We narrowed to 21,829 results for: SPR

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pAAV-FLEX-SaCas9-U6-sgSlc17a7

Plasmid

#124846

Purpose

Mutagenesis of Slc17a7

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc17a7 (Slc17a7 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pMH-Cas9-gate

Plasmid

#113742

Purpose

Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving hygromycin for selection in plants.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

Cas9

Use

CRISPR and Gateway Cloning

Promoter

Z. Mays ubiquitin promoter, 35S promoter

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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AAV:ITR-U6-sgRNA (Backbone) PCB-FlPO-WPRE-syntetisk pA-UTR

Plasmid

#68347

Purpose

AAV plasmid expressing the FlpO recombinase. with empty gRNA

Depositor

Jannik Elverløv-Jakobsen

Insert

FlPO

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pBZ210-pU6-sgBFP-An_CBh-sv40NLS-Cas9-Ec86RT-NLS-T2A-mCherry

Plasmid

#170185

Purpose

Expression vector for a sgRNA against BFP and spCas9-Ec86 RT fusion protein linked to mCherry via a T2A peptide. Donor template An was inserted in the Retron Ec86 msr-msd region by SpeI and AvrII.

Depositor

Hunter Fraser

Article

Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

Inserts

Ec86 RT
Retron Ec86 msr-msd
BFP-to-GFP conversion donor template An

Use

CRISPR

Tags

NLS

Expression

Mammalian

Promoter

CBh and hU6

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pT3TS-spCas9 ABE-Umax

Plasmid

#222138

Purpose

Adenine base editor with modified TadA variant for A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9 (cas9 Synthetic)

Use

CRISPR; Zebrafish expression

Tags

bipartite NLS

Promoter

T3

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pSL3402 (pTnsABf_Pse)

Plasmid

#200901

Purpose

Expresses human codon-optimized Pseudoalteromonas TnsA-TnsB fusion from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

PseTnsA-TnsB

Tags

Internal NLS between TnsA-TnsB fusion

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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L4440_BioBrick-hsf-1-sgRNA-B

Plasmid

#177787

Purpose

Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hsf-1 promoter)

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

hsf-1 (hsf-1 Nematode)

Expression

Bacterial

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pUC57-GFP-SFPQ-RT

Plasmid

#97090

Purpose

Encodes a HR repair template for knock-in of an EGFP insert that fused to the 5'-end of human SFPQ gene, without disturbing its promoter. Best used with px330-GFP-SFPQ vector.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

EGFP-SFPQ-RT (SFPQ Human)

Use

CRISPR

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-117G-hyA3A-BE4max

Plasmid

#157945

Purpose

Lentiviral expression of hyA3A-BE4max

Depositor

Dali Li

Article

Zhang et al Nat Cell Biol. 2020 Jun;22(6):740-750. d

Insert

Lenti-117G- hyA3A-BE4max

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA5_miniTurbo-C12orf49

Plasmid

#155111

Purpose

transfection plasmid for the exogneous expression of N-terminal miniTurbo-tagged C12orf49 for generation of FlpIn cell lines for BioID

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

C12orf49 (SPRING1 Human)

Tags

miniTurbo

Expression

Mammalian

Promoter

CMV/TO inducible promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pDe-CAS9-D10A

Plasmid

#61477

Purpose

based on pDe-CAS9, encodes Cas9 D10A nickase variant

Depositor

Holger Puchta

Article

Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

Insert

Cas9-D10A

Use

CRISPR

Expression

Plant

Mutation

catalytic Asp10 changed to Ala

Promoter

PcUbi4-2

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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PB-GG-OCT4-1-5-PGK-Puro

Plasmid

#102893

Purpose

PiggyBac transposon system construct with 5 concatenated U6 promoter driven transcriptional cassettes for the activation of OCT4. Contains PGK-puro selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

OCT4-gRNAs-1-5-PGK-puro (POU5F1 )

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNeo/Cre

Plasmid

#67594

Purpose

Expresses an Neomycin-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Chiou et al Genes Dev. 2015 Jul 15;29(14):1576-85. d

Insert

sgNeo

Use

CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pX458_Ruby

Plasmid

#110164

Purpose

Cas9 from S. pyogenes with Ruby2, and cloning backbone for sgRNA

Depositor

Philip Hublitz

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

hSpCas9

Use

CRISPR

Tags

3xFLAG and Ruby2

Expression

Mammalian

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pYFP-NEAT1m_v1-RT

Plasmid

#97089

Purpose

Encodes a HR repair template for knock-in a YFP expression cassette downstream of the poly(A) signal of human NEAT1_1 isoform. Best used with px330-NEAT1m_v1 vector.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

NEAT1m_v1-RT (NEAT1 Human)

Use

CRISPR

Tags

EYFP

Promoter

CMV

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti_CMV-Cas9-P2A-HygR

Plasmid

#164133

Purpose

Lentiviral construct for the expression of SpCas9 driven by CMV promoter in mammalian cells

Depositor

Hyongbum Kim

Article

Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

Insert

SpCas9 (cas9 Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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SMC2-mAC donor (Hygro)

Plasmid

#140648

Purpose

SMC2 tagging with mAID-Clover

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

SMC2 (SMC2 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Clta KI

Plasmid

#131483

Purpose

Endogenous tagging of Clathrin light chain α: N-terminal (amino acid position: M1)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Clta Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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NYESO beta into TRBC1 HDRT Source (pTR 277)

Plasmid

#112024

Purpose

DNA sequence source for amplifying an HDR template to replace endogenous human TCR-beta with 1G4 NYESO TCR-beta

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

NYESO beta into TRBC1 HDRT

Use

CRISPR and Synthetic Biology

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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M-tdTom-ST1

Plasmid

#48678

Purpose

Mammalian tdTomato activation reporter for ST1 with GTCCCCTCCACCCCACAGTG protospacer

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

TAL binding site w/ GTCCCCTCCACCCCACAGTG protospacer, ST1-PAM, and tdTomato reporter. Compatible with S. thermophilus #1 Cas9

Use

CRISPR

Promoter

Sp6

Available Since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only