServiceAAV1, AAV2, and AAV9InsertNIR-GECO2G
UseAAVExpressionMammalianPromoterCAGAvailable SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.SF-iGluSnFR.A184S (AAV1)
Viral Prep#106192-AAV1PurposeReady-to-use AAV1 particles produced from pAAV.GFAP.SF-iGluSnFR.A184S (#106192). In addition to the viral particles, you will also receive purified pAAV.GFAP.SF-iGluSnFR.A184S plasmid DNA. GFAP-driven expression of SF-iGluSnFR.A184S. These AAV preparations are suitable purity for injection into animals.DepositorPromoterGFAPAvailable SinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E6-dTom-nlsdTom
Plasmid#135641PurposeAAV vector to drive the expression of dTomato in VIP cortical interneurons under the control of the E6 regulatory elementDepositorHas ServiceAAV1InsertdTom-nlsdTom
UseAAVMutationNoneAvailable SinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E2-GCaMP6f
Plasmid#135632PurposeAAV vector to drive the expression of GCaMP6f in PV cortical interneuronsunder the control of the E2 regulatory elementDepositorHas ServiceAAV PHP.eB, AAV1, and AAV9InsertGCaMP6f
UseAAVMutationNoneAvailable SinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
LiOn*-CAG∞RFP
Plasmid#154019PurposeVector based on the LiOn integration-coupled translational switch and devoid of TTAA sequences, expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposaseDepositorInsertmRFP1
ExpressionMammalianMutationAll TTAA in vector backbone were replaced by sile…PromoterCAGAvailable SinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-NLS-dPspCas13b-3xsfGFP-NLS
Plasmid#132404Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralExpressionMammalianMutationH133A; H1059AAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGM55261
Plasmid#153210PurposeLevel 2 cloning vector for a single guide RNA, already contains Cas9, Bar selection cassette and FAST markerDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas13-inactive M3nls
Plasmid#157854PurposeTargeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutantDepositorInsertdCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
ExpressionMammalianMutationPspCas13b Δ984-1090 H133A; METTL3 D395AAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_rDA1m
Plasmid#140556PurposeExpresses the red fluorescent medium-affinity DA sensor GRAB_rDA1m in neuronsDepositorHas ServiceAAV9InsertRed fluorescent DA sensor GRAB_rDA1m (medium affinity)
UseAAVPromoterhSynAvailable SinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUBC_mCherry_serinemod_E488QADAR_p2A_yGFP
Plasmid#154786PurposeExpresses mCherry fused to hyperactive ADAR catalytic domain, control plasmid for HyperTRIBEDepositorInsertAdenosine deaminse (Adar Fly, Synthetic)
UseLentiviralTagsGFP, V5, and mCherryExpressionMammalianMutationSynonymized serine to prevent autoediting of ADAR…PromoterUBCAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphai1-RLuc8
Plasmid#140973PurposeEncodes a G alpha subunit (GNAl1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphai1-RLuc8 (GNAI1 Human)
UseSynthetic BiologyExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
Human sgRNA library Gattinara in pRDA_118 backbone
Pooled Library#136986PurposeDesigned for assays with limited cell numbers with 2 guides per gene. Compatible with John Doench’s Brunello human genome-wide library.DepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable SinceFeb. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
hCMV-IE1:Renilla
Plasmid#118066PurposeTranscriptional unit for constitutive expression of Renilla in mammalian cellsDepositorInsertCMV:GreenRenilla:bGHF
UseLuciferase and Synthetic Biology; Assembly of gb2…ExpressionMammalianPromoterCMVAvailable SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-UFM1
Plasmid#134639Purposeexpression of HA-tagged human UFM1 wildtypeDepositorAvailable SinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mCherry-CaM
Plasmid#127393PurposeFluorescent reporter for calmodulin 1DepositorAvailable SinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE (AAV Retrograde)
Viral Prep#104495-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE (#104495). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE plasmid DNA. CAG-driven, Cre-dependent GCaMP7s calcium sensor. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGAvailable SinceOct. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-YFP-miniTurbo-L2
Plasmid#127352Purposegateway entry vector for making N-terminal YFP-miniTurbo fusions with nuclear and non-nuclear proteinsDepositorInsertminiTurbo (BirA mutant)
UseGateway CloningTagsGS linker, V5, and YFPMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Promoterno promoterAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE
Plasmid#130995PurposeAAV vector to drive the expression of soma-targeted ChRmine-mScarlet under the control of human synapsin promoterDepositorHas ServiceAAV8InsertChRmine
UseAAVTagsmScarlet-Kv2.1PromoterhSynAvailable SinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-2E2-HA-mCh
Plasmid#129596PurposeThe encoded protein is the anti-HA frankenbody variant fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.DepositorInsertAnti-HA frankenbody variant-mCherry (2E2-HA scFv-mCherry)
ExpressionMammalianPromoterCMVAvailable SinceAug. 27, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLV-CAG-FLEX-BFP2-(mCherry)’
Plasmid#115234PurposeLentiviral transfer plasmid for expression of mTagBFP2 before Cre recombination and mCherry after itDepositorInsertmTagBFP2 and mCherry
ExpressionMammalianAvailable SinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Guide-Zeo
Plasmid#127173PurposeEntry plasmid for guide cloningDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterEF1aAvailable SinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 hMOG-alpha1-EGFP
Plasmid#126463PurposeExpression a human MOG (isoform alpha1) EGFP fusion protein in mammalian cellsDepositorAvailable SinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP104-melanopsin-mCherry
Plasmid#122630PurposeAAV-mediated expression of melanopsin-mCherry under the GFAP promoterDepositorInsertmelanopsin-mCherry
UseAAVTagsmCherryExpressionMammalianPromoterGFAP104Available SinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-Bxb1-His
Plasmid#123132PurposeBacterial expression plasmid for wild-type Bxb1 with a C-terminal His-tag.DepositorInsertBxb1 integrase
Tags6x His tagExpressionBacterialPromoterT7Available SinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCFD3.1-w-dU6:3gRNA
Plasmid#123366PurposegRNA expression plasmid for CRISPR mutagenesis in Drosophila (with mini-white transformation marker).DepositorInsertU6:3-gRNA
UseCRISPRExpressionInsectAvailable SinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Floxed-STOP Fluc
Plasmid#122962PurposeFirefly luciferase (Fluc)-based bioluminescent reporter for Cre-loxP DNA recombination reactionDepositorInsertpcDNA3.1_Floxed-STOP Fluc
UseCre/Lox and LuciferaseExpressionMammalianPromoterCMVAvailable SinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
negMaLionR
Plasmid#113909Purposenegative control for Red ATP sensorDepositorInsertnegMaLionR
ExpressionMammalianAvailable SinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEG302 22aa SunTag VP64 nog
Plasmid#120251PurposeCRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide)DepositorInsertNOS_NLS_GB1_NLS_linker_VP64_linker sfGFP_scFv_UBQ10_Insulator_ UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-COX4-dAPEX2
Plasmid#117177PurposePlasmid name in publication: pAAV-DIO-Matrix-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertCOX4-dAPEX2
UseAAV and Cre/LoxExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailable SinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FGFR3
Plasmid#116742PurposeLentiviral expression of FGFR3DepositorAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynapsin.SF-iGluSnFR.S72A (AAV1)
Viral Prep#106176-AAV1PurposeReady-to-use AAV1 particles produced from pAAV.hSynapsin.SF-iGluSnFR.S72A (#106176). In addition to the viral particles, you will also receive purified pAAV.hSynapsin.SF-iGluSnFR.S72A plasmid DNA. hSynapsin driven SF-iGluSnFR.S72A, glutamate sensor. The S72A mutant has lower affinity for glutamate. Slower on-rate and faster off-rate. Enhanced temporal resolution and spatial resolution. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceFeb. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLH-sgRNA-Sirius-4X(MS2-PP7)
Plasmid#121941PurposeCRISPR-Sirius plasmidDepositorInsertsgRNA-Sirius-4X(MS2-PP7)
UseCRISPR and LentiviralTagsnoExpressionMammalianPromoterhuman U6 promoterAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
SynTagMA_pre
Plasmid#119738PurposeSynaptic Tag for Mapping ActivityDepositorInsertSynTagMA_pre
UseAAVExpressionMammalianPromoterhsyn1Available SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only