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Plasmid#200213PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas1 H25A)DepositorInsertPaCas1, PaCas2-3
UseTagsStrep II-TEV on Cas1ExpressionBacterialMutationCas1 H25A mutationPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-458
Plasmid#200214PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas1 E184A)DepositorInsertPaCas1, PaCas2-3
UseTagsStrep II-TEV on Cas1ExpressionBacterialMutationCas1 E184A mutationPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-459
Plasmid#200215PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas1 K214A)DepositorInsertPaCas1, PaCas2-3
UseTagsStrep II-TEV on Cas1ExpressionBacterialMutationCas1 K214A mutationPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-460
Plasmid#200216PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas2 R55E, N56D)DepositorInsertPaCas1, PaCas2-3
UseTagsStrep II-TEV on Cas1ExpressionBacterialMutationCas2 R55E-N56D mutationsPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-461
Plasmid#200217PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E)DepositorInsertPaCas1, PaCas2-3
UseTagsStrep II-TEV on Cas1ExpressionBacterialMutationCas2 K11D-R12E mutationsPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-462
Plasmid#200218PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E, R55E, N56D)DepositorInsertPaCas1, PaCas2-3
UseTagsStrep II-TEV on Cas1ExpressionBacterialMutationCas2 K11D-R12E, R55E-N56D mutationsPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLeo-B-a2-GEMS(JAK-STAT)
Plasmid#209112PurposeTransient mammalian expression of the CC functionalized GEMS receptor B-a2-GEMS (JAK-STAT)DepositorInsertB-a2-GEMS(JAK-STAT)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLeo-G-a2-GEMS(JAK-STAT)
Plasmid#209113PurposeTransient mammalian expression of the CC functionalized GEMS receptor G-a2-GEMS (JAK-STAT)DepositorInsertG-a2-GEMS(JAK-STAT)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMtnA FLAG-IntS7 puro
Plasmid#208405PurposeExpresses FLAG-tagged Drosophila IntS7 from inducible MtnA promoterDepositorInsertpMT FLAG MCS puro (defl Fly)
UseTags3xFLAGExpressionInsectMutationPromoterMtnAAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMtnA FLAG-IntS13 puro
Plasmid#208406PurposeExpresses FLAG-tagged Drosophila IntS13 from inducible MtnA promoterDepositorInsertIntS13 (asun Fly)
UseTags3xFLAGExpressionInsectMutationPromoterMtnAAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMtnA FLAG-IntS14 puro
Plasmid#208407PurposeExpresses FLAG-tagged Drosophila IntS14 from inducible MtnA promoterDepositorInsertIntS14 (IntS14 Fly)
UseTags3xFLAGExpressionInsectMutationPromoterMtnAAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-Zebrafish IntS6
Plasmid#198410PurposeExpresses FLAG-tagged zebrafish IntS6DepositorInsertints6 (ints6 Zebrafish)
UseTags3xFLAGExpressionInsectMutationPromoterUbi-p63eAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-Zebrafish IntS6L
Plasmid#198411PurposeExpresses FLAG-tagged zebrafish IntS6LDepositorInsertints6l (ints6l Zebrafish)
UseTags3xFLAGExpressionInsectMutationPromoterUbi-p63eAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMtnA FLAG-IntS6 puro
Plasmid#195076PurposeExpresses FLAG-tagged Drosophila IntS6 from inducible MtnA promoterDepositorInsertIntS6 (IntS6 Fly)
UseTags3xFLAGExpressionInsectMutationPromoterMtnAAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMtnA FLAG-IntS12 puro
Plasmid#195077PurposeExpresses FLAG-tagged Drosophila IntS12 from inducible MtnA promoterDepositorInsertIntegrator 12 (IntS12 Fly)
UseTags3xFLAGExpressionInsectMutationPromoterMtnAAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-IntS6 AA 1-400
Plasmid#195071PurposeExpresses FLAG-tagged Drosophila IntS6 amino acids 1-400DepositorInsertIntS6 (IntS6 Fly)
UseTags3xFLAGExpressionInsectMutationPromoterUbi-p63eAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-IntS6 AA 1-600
Plasmid#195072PurposeExpresses FLAG-tagged Drosophila IntS6 amino acids 1-600DepositorInsertIntS6 (IntS6 Fly)
UseTags3xFLAGExpressionInsectMutationPromoterUbi-p63eAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-IntS6 AA 101-1284
Plasmid#195073PurposeExpresses FLAG-tagged Drosophila IntS6 amino acids 101-1284DepositorInsertIntS6 (IntS6 Fly)
UseTags3xFLAGExpressionInsectMutationPromoterUbi-p63eAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-IntS6 AA 1197-1284
Plasmid#195074PurposeExpresses FLAG-tagged Drosophila IntS6 amino acids 1197-1284DepositorInsertIntS6 (IntS6 Fly)
UseTags3xFLAGExpressionInsectMutationPromoterUbi-p63eAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-IntS6 AA 101-1200
Plasmid#195075PurposeExpresses FLAG-tagged Drosophila IntS6 amino acids 101-1200DepositorInsertIntS6 (IntS6 Fly)
UseTags3xFLAGExpressionInsectMutationPromoterUbi-p63eAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCOA-Leu-FvPPT1-fsr1
Plasmid#179868PurposeConstitutive expression vector for Saccharomyces cerevisiae comprising the polyketide synthase fsr1 from Fusarium vanettenii and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1DepositorInsertsPPT1
fsr1
UseTagsExpressionYeastMutationCodon optimized for expression in Saccharomyces c…PromoterYarrowia lipolytica EXP1 and Yarrowia lipolytica …Available sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-HAPPID1
Plasmid#204626PurposeExpresses the human anti-Phl p 7 IgD/λ antibody 102.1F10 (HAPPID1) in mammalian cells. HAPPID1 binds to the grass pollen allergen Phl p 7 with subnanomolar affinity.DepositorInsertsHAPPID1 heavy chain
HAPPID1 light chain
UseTagsExpressionMammalianMutationPromotermouse elongation factor 1 alpha and rat elongatio…Available sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-HA_aa 321-843 REV1
Plasmid#201587PurposeExpresses the catalytic domain (aa 321-843) of human DNA repair protein REV1 with a HA tag in mammalian cellsDepositorInsertDNA repair protein REV1 (REV1 Human)
UseTagsHAExpressionMammalianMutationExpresses amino acids 321-843 of DNA repair prote…PromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pet28b_His_RAD23A-UBA1
Plasmid#201549PurposeExpresses UBA1 of human RAD23A in E. coliDepositorInsertUV excision repair protein RAD23 homolog A (RAD23A Human)
UseTags6x HisExpressionBacterialMutationExpresses amino acids 160-205 of DNA polymerase i…PromoterT7Available sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_S172A_M173A RAD23A
Plasmid#201574PurposeExpresses a variant of human RAD23A containing mutations S172A and M173A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23ADepositorInsertUV excision repair protein RAD23 homolog A (RAD23A Human)
UseTagsFLAGExpressionMammalianMutationContains mutations S172A and M173APromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_V195A RAD23A
Plasmid#201575PurposeExpresses a variant of human RAD23A containing mutation V195A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23ADepositorInsertUV excision repair protein RAD23 homolog A (RAD23A Human)
UseTagsFLAGExpressionMammalianMutationContains mutation V195APromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_S172A_M173A_V195A RAD23A
Plasmid#201576PurposeExpresses a variant of human RAD23A containing mutations S172A, M173A and V195A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23ADepositorInsertUV excision repair protein RAD23 homolog A (RAD23A Human)
UseTagsFLAGExpressionMammalianMutationContains mutations S172A, M173A and V195APromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_C344A RAD23A
Plasmid#201577PurposeExpresses a variant of human RAD23A containing mutation C344A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23ADepositorInsertUV excision repair protein RAD23 homolog A (RAD23A Human)
UseTagsFLAGExpressionMammalianMutationContains mutation C344APromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_F354A RAD23A
Plasmid#201578PurposeExpresses a variant of human RAD23A containing mutation F354A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23ADepositorInsertUV excision repair protein RAD23 homolog A (RAD23A Human)
UseTagsFLAGExpressionMammalianMutationContains mutation F354APromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_C344A_F354A RAD23A
Plasmid#201579PurposeExpresses a variant of human RAD23A containing mutations C344A and F354A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23ADepositorInsertUV excision repair protein RAD23 homolog A (RAD23A Human)
UseTagsFLAGExpressionMammalianMutationContains mutations C344A and F354APromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only