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Plasmid#164850PurposegRNA vector for targeting human H11 locusDepositorInsertgRNAs for targeting human H11 locus
UseTagsExpressionMammalianMutationPromoterU6Available sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
AY27_pU6.gRNA.CLYBL
Plasmid#199238PurposeExpression construct encoding a S. pyogenes guide RNA targeting the human CLYBL safe harbor locusDepositorInsertS. pyogenes gRNA spacer
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterRNA polymerase III promoter for human U6 snRNAAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYLsgRNA-AtU3b
Plasmid#66198Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAtU3bAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6_rd12_pegRNA_(PP7-C4-Q1)
Plasmid#232435PurposepegRNA with optimized 3' modifications to correct the rd12 mutationDepositorInsert3'-PP7-tagged pegRNA for rd12 correction driven by human U6 promoter
UseCRISPRTagsExpressionMutationPromoterU6Available sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6_rd12_nsgRNA(PP7)
Plasmid#232436PurposensgRNA for PE3b correction of the rd12 mutation, contains a PP7 in the scaffold tetraloopDepositorInsertPP7-tagged nicking gRNA (PE3b) for rd12 correction driven by human U6 promoter
UseCRISPRTagsExpressionMutationPromoterU6Available sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYLsgRNA-AtU6-29
Plasmid#66203Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGGDestTol2LC-5sgRNA
Plasmid#64243Purposedestination vector for five U6x:sgRNA cassettesDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.sgRNA.EFS.tRFP657
Plasmid#57824PurposeLentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP657, EFS Promoter drivenDepositorInsertssgRNA
EFS
tagRFP657
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
VE-cad KI gRNA1
Plasmid#92310PurposeCRISPR-GFP-gRNA for cutting VEcadDepositorInsertVEcad KI gRNA1 (CDH5 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7-gRNA
Plasmid#46759PurposegRNA cloning vector for in vitro transcription of target gRNADepositorInsertgRNA core
UseCRISPR; Zebrafish expressionTagsExpressionMutationPromoterT7Available sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-CKB
Plasmid#73501PurposegRNA expression vector (with mKate2)DepositorInsertmKate2-T2A-Bsd
UseCRISPR and LentiviralTagsNLSExpressionMammalianMutationPromoterCAGAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCF142_U6-sgRNA
Plasmid#225960PurposeU6-sgRNA (recipient). Expresses U6-sgRNA (recipient) for VLP production. This is a recipient vector for cloning of specific SpyCas9 sgRNAs.DepositorInsertU6-sgRNA (recipient)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
ATG9A sgRNA
Plasmid#207557PurposepX330 expressing Cas9 and a sgRNA targeting the ATG9A locusDepositorInsertCACTGAATACCAGCGCCTAG
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
VE-cad KI gRNA2
Plasmid#92311PurposeCRISPR-GFP-gRNA for cutting VEcadDepositorInsertVEcad KI gRNA2 (CDH5 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA2-U6:gRNA
Plasmid#63157PurposeDestination vector for Gateway based on vector #394 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter.DepositorTypeEmpty backboneUseCRISPR; Tol2 destination vector for gatewayTagsExpressionMutationPromoterAvailable sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6-GG-gRNA
Plasmid#226860PurposeHuman gRNA expression vector backbone with BsmBI Golden Gate siteDepositorInsertSp-gRNA
UseTagsExpressionMammalianMutationPromoterhU6Available sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTAKN2_J23119-sgRNA
Plasmid#108552PurposeBacterial sgRNA expression vectorDepositorInsert2x Streptococcus pyogenes sgRNA
UseTagsExpressionBacterialMutationPromoterJ23119Available sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC119-gRNA
Plasmid#52255PurposeCan use a PCR template to assemble new desired guide RNA. Contains an Arabidopsis U6 promoter to drive guide RNA (targeting AtPDS3 gene target site 1) expression with a TTTTTT as terminator.DepositorInsertguide RNA targeting AtPDS3 (PDS3 Mustard Weed)
UseCRISPR; Plant expressionTagsExpressionMutationPromoterArabidopsis U6 polymerase III promoterAvailable sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
KAT5 sgRNA1
Plasmid#138187Purpose3rd generation lentiviral gRNA plasmid targeting human KAT5DepositorInsertKAT5 (KAT5 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
KAT5 sgRNA2
Plasmid#138188Purpose3rd generation lentiviral gRNA plasmid targeting human KAT5DepositorInsertKAT5 (KAT5 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only