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Showing: 6991 - 7020 of 160936 results

  5. pAcBac1.tR4-MbPyl

    Plasmid
    #50832
    Purpose
    baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells
    Depositor
    Peter Schultz
    Article
    Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11
    Inserts
    two-copy Pyl tRNA cassette
    MbPyl
    two copy Pyl tRNA cassette
    Use
    Baculoviral
    Tags
    Expression
    Insect
    Mutation
    Promoter
    CMV and U6 and H1
    Available since
    Feb. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    50832-plasmid-map-sequence-id-331231

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  7. AAV.CamKII.GCaMP6s.WPRE.SV40

    Plasmid
    #107790
    Purpose
    AAV expression of ultrasensitive protein calcium sensor from the CamKII promoter
    Depositor
    James M. Wilson
    Has Service
    AAV9
    Insert
    GCaMP6s
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CamKII
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107790-plasmid-map-sequence-id-207334

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  16. ITGB3

    Plasmid
    #51921
    Purpose
    Expresses full-length Integrin beta-3 precursor ectodomain in mammalian cells. Stop codon before C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    ITGB3 (ITGB3 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Stop Codon at amino acid 718, before CD4, bio and…
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51921-plasmid-map-sequence-id-79900

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  17. SpyTag003-MBP

    Plasmid
    #133450
    Purpose
    Expresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasm
    Depositor
    Mark Howarth
    Article
    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p
    Insert
    SpyTag003-MBP
    Use
    Tags
    His6 and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Peptide tag added to N-terminus of MBP via a GSGE…
    Promoter
    T7
    Available since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    133450-plasmid-map-sequence-id-267039

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  19. mCherry-PCNA-19-SV40NLS-4

    Plasmid
    #55117
    Purpose
    Localization: Nucleus, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  23. pLKO.1-puro U6 sgRNA BfuAI large stuffer

    Plasmid
    #52628
    Purpose
    lentiviral U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites, improved cassette cloning efficiency
    Depositor
    Scot Wolfe
    Insert
    Kanamycin cassette
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    April 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    52628-plasmid-map-sequence-id-217983

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  25. pSelAct-KO

    Plasmid
    #174374
    Purpose
    Plasmid for unmarked deletions, pUC19 backbone, Apr resistant casette, codA::app counterselection casette, gateway compatible
    Depositor
    Gitta Coaker
    Article
    Stevens et al Mol Plant Microbe Interact. 2021 Dec;34(
    Type
    Empty backbone
    Use
    Vector for 2-step knockout by homologous recombin…
    Tags
    Expression
    Mutation
    Promoter
    N/A
    Available since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    174374-plasmid-map-sequence-id-356074

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  26. ShuttleNSE

    Plasmid
    #50958
    Purpose
    A backbone for adenovirus creation by the AdEasy method, similar to ShuttleCMV (Addgene Plasmid # 16403), except it uses a Neuron-specific enolase (NSE) promoter instead of the CMV promoter
    Depositor
    James Bamburg
    Article
    Mi et al PLoS One. 2013 Dec 31;8(12):e83609. doi:
    Type
    Empty backbone
    Use
    Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    NSE
    Available since
    Feb. 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    50958-plasmid-map-sequence-id-76714

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  30. pFS_0414_pET30_MCS-BBaJ23100-LuxR-B0015term-pLux rR-Fluc-rrnBterm-pCat-tetR-Term-ptetA-Rluc-rrnBterm_FsRT-Cas1-Cas2_ARRAY2_FaqI

    Plasmid
    #117005
    Purpose
    Expresses FsRT-Cas1-Cas2 under pT7lac, Fluc under pLuxR and Rluc under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout
    Depositor
    Randall Platt
    Article
    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:
    Insert
    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    117005-plasmid-map-sequence-id-227039

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Showing: 6991 - 7020 of 160936 results