161,697 results
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Plasmid#163524PurposeExpresses both eGFP and chicken ovalbumin in mammalian cellsDepositorInsertenhanced-GFP-chicken ovalbumin tandem
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.iGluSnFR3.v857.GPI
Plasmid#178335PurposeFluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPIDepositorInsertpAAV CAG iGluSnFR3 v857.GPI
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsGPI Anchor, IgK-chain, and Myc epi tagExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-nbMmIgG1-Tn5
Plasmid#184287PurposeAnti-mouse IgG1 Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)DepositorInsertnbMmIgG1-Tn5
UseTagsExpressionMutationPromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-LivePAR-Hygro
Plasmid#176063PurposeEGFP fused to the C-terminus of a WWE domain & a hygromycin resistance cassetteDepositorInsertLivePAR
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1AAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEM4Z-EGFP
Plasmid#183475PurposeThis plasmid can be used to prepare EGFP mRNA by In Vitro TranscriptionDepositorInsertEGFP
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDule2 - Mj Acd A9
Plasmid#197652PurposeE. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pairDepositorInsertsMj Acd A9 synthetase
Methanocaldococcus jannaschii tRNA
UseTagsNoneExpressionBacterialMutationY32A, L65D, F108L, Q109E, D158S, L162TPromoterGlnS and lppAvailable sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19/PLIN2-GFP
Plasmid#202194Purposeknock-in donor vector of GFP to the C-terminus of endogenous human PLIN2DepositorInsertPLIN2 (PLIN2 Human)
UseCloning vectorTagsGFP knock-inExpressionMutationPromoterlacAvailable sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEG BacMam C term 3C eGFP His8
Plasmid#160687PurposeVector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target proteinDepositorTypeEmpty backboneUseTags3C eGFP His8ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZS47a luxI-laa RBS
Plasmid#48892PurposeArsR-regulated expression of luxI-laaDepositorInsertLuxI
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpArsRAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA GNSTM-3-Flag-10-Lamp2b-HA
Plasmid#71293PurposeEncodes (N to C): GNSTM glycosylation motif, 3 residue spacer, Flag tag,10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.DepositorInsertLamp2b (LAMP2 Human)
UseTagsFLAG, GNSTM glycosylation motif, HA, and Lamp2 si…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
Myc-Foxo1 AAA (pCMV5)
Plasmid#17547DepositorInsertFoxo1 AAA (Foxo1 Mouse)
UseTagsMycExpressionMammalianMutationChanged T24A T253A S316APromoterCMVAvailable sinceOct. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
V2-STING
Plasmid#124265PurposeN-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartmentsDepositorInsertTMEM173 (STING1 Human)
UseTagsVenus2ExpressionMammalianMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF2258
Plasmid#142927PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSOX11 (SOX11 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAM5505
Plasmid#132664PurposepRL623-based helper plasmid for conjugations, with RSF1010 mobA geneDepositorInsertMobA
UseTagsExpressionBacterialMutationplease see depositor comments belowPromoterp1,p3Available sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Hygro DEST H2B-mRuby2
Plasmid#90236PurposeLentiviral vector to express H2B-mRuby2 under PGK promoter (With Hygromycin Resistance)DepositorInsertH2B
UseLentiviralTagsmRuby2ExpressionMammalianMutationPromoterPGKAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMK003
Plasmid#236570PurposeCloning vector for PaqCI Golden Gate assembly of ISDra2 guides into ISDra2 plant expression vectorDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiLAMP5e3_Gq_dTomato
Plasmid#213916PurposeAAV construct expressing HA-hM3Dq-dTomato driven by Lamp5 interneuron-targeting enhancer. Alias: pAAV_WDL0003_Gq_dTomatoDepositorInsertHA-GqDREADD-dTomato
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
MsACR1-mCerulean3-pAAV2-hSyn
Plasmid#204965PurposePanneuronal expression of MsACR1 fused to mCerulean3DepositorInsertMsACR1
UseAAVTagsmCerulean3ExpressionMutationPromoterhSynAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
MsACR1-TS-Kv-P2A-mCerulean3-pAAV-CaMKII
Plasmid#204970PurposeSoma-targeted neuronal expression of MsACR1 and mCerulean3 separated by a P2A site.DepositorInsertMsACR1
UseAAVTagsmCerulean3ExpressionMutationPromoterCaMKIIAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
p8328 pLIX YAP1 S94A
Plasmid#184529PurposeExpression of YAP1 S94ADepositorInsertYAP1 S94A (YAP1 Human)
UseLentiviralTagsExpressionMutationS94APromoterTRE promoter, Tet ONAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
HIV-dual-GT
Plasmid#122696PurposeDual-fluorescence reporter to monitor the expression of HIV-1 early genes and late genes simultaneously.DepositorInsertsmTagBFP2
DsRed-max
UseLentiviralTagsPEST domainExpressionMutationPromoterHIV-1 LTRAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR_Puro_sgTP53
Plasmid#187819PurposepLentiCRISPR that is selectable with puromycin with an sgRNA targeting TP53DepositorInsertsgTP53
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGWB533
Plasmid#74872PurposeGateway cloning compatible binary vector for C-terminal fusion with GUS (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2A
Plasmid#15780DepositorInsertUbiquitin conjugating enzyme UbE2A (UBE2A Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHR-SFFV-dCas9-BFP
Plasmid#46910PurposeHuman expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS and tagBFPDepositorInsertdCas9-BFP fusion
UseCRISPR and LentiviralTags2xNLS, BFP, and HAExpressionMammalianMutationPromoterSFFVAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
Human SAM Library in lenti sgRNA(MS2)_zeo backbone
Pooled Library#1000000057PurposeGenome-wide CRISPR gRNA pooled library for activation of human genesDepositorAvailable sinceJan. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
TRE-VEGFA EF1a-rtTA3
Plasmid#195881PurposeLentiviral construct of doxycycline inducible VEGFADepositorInsertVEGFA (Vegfa Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
OPN(-1206)-luc
Plasmid#31106DepositorInsertosteopontin promoter (SPP1 Human)
UseLuciferaseTagsExpressionMammalianMutationpromoter region -1206 to +87PromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
TFORF1938
Plasmid#141892PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMEIS1 (MEIS1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Talin1 head EGFP fusion
Plasmid#221408PurposeMammalian expression of Talin1 head EGFP fusion for mammalian expressionDepositorInsertTalin1 head EGFP fusion (TLN1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
Plasmid L5 attB::Pleft* mScarlet
Plasmid#169410PurposeChromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mScarletDepositorInsertspLeft
mScarlet proper
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-YR-ICUE-mGold2s
Plasmid#231789PurposeVisualization of cAMP dynamics using YR-ICUE-mGold2sDepositorInsertYR-ICUE-mGold2s
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pN7-S15
Plasmid#190665PurposeExpresses N7 ribosomal protein S15DepositorInserthuman codon optimized N7 S15
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
5HT6-mCherry-G-GECO1.0
Plasmid#47500PurposeFluorescent reporter for Ca2+ inside primary cilia with mCherry referenceDepositorInsertG-GECO1.0
UseTags5HT6 and mCherryExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCSC-NLS-EGFP
Plasmid#221811PurposeLentiviral vector expressing green fluorescent protein (GFP) fused with nuclear localization sequence (NLS)DepositorInsertNLS-EGFP
UseLentiviralTagsNLSExpressionMammalianMutationPromoterCMVAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
EB3N-VVDfast-Halo-iLID
Plasmid#190166PurposeExpresses EB3N-VVDfast-Halo-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.DepositorInsertEB3N-VVDfast-Halo-iLID
UseTags-VVDfast-mClover3-iLIDExpressionMammalianMutation1-189 aa from EB3PromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Tsg101
Plasmid#21505DepositorInsertTsg101 (TSG101 Human)
UseTagsCherry*ExpressionBacterialMutationnonePromoterAvailable sinceSept. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
GFP-C1-PKCgamma-C1A
Plasmid#21205DepositorInsertPKCgamma-C1A (Prkcg Rat)
UseTagsGFPExpressionMammalianMutationPKCgamma-C1A domain. (Lab plasmid WC 0018)PromoterAvailable sinceJuly 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pYLCRISPR/Cas9P35S-H
Plasmid#66189Purposeexpression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), hygro selectionDepositorInsertCas9
UseTagsExpressionPlantMutationplant-codon optimized with higher GC contents (62…Promotercauliflower mosaic virus 35S promoter (P35S)Available sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-ON-shCtrl (neo)
Plasmid#192076PurposeLentivirus for Dox-inducible expression of a non-targeting control shRNADepositorInsertnon-targeting randomized sequence
UseLentiviralTagsExpressionMutationPromoterH1 / Tet-responsive elementAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-MBP
Plasmid#133450PurposeExpresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasmDepositorInsertSpyTag003-MBP
UseTagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to N-terminus of MBP via a GSGE…PromoterT7Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF1202
Plasmid#144088PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSETDB1 (SETDB1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMT-Bip-GFP:V5:KDEL
Plasmid#69917PurposeAn endoplasmic reticulum (ER) marker used to visulaize the ER structure in Drosophilia S2 cells.DepositorInsertGFP
UseTagsDrosophila BiP signal sequence, KDEL ER targeting…ExpressionInsectMutationPromoterpMTAvailable sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
Yeast Inducible CRISPRi Library
Pooled Library#161829PurposeATc inducbile yeast CRISPRi libraryDepositorExpressionYeastSpeciesSaccharomyces cerevisiaeUseCRISPRAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-FLT3
Plasmid#23895DepositorInsertFLT3 (FLT3 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pENTR-PIP-FUCCI
Plasmid#118621PurposeGateway cloning vector for the cell cycle indicator PIP-FUCCIDepositorInsertPIP-FUCCI
UseGateway cloningTagsmCherry and mVenusExpressionMutationPromoterAvailable sinceApril 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Nesprin3-C-18
Plasmid#54203PurposeLocalization: Nuclear Membrane , Excitation: 487, Emission: 509DepositorInsertNesprin3 (SYNE3 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-PuroR-SMAR-g5
Plasmid#208377PurposeEpisomal plasmid for expression of 2 proteins with puromycin selection and EGFP monitoringDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterhuman CMVAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-SREBP-2-GFP
Plasmid#204202PurposeMammalian expression of SREBP-2DepositorInsertSREBP-2 (SREBF2 Human)
UseTagsT7x3 and eGFPExpressionMammalianMutationmultiple silent mutationsPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pU6-BbsI-chiRNA
Plasmid#45946PurposePlasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.DepositorInsertU6-BbsI-chiRNA
UseCRISPRTagsExpressionInsectMutationPromoterDm-snRNA:U6:96AbAvailable sinceJune 24, 2013AvailabilityAcademic Institutions and Nonprofits only