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164,728 results

Showing: 6991 - 7020 of 164728 results

  1. TFORF0058

    Plasmid
    #143007
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MAF (MAF Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143007-plasmid-map-sequence-id-408960

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  2. pCMVβ-p300-myc

    Plasmid
    #30489
    Purpose
    Mammalian expression of human p300 with myc tag
    Depositor
    Tso-Pang Yao
    Insert
    p300 (EP300 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 6, 2011
    Availability
    Academic Institutions and Nonprofits only
    30489-plasmid-map-sequence-id-244762

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  5. pT2/SV-Venus-SIINFEKL

    Plasmid
    #111624
    Purpose
    Sleeping Beauty transposon containing SV-40-driven Venus-LEQLE-SIINFEKL-TEW for antigen presentation studies
    Depositor
    Jon Yewdell
    Insert
    Venus-LEQLE-SIINFEKL-TEW
    Use
    Tags
    LEQLE-SIINFEKL-TEW
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    June 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    111624-plasmid-map-sequence-id-213225

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  6. TRUPATH Triple Gai1

    Plasmid
    #196048
    Purpose
    Encodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphai1-RLuc8 (GNAI1 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196048-plasmid-map-sequence-id-385883

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  8. phCMV-229E-D15

    Plasmid
    #188908
    Purpose
    express truncated 229E spike for pseudovirus production
    Depositor
    David Nemazee
    Insert
    229E spike (S 229E)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    c-terminal 15aa deletion
    Promoter
    CMV
    Available since
    Aug. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    188908-plasmid-map-sequence-id-370665

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  14. HA-Clover-HIF-2alpha Wildtype

    Plasmid
    #163371
    Purpose
    Express HA- and Clover-tagged HIF-2alpha wildtype plasmid
    Depositor
    Violaine Sée
    Insert
    Hypoxia Inducible Factor 2 alpha (EPAS1 Human)
    Use
    Tags
    HA- and Clover-tagged
    Expression
    Mammalian
    Mutation
    Wildtype
    Promoter
    CMV Promoter
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    163371-plasmid-map-sequence-id-322295

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  15. pGNW2

    Plasmid
    #122086
    Purpose
    suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells
    Depositor
    Pablo Ivan Nikel
    Article
    Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    122086-plasmid-map-sequence-id-451911

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  16. p406TEF1

    Plasmid
    #15976
    Depositor
    Fred Cross, Nicolas Buchler
    Insert
    TEF1 promoter + polylinker + CYC1 terminator (TEF1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    TEF1 promoter is between SacI and XbaI restrictio…
    Promoter
    Available since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15976-plasmid-map-sequence-id-8461

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  17. pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] (AAV8)

    Viral Prep
    #84446-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] (#84446). In addition to the viral particles, you will also receive purified pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] plasmid DNA. CAG-driven, Cre-dependent expression of Jaws-KGC-tdTomato-ER2 for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Article
    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi
    Promoter
    CAG
    Tags
    None
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only

  22. BRD4

    Plasmid
    #38942
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BRD4 (BRD4 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    38942-plasmid-map-sequence-id-348518

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  26. pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA

    Plasmid
    #85756
    Purpose
    All in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).
    Depositor
    Lawrence Stanton
    Article
    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:
    Inserts
    Csy4-T2A-SpRFN-P2A-GFP
    gRNA
    Use
    CRISPR
    Tags
    Csy4, Csy4 recognition sequence, FokI fusion via …
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CMV and U6
    Available since
    Jan. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85756-plasmid-map-sequence-id-170275

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  27. pKK-BiFC-Venus

    Plasmid
    #105804
    Purpose
    Concomitant expression of two proteins in fusion with fragments of Venus protein. Protein interactions studies by bimolecular fluorescence complementation (BiFC), including BiFC-FRET assays.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    VN173 (I152L); VC155
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105804-plasmid-map-sequence-id-203400

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  30. pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE (AAV1)

    Viral Prep
    #104495-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE (#104495). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE plasmid DNA. CAG-driven, Cre-dependent GCaMP7s calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project, Douglas Kim
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Promoter
    CAG
    Tags
    None
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 6991 - 7020 of 164728 results