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Plasmid#17882DepositorInsertBAF45c (Dpf3 Mouse)
UseTagsFLAG and IRES EGFPExpressionMammalianMutationPromoterAvailable sinceMay 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
BPK880-pCAG-DmrC-NLS-3xFLAG-VP64
Plasmid#136912PurposeDmrC with VP64 fused to it's C-terminus; Mammalian expression vectorDepositorInsertpCAG-DmrC-NLS-3xFLAG-VP64
UseCRISPRTagsExpressionMammalianMutationPromoterChicken Beta ActinAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pORANGE_NFL linker(A6TAG)-3xFLAG KI
Plasmid#182680PurposeExpression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker(A6TAG)-3xFLAG. Can be used for amber codon suppression and click chemistry labeling of endogenous NFL.DepositorInsertNefl-targeting gRNA and linker(A6TAG)-3xFLAG donor sequence (Nefl Mouse)
UseTagsExpressionMammalianMutationPromoterU6 and chicken beta-actin promoterAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHLmMBP-8
Plasmid#72347PurposeMammalian expression of cytoplasmic mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tagDepositorTypeEmpty backboneUseTags6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…ExpressionMammalianMutationPromoterCMV enhancer + chicken beta-actin promoterAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG R2pH-LAMP1-3xFLAG
Plasmid#157940Purposeexpresses ratiometric sensor of lysosomal lumenal pHDepositorInsertR2pH-LAMP1-3xFLAG (Lamp1 Mouse)
UseTags3XFLAG, mCherry, and pHluorinExpressionMammalianMutationPromoterChick beta actin pomoterAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBa-KIF5C 559-tdTomato-FKBP
Plasmid#64211PurposeKIF5C motor protein aa1-559 with C-terminal tandem dimer Tomato and FKBP tagsDepositorInsertKif5c (Kif5c Rat)
UseTagsFKBP and tdTomatoExpressionMammalianMutationmotor domain aa1-559Promoterchicken Beta ActinAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHLmMBP-11
Plasmid#72349PurposeMammalian expression of secreted mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tagDepositorTypeEmpty backboneUseTags6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…ExpressionMammalianMutationPromoterCMV enhancer + chicken beta-actin promoterAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
1179_pAAV-U6-BbsI-gRNA-CB-EmGFP
Plasmid#89060PurposeAAV-gRNA cloning vector with GFP reporterDepositorInsertEmGFP
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterChicken beta actin with partial CMV enhancerAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAX-mScarlet-FBP17
Plasmid#178366PurposeExpresses FBP17 c-terminal labeled with mScarelt in mammalian cellsDepositorInsertFormin Binding Protein 17 (FNBP1 Human)
UseTagsExpressionMammalianMutation400 Glutamic Acid changed to Aspartic acid (403 A…PromoterChicken Beta ActinAvailable sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHLmMBP-1
Plasmid#72343PurposeMammalian expression of secreted mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tagDepositorTypeEmpty backboneUseTags6His-tag, HA-tag, Strep-tag II, and mMBPExpressionMammalianMutationPromoterCMV enhancer + chicken beta-actin promoterAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBa-tdTomato-flag-BicD2 594-FKBP
Plasmid#64205PurposeBicaudal D protein aa1-594 (excluding start Met) with N-terminal tandem dimer Tomato and flag tags and C-terminal FKBPDepositorInsertBicaudal D protein (Bicd2 Mouse)
UseTagsFKBP, flag, and tdTomatoExpressionMammalianMutationno start methionine, aa1-594Promoterchicken Beta ActinAvailable sinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330-mitf
Plasmid#69801PurposeUsed to make mitf knockout porcine cell lineDepositorInsertsmitf-sgRNA
SpCas9
UseTagsExpressionMammalianMutationPromoterChicken Beta-Actin and U6Available sinceDec. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHLmMBP-3
Plasmid#72345PurposeMammalian expression of secreted N-terminally 8His-tagged mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tagDepositorTypeEmpty backboneUseTags6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…ExpressionMammalianMutationPromoterCMV enhancer + chicken beta-actin promoterAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHLmMBP-6
Plasmid#72346PurposeMammalian expression of cytoplasmic mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tagDepositorTypeEmpty backboneUseTags6His-tag, HA-tag, Strep-tag II, and mMBPExpressionMammalianMutationPromoterCMV enhancer + chicken beta-actin promoterAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBa-eGFP-flag-BicD2 594-FKBP
Plasmid#63569PurposeBicaudal D protein aa1-594 (excluding start Met) tagged with N-terminal eGFP and flag and a C-terminal FKBPDepositorInsertBicaudal D protein (Bicd2 Mouse)
UseTagsFKBP, eGFP, and flagExpressionMammalianMutationno start methionine, aa1-594Promoterchicken Beta ActinAvailable sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHLmMBP-2
Plasmid#72344PurposeMammalian expression of secreted N-terminally 6His-tagged mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tagDepositorTypeEmpty backboneUseTags6His-tag, HA-tag, Strep-tag II, and mMBPExpressionMammalianMutationPromoterCMV enhancer + chicken beta-actin promoterAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGBT-R16
Plasmid#31827DepositorInsertmini Tol2 ITR
UseCre/Lox; Gene trapTagsmRFPExpressionMutationmRFP lacks start codonPromoterbeta ActinAvailable sinceSept. 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCIG-FGFRdn
Plasmid#80431PurposeExpression of a dominant-negative FGFR1 with a nuclear EGFP reporter in chick embryosDepositorInsertFibroblast growth factor receptor 1 (FGFR1 Chicken)
UseTagsEGFPExpressionMammalianMutationcontains aa 1–425Promoterbeta-actin promoterAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
1183_pAAV-U6-Ex14-gRNAd-CB-EmGFP
Plasmid#89061PurposeAAV-gRNA targeting the murine Ldlr geneDepositorInsertEmGFP
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterChicken beta actin with partial CMV enhancerAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -