177,450 results
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Plasmid#105499PurposeExpression of Drosophila ISWI C-terminally tagged with Combined Sce VMA intein and chitin binding domain (iCBD)DepositorInsertISWI-iCBD
Available SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-truncated
Plasmid#198286PurposeContains a tail fiber with a truncated binding domain (lacking aa403-476 in Pvc13); produces nontargeting PVCsDepositorInsertPVCpnf_pvc13-truncated
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-hISG15
Plasmid#12447DepositorAvailable SinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-hISG15
Plasmid#12447DepositorAvailable SinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisD-moxMaple3
Plasmid#120871PurposeBacterial expression of moxMaple3DepositorInsertmoxMaple3
TagsHisExpressionBacterialMutationfrom mMaple3, moxMaple3 was engineered by replaci…Available SinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJS169
Plasmid#48882PurposeNegative feedback plasmid from oscillatorDepositorInsertlacI with ssrA tag
UseSynthetic BiologyTagsssrA (TSAANDENYALAA)ExpressionBacterialPromoterPara/lacAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHR_AXP_hrGFP
Plasmid#84613PurposeHR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into AXP locusDepositorInserthrGFP
UseSynthetic BiologyExpressionYeastPromoterUAS1B8-TEF(136)Available SinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pc DNA FRT TO- APPSwed/Ind
Plasmid#114194PurposeCreation of a stable cell line expressing APP mutation (AD in vitro study)DepositorInsertAmyloid-Precursor-Protein (APP Human)
ExpressionMammalianMutationPopular mouse model, overexpress 2 mutations: Swe…PromoterCMVAvailable SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJS167
Plasmid#48881PurposePositive feedback plasmid from oscillatorDepositorInsertsaraC gene with ssrA tag
monomeric yeast-enhanced green fluorescent protein
UseSynthetic BiologyTagsssrA (TSAANDENYALAA)ExpressionBacterialPromoterPara/lacAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pICH41295
Plasmid#47997DepositorTypeEmpty backboneUseUnspecifiedAvailable SinceDec. 3, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223_NOTCH1_ICN
Plasmid#82087PurposeGateway Donor vector containing NOTCH1 , part of the Target Accelerator Plasmid Collection.DepositorInsertNOTCH1 (NOTCH1 Human)
UseGateway entry vectorMutationICN (AA1761-2556 present)PromoterNoneAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMET17p-CRU313
Plasmid#131163PurposeFor ScMET17 promoter regulated expression of Cub-R-URA3 fused bait proteins, centromeric ARS plasmid, HIS3 complements the histidine auxotrophy of S. cerevisiae his mutantDepositorTypeEmpty backboneTagsCRUExpressionYeastAvailable SinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tol2-ngn1-Trpa1b-2A-mCherry
Plasmid#106425PurposeFor expressing Trpa1b-2A-mCherry in zebrafish Rohon-Beard neurons.DepositorAvailable SinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
NbAlfa-GFP2
Plasmid#201485PurposeBRET2 detection of ALFA-tagged proteinDepositorInsertNbAlfa-GFP2
TagsGFP2ExpressionMammalianPromoterCMVAvailable SinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Puro-IKBalpha-mut (super repressor)
Plasmid#15291DepositorInsertinhibitor of Kappa B alpha (NFKBIA Human)
UseRetroviralExpressionMammalianMutationS32A/S36A: “super-repressor” allele (resistant to…Available SinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Puro-IKBalpha-mut (super repressor)
Plasmid#15291DepositorInsertinhibitor of Kappa B alpha (NFKBIA Human)
UseRetroviralExpressionMammalianMutationS32A/S36A: “super-repressor” allele (resistant to…Available SinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
GIPR-DuET
Plasmid#213252PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro Xlone-eGFP
Plasmid#136936Purposedonor plasmid for inducible transgene expression in human cellsDepositorInsertall-in-one tet-on system
UseSynthetic BiologyExpressionMammalianPromoterTRE3GSAvailable SinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEVA331Bb
Plasmid#78269PurposepSEVA331 with Biobrick multiple cloning siteDepositorTypeEmpty backboneAvailable SinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDB5318
Plasmid#204828PurposeAn integrating plasmid containing both the inducible enotetS promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.DepositorInsertstetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetS promoterAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAB1676 REPAIR.t1
Plasmid#176319PurposeCMV-HIVNES-GS-dCas13bt1- (GGS)2-huADAR2dd(E488Q) (REPAIR.t1 expression)DepositorInsertsUseCRISPRTagsHIV NESExpressionMammalianMutationE488Q, only the deaminase domain (aa 276-702 are …PromoterCMVAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX-Z4 (GB3375)
Plasmid#160648PurposepLX series: RK2-based T-DNA binary vector for plant transformation (alpha1)DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
ND4.2-Left TALE-G1397-N-DddA11-mCherry
Plasmid#179682PurposeExpression of base editor in mammalian cells and for cell sortingDepositorInsertCOX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (S1330I A1341V N1342S E1370K T1380I)_1xUGI_P2A_mCherry_ATP5B 3'UTR
ExpressionMammalianMutationS1330I A1341V N1342S E1370K T1380IAvailable SinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCROPseq-VEX-optimized-scaffold
Plasmid#203321PurposeModified CROPseq vector for efficient CRISPR screens in hematopoiesisDepositorInsertsgRNA
UseCRISPR and LentiviralExpressionMammalianAvailable SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHBS1501 [IBB-GFP-mCherry3E]-[BFP-TDP43 deltaRRM]
Plasmid#133325PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationDeletion of RRM in full-length TDP43Available SinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLexm
Plasmid#99844Purposemammalian expressionDepositorTypeEmpty backboneExpressionMammalianAvailable SinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
Recall-miniCMV-sfGFP
Plasmid#137995PurposeBackbone for generation of ClonMapper Recall PlasmidDepositorTypeEmpty backboneExpressionMammalianAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
loxP-G418-LoxP-TurboID-Rab11 HR
Plasmid#230026PurposeHomology repair plasmid for endogenous tagging of Rab11 at the N-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cellsDepositorAvailable SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMXS-mitoGshF-blasticidin
Plasmid#210314PurposeExpresses mitochondrially targeted, humanized Streptococcus thermophilus GshF in mammalian cellsDepositorInsertBifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)
UseRetroviralTagsFLAG peptide, Homo sapiens ACO2 mitochondrial tar…MutationCodon optimized for expression in human cellsAvailable SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
plenti.CamKII.(cyto).iATPSnFR2.A95K.miRFP670nano3
Plasmid#209727PurposeExpresses cytosolic medium affinity ratiometric ATP sensorDepositorInsert(cyto).iATPSnFR2.A95K.miRFP670nano3
UseLentiviralExpressionMammalianPromoterCamKIIAvailable SinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUGW-APPwt-T2A-mCherry
Plasmid#190803PurposeExpress wild-type APP from Ubiquitin C promoter, co-expressing mCherryDepositorAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
Flag-cIAP1/pRK5
Plasmid#27972DepositorAvailable SinceFeb. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
DD-DamV133A-tdTomato-LMNB1
Plasmid#159604PurposeShield-1 inducible transient mammalian expression of Dam-tdTomato-LMNB1 for DamID, containing the V133A mutant of Dam and a fused tdTomato to monitor fusion protein localization and expression levels.DepositorInsertDD-DamV133A-tdTomato-LMNB1
ExpressionMammalianMutationDam V133A mutationPromoterCMVAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pDB5050
Plasmid#171126PurposeA plasmid containing the codon-optimized OsTIR1, which is under the control of adh1 promoter. It served as one of the F-box protein expression plasmids in AID system.DepositorInsertOsTIR1
UseOtherMutationnon applicableAvailable SinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_CMV::hTfR-GFP
Plasmid#199714PurposeExpresses a chimera of human transferrin receptor (hTfR) and GFP in mammalian cells.DepositorInserthTfR-GFP
UseAAVExpressionMammalianPromoterCMVAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131-tRNA2.0
Plasmid#158393PurposeGolden Gate entry vector to express the 1st gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterWithout promoterAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+-mutTornado-split-nLuc
Plasmid#212707PurposeTornado split nLuc with a mutated 3' ribozyme so that circularization cannot occur.DepositorInsertmutTornado-CVB3-split-nLuc
MutationDeleted the partial 3'ribozyme sequence in T…Available SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
STING-V1
Plasmid#124262PurposeC-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartmentsDepositorAvailable SinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
p5155
Plasmid#175391PurposeLow Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts >15kb) into dual site mouse strains (Bxb-GT/GA)DepositorTypeEmpty backboneUseMouse Targeting; Cloning vector, to be used to de…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only