We narrowed to 13,844 results for: cas9 genes

1. pRExT2A-CT-BTy

   Plasmid

   #232209

   Purpose

   Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: 3xTy-T2A-BSD-3xTy

   Depositor

   Calvin Tiengwe

   Article

   Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

   Insert

   3xTy
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRExT2A-NT-PTy

   Plasmid

   #232202

   Purpose

   Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy

   Depositor

   Calvin Tiengwe

   Article

   Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

   Insert

   3xTy
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRExT2A-NT-PmSc

   Plasmid

   #232548

   Purpose

   Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy-mScarlet

   Depositor

   Calvin Tiengwe

   Article

   Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

   Insert

   mScarlet
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. sgPBRM1-1- LentiCRISPRv2

   Plasmid

   #107403

   Purpose

   Lentiviral expression of Cas9 and gRNA targeting PBRM1

   Depositor

   William Kaelin

   Article

   Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

   Insert

   gRNA targeting PBRM1 (PBRM1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. sgPBRM1-2- LentiCRISPRv2

   Plasmid

   #107404

   Purpose

   Lentiviral expression of Cas9 and gRNA targeting PBRM1

   Depositor

   William Kaelin

   Article

   Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

   Insert

   gRNA targeting PBRM1 (PBRM1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PB-UniSAM

   Plasmid

   #99866

   Purpose

   Encodes for Cas9-VP64, MS2-p65-HSF1, mCherry and for the gRNA 2.0

   Depositor

   Lesley Forrester

   Article

   Fidanza et al Sci Rep. 2017 Jul 25;7(1):6394. doi: 10.

   Insert

   UniSAM-mCherry + U6-gRNA2.0
   

   Use

   CRISPR and Synthetic Biology; Dcas9-sam activation

   Tags

   Expression

   Mammalian

   Mutation

   BbsI sites in CDS were ablated by consensus mutat…

   Promoter

   EF1a

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pG3H-PE3max-attR1R2

   Plasmid

   #213049

   Purpose

   Destination vector Expressing nCas9 and RT fusion protein and carrying Gateway recombination sites

   Depositor

   Bing Yang

   Article

   Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

   Inserts

   Cas9 nickase
   M-MLV Reverse Transcriptase
   

   Use

   Note: this plasmid needs helper pvs1-vir2 for rep…

   Tags

   Expression

   Plant

   Mutation

   H840A, R240K, N413K,

   Promoter

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLentiCRISPR-v1-sgHK2_2

   Plasmid

   #163461

   Purpose

   lentiviral vector expressing Cas9 and an sgRNA targeting HK2

   Depositor

   Jason Cantor

   Article

   Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

   Insert

   sgRNA 2 targeting HK2 (HK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLY152

   Plasmid

   #130950

   Purpose

   dcas9 generator and reporter circuit (PpspA-2G6 with sfgfp::ASV)

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Inserts

   dcas9
   tetR
   sfgfp
   

   Use

   Synthetic Biology

   Tags

   ASV tag

   Expression

   Bacterial

   Mutation

   Mutations D10A and H840A on WT cas9. Synonymous m…

   Promoter

   PpspA-2G6 and Ptet

   Available since

   Sept. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. gRNA giantin/GOLGB1

    Plasmid

    #222315

    Purpose

    CRISPR/Cas9 close to the ATG of giantin/GOLGB1 gene.

    Depositor

    Franck Perez

    Article

    Fourriere et al J Cell Sci. 2016 Sep 1;129(17):3238-50.

    Insert

    gRNA targeting GOLB1 (GOLGB1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUt-mNF-Cas13d

    Plasmid

    #224789

    Purpose

    LPUtopia matching RMCE donor plasmid with mCherry-P2A-RfxCas13d Negative-Feedback circuit. Use BlastR for positive selection and HSV-TK for negative selection.

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    mCherry-P2A-RfxCas13d
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-d2i

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTij_sg_mmu_Med6_C_terminal

    Plasmid

    #197869

    Purpose

    CRISPR/Cas9/sgRNA plasmid for cutting Med6 C terminal and building MED6-mEmerald/Halo knock-in mESCs

    Depositor

    James Zhe Liu

    Article

    Dong et al bioRxiv 2022.10.26.513917

    Insert

    Med6 (Med6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSLiP-G1

    Plasmid

    #188963

    Purpose

    L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown

    Depositor

    Seung-Goo Lee

    Article

    Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

    Inserts

    dCas9
    sgRNA: atcggtcgcattgttttccactagg
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PrhaBAD

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. sgMTOR_49

    Plasmid

    #125132

    Purpose

    Targeting 49th exon of human MLST8 gene by CRISPR-Cas9

    Depositor

    Jin Chen

    Article

    Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

    Insert

    MTOR (MTOR Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSLiP-G1G2

    Plasmid

    #188965

    Purpose

    L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown

    Depositor

    Seung-Goo Lee

    Article

    Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

    Inserts

    dCas9
    sgRNA 1: atcggtcgcattgttttccactagg, sgRNA 2: gttagacgctgattacatggactagg
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PrhaBAD

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSLiP-G2

    Plasmid

    #188964

    Purpose

    L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown

    Depositor

    Seung-Goo Lee

    Article

    Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

    Inserts

    dCas9
    sgRNA: gttagacgctgattacatggactagg
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PrhaBAD

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pX459-dEGF-5’UTR

    Plasmid

    #177260

    Purpose

    A knockout vector for the dog Egf

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    A gRNA targeting the dog Egf gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LentiCRISPRv2-neo-dTgfa

    Plasmid

    #173842

    Purpose

    A knockout vector for the dog Tgfa.

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    A gRNA targeting the dog Tgfa gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LentiCRISPRv2-puro-dEgfr

    Plasmid

    #173844

    Purpose

    A knockout vector for the dog Egfr.

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    A gRNA targeting the dog Egfr gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX459-dEgf

    Plasmid

    #173700

    Purpose

    A knock-out vector for the dog Egf

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    A gRNA targeting the dog Egf gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pROS13_IDH2 #7

    Plasmid

    #166102

    Purpose

    This plasmid express a sgRNA that targets the IDH2 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH2-135 by homologous recombination.

    Depositor

    Matthias Heinemann

    Article

    Chen et al bioRxiv

    Insert

    IDH2 sgRNA #7 (IDH2 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pROS13_IDH1 #4

    Plasmid

    #166101

    Purpose

    This plasmid express a sgRNA that targets the IDH1 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH1-67 by homologous recombination.

    Depositor

    Matthias Heinemann

    Article

    Chen et al bioRxiv

    Insert

    IDH1 sgRNA #4 (IDH1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDGE347

    Plasmid

    #153228

    Purpose

    Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

    Depositor

    Sylvestre Marillonnet

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJRH-1346 U6-B2M sgRNA Gag-pol v2

    Plasmid

    #201917

    Purpose

    Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Inserts

    Gag-pol
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and Human U6

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDGE651

    Plasmid

    #153229

    Purpose

    Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

    Depositor

    Sylvestre Marillonnet

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. px459-TDP-43 sgRNA1

    Plasmid

    #133762

    Purpose

    Expresses Cas9 and human TDP-43 sgRNA

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. px459-TDP 43 sgRNA2

    Plasmid

    #133763

    Purpose

    Expresses Cas9 and human TDP-43 sgRNA

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLentiCRISPRv1 sgTollip

    Plasmid

    #196546

    Purpose

    Lentiviral CRISPR-Cas9 plasmid containing gRNA targeting exon 1 of human Tollip. Used for generation of Tollip protein knockouts in human cell lines.

    Depositor

    David Tumbarello

    Article

    Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

    Insert

    Toll-Interacting Protein (TOLLIP Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. BPK1179

    Plasmid

    #179296

    Purpose

    Expresses dCas9 fused to four DmrA domains

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

    Insert

    dCas9-DmrA(x4)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    CAG

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDGE666

    Plasmid

    #153231

    Purpose

    Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

    Depositor

    Sylvestre Marillonnet

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    GFP

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. dCBE4-gam

    Plasmid

    #124449

    Purpose

    Expresses dead Cas9 (dCas9)-CBE4-gam

    Depositor

    George Church

    Article

    Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

    Insert

    dCBE4-gam
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Available since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. PXPR007 sgGFP-1

    Plasmid

    #74960

    Purpose

    Cas9 + sgGFP-1 with blasticidin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    GFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDGE668

    Plasmid

    #153233

    Purpose

    Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

    Depositor

    Sylvestre Marillonnet

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    GFP

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDGE667

    Plasmid

    #153232

    Purpose

    Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

    Depositor

    Sylvestre Marillonnet

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    GFP

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBTK615

    Plasmid

    #110609

    Purpose

    BTK assembled plasmid - used in Stage 2 assembly to target Cas9 or dCas9

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    sgRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDGE652

    Plasmid

    #153230

    Purpose

    Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

    Depositor

    Sylvestre Marillonnet

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3.1_pCMV-nCas-PmCDA1 pH1-gRNA(HPRT)

    Plasmid

    #79619

    Purpose

    Expresses nCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    3xFlag-PmCDA1

    Expression

    Mammalian

    Mutation

    D10A for nickase Cas9

    Promoter

    pCMV

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3.1_pCMV-dCas-PmCDA1 pH1-gRNA(HPRT)

    Plasmid

    #79621

    Purpose

    Expresses dCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Mammalian

    Mutation

    D10A and H840A for nuclease deficient Cas9

    Promoter

    pCMV

    Available since

    Sept. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. PXPR007 sgGFP-2

    Plasmid

    #74961

    Purpose

    Cas9 + sgGFP-2 with blasticidin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    GFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pJT131_GalL_cCDA1-NGBE3

    Plasmid

    #145095

    Purpose

    Expressing base editor cCDA1-NGBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    cCDA1-NGBE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…

    Promoter

    GalL

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge