We narrowed to 12,110 results for: NSI
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Plasmid#68426PurposeTransient expression in mammalian cells of an "INT" construct_bearing three MS2 Stem-loops, targeting the GLuc reporter. U6 promoterDepositorInsertINT construct_bearing three MS2 Stem-loops
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterhuman U6Available SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(7AW)RS
Plasmid#207516PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 7-Aza-L-Tryptophan (7AW) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
ERroGFP_iE_C204Q_pCDNA3
Plasmid#48629PurposeMammalian expression vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 204 to Glutamine mutation (roGFP C147, E147b, C204Q)DepositorInsertERroGFP
TagsFLAGExpressionMammalianMutationinsertion E147B, C204QAvailable SinceOct. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
DD-DamWT-LMNB1-IRES2-mCherry
Plasmid#159601PurposeShield-1 inducible transient mammalian expression of Dam-LMNB1 for DamID, containing the wild-type allele of Dam.DepositorInsertDD-DamWT-LMNB1-IRES2-mCherry
ExpressionMammalianPromoterCMVAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGG-TurboID-3XNLS
Plasmid#209392PurposeTransiently expressing TurboID fused with 3X nuclear localization signal (NLS) in plantaDepositorInsert3XHA-TurboID-3XNLS
ExpressionPlantPromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable SinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPMVAd3
Plasmid#98298PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.DepositorInsertPRPL8B-ERG12-TNAT5-PSSB1-ERG8-TIDP1-PRPL3-MVD1-TIDP1-PYEF3-IDI1-TRPL15A
ExpressionYeastAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
CFP-GAI(1-172)
Plasmid#37309DepositorAvailable SinceJune 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAXEF-Rap1-E63(GTP)
Plasmid#32698DepositorInsertRAP1 (RAP1A Human)
ExpressionMammalianMutationE63: Consitutively GTP-boundPromoterEF promoterAvailable SinceDec. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ChRger3-TS-EYFP
Plasmid#127240PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.DepositorInsertChRger3-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianPromoterhSyn1Available SinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJFRC203-10XUAS-FRT>STOP>FRT-myr::smGFP-cMyc
Plasmid#63167PurposeGAL4-responsive UAS “flp-On” spaghetti monster GFP-cMyc reporterDepositorInsert"spaghetti monster GFP-cMyc"
TagsN-myristoylationExpressionInsectAvailable SinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTJV1Sc-rpoB
Plasmid#166978PurposeGenerates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistanceDepositorInsertsUseCRISPR and Synthetic BiologyExpressionBacterialPromoterJ23101 and VanCCAvailable SinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 2xFLAG-2xSTREP TFDP2
Plasmid#236445Purposetransient overexpression of TFDP2 in mammalian cellsDepositorAvailable SinceMay 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Plagl2
Plasmid#228061Purposetransiently express mouse Plagl2DepositorInsertPlagl2 (Plagl2 Mouse)
ExpressionMammalianAvailable SinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
FRB_LD 0_CS G_tTA-BFP
Plasmid#58876PurposeMESA target chain with Rapamycin FRB ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with FRB ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianPromoterCMVAvailable SinceNov. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJFRC208-10XUAS-FRT>STOP>FRT-myr::smGFP-FLAG
Plasmid#63169PurposeGAL4-responsive UAS “flp-On” spaghetti monster GFP-FLAG reporterDepositorInsert"spaghetti monster GFP-FLAG"
TagsN-myristoylationExpressionInsectAvailable SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTG06-Amp-FRT
Plasmid#128275PurposeDeg-LITer2.0 gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-RRRG-P2A-GFPDepositorInsertsTetR-LOV-RRRG
EGFP
UseSynthetic BiologyExpressionMammalianPromoterCMVAvailable SinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEN716 - pCAGGS-eGFP-3Xnls-bpA
Plasmid#156435PurposeFor transient expression of nuclear eGFP in mammalian cellsDepositorInserteGFP
ExpressionMammalianMutationnoneAvailable SinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lyn-mCherry-GAI(1-92)
Plasmid#37315DepositorInsertGAI (GAI Mustard Weed)
TagsLyn and mCherryExpressionMammalianMutationcontains aa 1-92PromoterCMVAvailable SinceJune 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-i3 G183S ONE-GO
Plasmid#204338PurposeGPCR/G protein BRET biosensorDepositorInsertGalpha-i3 G183S ONE-GO
UseLentiviralMutationG183S mutation in Galpha-i3 (renders it RGS insen…Available SinceJan. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
Burden Monitor phi80 version
Plasmid#66074PurposeThe burden monitor was cloned inside plasmid pAH153 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cellDepositorInsertGFP
Available SinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-ChRger2-TS-EYFP
Plasmid#127238PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.DepositorInsertChRger2-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianPromoterCamKIIaAvailable SinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB-CMV-MCS-puro PM-mKate2-P2A-GEM-LTB4mut
Plasmid#202644PurposeExpresses the LTB4-insensitive control sensor GEM-LTB4mut along with the plasma membrane targeted mKate2 tag in mammalian cellsDepositorInsertPM-mKate2-P2A-GEM-LTB4mut
TagsPM-mKate2ExpressionMammalianPromoterCMVAvailable SinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-CRY2(D387A)-mCherry
Plasmid#58369PurposeLight insensitive CRY2 mutantDepositorInsertCRY2[1-498] (D387A) (CRY2 Mustard Weed)
TagsmCherryExpressionMammalianMutationD387APromoterCMVAvailable SinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
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OSCA2.3
Plasmid#136595PurposeExpresses the mechanosensitive channel OSCA2.3 in mammalian cellsDepositorAvailable SinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFR-mScarletI-antiFRET-P2A-G-Ca-FLITS
Plasmid#191457PurposeCompare the intensity of the genetically encoded calcium sensor (GECI) G-Ca-FLITS to mScarlet-I fluorescent proteinDepositorInsertG-Ca-FLITS
TagsHis-mScarletI-antiFRETlinker-P2AExpressionBacterial and MammalianAvailable SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJT9R
Plasmid#98305PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of GAL1/GAL2 promoterDepositorInsertTRPL3AcNES1>TRPL41B
ExpressionYeastAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[BoBS]
Plasmid#68431PurposeTransient expression in mammalian cells of an "INT" construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct, targeting the GLuc reporter. U6 promoterDepositorInsertINT construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterhuman U6Available SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ABKAR
Plasmid#140051PurposeGenetically encoded FRET-based biosensor for monitoring AMPK activity. Also sensitive to phosphorylation by BRSK1/2 when expressed in neurons.DepositorInsertABKAR
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ChRger1-TS-EYFP
Plasmid#127243PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.DepositorInsertChRger1-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianPromoterhSyn1Available SinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXJ-Myc-FOXA1
Plasmid#232645Purposetransient expresses FOXA1 in mammalian cellsDepositorAvailable SinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-3XFLAG-renilla-luciferase-beta-globin-(39PTC)
Plasmid#184394PurposeTransient transfection plasmid expressing Renilla-luciferase-beta-globin fusion protein with PTC in beta-globinDepositorInsertRenilla luciferase
Tags3X FLAGExpressionMammalianPromoterCMVAvailable SinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMIR-His-Halo-Tev-PTEN
Plasmid#58241Purposeexpresses halo tagged PTEN proteinDepositorInsertphosphatase and tensin homolog (PTEN Human)
TagsHis-Halo-TevExpressionMammalianPromoterCMVAvailable SinceAug. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTEV5_ RpMatB_2
Plasmid#78206PurposeExpression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - tighter bindingDepositorInsertmatB (His6 C106A S170A R286C Q457C K488A)
TagsHis6ExpressionBacterialMutationC106A S170A R286C Q457C K488APromoterT7Available SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTUB1:YFP-AID-3HA, DHFR-TS:HXGPRT
Plasmid#87260PurposeYFP-AID-3HA fusion driven by a minimal TUB1 promoter with an HXGPRT drug selectable marker. The AID CDS is Arabidopsis thaliana auxin-responsive protein IAA17.DepositorInsertsYFP-AID-3HA
HXGPRT
UseToxoplasma expressionTags3HA and AIDPromoterTgDHFR-TS 463 bp and TgTUB1 378 bpAvailable SinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[3xPP7_SL]
Plasmid#68424PurposeTransient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U6 promoterDepositorInsertINT construct bearing three PP7 Stem-loops
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterhuman U6Available SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tom20-mCherry-GAI(1-92)
Plasmid#37316DepositorInsertGAI (GAI Mustard Weed)
TagsTom20 and mCherryExpressionMammalianMutationcontains aa 1-92PromoterCMVAvailable SinceJune 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-EGFP-AP4-mito
Plasmid#229693PurposeTransient expression of EGFP AP4-mito in mammalian cellsDepositorInsertEGFP; Control construct with tandem AP4 motifs (mitochondria)
TagsEGFPExpressionMammalianPromoterCMV promoterAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVenus-N-SLC35B2
Plasmid#155038PurposeTransient expression of SLC35B2-VenusDepositorInsertSLC35B2 (SLC35B2 Human)
TagsVenusExpressionMammalianMutationSee depositor comments belowPromoterCMVAvailable SinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-haloTyrRS C6
Plasmid#141174PurposeExpression of orthogonal tRNA and tRNA synthetase for incorporation of unnatural amino acidsDepositorInsertC6 HaloTyr tRNA synthetase
UseLentiviralExpressionMammalianMutationL270S, Y271L, N311G, C313TPromoterCMVAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only