We narrowed to 25,227 results for: nes

1. pNES-EGFP-C1-flex-PABD-Spo20

   Plasmid

   #220081

   Purpose

   Spo20-PABD PA biosensor with a flexible linker to aid in nuclear export

   Depositor

   Gerry Hammond

   Article

   Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

   Insert

   Spo20 (SPO20 Budding Yeast)
   

   Tags

   X. leavis map2k1.L(32-44)-EGFP-SGGGSGGS

   Expression

   Mammalian

   Mutation

   amino acids 51-91

   Promoter

   CMV

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40

   Plasmid

   #101060

   Purpose

   AAV vector expressing CaMPARI2 (Kd = 200nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

   Depositor

   Eric Schreiter

   Article

   Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

   Has Service

   AAV1

   Insert

   CaMPARI2
   

   Use

   AAV

   Tags

   FLAG-HA-myc and NES_his

   Promoter

   hsyn (synapsin-1)

   Available Since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGEN-dPspCas13b-4EHP-NES-mCherry

   Plasmid

   #233610

   Purpose

   Expression of catalytically-inactive PspCas13b fused with 4EHP in mammalian cells

   Depositor

   Shintaro Iwasaki

   Article

   Apostolopoulos et al Nat Commun. 2024 Mar 11;15(1):2205. doi:

   Insert

   dPspCas13b-4EHP-NES-mCherry
   

   Use

   CRISPR

   Tags

   3xFlag and mCherry

   Mutation

   H133A/H1058A

   Available Since

   March 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. LTR5HS S. pyogenes scaffold CARGO array

   Plasmid

   #191316

   Purpose

   Contains an array of guide RNAs targeting LTR5Hs elements

   Depositor

   Joanna Wysocka

   Article

   Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

   Insert

   Array of guide RNAs
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Nov. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. NLS-mCherry-NES reporter (pDN160)

   Plasmid

   #72660

   Purpose

   NLS-mCherry-NES 17 reporter for light-inducible nuclear export block with pDN157 and pDN158

   Depositor

   Barbara Di Ventura, Roland Eils

   Article

   Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

   Insert

   NLS-mCherry-NES
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. nontarget S. pyogenes scaffold CARGO array

   Plasmid

   #191319

   Purpose

   Contains an array of nontargeting guide RNAs

   Depositor

   Joanna Wysocka

   Article

   Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

   Insert

   Array of guide RNAs
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Nov. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSYN1-NES-OCaMP-WPRE

   Plasmid

   #224934

   Purpose

   pAAV (non-flex) for expressing OCaMP, an orange calcium indicator, in mammalian cells and in vivo. Contains hSyn promoter and RSET leader sequence peptide

   Depositor

   Alexander Lohman

   Article

   Aggarwal et al bioRxiv 2025.07.28.667269; doi: https://

   Insert

   OCaMP
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Promoter

   hSynI

   Available Since

   July 28, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pDV17_pCMV-MCP-ADAR2dd(E488Q)-NES

   Plasmid

   #200406

   Purpose

   Expresses a compact, engineered version of ADAR2 constitutively in mammalian cells

   Depositor

   James Collins

   Article

   Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

   Insert

   MCP-ADAR2dd(E488Q)-NES
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Mutation

   E488Q (hyperactive)

   Promoter

   CMV

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC0045-EF1a-PguCas13b-NES-HIV

   Plasmid

   #103861

   Purpose

   Expresses PguCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   PguCas13b
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and HIV NES

   Expression

   Mammalian

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-NES-DEVD-mCardinal-NLS

    Plasmid

    #164052

    Purpose

    Fluorescent reporter for caspase-3

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    NES-mCardinal-NLS
    

    Tags

    kozak sequence

    Expression

    Mammalian

    Promoter

    CMV promotor

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMD19-Cas9_handle-S.pyogenes_terminator-PBBa_J23117-Cmr

    Plasmid

    #190794

    Purpose

    Template vector to amplify pieces for creating multiplex sgRNA-arrays.

    Depositor

    Paul Hudson

    Article

    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

    Insert

    sgRNA-scaffold only
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    BBa_J23117

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC0044-EF1a-RanCas13b-NES-mapk

    Plasmid

    #103855

    Purpose

    Expresses RanCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    RanCas13b
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and mapk NES

    Expression

    Mammalian

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-myc NES-mCE NLS

    Plasmid

    #82466

    Purpose

    Expresses cytoplasmic restricted form of myc tagged mRNA capping enzyme

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    NES mRNA capping enzyme (Rngtt HIV, Mouse)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-NES-NIR-GECO1

    Plasmid

    #113683

    Purpose

    Express NIR-GECO1 preferentially in neurons

    Depositor

    Robert Campbell

    Article

    Qian et al Nat Methods. 2019 Feb;16(2):171-174. doi

    Insert

    NES-NIR-GECO1
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR_L1-Turbo-NES-YFP-STOP-L2

    Plasmid

    #127358

    Purpose

    gateway entry vector for expressing cytosolic TurboID-YFP under a promoter of choice

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NES, V5, and YFP

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV:: NES-FKBP-iLID-LRP6c

    Plasmid

    #221774

    Purpose

    Expresses NES-FKBP-iLID-LRP6c component in mammalian cells

    Depositor

    Dongmin Lee

    Article

    Lee et al iScience. 2023 Feb 19;26(3):106233. doi:

    Insert

    pCMV:: NES-FKBP-iLID-LRP6c
    

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTol2_elavl3(HuC)_NES-Caprola_01-mEGFP

    Plasmid

    #194696

    Purpose

    HuC driven expression of the calcium recorder Caprola_01 fused to mEGFP for neuronal expression in zebrafish

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_01-mEGFP
    

    Use

    Zebrafish expression

    Tags

    mEGFP

    Promoter

    elavl3(HuC)

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS2+_Flag-myc-NES-Tir1

    Plasmid

    #117717

    Purpose

    mRNA synthesis of Flag-myc-NES-TIR1 for injection

    Depositor

    Joerg Mansfeld

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Insert

    TRANSPORT INHIBITOR RESPONSE 1
    

    Tags

    Flag-Myc-NES

    Expression

    Mammalian

    Mutation

    codon optimized TIR1 for murine expression

    Promoter

    SP6

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAG1280 NLS-mCherry-FIREtag-NES

    Plasmid

    #205172

    Purpose

    expresses NLS-mCherry-FIREtag-NES in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    NLS-mCherry-FIREtag-NES
    

    Expression

    Mammalian

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV_hsyn_NES-his-CaMPARI2-F391W-L398V

    Plasmid

    #119723

    Purpose

    AAV vector expressing CaMPARI2_F391W-L398V (Kd = 174nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

    Depositor

    Thomas Oertner

    Article

    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

    Inserts

    CaMPARI2
    CaMPARI2
    

    Use

    AAV

    Tags

    NES-his

    Expression

    Mammalian

    Mutation

    F391W, L398V

    Promoter

    hSyn1

    Available Since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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