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163,421 results

Showing: 9761 - 9800 of 163421 results

  1. pLCKO

    Plasmid
    #73311
    Purpose
    Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BfuAI cloning sites and puromycin selection.
    Depositor
    Jason Moffat
    Article
    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Feb. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73311-plasmid-map-sequence-id-134941

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  2. CROPseq-Guide-BSD

    Plasmid
    #216123
    Purpose
    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the blasticidin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.
    Depositor
    Stefan Kubicek
    Article
    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216123-plasmid-map-sequence-id-428727

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  5. pAAV-Ef1a-Coff/Fon-GCaMP6F (AAV8)

    Viral Prep
    #137124-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Coff/Fon-GCaMP6F (#137124). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Coff/Fon-GCaMP6F plasmid DNA. EF1a-driven, Flp-dependent expression of GCaMP6f (inhibited in presence of Cre). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    EF1a
    Tags
    None
    Available since
    May 18, 2021
    Availability
    Academic Institutions and Nonprofits only

  6. pTwist GFP-Linker-VDAC1

    Plasmid
    #211735
    Purpose
    Expresses GFP tagged VDAC1 in mammalian cells. GFP is attached to the N-terminus of VDAC1 with a flexible linker.
    Depositor
    Roland Malli
    Article
    Pilic et al bioRxiv 2023.09.08.556841
    Insert
    Voltage-dependent anion channel 1 (VDAC1 )
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    211735-plasmid-map-sequence-id-424070

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  7. pLX-TRV2

    Plasmid
    #180516
    Purpose
    Agrobacterium tumefaciens-based expression of TRV2 including a PEBV heterologous promoter and apolylinker
    Depositor
    Jose-Antonio Daros
    Article
    Aragones et al Biotechnol J. 2022 Mar 24:e2100504. doi:
    Insert
    Tobacco rattle virus RNA2 with a PEBV heterologous promoter and a polylinker
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    35S CaMV
    Available since
    April 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    180516-plasmid-map-sequence-id-355071

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  12. pAAV.hSyn.FLEX.iGluSnFR3.v857.PDGFR.codonopt (AAV1)

    Viral Prep
    #175180-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.hSyn.FLEX.iGluSnFR3.v857.PDGFR.codonopt (#175180). In addition to the viral particles, you will also receive purified pAAV.hSyn.FLEX.iGluSnFR3.v857.PDGFR.codonopt plasmid DNA. Synapsin-driven, Cre-dependent, iGluSnFR3 glutamate sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Kaspar Podgorski
    Article
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Promoter
    Syn
    Tags
    None
    Available since
    July 1, 2022
    Availability
    Academic Institutions and Nonprofits only

  13. GFP::L4440

    Plasmid
    #11335
    Depositor
    Guy Caldwell
    Insert
    GFP
    Use
    RNAi
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    May 25, 2006
    Availability
    Academic Institutions and Nonprofits only
    11335-plasmid-map-sequence-id-237574

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  14. pLSB-KAN

    Plasmid
    #166700
    Purpose
    Combined sgRNA/Cas9 pLSB vector with kanMX6 (G418) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Article
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    adh15 promoter and tRNA Ser promoter
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    166700-plasmid-map-sequence-id-329224

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  16. pT7-V5-SBP-C1-HsUpf1-siRNAres_P

    Plasmid
    #147196
    Purpose
    Mammalian Expression of HsUpf1-siRNAres
    Depositor
    Elisa Izaurralde
    Insert
    HsUpf1-siRNAres (UPF1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    5 non silent mutations A69S, E167G, K282R, A442G…
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147196-plasmid-map-sequence-id-429667

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  21. VP12

    Plasmid
    #72247
    Purpose
    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A and Q926A
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72247-plasmid-map-sequence-id-131866

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  27. 5'UTR CGG 99x FMR1-FLAG

    Plasmid
    #63090
    Purpose
    Contains expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 as found in the Fragile X Tremor Ataxia Syndrome (FXTAS). These repeats are in frame with a FLAG tag.
    Depositor
    Nicolas Charlet-Berguerand
    Insert
    expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 (FMR1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63090-plasmid-map-sequence-id-104490

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  28. pLVX-EGFP-C1-centrin-1

    Plasmid
    #73331
    Purpose
    Lentiviral vector encoding centrin-1 with an N-terminal EGFP tag
    Depositor
    Manuel Thery
    Insert
    centrin-1 (CETN1 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    73331-plasmid-map-sequence-id-139044

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  29. pCMV-Luc

    Plasmid
    #45968
    Depositor
    Ralf Kuehn
    Insert
    Luciferase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 14, 2013
    Availability
    Academic Institutions and Nonprofits only
    45968-plasmid-map-sequence-id-64565

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  34. pAAV-Ef1a-fDIO-ChrimsonR-tdTomato (AAV1)

    Viral Prep
    #171027-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-Ef1a-fDIO-ChrimsonR-tdTomato (#171027). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO-ChrimsonR-tdTomato plasmid DNA. Flp-dependent, Ef1a-driven expression of ChrimsonR-tdTomato fusion for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Patricia Jensen
    Article
    Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi
    Promoter
    EF1a
    Tags
    tdTomato
    Available since
    July 21, 2023
    Availability
    Academic Institutions and Nonprofits only

  38. mCitrine-Lifeact-7

    Plasmid
    #54733
    Purpose
    Localization: Actin, Excitation: 516, Emission: 529
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    mCitrine-Lifeact
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 9761 - 9800 of 163421 results