We narrowed to 18,995 results for: Met;
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Plasmid#61708PurposeYeast integration plasmid targeted to the HO loci with heterodimeric Hsp82. Digest with NotI prior to addition to yeast.DepositorInsertHsp82viiA (HSP82 Budding Yeast)
UseYeast integration vector targeting inserts to the…Tags6xHisExpressionYeastMutationHeteromeric coiled coil inserted after amino acid…PromoterTEF1Available SinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
FLAG-GK in PLJM60
Plasmid#154207PurposeLentiviral vector for stable expression of GKDepositorAvailable SinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-APEX2-F
Plasmid#160083PurposeBacterial expression of C-terminally FLAG-tagged APEX2 peroxidaseDepositorInsertAPEX2 peroxidase
TagsFLAG and Mxe intein - Chitin-binding domainExpressionBacterialAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPAP_BGL1
Plasmid#183735PurposeExpresses Aspergillus aculeatus β-glucosidase (BGL1)DepositorInsertβ-glucosidase (AaBGL1)
ExpressionYeastPromoterAOX1 promoterAvailable SinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCX-Lyn-SpiCee
Plasmid#140837Purposemammalian expression of Lyn-SpiCee, a lipid raft-targeted variant of the calcium buffer SpiCee that prevents downstream signalingDepositorInsertLyn-SpiCee
TagsmRFPExpressionMammalianPromoterCAGAvailable SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
mEos2-Integrin-Beta1-N-18
Plasmid#57390PurposeLocalization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581DepositorAvailable SinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTK47_mCherry-LGN-hardened
Plasmid#46347Purposerescues the phenotypes caused by LGN RNAiDepositorInsertLGN (GPSM2 Human)
TagsmCherryExpressionMammalianMutationsiRNA resistant, gaGTtGacTgcTcgTTtA; deletion of …PromoterCMVAvailable SinceAug. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCIFR-3
Plasmid#233295PurposeIntegration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette used for selecting for the integration can be removed in a later stage with pFNC.DepositorInsertmsfGFP
Promoterp14GAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLEx-TetOn3G-3xHA-FLEx
Plasmid#133729PurposePlasmid contains the floxed TetOn3G-transactivator sequence for expression of TetOn3G in the presence of Cre recombinase.DepositorInsertTetOn3G-3xHA (floxed & inverted)
UseAAVTags3xHA-tagExpressionMammalianPromoterpCMVAvailable SinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/Myc-DNMT3B2
Plasmid#36942DepositorAvailable SinceJune 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDS2142
Plasmid#184273PurposeTet-off genome insertion systemDepositorInsertCatTA
ExpressionYeastPromoterTDH3Available SinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2340_Tier3(SB)-iRFP_Puro
Plasmid#169638PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven iRFP670 and PuroR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCX-SpiCee-Kras
Plasmid#140838Purposemammalian expression of SpiCee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the calcium buffer SpiCee that prevents downstream signalingDepositorInsertSpiCee-Kras
TagsmRFPExpressionMammalianPromoterCAGAvailable SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVH64-Tier1-PSV40-Citrine_p2a_SEAP
Plasmid#169558PurposeTier-1 vector encoding PSV40-driven Citrine-p2A-SEAP expression (PSV40-Citrine-p2A-SEAP-pA).DepositorInsertPSV40-driven citrine-P2A-SEAP fusion construct
ExpressionMammalianPromoterPSV40Available SinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
FoxO1 KR
Plasmid#66166PurposeExpresses FoxO1 with three Lys residues surrounding Ser-253 that are known to be sites of acetylation were replaced by arginine residues.DepositorAvailable SinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-Nup35-mut
Plasmid#87341PurposeTo express human Nup35 in mammalian cells. It contains synonymous mutations that are not targeted by shRNADepositorInsertNUP35 (NUP35 Human)
TagsEGFPExpressionMammalianMutationshRNA resistant changesPromoterCMVAvailable SinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-ChRger2-TS-EYFP
Plasmid#127238PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.DepositorInsertChRger2-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianPromoterCamKIIaAvailable SinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pROF417
Plasmid#155335PurposeConstitutive expression LFY-VP16-2A-RLuc in plantsDepositorInsertPCaMV35S-LFY-VP16-NLS-2A-RLuc-Tnos
UseLuciferase and Synthetic BiologyExpressionPlantAvailable SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSNAP-CLC-EYFP
Plasmid#38011DepositorInsertClathrin, light polypeptide (LCa) (Clta Mouse)
TagsEYFP and SNAP26mExpressionBacterial and MammalianPromoterCMVAvailable SinceSept. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pet28a-His6-Halo-Tev-Keap1
Plasmid#62456PurposeHis6-halo tagged human Keap1 protein for E.coli expressionDepositorAvailable SinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-tagRFP-NLS-HA-Bxb1
Plasmid#65625PurposeVector for cotranslational expression of tagRFP-Bxb1DepositorInsertBxb1 integrase
TagsHA, SV40 NLS, and tagRFPExpressionMammalianPromoterCAGAvailable SinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
FoxO1 KA
Plasmid#66164PurposeExpresses FoxO1 with three Lys residues surrounding Ser-253 that are known to be sites of acetylation were replaced by alanine residues.DepositorAvailable SinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-H2B-3xFLAG-2A-G(N2c)
Plasmid#194355PurposeAAV constitutively expressing nuclear FLAG and Rabies Glycoprotein (G) of CVS-N2c strainDepositorInsertsH2B-3xFLAG
Rabies CVS-N2c glycoprotein (G)
UseAAVTagsFLAGAvailable SinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBMN-AS-HNF4α
Plasmid#139305PurposeKnocking down of human HNF4α through shRNA and amiRNA targeting HNF4αDepositorInsertshRNA and amiRNA against HNF4α
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDendra2-H4
Plasmid#157815PurposeFluorescent reporter for histone H4 trackingDepositorAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-mCherry-ApoB-TagBFP
Plasmid#129383PurposePlasmid bearing the editing target of APOBEC1 fused between the mCherry and the Tag-BFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of Tag-BFPDepositorInsertmCherry-ApoB-Tag-BFP
ExpressionMammalianPromoterCMV promoterAvailable SinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
FoxO1 KQ
Plasmid#66165PurposeExpresses FoxO1 with three Lys residues surrounding Ser-253 that are known to be sites of acetylation were replaced by glutamine residues.DepositorAvailable SinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MBP-CBX1-eReader
Plasmid#135089PurposeBacterial expression of engineered CBX1 with enhanced binding to H3K9meDepositorInsertCBX1 chromobox protein 1, engineered
Tags6xhis-tag and MBPExpressionBacterialMutationlysine 43 mutated to alanine; aspartic acid 59 mu…PromoterT7Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
GluR2_adRNA(1,20,6)-ADAR2(E488Q)
Plasmid#124621PurposeExpresses adRNA(1,20,6) targeting the RAB7A transcript along with the ADAR2(E488Q)DepositorInsertADAR2(E488Q)
UseAAVExpressionMammalianMutationE488Q hyperactive mutantPromoterCMVAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-SAMTOR (D190A)
Plasmid#100515PurposeExpresses HA tagged SAMTOR D190A mutant in mammalian cellsDepositorAvailable SinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast coUGP2
Plasmid#211531PurposeExpresses codon optimized UGP2DepositorAvailable SinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMO746
Plasmid#117480Purposeupp in an artificial operon with npt from pMO9071 and AmpR-pUC ori from pCR4/TOPO, Pnpt-npt-upp;, KanRDepositorInserturacil phosphoribosyltransferase
ExpressionBacterialAvailable SinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
SEH1L in pRK5
Plasmid#154216PurposeUntagged SEH1L cDNA expression vector for transfectionsDepositorAvailable SinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCHC005 (∆RE)
Plasmid#226199PurposeConjugation knockout plasmid (pK18mobsacB) to delete H16_0006 (Type I restriction endonuclease subunit).DepositorInsertHomology arms for type I restriction-modification system restriction subunit H16_A0006
UseSynthetic BiologyAvailable SinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGREAT2
Plasmid#170890PurposeGoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_NOSt+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…Promoterp35SoTMVAvailable SinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry_EHMT1_full length
Plasmid#228810PurposeExpression of mouse EHMT1DepositorAvailable SinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
rTetR-NS3-VP64-p65_IRES_mCherry
Plasmid#112628PurposeEncodes the drug-activated transcription factor rTetR-NS3-VP64-p65 along with IRES mCherry as a fluorescent marker.DepositorInsertrTetR-NS3-VP64-p65
TagsAU1 (N term on NS3), HA (C term on NS3), and T7 (…ExpressionMammalianMutationNS3 mutant T54APromoterCMVAvailable SinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJT9R
Plasmid#98305PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of GAL1/GAL2 promoterDepositorInsertTRPL3AcNES1>TRPL41B
ExpressionYeastAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
MS2_adRNA-MCP_ADAR2 DD (E488Q)_NLS
Plasmid#124705PurposeExpresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD(E488Q)_NLSDepositorInsertMCP_ADAR2 DD (E488Q)_NLS
UseAAVTagsNLSExpressionMammalianMutationE488Q hyperactive mutantPromoterCMVAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ChRger1-TS-EYFP
Plasmid#127243PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.DepositorInsertChRger1-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianPromoterhSyn1Available SinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only