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Showing: 9761 - 9800 of 160697 results

  3. pET28a/Cas9-Cys

    Plasmid
    #53261
    Purpose
    Expresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal Cysteine
    Depositor
    Hyongbum Kim
    Article
    Ramakrishna et al Genome Res. 2014 Apr 2.
    Insert
    Cas9-Cys
    Use
    Tags
    6X His tag, HA epitope, and NLS
    Expression
    Bacterial
    Mutation
    Additional Cysteine is added to the C-terminal re…
    Promoter
    T7 Promoter
    Available since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    53261-plasmid-map-sequence-id-261354

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  6. p663-UBC-miniTurbo-V5-PRKACB_IDG-K

    Plasmid
    #170602
    Purpose
    Gateway destination clone of PRKACB (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-PRKACB (PRKACB Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    June 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    170602-plasmid-map-sequence-id-335769

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  11. plex_307-ccdB-hygro

    Plasmid
    #201143
    Purpose
    pLEX_307 plasmid with hygro marker
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201143-plasmid-map-sequence-id-395511

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  12. pGF-GAS4X

    Plasmid
    #225751
    Purpose
    This plasmid was designed to selectively monitor the activation of the STAT1 signaling pathway in NK cells, a pathway known to play a crucial role in regulating NK cells' activation.
    Depositor
    Eun Hee Han
    Article
    Min et al Commun Biol. 2024 Sep 30;7(1):1222. doi:
    Insert
    interferon gamma activating sequence (GAS) (STAT1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    225751-plasmid-map-sequence-id-460047

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  13. mCrimson-C1

    Plasmid
    #54806
    Purpose
    Localization: C1 Cloning Vector, Excitation: 588, Emission: 615
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    mCrimson
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. pEGFP-N1-mCherry-ApoB-TagBFP

    Plasmid
    #129383
    Purpose
    Plasmid bearing the editing target of APOBEC1 fused between the mCherry and the Tag-BFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of Tag-BFP
    Depositor
    Silvestro Conticello
    Article
    Chieca et al Methods Mol Biol. 2021;2181:69-81. doi:
    Insert
    mCherry-ApoB-Tag-BFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    May 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    129383-plasmid-map-sequence-id-254328

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  15. PB CAG-tdTomato

    Plasmid
    #133569
    Purpose
    CAG tdTomato piggyBac transposon
    Depositor
    Mario Capecchi
    Insert
    tdTomato
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133569-plasmid-map-sequence-id-274028

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  16. pSNAPf-N1

    Plasmid
    #58187
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    SNAP tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  22. TFORF2768

    Plasmid
    #142159
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    KDM5D (KDM5D Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    142159-plasmid-map-sequence-id-397675

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  25. TFORF1968

    Plasmid
    #143561
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    YY1 (YY1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143561-plasmid-map-sequence-id-400383

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  29. pSG5-hAR(16)

    Plasmid
    #89120
    Purpose
    mammalian expression of full-length human androgen receptor in pSG5, internal AR EcoRI site taken out
    Depositor
    Elizabeth Wilson
    Article
    Zhou et al Mol Endocrinol. 2002 Feb;16(2):287-300.
    Insert
    hAR (AR Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    internal EcoRI site removed
    Promoter
    SV40
    Available since
    April 11, 2017
    Availability
    Academic Institutions and Nonprofits only

  30. pGL4.22-VEGF-HRE::dLUC

    Plasmid
    #128096
    Purpose
    Hypoxia response element real-time luciferase reporter
    Depositor
    Chi Van Dang
    Article
    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:
    Insert
    Hypoxia response element (x5) from VEGF promoter (VEGFA Human)
    Use
    Luciferase
    Tags
    drives expression of destabilized luciferase (2CP…
    Expression
    Mammalian
    Mutation
    Promoter
    inserted VEGF hypoxia response elements (HRE) (x5)
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    128096-plasmid-map-sequence-id-249669

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  31. pAAV-EF1a-iCreV (AAV PHP.eB)

    Viral Prep
    #140135-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV-EF1a-iCreV (#140135). In addition to the viral particles, you will also receive purified pAAV-EF1a-iCreV plasmid DNA. EF1a-driven, light-inducible iCreV recombinase for spatiotemporally precise optogenomic modifications. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Hongkui Zeng
    Article
    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s
    Promoter
    EF1a
    Tags
    None
    Available since
    Sept. 15, 2021
    Availability
    Academic Institutions and Nonprofits only

  32. pcDNA3-TLR3-CFP

    Plasmid
    #13641
    Depositor
    Doug Golenbock
    Insert
    Toll-Like Receptor 3 (TLR3 Human)
    Use
    Tags
    CFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 12, 2007
    Availability
    Academic Institutions and Nonprofits only
    13641-plasmid-map-sequence-id-7719

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  40. pGlu57

    Plasmid
    #211560
    Purpose
    Amber Suppressor tRNA for Glutamate
    Depositor
    Liskin Swint-Kruse, Aron Fenton
    Insert
    tRNAGlu
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic (Miller 1986)
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    211560-plasmid-map-sequence-id-438928

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Showing: 9761 - 9800 of 160697 results