167,913 results

1. pLG006 (Ec67 retron)

   Plasmid

   #157884

   Purpose

   Expresses the Ec67 retron defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   Ec67 retron
   

   Expression

   Bacterial

   Available Since

   Dec. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pR26 CAG AsiSI/MluI

   Plasmid

   #74286

   Purpose

   Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter and loxP flanked stop cassette, for cloning into AsiSI or Mlui

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Rosa26 5-homology region
   

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-hSyn-Kir2.1WT-2A-mScarlet-KASH

   Plasmid

   #203840

   Purpose

   Kir2.1 expression for silencing neurons and mScarlet-KASH expression

   Depositor

   Ivo Spiegel

   Article

   Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

   Has Service

   AAV8

   Insert

   Kir2.1 (Kcnj2 Mouse)
   

   Use

   AAV

   Tags

   2A-mScarlet-KASH

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFOK

   Plasmid

   #166651

   Purpose

   Suicide vector for gene manipulation

   Depositor

   Dirk Bumann

   Article

   Cianfanelli et al BMC Microbiol. 2020 May 24;20(1):129. do

   Insert

   I-sceI, sacB
   

   Use

   Synthetic Biology

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. peYFP-C1-mTOR

   Plasmid

   #73384

   Purpose

   Expresses YFP tagged mTOR

   Depositor

   Taekjip Ha, Jie Chen

   Article

   Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

   Insert

   mTOR (MTOR Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AAV-hSyn-ASAP4e-Kv-WPRE

   Plasmid

   #201031

   Purpose

   Expresses somatically enriched ASAP4e in neurons, can be used to package AAV

   Depositor

   Michael Lin

   Article

   Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

   Insert

   ASAP4e-Kv
   

   Use

   AAV

   Available Since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CMV-GFP-NMHC II-A

   Plasmid

   #11347

   Depositor

   Robert Adelstein

   Article

   Wei et al Mol Biol Cell. 2000 Oct . 11(10):3617-27

   Insert

   NMHC II-A (MYH9 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1-Peredox-mCherry

   Plasmid

   #32383

   Depositor

   Gary Yellen

   Article

   Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

   Insert

   Peredox-mCherry
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBABE-neo largeTcDNA

   Plasmid

   #1780

   Purpose

   Retroviral expression of Large T Antigen. Used to create immortalized cell lines.

   Depositor

   Bob Weinberg

   Article

   Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

   Insert

   large T antigen (SV40gp6 simian virus)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   June 3, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pT7-EGFP-C1-HsEDC4_S

    Plasmid

    #147455

    Purpose

    Mammalian Expression of HsEDC4. Please note that this plasmid does not contain the T7 promoter.

    Depositor

    Elisa Izaurralde

    Article

    Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

    Insert

    HsEDC4 (EDC4 Human)
    

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti CMV Neo DEST (705-1)

    Plasmid

    #17392

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Neo

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Destination vector

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAVS1-tet-iCas9-BFP2

    Plasmid

    #125519

    Purpose

    all-in-one-inducible Cas9

    Depositor

    Jason Moffat

    Article

    Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.

    Insert

    AAVS1-TET-ON 3G-SpCas9-P2A-BFP2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUC18R6K-mini-Tn7T

    Plasmid

    #64958

    Purpose

    mini-Tn7 base vector with transcriptional terminator

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    July 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pLX311-Cas9

    Plasmid

    #118018

    Purpose

    Constitutive expression of Cas9

    Depositor

    William Hahn, David Root

    Article

    Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

    Insert

    Cas9
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMK1253

    Plasmid

    #133058

    Purpose

    Lentiviral vector with EF1A promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-Clover2 fusion protein.

    Depositor

    Martin Kampmann

    Article

    Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

    Insert

    Tau.K18(LM)
    

    Use

    Lentiviral

    Tags

    mClover2

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pVDL9.3

    Plasmid

    #168299

    Purpose

    Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA

    Depositor

    Víctor de Lorenzo

    Article

    Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do

    Insert

    Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD
    

    Expression

    Bacterial

    Promoter

    LacI-Plac

    Available Since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMEH303 NLS dTomato 6x high affinity MS2 loops

    Plasmid

    #168215

    Purpose

    Mammalian expression of nuclear-localized dTomato with 6 high affinity MS2 loops

    Depositor

    Joshua Leonard

    Article

    Hung et al J Extracell Vesicles. 2016 May 13;5:3102

    Insert

    NLS dTomato
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-FLAG-MTOR-R2505P

    Plasmid

    #69015

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-R2505P (MTOR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Arginine 2505 to Proline

    Available Since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNCST-avGFP

    Plasmid

    #129508

    Purpose

    Expresses avGFP (Lambert 2019 clone) constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    avGFP
    

    Expression

    Bacterial

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGP-AAV-syn-flex-iGABASnFR2-WPRE (AAV1)

    Viral Prep

    #218877-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pGP-AAV-syn-flex-iGABASnFR2-WPRE (#218877). In addition to the viral particles, you will also receive purified pGP-AAV-syn-flex-iGABASnFR2-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of the improved GABA sensor iGABASnFR2 (positive change in fluorescence). These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Promoter

    Syn

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

21. pLVX-MPro-eGFP-2

    Plasmid

    #184824

    Purpose

    Expresses Mpro-eGFP in mammalian cells

    Depositor

    Wenshe Liu

    Article

    Cao et al ACS Cent Sci. 2022 Feb 23;8(2):192-204.

    Insert

    Mpro-eGFP conjugate (M SARS-CoV-2, jellyfish Aequorea Victoria)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1alfa

    Available Since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCas3cRh

    Plasmid

    #133773

    Purpose

    Expresses all necessary components of Type I-C CRISPR-Cas system

    Depositor

    Joseph Bondy-Denomy

    Article

    Csorgo et al Nat Methods. 2020 Oct 19. pii: 10.1038/s

    Inserts

    RhaR (rhaR )
    RhaS (rhaS )
    pRhaBAD
    I-C crRNA
    Cas3
    Cas5
    Cas8
    Cas7
    

    Expression

    Bacterial

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 3xHA-miniTurbo_pAS32

    Plasmid

    #118222

    Purpose

    expresses 3xHA-tagged miniTurbo in C. elegans intestine

    Depositor

    Jessica Feldman

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Tags

    3x HA tag

    Expression

    Worm

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    ges-1p

    Available Since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHN922

    Plasmid

    #178975

    Purpose

    Expression of GST-His8-GFP-nanobody

    Depositor

    Kazuhiro Abe

    Insert

    GST-His8-GFP-nanobody
    

    Use

    Affinity Reagent/ Antibody

    Tags

    GST, His8

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV_CAG_FLEX_PKIα-P2A-mRuby3_WPRE

    Plasmid

    #196677

    Purpose

    AAV plasmid for mammalian Cre-dependent expression of PKIα-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIα (protein kinase inhibitor α) inhibits the activity of protein kinase A (PKA).

    Depositor

    Haining Zhong

    Article

    Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

    Insert

    PKIα-P2A-mRuby3
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TagBFP2-CAAX

    Plasmid

    #116856

    Purpose

    plasma membrane marker

    Depositor

    Gerry Hammond

    Article

    Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

    Insert

    HRAS
    

    Tags

    TagBFP2

    Expression

    Mammalian

    Mutation

    Amino acids 172-189

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PGK-HALO-NTERM-GFP

    Plasmid

    #185772

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    Alessandra Ianari, William Sellers

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, Halo

    Mutation

    none

    Promoter

    PGK

    Available Since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAurora2

    Plasmid

    #213134

    Purpose

    Integrated transcriptional and translational control for stringently blue-light-activated gene expression

    Depositor

    Andreas Moeglich

    Article

    Ranzani et al Nucleic Acids Res. 2024 Aug 10:gkae678.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CAV1-mCherry

    Plasmid

    #27705

    Depositor

    Ari Helenius

    Article

    Hayer et al Traffic. 2010 Mar . 11(3):361-82.

    Insert

    caveolin-1 (CAV1 Canis lupus familiaris)
    

    Tags

    monomeric Cherry

    Expression

    Mammalian

    Available Since

    July 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHR_Gal4UAS_Blinatumomab_PGK_mCherry

    Plasmid

    #85437

    Purpose

    [Edit] Lentiviral vector for Gal4 inducible Blinatumomab and constitutive mCherry expression

    Depositor

    Wendell Lim

    Article

    Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

    Insert

    Blinatumomab
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge