295,073 results

1. pXPR_050

   Plasmid

   #96925

   Purpose

   lentiviral expression of gRNA scaffold

   Depositor

   David Root, John Doench

   Article

   Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pXPR_050

   Plasmid

   #96925

   Purpose

   lentiviral expression of gRNA scaffold

   Depositor

   John Doench, David Root

   Article

   Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti PGK Hygro DEST (w530-1)

   Plasmid

   #19066

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Hygro

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti PGK Hygro DEST (w530-1)

   Plasmid

   #19066

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Hygro

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1-Beta4

   Plasmid

   #166773

   Purpose

   Encodes a Gbeta subunit as a "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GNB4 (GNB4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-Beta4

   Plasmid

   #166773

   Purpose

   Encodes a Gbeta subunit as a "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GNB4 (GNB4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-GGamma4-GFP2

   Plasmid

   #166776

   Purpose

   Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma4-GFP2 (GNG4 Human, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains an N-terminal GFP2 and GSAG linker

   Promoter

   CMV

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1-GGamma4-GFP2

   Plasmid

   #166776

   Purpose

   Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma4-GFP2 (GNG4 Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains an N-terminal GFP2 and GSAG linker

   Promoter

   CMV

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEN-luxCDABE

   Plasmid

   #44918

   Depositor

   Harry Mobley

   Article

   Lane et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

   Insert

   sigma 70 promoter
   

   Use

   Luciferase

   Tags

   lux transcriptional fusion

   Expression

   Mutation

   Promoter

   em7

   Available since

   May 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEN-luxCDABE

    Plasmid

    #44918

    Depositor

    Harry Mobley

    Article

    Lane et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

    Insert

    sigma 70 promoter
    

    Use

    Luciferase

    Tags

    lux transcriptional fusion

    Expression

    Mutation

    Promoter

    em7

    Available since

    May 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Hygro-Cas9 donor

    Plasmid

    #86883

    Purpose

    Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus with hygromycin selection

    Depositor

    Danwei Huangfu

    Article

    Shi et al Cell Stem Cell. 2017 Feb 8. pii: S1934-5

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3xFlag, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Hygro-Cas9 donor

    Plasmid

    #86883

    Purpose

    Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus with hygromycin selection

    Depositor

    Danwei Huangfu

    Article

    Shi et al Cell Stem Cell. 2017 Feb 8. pii: S1934-5

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3xFlag, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 22l-COX8-3xFlag-TALE(RNR2-Right)-TadA(8e+V106W)

    Plasmid

    #209296

    Purpose

    mitoBE expression vector, target mtRNR2 gene.

    Depositor

    Wensheng Wei

    Article

    Yi et al Nat Biotechnol. 2023 May 22. doi: 10.103

    Insert

    COX8-3xFlag-TALE(RNR2-Right)-TadA(8e+V106W)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLKO.1 hygro

    Plasmid

    #24150

    Purpose

    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses hygromycin for selection.

    Depositor

    Bob Weinberg

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA

    Available since

    March 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLKO.1 hygro

    Plasmid

    #24150

    Purpose

    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses hygromycin for selection.

    Depositor

    Bob Weinberg

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA

    Available since

    March 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Lenti-LucOS

    Plasmid

    #22777

    Depositor

    Tyler Jacks

    Insert

    OVA-SIY
    

    Use

    Cre/Lox and Lentiviral

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Lenti-LucOS

    Plasmid

    #22777

    Depositor

    Tyler Jacks

    Insert

    OVA-SIY
    

    Use

    Cre/Lox and Lentiviral

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. cyto-iATPSnFR1.0

    Plasmid

    #102550

    Purpose

    cytoplasmic ATP sensor for mammalian cells

    Depositor

    Baljit Khakh

    Article

    Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

    Insert

    iATPSnFR1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. cyto-iATPSnFR1.0

    Plasmid

    #102550

    Purpose

    cytoplasmic ATP sensor for mammalian cells

    Depositor

    Baljit Khakh

    Article

    Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

    Insert

    iATPSnFR1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNH-TrxT

    Plasmid

    #26106

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Opher Gileadi

    Article

    Savitsky et al J Struct Biol. 2010 Jun 10. ():.

    Type

    Empty backbone

    Use

    Tags

    6xHis, TEV cleavage site, and TrxA

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pNH-TrxT

    Plasmid

    #26106

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Opher Gileadi

    Article

    Savitsky et al J Struct Biol. 2010 Jun 10. ():.

    Type

    Empty backbone

    Use

    Tags

    6xHis, TEV cleavage site, and TrxA

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1_ADAR2DD_E488Q

    Plasmid

    #205598

    Purpose

    expresses human ADAR2 deaminase domain (E488Q hyperactive mutant) in mammalian cells

    Depositor

    Marc Friedlander

    Article

    Sekar et al Nat Biotechnol. 2023 Sep 21. doi: 10.103

    Insert

    ADAR2 deaminase domain (ADARB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E488Q hyperactive mutation

    Promoter

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1-GGamma11-GFP2

    Plasmid

    #166780

    Purpose

    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma11-GFP2 (GNG11 Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains an N-terminal GFP2 and GSAG linker

    Promoter

    CMV

    Available since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1-GGamma11-GFP2

    Plasmid

    #166780

    Purpose

    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma11-GFP2 (GNG11 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains an N-terminal GFP2 and GSAG linker

    Promoter

    CMV

    Available since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1-GGamma10-GFP2

    Plasmid

    #166779

    Purpose

    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma10-GFP2 (GNG10 Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains an N-terminal GFP2 and GSAG linker

    Promoter

    CMV

    Available since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1-GGamma10-GFP2

    Plasmid

    #166779

    Purpose

    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma10-GFP2 (GNG10 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains an N-terminal GFP2 and GSAG linker

    Promoter

    CMV

    Available since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pExp-MBP

    Plasmid

    #112568

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and MBP fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-MBP and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pExp-MBP

    Plasmid

    #112568

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and MBP fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-MBP and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCDH-UBC-irfp luc-EF1 Hygro

    Plasmid

    #129438

    Purpose

    express irfp-luc chimera

    Depositor

    Oskar Laur

    Insert

    irfp-luc
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCDH-UBC-irfp luc-EF1 Hygro

    Plasmid

    #129438

    Purpose

    express irfp-luc chimera

    Depositor

    Oskar Laur

    Insert

    irfp-luc
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge