167,910 results

1. pmCherry-MYRIP

   Plasmid

   #89586

   Purpose

   Expression plasmid for a positive control prey protein in NanoSPD 2.0 assays.

   Depositor

   Thomas Friedman

   Article

   Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

   Insert

   MYRIP (Myrip Mouse)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKm

   Plasmid

   #170331

   Purpose

   backbone vector for clone construction

   Depositor

   Deqiang Duanmu

   Article

   Wang et al Front Microbiol. 2021 Jun 18;12:686008.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. 3XFlagNICD2

   Plasmid

   #20184

   Depositor

   Raphael Kopan

   Article

   Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

   Insert

   Notch 2 intracellular domain (Notch2 Mouse)
   

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   Contains murine Notch 2 Intracellular Domain (Met…

   Promoter

   CMV

   Available Since

   Jan. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. mEmerald-Talin-C-18

   Plasmid

   #62763

   Purpose

   Localization: Talin/Focal Adhesions, C1 Cloning Vector, Excitation:487, Emission:509

   Depositor

   Michael Davidson

   Insert

   Talin
   

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   March 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. FUW-tetO-NFIA

   Plasmid

   #141403

   Purpose

   Inducible Expression of NFIA

   Depositor

   Lorenz Studer

   Article

   Tchieu et al Nat Biotechnol. 2019 Mar;37(3):267-275.

   Insert

   NFIA (NFIA Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Slug_pGL2

   Plasmid

   #31695

   Depositor

   Paul Wade

   Article

   Fujita et al Cell. 2003 Apr 18. 113(2):207-19.

   Insert

   Slug (SNAI2 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. FUGW-APPIce-T2A-mCherry

   Plasmid

   #190806

   Purpose

   Express Icelandic mutant APP from Ubiquitin C promoter, co-expressing mCherry

   Depositor

   Ronald Hart

   Insert

   APP (APP Human)
   

   Use

   Lentiviral

   Mutation

   Icelandic (A673T)

   Promoter

   Ubi C

   Available Since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-MYO10-HMM-Nanotrap

   Plasmid

   #87255

   Purpose

   Expression plasmid to perform NanoSPD 2.0 assays in mammalian cells.

   Depositor

   Thomas Friedman

   Article

   Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

   Insert

   MYO10 (Myo10 Mouse)
   

   Tags

   GFP-nanotrap

   Expression

   Mammalian

   Mutation

   Myo10 is truncated to contain aa.1-941

   Promoter

   CMV

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBABEpuro-ERBB2 A775_G776insV, G776C

   Plasmid

   #40979

   Depositor

   Matthew Meyerson

   Article

   Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   A775_G776insV, G776C

   Available Since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

10. P532_POU4F2-p2A-tdTomato-p2A-Thy1.2

    Plasmid

    #239102

    Purpose

    Human donor plasmid for integration of p2A-h2b-EGFP into the 3' end of POU4F2 (aka BRN3B). This includes the donor casette and a U6 driven guide sequence to be used with Cas9.

    Depositor

    Karl Wahlin

    Article

    Wahlin et al Front Cell Dev Biol. 2021 Nov 16;9:76472

    Insert

    POU4F2 (POU4F2 Human)
    

    Use

    Donor plasmid for integration into the human geno…

    Tags

    p2A-tdTomato-p2A-Thy1.2

    Expression

    Mammalian

    Promoter

    Endogenous POU4F2

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFBOH-MHL_EIF3B:127-814

    Plasmid

    #210909

    Purpose

    Insect expression of human EIF3B fragment D127 to E814. N-terminal 6X His tag with TEV cleavage.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    EIF3B:D127-E814 (EIF3B Human)
    

    Tags

    MHHHHHHSSGRENLYFQGMQ

    Expression

    Insect

    Mutation

    Y760C sequence conflict

    Promoter

    Polyhedrin

    Available Since

    March 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pcDNA3.1_PAsfCherry2(1-10)

    Plasmid

    #82612

    Purpose

    Express PAsfCherry2(1-10) in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    PAsfCherry2(1-10)
    

    Expression

    Mammalian

    Mutation

    changed Glu 118 to Gln, changed Thr 128 to Ile

    Promoter

    CMV

    Available Since

    Jan. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. CMV-LgBit

    Plasmid

    #234738

    Purpose

    Mammalian expression of large NanoBit fragment (LgBit)

    Depositor

    Nevin Lambert

    Article

    Jang et al Elife. 2024 Nov 8;13:RP97033. doi: 10.75

    Insert

    LgBit
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSFFV_sfCherry2_TagBFP

    Plasmid

    #83031

    Purpose

    Expresses full length sfCherry2 and TagBFP fusion in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    sfCherry+full length_TagBFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    3 mutations in sfCherry full length: E118Q, T128I…

    Promoter

    SFFV

    Available Since

    Jan. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. FUGW-3xHA-KCNJ6-mCherry

    Plasmid

    #197032

    Purpose

    Express 3xHA-tagged human KCNJ6 (Girk2), co-expressed with mCherry, from UbiC promoter

    Depositor

    Ronald Hart

    Insert

    KCNJ6 (KCNJ6 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAurora

    Plasmid

    #190158

    Purpose

    Regulates bacterial expression of target gene in blue-light-activated manner. Based on Nakamurella multipartita PAL.

    Depositor

    Andreas Moeglich

    Article

    Ranzani et al ACS Synth Biol. 2022 Oct 21;11(10):3482-

    Type

    Empty backbone

    Tags

    His6 tag

    Expression

    Bacterial

    Available Since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWZL Neo Myr Flag MAST1

    Plasmid

    #20532

    Depositor

    Jean Zhao, William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    MAST1 (MAST1 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Available Since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pD649-HAsp-CEACAM5-COMP5AP-AviTag-9xHis

    Plasmid

    #157501

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    CEACAM5 (CEACAM5 Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBMN-YFP-Parkin

    Plasmid

    #59416

    Purpose

    to make YFP-Parkin stable cell line with retrovirus

    Depositor

    Richard Youle

    Article

    Yamano et al Elife (Cambridge). 2014 Feb 25;3:e01612.

    Insert

    YFP-Parkin (PRKN Human)
    

    Use

    Retroviral

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    Aug. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. TetO-FUW-NfiB

    Plasmid

    #64900

    Purpose

    Inducible expression of murine CDS encoding for NfiB. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiA allows reprograming into iAstro. cells.

    Depositor

    Vania Broccoli

    Article

    Caiazzo et al Stem Cell Reports. 2015 Jan 13;4(1):25-3

    Insert

    NfiB (Nfib Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TetO

    Available Since

    July 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. pD649-HAsp-EDA2R-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156531

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    EDA2R (EDA2R Human)
    

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pD649-HAsp-EDAR-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156554

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    EDAR (EDAR Human)
    

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    Aug. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. mVenus-NES-SspB-MCS-P2A-iLIDcaax

    Plasmid

    #173864

    Purpose

    Mammalian expression of mVenus-NES-SspB-P2A-iLIDcaax (opto-control) containing multiple cloning sites (MCS)

    Depositor

    Takao Nakata

    Article

    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

    Insert

    mVenus-NES-SspB-MCS-P2A-iLIDcaax
    

    Tags

    C-terminal CAAX-box and mVenus

    Expression

    Mammalian

    Available Since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. CAV1-mCherry

    Plasmid

    #27705

    Depositor

    Ari Helenius

    Article

    Hayer et al Traffic. 2010 Mar . 11(3):361-82.

    Insert

    caveolin-1 (CAV1 Canis lupus familiaris)
    

    Tags

    monomeric Cherry

    Expression

    Mammalian

    Available Since

    July 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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25. MAC_C_INSR

    Plasmid

    #187788

    Purpose

    MAC-tagged gene expression of human INSR

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    INSR (INSR Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCMV mClover3-Galectin-3

    Plasmid

    #215376

    Purpose

    Expression of the endolysosomal damage marker Galectin-3 with an N-terminal mClover3 tag.

    Depositor

    Franz-Ulrich Hartl

    Article

    Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

    Insert

    LGALS3 (LGALS3 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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27. KCNJ6_pCSdest

    Plasmid

    #53804

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    KCNJ6 (KCNJ6 Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

28. pcDNA3-AKAR4

    Plasmid

    #61619

    Purpose

    FRET-based biosensor for monitoring PKA activity

    Depositor

    Jin Zhang

    Article

    Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

    Insert

    AKAR4
    

    Tags

    Cerulean and cpVenus[E172]

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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29. pET31b-T7-Spinach2-Dp

    Plasmid

    #79159

    Purpose

    Expresses the Dp biosensor under a T7 promoter

    Depositor

    Ming Hammond

    Article

    Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

    Insert

    T7-tRNA-Spinach2-Dp
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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30. pcDNA-dCas9-p300 Core (D1399Y)

    Plasmid

    #61358

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9; D1399Y mutation i…

    Promoter

    CMV

    Available Since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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