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FLAG-HA-pcDNA3.1-

Plasmid

#52535

Purpose

enables eukaryotic expression of N-terminally FLAG-HA-tagged fusion proteins

Depositor

Adam Antebi

Publication

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Type

Empty backbone

Tags

FLAG-HA

Expression

Mammalian

Promoter

CMV

Available since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

70 Articles

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FLAG-HA-pcDNA3.1-

Plasmid

#52535

Purpose

enables eukaryotic expression of N-terminally FLAG-HA-tagged fusion proteins

Depositor

Adam Antebi

Publication

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Type

Empty backbone

Tags

FLAG-HA

Expression

Mammalian

Promoter

CMV

Available since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

70 Articles

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pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP

Plasmid

#71237

Purpose

Express sgRNA and dCas9-KRAB from 3rd generation lentiviral vector

Depositor

Charles Gersbach

Publication

Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

Inserts

humanized dCas9-KRAB T2A GFP
sgRNA

Use

CRISPR and Lentiviral

Tags

Flag

Mutation

D10A and H840A

Available since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

69 Articles

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pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP

Plasmid

#71237

Purpose

Express sgRNA and dCas9-KRAB from 3rd generation lentiviral vector

Depositor

Charles Gersbach

Publication

Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

Inserts

humanized dCas9-KRAB T2A GFP
sgRNA

Use

CRISPR and Lentiviral

Tags

Flag

Mutation

D10A and H840A

Available since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

69 Articles

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pEGFP-C1-hApg5

Plasmid

#22952

Depositor

Noboru Mizushima

Publication

Mizushima et al J Biol Chem. 1998 Dec 18. 273(51):33889-

Insert

Homo sapiens ATG5 autophagy related 5 homolog (S. cerevisiae) (ATG5 Human)

Tags

EGFP

Expression

Mammalian

Available since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pMXs-Oct3/4

Plasmid

#13366

Purpose

Retroviral expression of mouse Oct3/4

Depositor

Shinya Yamanaka

Publication

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

POU domain, class 5, transcription factor 1 (Pou5f1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

83 Articles

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pMXs-Oct3/4

Plasmid

#13366

Purpose

Retroviral expression of mouse Oct3/4

Depositor

Shinya Yamanaka

Publication

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

POU domain, class 5, transcription factor 1 (Pou5f1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

83 Articles

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pXR002: EF1a-dCasRx-2A-EGFP

Plasmid

#109050

Purpose

Catalytically inactive dCasRx with 2A-EGFP

Depositor

Patrick Hsu

Publication

Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

Insert

dCasRx

Use

CRISPR and Lentiviral

Tags

2A-eGFP, HA, and NLS

Expression

Mammalian

Mutation

R239A/H244A/R858A/H863A

Available since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

27 Articles

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pXR002: EF1a-dCasRx-2A-EGFP

Plasmid

#109050

Purpose

Catalytically inactive dCasRx with 2A-EGFP

Depositor

Patrick Hsu

Publication

Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

Insert

dCasRx

Use

CRISPR and Lentiviral

Tags

2A-eGFP, HA, and NLS

Expression

Mammalian

Mutation

R239A/H244A/R858A/H863A

Available since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

27 Articles

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pShuttle-CMV

Plasmid

#16403

Purpose

Shuttle vector for use in AdEasy System. Used for expression of transgenes under a CMV promoter when no GFP tracer is desired.

Depositor

Bert Vogelstein

Publication

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Type

Empty backbone

Use

Adenoviral

Expression

Mammalian

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

24 Articles

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sgRNA(MS2) cloning backbone

Plasmid

#61424

Purpose

sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

Depositor

Feng Zhang

Publication

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Dec. 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

66 Articles

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sgRNA(MS2) cloning backbone

Plasmid

#61424

Purpose

sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

Depositor

Feng Zhang

Publication

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Dec. 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

66 Articles

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HA-p62

Plasmid

#28027

Purpose

Mammalian expression of HA tagged human p62

Depositor

Qing Zhong

Publication

Fan et al Autophagy. 2010 Jul 22. 6(5):.

Insert

p62 (SQSTM1 Human)

Tags

HA

Expression

Mammalian

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

57 Articles

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HA-p62

Plasmid

#28027

Purpose

Mammalian expression of HA tagged human p62

Depositor

Qing Zhong

Publication

Fan et al Autophagy. 2010 Jul 22. 6(5):.

Insert

p62 (SQSTM1 Human)

Tags

HA

Expression

Mammalian

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

57 Articles

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BJ5183 cells

Bacterial strain

#16398

Depositor

Bert Vogelstein

Publication

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Bacterial resistance

Streptomycin

Species

Escherichia coli

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pCAG-ERT2CreERT2

Plasmid

#13777

Purpose

Mammalian expression of conditionally active form of Cre recombinase (activated in response to tamoxifen)

Depositor

Connie Cepko

Publication

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

ERT2-Cre-ERT2 fusion protein

Use

Cre/Lox

Expression

Mammalian

Promoter

CAG

Available since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

62 Articles

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pUC18-mini-Tn7T-Plac-dCas9 (Plac)

Plasmid

#105235

Purpose

Tn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Plac promoter for expression in P. aeruginosa

Depositor

Kristala Prather

Publication

Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

Insert

S. pasteurianus dCas9

Use

CRISPR and Synthetic Biology

Tags

HA

Expression

Bacterial

Mutation

D10A, H599A

Available since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pBad-CA

Plasmid

#105665

Purpose

expresses thermostable variant of human carbonic anhydrase II with C-terminal 6x his tag

Depositor

Ryan Mehl

Publication

Mehl et al 2020

Insert

human carbonic anhydrase II thermostable variant (CA2 Human)

Tags

6x his tag

Expression

Bacterial

Promoter

pBad/arabinose

Available since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCXWB-EBNA1

Plasmid

#37624

Purpose

Non-replicating episomal expression of EBNA1, which enhances transfection efficiency and expression from episomal plasmids

Depositor

Shinya Yamanaka

Publication

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EBNA-1 (EBNA-1 human herpesvirus 4)

Expression

Mammalian

Promoter

CAG

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

73 Articles

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pCXWB-EBNA1

Plasmid

#37624

Purpose

Non-replicating episomal expression of EBNA1, which enhances transfection efficiency and expression from episomal plasmids

Depositor

Shinya Yamanaka

Publication

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EBNA-1 (EBNA-1 human herpesvirus 4)

Expression

Mammalian

Promoter

CAG

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

73 Articles

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