We narrowed to 12,357 results for: NUC

1. pRS315e_pGal-dCas9-SH3

   Plasmid

   #79613

   Purpose

   Expresses dCas9-SH3 in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Use

   Tags

   SH3 domain

   Expression

   Yeast

   Mutation

   D10A and H840A for nuclease deficient Cas9

   Promoter

   pGal1

   Available since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. NET39 mRFP-C1 (1219)

   Plasmid

   #62042

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   NET39 (PPAPDC3 Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pAcGFP-Jpx9C104A

   Plasmid

   #120380

   Purpose

   Reporter for RNA nuclear localization

   Depositor

   Igor Ulitsky

   Article

   Lubelsky et al Nature. 2018 Mar 1;555(7694):107-111. do

   Insert

   JPX (JPX Human)
   

   Use

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   C321A

   Promoter

   CMV

   Available since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pOPTXLUC

   Plasmid

   #68504

   Purpose

   cyclic nucleotide reporter for bacteria or plant cells

   Depositor

   Helen Irving

   Article

   Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

   Insert

   OPTX promoter (AT1G33440 Mustard Weed)
   

   Use

   Tags

   luciferase

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   OPTX

   Available since

   Sept. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET-28c-F30-2xdBroccoli-47CAG

   Plasmid

   #237217

   Purpose

   Express F30-2xdBroccoli tagged 47 repeats of CAG RNA in bacteria.

   Depositor

   Mingxu You

   Article

   Xue et al Nucleic Acids Res. 2023 Sep 8;51(16):833

   Insert

   47 CAG trinucleotide repeats RNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MLKS2ec

   Plasmid

   #130855

   Purpose

   Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology

   Depositor

   Hank Bass

   Article

   Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10

   Insert

   MLKS2
   

   Use

   Gateway entry vector

   Tags

   eGFP-FLAG-HA

   Expression

   Mutation

   Promoter

   Available since

   Sept. 28, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. MLKS2exp

   Plasmid

   #130856

   Purpose

   Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology

   Depositor

   Hank Bass

   Article

   Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10

   Insert

   MLKS2
   

   Use

   Tags

   eGFP-FLAG-HA

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 28, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pNZ18-CAH

   Plasmid

   #203159

   Purpose

   Replicates in E. coli, S. cerevisiae, and A. laidlawii

   Depositor

   Bogumil Karas

   Article

   Nucifora et al ACS Synth Biol. 2024 Jan 19;13(1):45-53.

   Insert

   Yeast centromere, autonomously replicating sequence, histidine auxotrophic marker
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pENmRFPCNAL2 pc1054

   Plasmid

   #167567

   Purpose

   The fusion protein contains a SV40 NLS followed by mRFP, linker and human PCNA.

   Depositor

   Cristina Cardoso

   Article

   Sporbert et al Nucleic Acids Res. 2005 Jun 21;33(11):35

   Insert

   Proliferating Cell Nuclear Antigen (PCNA Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET23PI-Sce ESARC D218A

    Plasmid

    #195608

    Purpose

    Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the D218A active site mutation

    Depositor

    Frederick Gimble

    Article

    Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

    Insert

    PI-SceI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T928C, T929C, A942C

    Promoter

    T7

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET23PI-Sce ESARC

    Plasmid

    #195607

    Purpose

    Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter

    Depositor

    Frederick Gimble

    Article

    Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

    Insert

    PI-SceI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C552T,C554A, A557T,G584T, C588A, G878T, C881T, C9…

    Promoter

    T7

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGP8GF-ZmAFB2/3 b2

    Plasmid

    #188425

    Purpose

    Zea Mays Nuclear Auxin Signaling Receptor AFB2/3 b2

    Depositor

    Britney Moss

    Article

    Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

    Insert

    Auxin Signaling Receptor AFB2/3 b2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGP8GF-ZmAFB2/3 a

    Plasmid

    #188423

    Purpose

    Zea Mays Nuclear Auxin Signaling Receptor AFB2/3 A

    Depositor

    Britney Moss

    Article

    Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

    Insert

    Auxin Signaling Receptor AFB2/3 a
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGP4G-REL2N91-ccdB

    Plasmid

    #188444

    Purpose

    Zea Mays Nuclear Auxin Signaling CoRepressor REL2N91 and pDEST

    Depositor

    Britney Moss

    Article

    Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

    Insert

    REL2N91/ccdB
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pENTR-ZmIAA8ΔPB1

    Plasmid

    #188432

    Purpose

    Zea Mays Nuclear Auxin Signaling Repressor IAA8

    Depositor

    Britney Moss

    Article

    Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

    Insert

    Auxin Signaling Repressor IAA8deltaPB1
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    deleted PB1 domain

    Promoter

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. KBB441

    Plasmid

    #185131

    Purpose

    Swi6 full length with L->A mutated in nuclear localization signal

    Depositor

    Ken Belanger

    Article

    Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

    Insert

    SWI6
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    SWI6 deletion of amino acids 450-458

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. KBB394

    Plasmid

    #185086

    Purpose

    NMA111 with both nuclear localization signals mutated to alanines fused to GFP (mutagenesis of KBB280)

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Nma111 KKR 9-11 AAA; KRK 28-30 AAA

    Promoter

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-T3-HypaCAS9-pA

    Plasmid

    #131467

    Purpose

    Expresses HypaCas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

    Depositor

    Wataru Fujii

    Article

    Ikeda et al Commun Biol. 2019 Oct 10;2:371. doi: 10.

    Insert

    Codon optimized HypaCas9
    

    Use

    CRISPR

    Tags

    Flag, NLS, and polyA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJK278

    Plasmid

    #71134

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H89N mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-89 to asparagine; additional N-…

    Promoter

    T7

    Available since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJK320

    Plasmid

    #71144

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H59S mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-59 to serine; additional N-term…

    Promoter

    T7

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJK319

    Plasmid

    #71143

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H59A mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-59 to alanine; additional N-ter…

    Promoter

    T7

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJK318

    Plasmid

    #71142

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H59Q mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-59 to glutamine; additional N-t…

    Promoter

    T7

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJK315

    Plasmid

    #71141

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H89G mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-89 to glycine; additional N-ter…

    Promoter

    T7

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJK314

    Plasmid

    #71140

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H89V mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-89 to valine; additional N-term…

    Promoter

    T7

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJK279

    Plasmid

    #71135

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H89D mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-89 to aspartate; additional N-t…

    Promoter

    T7

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJK283

    Plasmid

    #71139

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H59N mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-59 to asparagine; additional N-…

    Promoter

    T7

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJK282

    Plasmid

    #71138

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H59D mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-59 to aspartate; additional N-t…

    Promoter

    T7

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJK281

    Plasmid

    #71137

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H59F mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-59 to phenylalanine; additional…

    Promoter

    T7

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJK280

    Plasmid

    #71136

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H89F mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-89 to phenylalanine; additional…

    Promoter

    T7

    Available since

    Jan. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAG-AsCas12a-Plus-mCherry

    Plasmid

    #191231

    Purpose

    To express the AsCas12a nuclease variant "AsCas12a-Plus" that enhanced the editing activity and specificity

    Depositor

    Zhili Rong

    Article

    Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

    Insert

    AsCas12a-Plus-P2A-mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    E174R, R951K, R955A

    Promoter

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDN77

    Plasmid

    #61347

    Purpose

    mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/IkBα NES)

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

    Insert

    mCherry
    

    Use

    Tags

    GS linker-AsLov2-biNLS2 and NES IkBa

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pEGFP-C1-KSENB-Nup133

    Plasmid

    #87340

    Purpose

    To express human Nup133 in mammalian cells

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    NUP133 (NUP133 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deletion of Nup133 AA1-7

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. T7-RelA

    Plasmid

    #21984

    Depositor

    Warner Greene

    Article

    Chen Lf et al Science. 2001 Aug 31. 293(5535):1653-7.

    Insert

    NFkB p65 subunit (RELA Human)
    

    Use

    Tags

    T7 gp10

    Expression

    Mammalian

    Mutation

    For more details for this expression construct,…

    Promoter

    Available since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

34. pCLASP_mScarlet_CLASP_RGS2membrane

    Plasmid

    #133086

    Purpose

    Plasmid contains mScarlet with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-mScarlet-yeLANS). CLASP modulates nuclear localization of mScarlet in response to bl

    Depositor

    Hana El-Samad

    Article

    Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

    Insert

    mScarlet-CLASP
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. His-AgdNK

    Plasmid

    #203226

    Purpose

    For protein expression. A. gambiae deoxynucleoside kinase. Phosphorylates a wide variety of nucleosides at the 5-hydroxyl position. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

    Depositor

    Vern Schramm

    Article

    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

    Insert

    deoxynucleoside kinase (AgaP_AGAP001585 Anopheles gambiae)
    

    Use

    Tags

    N-terminal TEV cleavable 6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET28-SaCas9

    Plasmid

    #178901

    Purpose

    SaCas9 (Cas9 from Staphylococcus aureus)

    Depositor

    Hendrik Milting

    Article

    Flottmann et al STAR Protoc. 2022 Apr 5;3(2):101276. doi

    Insert

    SaCas9
    

    Use

    Tags

    His6-Tag, bipartite nuclear localization signal f…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. WN10151

    Plasmid

    #80441

    Purpose

    Expresses human codon-optimized dead (832A) LbCpf1

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Insert

    dead (D832A) LbCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEGFP-C1-Nup35-mut

    Plasmid

    #87341

    Purpose

    To express human Nup35 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    NUP35 (NUP35 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    shRNA resistant changes

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. iSpyMac

    Plasmid

    #155014

    Purpose

    Expresses the codon-optimized, engineered iSpyMac Cas9 nuclease in mammalian cells

    Depositor

    Joseph Jacobson

    Article

    Chatterjee et al Nat Commun. 2020 May 18;11(1):2474. doi:

    Insert

    iSpyMac
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    PID grafted onto SpyCas9 N-terminal domain, R221K…

    Promoter

    EF1ɑ

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRFP-NLS

    Plasmid

    #105296

    Purpose

    To visualize nuclear dynamics in living cells

    Depositor

    Kenneth Yamada

    Article

    Petrie et al Science. 2014 Aug 29;345(6200):1062-5. d

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    aa encoding 172-192

    Promoter

    Available since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pK7WGF2::hCas9

    Plasmid

    #46965

    Purpose

    Expesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissue

    Depositor

    Sophien Kamoun

    Article

    Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

    Insert

    hCas9
    

    Use

    CRISPR; Plant expression

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    35S

    Available since

    Aug. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pDN34

    Plasmid

    #61343

    Purpose

    mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/PKIt NES)

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

    Insert

    mCherry
    

    Use

    Tags

    GS linker-AsLov2-biNLS2 and PKIt NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pQCXIZ-turq-3xFLAG-TREX1 D18N

    Plasmid

    #164233

    Purpose

    retroviral plasmid for turq-3xFLAG-TREX1 D18N (catalytically-deficient)

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    TREX1 (TREX1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and mturquoise

    Expression

    Mammalian

    Mutation

    D18N ->nuclease deficient

    Promoter

    CMV

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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44. pAcrVIB1

    Plasmid

    #184566

    Purpose

    Inducible expression of anti-CRISPR protein AcrVIB1 in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    anti-CRISPR protein inhibiting Cas13b nucleases
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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45. BA31_pU.CAG.SaCas9-D10A.rBGpA

    Plasmid

    #199251

    Purpose

    Expression construct encoding the S. aureus Cas9.D10A nickase (SaCas9.D10A)

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Insert

    SaCas9.D10A nickase
    

    Use

    CRISPR

    Tags

    3XHA epitope, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CAG promoter

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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46. NET29A.Hs pEGFP-N2 (1181)

    Plasmid

    #62040

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

    Insert

    NET29A (TMEM120A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Splice variant

    Promoter

    CMV

    Available since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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47. pFRT/TO/FLAG/HA-DEST TNRC6B

    Plasmid

    #19884

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Oct 31. ():.

    Insert

    trinucleotide repeat containing 6B (TNRC6B Human)
    

    Use

    Tags

    FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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48. pSJ1321 (pRS315-NOP1pr-GFP11-mCherry-PUS1)

    Plasmid

    #86413

    Purpose

    nuclear split GFP11 reporter with mCherry

    Depositor

    Sue Jaspersen

    Article

    Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

    Insert

    Nop1pr-GFP11-mCherry-PUS1
    

    Use

    Tags

    GFP11 and mCherry

    Expression

    Yeast

    Mutation

    Promoter

    NOP1

    Available since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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49. pSFFV-dNme2Cas9

    Plasmid

    #193190

    Purpose

    Lentiviral vector expressing nuclease-dead Cas9 from Neisseria meningitis (dNme2)

    Depositor

    Erik Sontheimer

    Article

    Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

    Insert

    dNme2Cas9
    

    Use

    Tags

    NLS and NLS-3xHA-NLS

    Expression

    Mammalian

    Mutation

    H588A, D16A

    Promoter

    SFFV

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. pTriEx-mCherry::LANS4

    Plasmid

    #60785

    Purpose

    Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation

    Depositor

    Brian Kuhlman

    Article

    Yumerefendi et al PLoS One. 2015 Jun 17;10(6):e0128443. do

    Insert

    LANS4
    

    Use

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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