Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 32,594 results for: LIS;

Showing: 9641 - 9680 of 32594 results

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pCDNA5-TEX264-SIM1_mut-cGFP

Plasmid

#220212

Purpose

Mammalian expression of TEX264 with SIM1 motif mutated, fused at C-terminus to GFP

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Tags

GFP

Expression

Mammalian

Mutation

p.111LIDLY>AAAAA; c.331C>G,332T>C,334A&g…

Promoter

CMV/TetO

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CASI-InteinC-SpRY C aa714-1368-U6-Camk2d sgRNA

Plasmid

#209788

Purpose

Expresses SpRY cas9C by the constitutive CASI promoter and sgRNA targeting the oxidative activation sites of Camk2d by U6 promoter

Depositor

Yuxuan Guo

Article

Yang et al Circulation. 2024 May 28;149(22):1778-17

Insert

SpRY C, Camk2d sgRNA

Use

AAV

Promoter

CASI

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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LacI-pLacI-pI pm

Plasmid

#218098

Purpose

Phagemid that expresses LacI, and pI under the Lac promoter

Depositor

Kevin Esvelt

Article

Gretton et al bioRxiv 2024.03.20.585782

Insert

pI

Expression

Bacterial

Promoter

Lac promoter

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-BCL9

Plasmid

#202662

Purpose

Tet-ON 3rd generation expression vector with inducible expression of BCL9 and a puromycin selection cassette

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

BCL9 transcription coactivator (BCL9 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-Hyg-CMV-BCL9

Plasmid

#202667

Purpose

BCL9 overexpression vector with hygromycin resistance

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

BCL9 transcription coactivator (BCL9 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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Amber-free HIV-1 deltaRT

Plasmid

#213007

Purpose

An replication-incompetent amber-free HIV-1 encodes full-length Env BG505 with RT-deleted Q23 as the backbone.

Depositor

Maolin Lu

Article

Ao et al Cell Chem Biol. 2024 Jan 8:S2451-9456(23

Inserts

HIV-1 Q23 ΔRT backbone (reverse transcriptase is deleted)
Env BG505

Use

Unspecified

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-858_CD19-BBz_CAR_TFAP4

Plasmid

#207503

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

TFAP4, CD19-BBz_CAR (TFAP4 Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_PICK1_POLR2F

Plasmid

#205894

Purpose

Express mEGFP-tagged fusion protein, PICK1_POLR2F from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

PICK1_POLR2F (PICK1, POLR2F Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_MLLT10_PICALM

Plasmid

#205858

Purpose

Express mEGFP-tagged fusion protein, MLLT10_PICALM from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

MLLT10_PICALM (MLLT10, PICALM Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_WASL_VPS13D

Plasmid

#205953

Purpose

Express mEGFP-tagged fusion protein, WASL_VPS13D from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

WASL_VPS13D (WASL, VPS13D Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_TSG101_YWHAB

Plasmid

#205948

Purpose

Express mEGFP-tagged fusion protein, TSG101_YWHAB from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

TSG101_YWHAB (TSG101, YWHAB Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_RBM28_PRRT4

Plasmid

#205909

Purpose

Express mEGFP-tagged fusion protein, RBM28_PRRT4 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

RBM28_PRRT4 (RBM28, PRRT4 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_RPRD1A_SLC39A6

Plasmid

#205912

Purpose

Express mEGFP-tagged fusion protein, RPRD1A_SLC39A6 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

RPRD1A_SLC39A6 (RPRD1A, SLC39A6 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_SAFB2_SAFB

Plasmid

#205917

Purpose

Express mEGFP-tagged fusion protein, SAFB2_SAFB from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

SAFB2_SAFB (SAFB2, SAFB Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_SLC16A14_SP110

Plasmid

#205921

Purpose

Express mEGFP-tagged fusion protein, SLC16A14_SP110 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

SLC16A14_SP110 (SLC16A14, SP110 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_PAX5_ZCCHC7

Plasmid

#205888

Purpose

Express mEGFP-tagged fusion protein, PAX5_ZCCHC7 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

PAX5_ZCCHC7 (PAX5, ZCCHC7 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_PAX5_ZNF276

Plasmid

#205889

Purpose

Express mEGFP-tagged fusion protein, PAX5_ZNF276 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

PAX5_ZNF276 (PAX5, ZNF276 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_PAX5_ZNF521

Plasmid

#205890

Purpose

Express mEGFP-tagged fusion protein, PAX5_ZNF521 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

PAX5_ZNF521 (PAX5, ZNF521 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_PLEKHA5_XRCC6BP1

Plasmid

#205896

Purpose

Express mEGFP-tagged fusion protein, PLEKHA5_XRCC6BP1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

PLEKHA5_XRCC6BP1 (PLEKHA5, XRCC6BP1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_PPFIA1_PAPD5

Plasmid

#205898

Purpose

Express mEGFP-tagged fusion protein, PPFIA1_PAPD5 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

PPFIA1_PAPD5 (PPFIA1, TENT4B Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
CL20_mEGFP_PSD3_NTN1

Plasmid

#205902

Purpose

Express mEGFP-tagged fusion protein, PSD3_NTN1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

PSD3_NTN1 (PSD3, NTN1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
CL20_mEGFP_RANBP3_SLC25A23

Plasmid

#205907

Purpose

Express mEGFP-tagged fusion protein, RANBP3_SLC25A23 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

RANBP3_SLC25A23 (RANBP3, SLC25A23 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
CL20_mEGFP_OPCML_NTM

Plasmid

#205874

Purpose

Express mEGFP-tagged fusion protein, OPCML_NTM from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

OPCML_NTM (OPCML, NTM Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_MSMP_RGP1

Plasmid

#205860

Purpose

Express mEGFP-tagged fusion protein, MSMP_RGP1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

MSMP_RGP1 (MSMP, RGP1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_KLK3_KLK2

Plasmid

#205839

Purpose

Express mEGFP-tagged fusion protein, KLK3_KLK2 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

KLK3_KLK2 (KLK3, KLK2 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_BTN3A3_BTN3A1

Plasmid

#205784

Purpose

Express mEGFP-tagged fusion protein, BTN3A3_BTN3A1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

BTN3A3_BTN3A1 (BTN3A3, BTN3A1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-6P-1 AUP1 61-410

Plasmid

#185334

Purpose

Bacterial expression of AUP1 with GST fusion protein for binding assays

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 61-610 only

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-6P-1-gp78 RING

Plasmid

#185353

Purpose

RING domain-containing GST fusion protein for ubiquitination assays.

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

gp78 cytoplasmic domain (AMFR Human)

Tags

GST

Expression

Bacterial

Mutation

Stop codon after aa 393

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
anti-Calbindin scFv [L109/57] with sortase tag

Plasmid

#204421

Purpose

Mammalian Expression of anti-Calbindin scFv with a sortase tag for direct dye conjugation. Derived from hybridoma L109/57.

Depositor

Jeff Lichtman

Article

Han et al Nat Commun. 2024 Aug 5;15(1):6648. doi:

Insert

Recombinant mouse scFv targeting Calbindin (Homo sapiens)

Tags

6xHis tag and Sortase tag

Expression

Mammalian

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
anti-GFAP R416WT scFv [N206B/9] with sortase tag

Plasmid

#204420

Purpose

Mammalian Expression of anti-GFAP scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N206B/9.

Depositor

Jeff Lichtman

Article

Han et al Nat Commun. 2024 Aug 5;15(1):6648. doi:

Insert

Recombinant mouse scFv targeting GFAP R416WT (Homo sapiens)

Tags

6xHis tag and Sortase tag

Expression

Mammalian

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
anti-Parvalbumin scFv [L114/81] with sortase tag

Plasmid

#204423

Purpose

Mammalian Expression of anti-Parvalbumin scFv with a sortase tag for direct dye conjugation. Derived from hybridoma L114/81.

Depositor

Jeff Lichtman

Article

Han et al Nat Commun. 2024 Aug 5;15(1):6648. doi:

Insert

Recombinant mouse scFv targeting Parvalbumin (Rattus norvegicus)

Tags

6xHis tag and Sortase tag

Expression

Mammalian

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
anti-Kv1.2 scFv [K14/16] with sortase tag

Plasmid

#204425

Purpose

Mammalian Expression of anti-Kv1.2 scFv with a sortase tag for direct dye conjugation. Derived from hybridoma K14/16.

Depositor

Jeff Lichtman

Article

Han et al Nat Commun. 2024 Aug 5;15(1):6648. doi:

Insert

Recombinant mouse scFv targeting Kv1.2 (Homo sapiens)

Tags

6xHis tag and Sortase tag

Expression

Mammalian

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_023d-sgCiPOLR2D

Plasmid

#202445

Purpose

Expression of a CRISPRi guide targeting POLR2D

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

POLR2D gRNA (POLR2D Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-6P-1-gp78 429-611

Plasmid

#185354

Purpose

GST fusion protein for quantification of gp78 levels

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

gp78 (AMFR Human)

Tags

GST

Expression

Bacterial

Mutation

aa 429-611 only

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-6P-1 AUP1 292-410 5E

Plasmid

#185332

Purpose

Bacterial expression of AUP1 with GST fusion protein for binding assays

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 292-410 only, with mutations R382E/Q383E/K390E…

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-6P-1 AUP1 292-410 A397D

Plasmid

#185333

Purpose

Bacterial expression of AUP1 with GST fusion protein for binding assays

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 292-410 only, with mutation A379D

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_003-sgUBR4

Plasmid

#202442

Purpose

Expression of a guide RNA targeting UBR4

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

UBR4 gRNA (UBR4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_003-sgUBA6

Plasmid

#202440

Purpose

Expression of a guide RNA targeting UBA6

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

UBA6 gRNA (UBA6 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Tbg-CES2C-ΔC-S230A

Plasmid

#200561

Purpose

Secreted mouse CES2C S230A mutant driven by heptaocyte-specific promoter in AAV viral vector

Depositor

Jonathan Long

Article

Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

Insert

Mouse Carboxylesterase 2C S230A mutant delta C-terminal (Ces2c Mouse)

Use

AAV

Tags

Flag

Promoter

TBG promoter

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C1-G2BRgp78

Plasmid

#185344

Purpose

Expression of soluble G2BR, amino acids 575-600 of human gp78

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

gp78 aa 574-600 (G2BR) (AMFR Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa 575-600 only

Available Since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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