161,492 results

1. pCAX Itgb1-FLAG

   Plasmid

   #30153

   Depositor

   Tracy Young-Pearse, Dennis Selkoe

   Article

   Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

   Insert

   integrin beta 1 (Itgb1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pCMV-Frankenbody-FLAG-SNAP

   Plasmid

   #175295

   Purpose

   Track mature FLAG tagged proteins in live cells

   Depositor

   Tim Stasevich

   Article

   Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

   Insert

   anti-FLAG frankenbody fused with SNAP-tag
   

   Use

   Tags

   SNAP-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 19, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pCDNA3-mouse PKA-RIIalpha-mEGFP

   Plasmid

   #45527

   Depositor

   Haining Zhong

   Article

   Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

   Insert

   PKA regulatory subunit RII alpha tagged by mEGFP
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBTK622

   Plasmid

   #180592

   Purpose

   tdk/kanR dual selection cassette for engineering the genome of Acinetobacter baylyi ADP1

   Depositor

   Jeffrey Barrick

   Article

   Suarez et al Nucleic Acids Res. 2020 May 7;48(8):4585

   Inserts

   tdk
   kanR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3 IKKE K38A flag

   Plasmid

   #26202

   Depositor

   Tom Maniatis

   Article

   Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

   Insert

   I-kappa-B kinase epsilon K38A (IKBKE Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Catalytic Lysine changed to Alanine (K38A)

   Promoter

   Available since

   Aug. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pInducer-YAP1-S127A/S397A/S94A

   Plasmid

   #213586

   Purpose

   Doxycycline inducible expression of YAP1-S127A/S397A/S94A cDNA in mammalian cells

   Depositor

   Channing Der

   Article

   Edwards et al Cancer Res. 2023 Nov 7. doi: 10.1158/000

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S127A, S397A, S94A

   Promoter

   Available since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-UBE4B

   Plasmid

   #113499

   Purpose

   Expression of human UBE4B with N-terminal GFP tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   UBE4B (UBE4B Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR- flag-Lag16-NCAM-1

   Plasmid

   #205199

   Purpose

   This vector encodes of the synCAM (NCAM1) and GFP nanobody LAG16

   Depositor

   Wendell Lim

   Article

   Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

   Insert

   synCAM NCAM 1 and Lag16 anti-GFP nanobody
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-tetO-Tfap2c

   Plasmid

   #70269

   Purpose

   Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Tfap2c

   Depositor

   Hubert Schorle

   Article

   Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

   Insert

   transcription factor AP-2, gamma (Tfap2c Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetO

   Available since

   Nov. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBAD.Sigma32.I54N

    Plasmid

    #59982

    Purpose

    Expresses sigma32 I54N mutant in E. coli to render a heat-shock like cellular folding environment

    Depositor

    Jeffery Kelly

    Article

    Zhang et al ACS Chem Biol. 2014 Jul 22.

    Insert

    Sigma32 I54N
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    I54N

    Promoter

    Available since

    Nov. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. mCerulean3-mVenus-CASPER

    Plasmid

    #58162

    Purpose

    Localization: Cytosolic FRET, Excitation: , Emission:

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    mCerulean3 and mVenus-CASPER

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBbE7a-RFP

    Plasmid

    #35313

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TFORF0981

    Plasmid

    #141778

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    KLF6 (KLF6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. plvx-tetO-GFP

    Plasmid

    #191560

    Purpose

    Inducible expression of GFP

    Depositor

    Joseph Sodroski, Alon Herschhorn

    Article

    Herschhorn et al PLoS One. 2011;6(11):e26731. doi: 10.137

    Insert

    gfp
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCN-mBFP

    Plasmid

    #63847

    Purpose

    Encodes a NADPH dependent blue fluorescent protein expressed under a strong promoter and strong RBS without His-tagged.

    Depositor

    Howard Salis

    Article

    Ng et al Metab Eng. 2015 May;29:86-96. doi: 10.10

    Insert

    mBFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter and RBS was swapped. His-tag was removed…

    Promoter

    Available since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Hbb

    Plasmid

    #99692

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Hbb, vector allows for strong activation of mouse Hbb, can be packaged and delivered as AAV

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9 and gRNA targeting Hbb (Hbb Synthetic)
    

    Use

    AAV

    Tags

    VPR mini

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-BLRR

    Plasmid

    #158958

    Purpose

    Bioluminescent repair reporter (BLRR) for DNA double strand break (DSB) repairs: homology-directed repair (HDR) and non-homologous end joining (NHEJ).

    Depositor

    Charles P. Lai

    Article

    Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

    Insert

    BLRR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMW41: pET-His-MBP-SUMO-R2Bm-SII

    Plasmid

    #209290

    Purpose

    Expresses the R2Bm ORF in E. coli

    Depositor

    Feng Zhang

    Article

    Wilkinson et al Science. 2023 Apr 21;380(6642):301-308.

    Insert

    R2
    

    Use

    Tags

    His, MBP, StrepII, and bdSUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-trGluc

    Plasmid

    #158959

    Purpose

    DNA donor plasmid containing repair template for Gaussia luciferase (Gluc) of bioluminescent repair reporter (BLRR).

    Depositor

    Charles P. Lai

    Article

    Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

    Insert

    trGluc
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. SGEP-sh-Hs-PHGDH-1956

    Plasmid

    #188670

    Purpose

    shRNA

    Depositor

    Lewis Cantley

    Insert

    shPHGDH (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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