We narrowed to 15,723 results for: mpl
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Plasmid#111155Purposeknock in bait for Axolotl Tyrosinase locusDepositorInsertTyrosinase-bait-T2a-Cherry-pA
ExpressionMammalianMutationwtAvailable SinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shNup88-HindIII
Plasmid#87329PurposeTo express shRNA against human Nup88DepositorInsertshRNA against human Nup88
UseRNAiExpressionMammalianPromoterH1Available SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shNup93-XhoI
Plasmid#87330PurposeTo express shRNA against human Nup93DepositorInsertshRNA against human Nup93
UseRNAiExpressionMammalianPromoterH1Available SinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDGE77
Plasmid#80579Purposerecipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, BASTADepositorInsertspnos:PAT-tnos
p2x35S:Cas9 D10A-tnos
BsaI-ccdB_CmR-BsaI
UseCRISPRExpressionPlantMutationD10AAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE78
Plasmid#80580Purposerecipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, nptIIDepositorInsertspnos:nptII-tmos
pPcUbi:Cas9 D10A-tnos
BsaI-ccdB_CmR-BsaI
UseCRISPRExpressionPlantMutationD10AAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE79
Plasmid#80581Purposerecipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, BASTADepositorInsertspnos:PAT-tnos
pPcUbi:Cas9 D10A-tnos
BsaI-ccdB_CmR-BsaI
UseCRISPRExpressionPlantMutationD10AAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Ywhag-3'UTR-MRE-3 MUT
Plasmid#61792PurposeLuciferase reporter containing the 3'UTR of mouse Ywhag with MRE-3 mutatedDepositorInsertmouse Ywhag 3'UTR with miR-200c MRE-3 mutated
UseLuciferaseMutationmiR-200c MRE-3 mutatedAvailable SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA2 L171C
Plasmid#66177PurposeExpresses the L171C mutant of Sulfolobus solfataricus PCNA2DepositorInsertDNA polymerase sliding clamp 2
TagsHis6 tagExpressionBacterialMutationL171CPromoterT7 promoterAvailable SinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA1 P186C
Plasmid#66173PurposeExpresses the P186C mutant of Sulfolobus solfataricus PCNA1DepositorInsertDNA polymerase sliding clamp 1
TagsHis6 tagExpressionBacterialMutationP186CPromoterT7 promoterAvailable SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA1 G108C
Plasmid#66140PurposeExpresses the G108C mutant of Sulfolobus solfataricus PCNA1DepositorInsertDNA polymerase sliding clamp 1
TagsHis6 tagExpressionBacterialMutationG108CPromoterT7 promoterAvailable SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
HLH Bmal Pas 1-465-VenC I317D
Plasmid#47367PurposeBmal fragment cloned with Venus tag used for mutationDepositorInsertBMAL (Bmal1 Mouse)
TagsVenus aa 156-239ExpressionMammalianMutationaa 1-465, I317DPromoterCMVAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pClneoFLAG human Dendrin
Plasmid#47934Purposeexpresses FLAG tagged human Dendrin in mammalian cellsDepositorInsertDendrin-1 (DDN Human)
TagsFLAGExpressionMammalianMutationcontains amino acids 2-711 of human DendrinPromoterCMVAvailable SinceSept. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSG5-FLAG-CaMKKalpha RP deletion
Plasmid#33328DepositorInsertCalcium/Calmodulin dependent protein kinase kinase alpha (Camkk1 Rat)
TagsFLAGExpressionMammalianMutationDeleted RP domain (amino acids 167-189 missing)PromoterSV40Available SinceApril 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T3-ZO-2-1st-PDZ
Plasmid#27424DepositorAvailable SinceMarch 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-SapI
Plasmid#196082Purpose(Empty Backbone) Inducible CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.DepositorInsertsdCas9-KRAB-MeCP2 repressor
hU6 promoter and gRNA scaffold
Aminoglycoside phosphotransferase
UseCRISPRExpressionMammalianMutationCatalytically dead mutant of the Cas9 endonucleas…Available SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRPL28(K55M) SpHIS5 (pNTI666)
Plasmid#115436PurposeExpresses K55M RPL28 from HIS3 yeast plasmidDepositorInsertsExpressionYeastMutationK55M; MfeI site in intronPromoterAshbya gospii TEF1 and endogenousAvailable SinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 p37 deltaUBX
Plasmid#113504PurposeBacterial expression of GST-tagged p37 lacking the UBX domainDepositorInsertp37 (Ubxn2b Mouse)
TagsGST-PreScission protease siteExpressionBacterialMutationdelta 251-331 (stop codon behind AA250)Available SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-mRIN2 dRA
Plasmid#108941PurposeMammalian expression of mRIN2 dRADepositorInsertRas and Rab interactor 2 (Rin2 Mouse)
TagsHAExpressionMammalianMutationDeletion of RA domain (aa 751-836)PromoterCMVAvailable SinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-mRIN2 dRHVPS9
Plasmid#108942PurposeMammalian expression of mRIN2 dRHVPS9DepositorInsertRas and Rab interactor 2 (Rin2 Mouse)
TagsHAExpressionMammalianMutationDeletion of RHVPS9 domain (aa 455-738)PromoterCMVAvailable SinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
GL2 shRNA
Plasmid#86083PurposeA lentiviral construct for building stable cell lines expressing GL2 shRNA via puromycin selection.DepositorInsertcontrol shRNA
PromoterU6Available SinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDF-casBCDE(+6)
Plasmid#89728PurposeExpresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a longer version of the wt spacer (+6) extended by 6 nucleotides at the leader-distal end.DepositorInsertCRISPR array
ExpressionBacterialMutationDerivative CRISPR array containing a longer versi…Available SinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSR04
Plasmid#69151Purposeread-outloxP mCherry to GFP switch, with eft-1::tagBFP::tbb-2UTR as gene of interest for integration on cxtTi10816, Mos Chr IVDepositorInsertsmCherry
TagBFP
eGFP
UseCre/Lox; MossciExpressionWormMutationcodon-optimzed index 1.0Promotereef-1A.1 (eft-3) and rps-27Available SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cerulean(159-238) in pcDNAI/Amp
Plasmid#60898PurposeThis is a vector that attaches Cerulean(159-238) to the N-terminus of a protein.DepositorInsertCerulean(159-238)
ExpressionMammalianMutationContains N164H substitutionPromoterCMVAvailable SinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA3 R112C/T180C
Plasmid#66182PurposeExpresses the R112C/T180C mutant of Sulfolobus solfataricus PCNA3DepositorInsertDNA polymerase sliding clamp 3
TagsHis6 tagExpressionBacterialMutationR112C, T180CPromoterT7 promoterAvailable SinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA2 E105C/L171C
Plasmid#66178PurposeExpresses the E105C/L171C mutant of Sulfolobus solfataricus PCNA2DepositorInsertDNA polymerase sliding clamp 2
TagsHis6 tagExpressionBacterialMutationE105C, L171CPromoterT7 promoterAvailable SinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO myc Separase delta 55/S1126A
Plasmid#59825PurposeAllows the integration of myc Separase delta 55/S1126A in the genome and Tet-inducible expression.DepositorInsertSeparase (ESPL1 Human)
TagsMycExpressionMammalianMutationdelta 55/S1126APromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable SinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
M26I DJ-1 TEV site pET15b
Plasmid#60685PurposeRecombinant protein expression of Park7DepositorInsertM26I DJ-1 (PARK7 Human)
Tags6xHisExpressionBacterialMutationM26I and TEV site was inserted (see comments belo…PromoterT7Available SinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
M26L DJ-1 TEV site pET15b
Plasmid#60686PurposeRecombinant protein expression of Park7DepositorInsertM26L DJ-1 (PARK7 Human)
Tags6xHisExpressionBacterialMutationM26L and TEV site was inserted (see comments belo…PromoterT7Available SinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc-AP1S3 R33W
Plasmid#58294Purposemammalian expression of AP1S3 R33W mutantDepositorInsertAP1S3 (AP1S3 Human)
Tags6xHis and mycExpressionMammalianMutationR33W: c.97C>T,p.Arg33TrpPromoterCMVAvailable SinceAug. 1, 2014AvailabilityAcademic Institutions and Nonprofits only