167,895 results

1. iCAP-ROOT

   Plasmid

   #192262

   Purpose

   AAV9 derived Retrograde vector Optimized for Organ Tracing (ROOT) capsid

   Depositor

   Li Ye

   Article

   Wang et al Nature. 2022 Sep;609(7927):569-574. doi:

   Insert

   Synthetic construct for ROOT VP1 gene
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   p41

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. SSTR4-Tango

   Plasmid

   #66505

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   SSTR4 (SSTR4 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQLL-Mariner2_AG

   Plasmid

   #217063

   Purpose

   Donor plasmid of Mariner2_AG Transposon

   Depositor

   Haoyi Wang

   Article

   Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

   Insert

   TIRs of Mariner2_AG transposon
   

   Expression

   Mammalian

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCh-Climp63

   Plasmid

   #136293

   Purpose

   expression of Climp63

   Depositor

   Gia Voeltz

   Article

   Shibata et al J Biol Chem. 2008 Jul 4. 283(27):18892-9

   Insert

   Cytoskeletal-linking protein 63 (Ckap4 Mouse)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. AICSDP-7:SEC61B-mEGFP

   Plasmid

   #87426

   Purpose

   Homology arms and linker-mEGFP sequence for N-terminus tagging of human SEC61B

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

   Insert

   SEC61B Homology Arms with mEGFP-linker (SEC61B Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   March 15, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pcDNA3-Mito-ExRai-AMPKAR

   Plasmid

   #192448

   Purpose

   ExRai-AMPKAR targeted to the mitochondrial outer membrane.

   Depositor

   Jin Zhang

   Article

   Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

   Insert

   Mito-ExRai-AMPKAR
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBZS-M.CviQIX (NDel15)

   Plasmid

   #198357

   Purpose

   Express M.CviQIX (NDel15) in bacterial cells.

   Depositor

   Takashi Ito

   Article

   Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

   Insert

   M.CviQIX
   

   Expression

   Bacterial

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. XPO6-EGFP

   Plasmid

   #210371

   Purpose

   Expresses XPO6 fused with EGFP in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   XPO6 (XPO6 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Syn-Chronos-GFP (AAV5)

   Viral Prep

   #59170-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV-Syn-Chronos-GFP (#59170). In addition to the viral particles, you will also receive purified pAAV-Syn-Chronos-GFP plasmid DNA. Syn-driven Chronos-GFP expression for optogenetic neural activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Promoter

   Syn

   Tags

   GFP

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

10. pPOTv7-hygro-3HaloTag

    Plasmid

    #179828

    Purpose

    Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

    Depositor

    Keith Gull, Samuel Dean

    Article

    Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

    Insert

    3x HaloTag
    

    Use

    Unspecified

    Promoter

    read through

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21-pelB-LaM-4

    Plasmid

    #172770

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-4
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJFRC-MUH

    Plasmid

    #26213

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. CROP_C9_Puro_PDGFRA

    Plasmid

    #183313

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets PDGFRA gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    PDGFRA
    

    Use

    Lentiviral

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Nsp13-mCherry

    Plasmid

    #165136

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp13 (ORF1ab codon optimized SARS-COV-2)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5/FRT/TO_Lifeact_HaloTag9

    Plasmid

    #175545

    Purpose

    Expression of HaloTag9 at the actin filaments of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Lifeact-HaloTag9
    

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV_NESPylRS(WT)_hU6tRNAPyl

    Plasmid

    #223509

    Purpose

    PylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

    Depositor

    Edward Lemke

    Article

    Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5

    Inserts

    NES Pyrrolysyl-tRNA Synthetase (pylS Methanosarcina mazei)
    tRNA Pyl
    

    Expression

    Mammalian

    Promoter

    CMV, SP6 and U6

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-CD63-L7Ae

    Plasmid

    #180503

    Purpose

    Human CD63-L7Ae fusion protein

    Depositor

    Masaharu Somiya

    Article

    Somiya et al bioRxiv 2022.01.25.477620

    Insert

    Human CD63-L7Ae fusion protein
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCXLE-Sox2AV-T2A-Klf4-E2A-Myc

    Plasmid

    #210017

    Purpose

    episomal SKM (expressing mouse Sox2-A61V + Klf4 + Myc) for integration-free naïve reset.

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    Sox2AV-t2a-Klf4-e2a-cMyc (Klf4, Sox2 Mouse)
    

    Expression

    Mammalian

    Mutation

    Sox2A61V is a point mutant of Sox2 that enhances …

    Promoter

    CAG

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQE60 R130A wgMDH

    Plasmid

    #204266

    Purpose

    Bacterial expression of R130A wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R130A

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-GFP-CD63

    Plasmid

    #194989

    Purpose

    overexpress CD63

    Depositor

    Angelique Bordey

    Insert

    GFP-CD63
    

    Expression

    Bacterial

    Available Since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pMRE-Tn7-165

    Plasmid

    #118577

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mScarlet
    

    Use

    Minitn7 delivery vector

    Expression

    Bacterial

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAAV-synapsin-HaloCaMP1a-EGFP

    Plasmid

    #138327

    Purpose

    chemigenetic calcium indicator

    Depositor

    Eric Schreiter

    Article

    Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

    Has Service

    AAV1 and AAV9

    Insert

    HaloCaMP1a
    

    Use

    AAV

    Tags

    EGFP, His-tag, and Nuclear Exclusion Signal

    Expression

    Mammalian

    Promoter

    synapsin

    Available Since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. pTRANS_250d

    Plasmid

    #91124

    Purpose

    Transformation, Type: T-DNA, Plant Selection: PvUbi2:hpt II, Viral Replication: none

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    PvUbi2

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. pHS2 (hANGPTL4-V5)

    Plasmid

    #102446

    Purpose

    Expresses V5-tagged human ANGPTL4 in mammalian cells

    Depositor

    Brandon Davies

    Article

    Chi et al J Biol Chem. 2015 May 8;290(19):11865-77

    Insert

    ANGPTL4 (ANGPTL4 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. paavCAG-pre-mGRASP-mCerulean

    Plasmid

    #34910

    Purpose

    pre

    Depositor

    Jinhyun Kim

    Article

    Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

    Has Service

    AAV5

    Insert

    presynaptic mGRASP
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    Nov. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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26. MAPK8

    Plasmid

    #73236

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MAPK8 (MAPK8 Human)
    

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    pfam00069:Pkinase 26-321

    Promoter

    T7

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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27. pGEX-6P-1_WT RAD23B

    Plasmid

    #201457

    Purpose

    Expresses human RAD23B with an N-terminal GST tag in E. coli

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog B (RAD23B Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Coding sequence has been optimised for expression…

    Promoter

    tac promoter

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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28. pOT_6 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-tNGFR

    Plasmid

    #181975

    Purpose

    Expresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

    Insert

    FMC6.3-BBz CAR; tNGFR (NGFR Human, Synthetic)
    

    Use

    Lentiviral

    Mutation

    NGFR truncation: aa 11-276

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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29. pExchange_tdk_BT1311p-tetR-1

    Plasmid

    #90325

    Purpose

    Suicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 4861701 and 4861702

    Depositor

    Andrew Goodman

    Article

    Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

    Insert

    TetR
    

    Expression

    Bacterial

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJJS001

    Plasmid

    #188324

    Purpose

    PCR template for repair templates to tag a gene with a GFP and mIAA7 AID degron using homology-directed repair for C. elegans.

    Depositor

    Mike Boxem

    Article

    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

    Insert

    mIAA7::GFP::Linker
    

    Expression

    Bacterial

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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31. pBS-L1PA1CH blast

    Plasmid

    #69612

    Purpose

    Expresses a blasticidin tagged codon optimized L1PA1

    Depositor

    Astrid Roy-Engel

    Insert

    L1PA1
    

    Tags

    blast cassette

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.0 FLAG TRPV4

    Plasmid

    #45751

    Depositor

    Robert Lefkowitz

    Article

    Shukla et al J Biol Chem. 2010 Sep 24;285(39):30115-2

    Insert

    TRPV4 (Trpv4 Rat)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMV306DIhsp+LuxG13

    Plasmid

    #49999

    Purpose

    Integrase removed to increase stability of the plasmid in mycobacteria

    Depositor

    Brian Robertson

    Article

    Andreu et al J Antimicrob Chemother. 2013 Sep;68(9):2

    Insert

    Bacterial luciferase operon + G13 promoter
    

    Expression

    Bacterial

    Mutation

    It contains a gram-positive enhanced translation …

    Promoter

    G13 from M. marinum G13 promoter driving luxC

    Available Since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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34. pcDNA-BRCA1-mCherry

    Plasmid

    #195275

    Purpose

    express mCherry-BRCA1 fusion

    Depositor

    Xiaokun Shu

    Article

    Li et al Sci Adv. 2023 Mar;9(9):eade3760. doi: 10

    Insert

    mCherry-BRCA1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. bdSUMO-HA2-GFP-HiBit

    Plasmid

    #171017

    Purpose

    For bacterial expression of bdSUMO tagged muGFP with HA2 peptide and NanoLuc complementation peptide variant #86

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    14x-His-bdSUMO-HA2-muGFP-HiBiT
    

    Use

    Luciferase

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. TFORF0794

    Plasmid

    #142239

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ATF7 (ATF7 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDONR221_SLC7A9

    Plasmid

    #132245

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC7A9 (SLC7A9 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 21, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pTXB1-AbaSFI

    Plasmid

    #86900

    Purpose

    Express CBD and intein tag containing AbaSI enzyme full length

    Depositor

    Matthias Bochtler

    Article

    Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

    Insert

    AbaSDFI
    

    Tags

    CBD

    Promoter

    T7

    Available Since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pInducer-YAP1

    Plasmid

    #213583

    Purpose

    Doxycycline inducible expression of YAP1 wild type cDNA in mammalian cells

    Depositor

    Channing Der

    Article

    Edwards et al Cancer Res. 2023 Nov 7. doi: 10.1158/000

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pHJ12: CadC-VHH-Caffeine (No linker)

    Plasmid

    #108244

    Purpose

    Membrane bound transcription activator CadC fusing VHH-Caffeine. Upon ligand binding, CadC-VHH-Caffeine can be dimerized and activate down stream reporter gene sfGFP expression

    Depositor

    Jerome Bonnet

    Article

    Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

    Insert

    Membrane bound transcription activator CadC fusing with VHH-Caffeine antibody without external linker
    

    Use

    Synthetic Biology

    Promoter

    pLacO1

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge