165,637 results

1. pLVX-Tight-Puro-KrasG12D2B

   Plasmid

   #139786

   Purpose

   Lentiviral tet-inducible expression of KrasG12D (mouse)

   Depositor

   Joan Massague

   Article

   Su et al Nature. 2020 Jan;577(7791):566-571. doi:

   Insert

   KrasG12D (Kras Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   G12D

   Promoter

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. MSCV-EGFP-Bulged-miR-19

   Plasmid

   #91976

   Purpose

   EGFP reporter cell line with eight bulged miR-19 sites

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   EGFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pICE-HA-MRE11-H63S

   Plasmid

   #82035

   Purpose

   Plasmid for constitutive or doxycycline-inducible expression of H63S mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

   Depositor

   Sébastien Britton, Patrick Calsou

   Article

   Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

   Insert

   MRE11 (MRE11 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   H63S MRE11 mutant

   Promoter

   CMV-tet

   Available since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-HA AKT1

   Plasmid

   #78778

   Purpose

   To overexpress AKT1 in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Lee et al Nat Commun. 2015 Jul 17;6:7769. doi: 10.

   Insert

   AKT1 (AKT1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJL 89

   Plasmid

   #98558

   Purpose

   Useful for making infectious clones of RNA virus cDNAs; Has 35S promoter and HDV Ribozyme sequences in T-DNA region of Agrobacterium vector.

   Depositor

   John Lindbo

   Article

   Lindbo et al Plant Physiol. 2007 Dec;145(4):1232-40.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Enh 35S

   Available since

   Aug. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ141D2.0

   Plasmid

   #99906

   Purpose

   Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   OsU3

   Available since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3β-FLAG-CBP-LD-HA

   Plasmid

   #32906

   Depositor

   Tso-Pang Yao

   Article

   Zhao et al Mol Cell Biol. 2005 Oct;25(19):8456-64.

   Insert

   C/EBP (Crebbp Mouse)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   L1435A/D1436A

   Promoter

   CMV

   Available since

   Nov. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGGF007

   Plasmid

   #48847

   Purpose

   Provides a kanamycin resistance cassette as GreenGate module.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Insert

   pNOS::KanamycinR::tNOS
   

   Use

   Golden gate compatible cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. TCARZ-CBh-GFP-SV40PA-GF3-R4A22

   Plasmid

   #203767

   Purpose

   For production of template RNA for use in PRINT, GFP under CBh promoter

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   PRINT template RNA for GFP
   

   Use

   Ivt template

   Tags

   Expression

   Mutation

   Promoter

   CBh

   Available since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVCT-tTR-KRAB

    Plasmid

    #11643

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

    Depositor

    Patrick Aebischer, Didier Trono

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    CAG promoters, GFP, tTR-KRAB, Tet-on
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GFP-itis Bacterial Base Editor Activity

    Plasmid Kit

    #1000000219

    Purpose

    This collection contains all plasmids needed for preparation and execution of the "GFP-itis" bacterial base editing activity.

    Depositor

    Alexis Komor

    Available since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3-Flag-IDH1-R132H

    Plasmid

    #62907

    Purpose

    Expresses R132 mutant IDH1 in mammalian cells

    Depositor

    Yue Xiong

    Article

    Zhao et al Science. 2009 Apr 10;324(5924):261-5. do

    Insert

    IDH1 (IDH1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deleted Arg132 (R132)

    Promoter

    Available since

    April 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcs2-GFP-IRES-mCherry

    Plasmid

    #226098

    Purpose

    Protein stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.

    Depositor

    Michael Rape

    Article

    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. MGAT2-RpHLuorin2

    Plasmid

    #171718

    Purpose

    Cis-/medial-Golgi expression of ratiometric pHLuorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2 fused to MGAT2 (MGAT2 Human)
    

    Use

    Tags

    ratiometric pHluorin2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1a-DIO-mGFP-MutCREB(S133A)

    Plasmid

    #194642

    Purpose

    Expresses the fusion of monomeric-EGFP-tagged mutant CREB(S133A) in a Cre-dependent manner

    Depositor

    Jun-Hyeong Cho

    Article

    Lee et al Nat Neurosci. 2022 Dec 23. doi: 10.1038/

    Insert

    CREB (Creb1 Rat)
    

    Use

    AAV

    Tags

    mEGFP

    Expression

    Mutation

    S133A

    Promoter

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUCHR-osTIR1-IRES-GFP

    Plasmid

    #110661

    Purpose

    Lentiviral vector for stable expression of osTIR1 and selection with GFP

    Depositor

    Dmitriy Mazurov

    Article

    Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

    Insert

    osTIR1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TFORF1665

    Plasmid

    #141863

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TP73 (TP73 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 5xGAL4-TATA-luciferase

    Plasmid

    #46756

    Purpose

    Luciferase reporter gene for factors with GAL4 DNA binding domains

    Depositor

    Richard Maurer

    Article

    Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

    Insert

    Luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    minimal TATA

    Available since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-BRCA1

    Plasmid

    #119007

    Purpose

    Mammalian expression of human BRCA1 and BFP

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pscALPS- SIV-MAC251 vpx

    Plasmid

    #115810

    Purpose

    Encodes codon optimized 3xFLAG-VpxMAC251 and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVMAC251 Vpx
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pComb3XLambda

    Plasmid

    #63892

    Purpose

    Backbone pComb3X containing PCR template for human antibody lambda light chain constant region

    Depositor

    Carlos Barbas

    Article

    Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

    Insert

    human antibody lambda light chain (IGLL5 Human)
    

    Use

    Phage display

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacZ

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. EC70188

    Plasmid

    #209449

    Purpose

    Cloning protospacers for Cas9 editing

    Depositor

    Cristobal Uauy, Wendy Harwood

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    Cas9 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    HvU3

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRK5-HA-Ubiquitin-K11

    Plasmid

    #22901

    Depositor

    Sandra Weller

    Article

    Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K11 only, all other lysines mutated to arginines

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. hRad51(A89E)–Cas9(D10A)

    Plasmid

    #125563

    Purpose

    Expresses the Rad51(A89E)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

    Insert

    Rad51(A89E)–Cas9(D10A) (RAD51 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJL056

    Plasmid

    #177168

    Purpose

    Expresses wildtype MRE11 in human cells

    Depositor

    Brittany Adamson

    Article

    Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

    Insert

    MRE11 (MRE11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TRE3G-PE2-P2A-BFP

    Plasmid

    #231580

    Purpose

    Expresses the PE2 prime-editing machinery fused to BFP (PE2-P2A-BFP), under the control of a TRE3G promoter. This promoter is responsive to doxycycline bound to the rtTA protein

    Depositor

    Jesse Engreitz

    Article

    Martyn et al bioRxiv [Preprint]. 2023 Dec 21:2023.12.

    Insert

    PE2-P2A-BFP
    

    Use

    Lentiviral

    Tags

    P2A-BFP

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEGFPC1-FLAG-Synaptojanin 1-170

    Plasmid

    #22294

    Depositor

    Pietro De Camilli

    Article

    Perera et al Proc Natl Acad Sci U S A. 2006 Dec 19. 1

    Insert

    Synaptojanin 1_170 (SYNJ1 Human)
    

    Use

    Tags

    Flag and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

28. TDP-43_CTD_M337V

    Plasmid

    #98674

    Purpose

    Expresses 6xHis-tagged C-terminal domain of TDP-43 with M337V

    Depositor

    Nicolas Fawzi

    Article

    Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M337V

    Promoter

    Available since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pS24·Pm-GFP

    Plasmid

    #122594

    Purpose

    Expresses GFP under the regulatory control of Pm. Expression needs additional xyls gene

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

    Insert

    Pm promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pm

    Available since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-CamKII-ArchT-GFP (PV2527) (AAV1)

    Viral Prep

    #99039-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-CamKII-ArchT-GFP (PV2527) (#99039). In addition to the viral particles, you will also receive purified pAAV-CamKII-ArchT-GFP (PV2527) plasmid DNA. CaMKII-driven ArchT-GFP expression for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Article

    Han et al Front Syst Neurosci. 2011;5:18. Epub 201

    Promoter

    CaMKII

    Tags

    GFP

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

31. pXJ-Myc-IRF1

    Plasmid

    #232641

    Purpose

    transient expresses IRF1 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al NPJ Genom Med. 2022 Jan 19;7(1):4. doi:

    Insert

    IRF1 (IRF1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBK-YEE-BE3

    Plasmid

    #85177

    Purpose

    Expresses YEE-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    YEE-BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pC1-SypHer3s-IMS

    Plasmid

    #108120

    Purpose

    Mitochondrial ratiometric pH probe with enhanced brightness fused to Smac/DIABLO fragment. Probe is localized in mitochondrial intermembrane space. Mammalian cell expression.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

    Insert

    SypHer3s-IMS
    

    Use

    Tags

    ratiometricYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. PyronicSF/pcDNA3.1(-)

    Plasmid

    #124812

    Purpose

    Highly resposive GFP-based pyruvate nanosensor.

    Depositor

    Luis Felipe Barros

    Article

    Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

    Insert

    PyronicSF
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. p4489 Flag-betaTrCP

    Plasmid

    #10865

    Depositor

    Peter Howley

    Article

    Zhou et al Mol Cell. 2000 Sep . 6(3):751-6.

    Insert

    betaTrCP (BTRC Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. mApple-TOMM20-N-10

    Plasmid

    #54955

    Purpose

    Localization: Outer Mitochondrial Membrane, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Insert

    TOMM20 (TOMM20 Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. TFORF2069

    Plasmid

    #141902

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. p13xQUAS-MCS, α-cry:mCherry

    Plasmid

    #180481

    Purpose

    Inducible gene expression vector p13xQUAS-MCS, α-cry:mCherry

    Depositor

    Hyunju Ro

    Article

    Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

    Insert

    Multiple cloning site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pExp-GB1

    Plasmid

    #112565

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GB1 fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-GB1 and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA5-puro-eGFP-HEC1

    Plasmid

    #114022

    Purpose

    Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    eGFP-HEC1 - Wildtype (NDC80) (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge