Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

163,682 results

Showing: 11761 - 11800 of 163682 results

  1. pLinker-6xHA-HXGPRT-LoxP

    Plasmid
    #86552
    Purpose
    The plasmid is used for endogenous tagging using CRISPR technology in Toxoplasma
    Depositor
    David Sibley
    Insert
    Linker-6xHA-HGPRT-LoxP
    Use
    Expression in toxoplasma gondii
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    86552-plasmid-map-sequence-id-190138

    Enlarge

  2. PB-sgRNA(MS2)

    Plasmid
    #102560
    Purpose
    Piggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.
    Depositor
    Ying Liu
    Article
    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    102560-plasmid-map-sequence-id-198595

    Enlarge

  4. TFORF0831

    Plasmid
    #142208
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZBTB33 (ZBTB33 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    142208-plasmid-map-sequence-id-397699

    Enlarge

  6. pSL1180-HR-PUbECFP

    Plasmid
    #47917
    Purpose
    Donor plasmid for homologous recombination that expresses ECFP consitutively using the Aedes aegypti polyubiquitin (PUb) promoter.
    Depositor
    Leslie Vosshall
    Insert
    ECFP
    Use
    Tags
    NLS
    Expression
    Insect
    Mutation
    Promoter
    Aedes aegypti polyubiquitin (PUb)
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only
    47917-plasmid-map-sequence-id-68604

    Enlarge

  8. pCAG2-EGFP-C-IFT144

    Plasmid
    #218740
    Purpose
    Expresses C-terminally EGFP-tagged IFT144 in mammalian cells
    Depositor
    Kazuhisa Nakayama
    Insert
    IFT144 (WDR19 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    218740-plasmid-map-sequence-id-434235

    Enlarge

  10. mCherry-SSTR3-N-17

    Plasmid
    #55135
    Purpose
    Localization: Cilium, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    SSTR3
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55135-plasmid-map-sequence-id-329523

    Enlarge

  14. pGEM4Z-T7-5'UTR-NanoLuc-3'UTR-A64

    Plasmid
    #203350
    Purpose
    Template for T7 in vitro transcription of NanoLuc mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail
    Depositor
    Molly Stevens, Christopher Grigsby
    Insert
    Nano Luciferase
    Use
    Template for t7-mediated in vitro transcription o…
    Tags
    Flanked by 5' and 3' UTRs from Xenopus …
    Expression
    Mutation
    Promoter
    T7
    Available since
    June 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    203350-plasmid-map-sequence-id-399148

    Enlarge

  16. PB-TA-ERP2

    Plasmid
    #80477
    Purpose
    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and puromycin resistance)
    Depositor
    Knut Woltjen
    Article
    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:
    Type
    Empty backbone
    Use
    Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80477-plasmid-map-sequence-id-179966

    Enlarge

  17. XEC

    Plasmid
    #233341
    Purpose
    expressing SARS-CoV-2 XEC spike protein for pseudovirus production
    Depositor
    David Nemazee
    Insert
    Spike (XEC) (S SARS-CoV-2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    T22N, F59S on the basis of KP.3
    Promoter
    CMV
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    233341-plasmid-map-sequence-id-472417

    Enlarge

  18. KP.3.1.1

    Plasmid
    #233342
    Purpose
    expressing SARS-CoV-2 KP.3.1.1 spike protein for pseudovirus production
    Depositor
    David Nemazee
    Insert
    Spike (KP.3.1.1) (S SARS-CoV-2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    S31del on the basis of KP.3
    Promoter
    CMV
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    233342-plasmid-map-sequence-id-472416

    Enlarge

  20. sAB-K29

    Plasmid
    #204735
    Purpose
    synthetic antigen-binding fragment that can specifically recognize K29-linked polyubiquitin
    Depositor
    Minglei Zhao
    Article
    Yu et al Nat Chem Biol. 2021 Aug;17(8):896-905. d
    Insert
    synthetic antigen-binding fragment that can specifically recognize K29-linked polyubiquitin
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    204735-plasmid-map-sequence-id-412652

    Enlarge

  27. AAV-hSyn-Ribo-jGCaMP8s (AAV1)

    Viral Prep
    #167572-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from AAV-hSyn-Ribo-jGCaMP8s (#167572). In addition to the viral particles, you will also receive purified AAV-hSyn-Ribo-jGCaMP8s plasmid DNA. Synapsin-driven neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Marianne Fyhn
    Article
    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1
    Promoter
    Syn
    Tags
    None
    Available since
    Dec. 18, 2024
    Availability
    Academic Institutions and Nonprofits only

  34. Rhesus AAVS1-CAG-copGFP

    Plasmid
    #84209
    Purpose
    Rhesus AAVS1 safe harbor gene targeting donor expressing CAG-driven copGFP
    Depositor
    Cynthia Dunbar
    Inserts
    Rhesus AAVS1 5’ homology arm
    Rhesus AAVS1 3’ homology arm
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    84209-plasmid-map-sequence-id-166261

    Enlarge

  37. pCMV-PE6f

    Plasmid
    #207856
    Purpose
    Mammalian expression of PE6f prime editor
    Depositor
    David Liu
    Article
    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.
    Insert
    PE6f
    Use
    Tags
    SV40 bpNLS and SV40 bpNLS, c-Myc NLS
    Expression
    Mammalian
    Mutation
    Cas9(H840A, E471K, H99R, I632V, H721Y, D645N, K91…
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    207856-plasmid-map-sequence-id-408168

    Enlarge

  38. pAAV_hSyn1-SIO-stGtACR2-FusionRed (AAV Retrograde)

    Viral Prep
    #105677-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV_hSyn1-SIO-stGtACR2-FusionRed (#105677). In addition to the viral particles, you will also receive purified pAAV_hSyn1-SIO-stGtACR2-FusionRed plasmid DNA. Synapsin-driven, Cre-dependent, soma-targeted anion-conducting channelrhodopsin fused to FusionRed for optogenetic inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ofer Yizhar
    Article
    Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1
    Promoter
    Syn
    Tags
    FusionRed (Cre-dependent)
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 11761 - 11800 of 163682 results