169,450 results
-
Plasmid#200985PurposeMammalian expression plasmid for soluble EphB3 fused to IgG1 FcDepositorInsertEphB3 (EPHB3 Human)
TagsFc region of human IgG1ExpressionMammalianMutationSoluble ectodomain of EphB3PromoterCMVAvailable SinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV500 A-SREBP1
Plasmid#33357DepositorInsertSREBP1 (Srebf1 Cricetulus griseus (hamster))
TagsFLAGExpressionMammalianMutationDominant Negative (see notes)PromoterCMVAvailable SinceDec. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry (PV2670) (AAV9)
Viral Prep#100061-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry (PV2670) (#100061). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry (PV2670) plasmid DNA. EFIa-driven, Cre-dependent, C1V1 (t/t) fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Cre-dependent)Available SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hDlx-GiDREADD-dTomato-Fishell-5 (AAV1)
Viral Prep#83896-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-hDlx-GiDREADD-dTomato-Fishell-5 (#83896). In addition to the viral particles, you will also receive purified pAAV-hDlx-GiDREADD-dTomato-Fishell-5 plasmid DNA. hDlx-driven expression of Gi-DREADD and nuclear localized dTomato (physically separate). These AAV preparations are suitable purity for injection into animals.DepositorPromoterhDlxTagsdTomato (physically separate, not a fusion protein)Available SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQTEV- PRDX2
Plasmid#31338DepositorAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
TRIPZ-bioGEF-UBnc-PURO
Plasmid#208044PurposeEnables inducible expression of low-affinity Avitag-UBnc; to perform bioE3; nc = non-cleavable mutation; selection with puromycinDepositorInsertUBnc (UBC Human)
UseLentiviralTagsLow-affinity AvitagMutationL73P Mutation near C-terminus suppresses cleavage…PromotertetO/UBCAvailable SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2904
Plasmid#143388PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-TRIM28deltaLinker
Plasmid#124958PurposeMammalian expression of Human TRIM28 with deleted Linker region between CC and PHD domainsDepositorInserthTRIM28 (TRIM28 Human)
TagsHAExpressionMammalianMutationDeletion of linker region between CC domain and P…Available SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-YTHDC2
Plasmid#222558PurposePiggyBac transposon plasmid for doxycycline inducible expression of YTHDC2DepositorInsertYTHDC2 (YTHDC2 Human)
ExpressionMammalianAvailable SinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Coff/Fon-sRGECO (AAV8)
Viral Prep#137128-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-Ef1a-Coff/Fon-sRGECO (#137128). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Coff/Fon-sRGECO plasmid DNA. EF1a-driven, Flp-dependent expression of cytosolic sRGECO calcium sensor (inhibited in presence of Cre). These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aAvailable SinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.Flag.HERC2-HECT-C4762A.6xHis
Plasmid#39228DepositorInsertHERC2 HECT Domain (HERC2 Human)
Tags6xHis and FlagExpressionMammalianMutationC4762APromoterT7Available SinceJuly 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
evoBE4-NG
Plasmid#226852PurposeExpresses evoBE4max-NG base editor in mammalian cells with eGFP markerDepositorInsertevoBE4max-NG
ExpressionMammalianPromoterCMVAvailable SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCiGSΔΨ
Plasmid#108312PurposeFoamy virus helper plasmid expressing Gag, driven by a CMV promoter. Ampicillin resistant.DepositorInsertgag
ExpressionMammalianMutationpCiGSΔΨ is a newer version of the pCiGS Gag expre…PromoterCMVAvailable SinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pN3-3xFlag-Control
Plasmid#107717PurposeControl expression plasmid for 3xFLAG-tagged pN3-contructsDepositorTypeEmpty backboneTags3xFlagExpressionMammalianAvailable SinceApril 3, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40
Plasmid#133560PurposeBackbone vector for CRISPR-HDR insertion of SPARC-Variant constructs (or any module) near the attP40 locusDepositorTypeEmpty backboneUseCRISPRExpressionInsectAvailable SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
LV TRE-GFP-TMD-DLL1
Plasmid#194936PurposeLV construct encoding GFP-TMD-DLL1 ligand under dox-inducible TRE promoter and containing a PGK-rtTA-M2-IRES-Puro regulator/resistance marker cassette.DepositorInserteGFP-(PDGFR)TMD-rDLL1 ICD (WT)
UseLentiviralAvailable SinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCHW111040
Plasmid#222451PurposeLevel 1, Position 3 containing CBS4-CMV::RUBY::OCS.DepositorInsertCBS4-CMV1::RUBY::OCS
UseSynthetic BiologyExpressionPlantMutationnoAvailable SinceMay 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-DDB1 (1140H)
Plasmid#174849PurposeExpression of DDB1DepositorInsertDDB1 (DDB1 Human)
ExpressionMammalianAvailable SinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE-80L MBP-SspB Micro
Plasmid#60410PurposeE. coli expression of SspB MicroDepositorInsertSspB micro
TagsHis-MBP-TEVExpressionBacterialMutationR73Q, Y11K and A15EAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLX209-neo-WRN K577M
Plasmid#125790Purposeconstitutive, ectopic expression of a helicase-dead mutant of human WRN (K577M)DepositorAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Sox2
Plasmid#101761PurposeSox2 promoter reporterDepositorAvailable SinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-SADB19G
Plasmid#32633DepositorInsertSADB19G
ExpressionMammalianPromoterCMVAvailable SinceOct. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5_FRT_TO_TurboID-mCherry-Cry2
Plasmid#166504PurposeExpression of your protein of interest fused with TurboID and mcherry-CRY2 for optogenetic activation, Flp-In T-Rex system. Search for client proteins within light-induced biomolecular condensates.DepositorInsertNone
TagsTurboID and mCherry-CRY2ExpressionMammalianAvailable SinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
C321.∆A.opt
Bacterial Strain#87359PurposeThis is C321.∆A with additional mutations engineered by Kuznetsov et al. to improve doubling time.DepositorBacterial ResistanceGentamicinSpeciesSyntheticAvailable SinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC035 - dLwCas13a-msfGFP
Plasmid#91925PurposeExpresses catalytically inactive LwCas13a for mammalian RNA targetingDepositorInsertdLwCas13a
UseCRISPRTagsNLS-3xHA and NLS-msfGFPExpressionMammalianMutationR488A and R1060APromoterEF-1aAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
U6-5'+3' MS2-CircRNA
Plasmid#213752PurposeFor circular RNA-mediated prime editor using U6-5'+3' MS2-CircRNA in HEK293T cellsDepositorInsert5' ribozyme, 5' ligation sequences, 5' MS2, 3' MS2, 3' ligation sequences, 3' ribozyme
UseCRISPRExpressionMammalianPromoterU6Available SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX 4T-1 GST-Ago2 S387A
Plasmid#60656PurposeExpresses GST-Ago2 S387A mutant in mammalian cellsDepositorAvailable SinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
LEPG
Plasmid#111160PurposemiR-E (miR-30 variant)-based RNAi with GFP reporterDepositorInsertmiR-E (miR-30 variant)
UseRetroviralMutationWTAvailable SinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSin-EF2-LIN28-Pur
Plasmid#16580DepositorAvailable SinceJan. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-DAPK1
Plasmid#116727PurposeLentiviral expression of DAPK1DepositorAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only