164,438 results
-
Plasmid#154264PurposeDox/Tet-inducible expression of dominant negative CREB (A-CREB) in mammalian cells.DepositorInsertdominant negative CREB
UseTagsExpressionMammalianMutationPromoterTight/tetOAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag Runx1 FL (P#1790)
Plasmid#14585DepositorInsertRunx1 (Runx1 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
Desmoglein2-mCherry
Plasmid#36991DepositorInsertDsg2 (DSG2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pVSV eGFP RABV-G
Plasmid#31833DepositorInsertVSV genome
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLenti_P2A_Puro
Plasmid#211364PurposeLentiviral expression vector for an insert of interest linked to a P2A-T2A puromycin sequence. Produces virus very efficiently. Can also be used for regular transfectionsDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
AKAP1-hLOV-TEVcs-GAL4bd-VP64-V5
Plasmid#170995PurposeHiLITR transcription factor with AKAP1 N-terminal tmd targeting sequence (mitochondria)DepositorInsertAKAP1(tmd)-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4bd-VP64-V5 (AKAP1 Synthetic)
UseLentiviral and Synthetic BiologyTagsV5ExpressionMammalianMutationPromoterEf1-alphaAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
CRY2-mCherry-Miro1TM
Plasmid#102247PurposeExpresses fusion of CRY2PHR with mCherry and transmembrane domain of Miro1DepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ERBB4
Plasmid#23875DepositorInsertERBB4 (ERBB4 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
TFORF2886
Plasmid#143655PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGLI3 (GLI3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRZeroT
Plasmid#120276PurposeBifunctional vector for TA-cloning and blunt-end cloning of PCR-products using ZERO-backgound systemDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
pCherry.90 alpha
Plasmid#108222PurposeMammalian expression vector for mCherry fusion to human Hsp90 alphaDepositorInsertmCherry-Hsp90a (HSP90AA1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV/TO Hygro DEST (693-2)
Plasmid#17291Purpose3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, HygroDepositorTypeEmpty backboneUseLentiviral; Destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_HaloTag7_T2A_EGFP
Plasmid#169325PurposeExpression of HaloTag7 in mammalian cellsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS-kI
Plasmid#51553PurposeMammalian phiC31 integrase expression vector. For use in applications where pseudosite integration is not desired.DepositorInsertphiC31 integrase
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEC3135 (pERASE)
Plasmid#220306PurposeEnables scarless gene deletions in Streptococcus pyogenes using the counterselection marker pheS*DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV GFP
Plasmid#49055Purposeadeno-associated virus (AAV) encoding a green fluorescent proteinDepositorInserteGFP
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
ACE2
Plasmid#164219PurposeExpresses full length human ACE2 protein, along with TagBFP reporter and Puromycin selection markerDepositorInsertACE2 (ACE2 Human)
UseLentiviralTagsExpressionMutationsilent mutation to remove internal EcoRI site (GA…PromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSL1425 (pSPIN, pCDF backbone)
Plasmid#160735PurposeSingle-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA and has BsaI sites for spacer cloning. pCDF backbone.DepositorInsertVchCAST proteins, crRNA, and donor DNA
UseTagsExpressionBacterialMutationPromoterJ23119Available sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pP35S (GB0030)
Plasmid#68163PurposeProvides the CaMV 35S Promoter as a level 0 GoldenBraid partDepositorInsertCaMV 35S promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBT374
Plasmid#125615PurposeExpresses evoFERNY-BE4max-NG in mammalian cellsDepositorInsertevoFERNY-BE4max-NG
UseTagsExpressionMammalianMutationH102P D104NPromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-AR Q48
Plasmid#86428PurposeFluorescent human androgen receptor (fused to EGFP) with expanded polyglutamine regionDepositorInsertAndrogen receptor (AR) (AR Human)
UseTagsEGFPExpressionMammalianMutationPoly-glutamine expansion, G130DPromoterCMVAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mDlx-GFP-Fishell-1 (AAV Retrograde)
Viral Prep#83900-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-mDlx-GFP-Fishell-1 (#83900). In addition to the viral particles, you will also receive purified pAAV-mDlx-GFP-Fishell-1 plasmid DNA. mDlx-driven expression of GFP in GABA-ergic interneurons. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromotermDlxTagsGFPAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_ETV6_NTRK3
Plasmid#205817PurposeExpress mEGFP-tagged fusion protein, ETV6_NTRK3 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRE-mClover3-TDP-43ΔNLS
Plasmid#214917Purposeinducible expression of TDP-43 harboring mutant NLS and N-terminal mClover3. Expression yields cytosolic TDP-43 that forms peinuclear punctaDepositorInsertTDP-43ΔNLS (TARDBP Human)
UseLentiviralTagsmClover3ExpressionMutationmutant NLS GCAGCAGCAATGGATGAGACAGATGCTTCATCAGCAGT…PromoterAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-N-RAM-d2tTA-TRE-mKate2
Plasmid#140275PurposeN-RAM, the Npas4-dependent reporterDepositorInsertmKate2
UseAAVTagsExpressionMutationPromoterTREAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.GCaMP6s.WPRE.SV40 (AAV5)
Viral Prep#100842-AAV5PurposeReady-to-use AAV5 particles produced from pAAV.CAG.Flex.GCaMP6s.WPRE.SV40 (#100842). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.GCaMP6s.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsNoneAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLM-mCerulean-cMyc
Plasmid#23244DepositorInsertmCerulean-2A-MYC (MYC synthetic fluorescent protein, Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiPVe3_dTomato_nlsdTomato
Plasmid#213940PurposeAAV construct expressing dTomato driven by PV+ basket cell-targeting enhancer. Alias: pAAV_WP0003_dTomato_nlsdTomatoDepositorHas ServiceAAV PHP.eBInsertdTomato_P2A_nls.dTomato
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIB184 (EM)
Plasmid#90194PurposeE. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoterDepositorTypeEmpty backboneUseTagsNoneExpressionBacterialMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-fDIO-GFP-IRES-tTA
Plasmid#118026PurposeAAV vector for sparse and bright labeling in a cell-type specific mannerDepositorInsertTRE-fDIO-GFP-IRES-tTA
UseAAVTagsExpressionMutationSee depositor comments belowPromoterTREAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB5321
Plasmid#204831PurposeAn integrating plasmid containing both the inducible enotetSW3 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.DepositorInsertstetR
mECitrine
UseTagsExpressionYeastMutationPromoterCMV promoter and enotetSW3 promoterAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET16b-Trx
Plasmid#36944DepositorInsertThioredoxin 1 (TXN Human)
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceMay 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
m168
Plasmid#34886DepositorInsertSindbis virus envelope protein
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Xist-PA
Plasmid#26760DepositorInsertXist (Xist Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
p3HR2
Plasmid#220738PurposeComponent of yeast three hybrid systemDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-HDAC2
Plasmid#222553PurposePiggyBac transposon plasmid for doxycycline inducible expression of HDAC2DepositorInsertHDAC2 (HDAC2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
acGFP NES visual barcode
Plasmid#158673Purposetagging clonal populations with NES acGFP fluorescent protein. Adding NES to acGFP WCDepositorInsertacGFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Ii-OVA.II
Plasmid#64597PurposeExpression of invariant chain fused to chicken ovalbumin 315-353 amino acids as model antigen for study of MHC class II presentationDepositorInsertOvalbumin
UseTagsInvariant chainExpressionMammalianMutationOnly amino acids 315-353 (class II epitopes)PromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
-
EFNB1-bio-His
Plasmid#51749PurposeExpresses full-length Ephrin-B1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertEFNB1 (EFNB1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-RFP
Plasmid#22907DepositorInserthuman Synapsin I promoter driving DsRedExpress (SYN1 Human)
UseAAV; Adeno-associated virusTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_eCBmut-IRES-mCherry-CAAX
Plasmid#164612PurposeExpress the endocannabinoid non-responsive sensor GRAB_eCB2.0 in mammalian cellsDepositorInsertEndocannabinoid non-responsive sensor GRAB_eCBmut
UseTagsExpressionMammalianMutationS383T, F177APromoterCMVAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV-EF1a-mCherryLuciferase-W
Plasmid#200101PurposeLentiviral vector expressing mCherry fused with Luciferase at the C-terminusDepositorInsertLuciferase
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHCMV-SS-antiHAscFv-TM
Plasmid#198302PurposeDisplays anti-HA scFv from the surface of mammalian cellsDepositorInsertpHCMV-SS-antiHAscFv-TM
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
HTR2B-Tango
Plasmid#66410PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertHTR2B (HTR2B Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR_DKO
Plasmid#183192PurposePlasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screenDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6, mU6Available sinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRPF185
Plasmid#106367PurposeC. difficile inducible expression system. Shuttle plasmid containing a tetracycline-inducible gusADepositorInsertTerm(fdx)-Ptet-gusA-Term(slpA) (gusA )
UseE. coli - c. difficile shuttle vectorTagsExpressionMutationcodon optimised for C. difficilePromoterPtetAvailable sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Opa1
Plasmid#191963PurposeExpression of OPA1.DepositorInsertOpa1 (Opa1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only