-
-
pBAD/HisD-rsTagRFP
Plasmid#31927DepositorInsertrsTagRFP
UseTagsHisExpressionBacterialMutationF84W/I125L/N148A/S165G/F181L/K189N/Y200F compared…PromoteraraBAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
CoV2-N-T205I
Plasmid#177954PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (T205I). Used for generating RNA packaging virus-like particles.DepositorInsertSARS-CoV-2 Nucleocapsid (T205I) (N )
UseTagsExpressionMammalianMutationT205IPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-Dual-iCas9-CMV
Plasmid#167500PurposeExpression of dual inducible Cas9 with CMV promoterDepositorInsertCas9
UseCRISPR, Lentiviral, and LuciferaseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Flag-TurboID-NES_pLX304
Plasmid#202008Purposeexpresses TurboID in the mammalian cytosol, lentiviral vectorDepositorInsertFlag-TurboID-NES
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGW-GFP-TurboID-GOI
Plasmid#209381PurposeGateway compatible plasmid for TurboID-based proximity labelling experiment in plantaDepositorTypeEmpty backboneUseTagsExpressionPlantMutationTurboID gene sequence C249APromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3-enCjCas9-BlastR
Plasmid#220978PurposeExpresses human codon-optimized enCjCas9DepositorInsertenCjCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-FlipGFP(Caspase-1 cleavage seq) T2A mCherry
Plasmid#124494PurposeExpresses FlipGFP (caspase-1 cleavage sequence) and T2A mCherryDepositorInsertFlipGFP(Caspase-1 cleavage seq) T2A mCherry
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBR322-PVCpnf18-21
Plasmid#198273PurposeContains only regulatory genes; for generating empty PVCsDepositorInsertPVCpnf18-21
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUG-Pten
Plasmid#140732PurposeLentiviral vector to overexpress wild-type human PTEN as a GFP-PTEN fusion proteinDepositorInsertGFP-Pten (PTEN Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterhUbiCAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBPHLWL-VP16AD
Plasmid#26259DepositorInsertVP16AD-ZIP+
UseDrosophila transgenesisTagsExpressionBacterial and InsectMutationPromoterAvailable sinceFeb. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-PCP-GFPnls
Plasmid#121938PurposeCRISPR-Sirius plasmidDepositorInsertPCP-GFPnls
UseLentiviralTagssfGFPExpressionMammalianMutationPromoterEFSAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6 asyn WT
Plasmid#107425PurposeMammalian expression of human alpha synucleinDepositorInsertalpha-synuclein (SNCA Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCap-SG5-Apr
Plasmid#227579PurposeCapture plasmid with apramycin resistance and pSG5 repliconDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-27:SLC25A17-mEGFP
Plasmid#101784PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human SLC25A17DepositorInsertSLC25A17 Homology Arms with linker-mEGFP (SLC25A17 Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceOct. 31, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
BII-CMV-AfmW3A
Plasmid#133402PurposePiggybac vector for constitutive expression of Afamin + Wnt3a for production of conditioned media in HEK293T cellsDepositorUseTagsExpressionMammalianMutationN/APromoterCMVAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBabe-HAZ
Plasmid#17383DepositorTypeEmpty backboneUseRetroviralTagsHAExpressionMammalianMutationPromoterAvailable sinceMarch 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV/D374Y-hPCSK9
Plasmid#58379PurposeExpresses GoF mutant human PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosisDepositorInsertproprotein convertase subtilisin/kexin type 9 (PCSK9 Human)
UseAAVTagsExpressionMammalianMutationD374YPromoterHCRApoE/hAATAvailable sinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
Ub-GyrA
Plasmid#128737PurposeExpresses Ub fused to GyrA intein at the C-terminalDepositorInsertUBC
UseTagsMxe GyrAExpressionBacterialMutationPromoterT7Available sinceSept. 18, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
mEmerald-Alpha-Actinin2-N-18
Plasmid#53988DepositorInsertAlpha-Actinin2
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223_SPOP_p.K101I
Plasmid#81641PurposeGateway Donor vector containing SPOP, part of the Target Accelerator Plasmid Collection.DepositorInsertSPOP (SPOP Human)
UseGateway entry vectorTagsExpressionMutationK101IPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_SPOP_p.W131C
Plasmid#81643PurposeGateway Donor vector containing SPOP, part of the Target Accelerator Plasmid Collection.DepositorInsertSPOP (SPOP Human)
UseGateway entry vectorTagsExpressionMutationW131CPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHyVec2
Plasmid#34790DepositorTypeEmpty backboneUseHydra expressionTagsExpressionMutationPromoterHydra vulgaris actin promoterAvailable sinceJan. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
v3em-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP
Plasmid#207863PurposeAAV genome encoding C-terminal PE6d and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNADepositorInsertNpuC-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP
UseAAVTagsExpressionMutationSee ManuscriptPromoterCbhAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLCKO2
Plasmid#125518PurposeLentiviral backbone for cloning and expressing U6 driven sgRNAs with BsmBI cloning sites and puromycin selection.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
R6K_tac_GFPmut3_ChInt
Plasmid#187381Purposechromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible GFPmut3DepositorInsertGFPmut3
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR-GFP-MDC1 (770-7)
Plasmid#26284DepositorInsertMediator of DNA damage checkpoint 1 (MDC1 Human)
UseEntry vectorTagsGFPExpressionMutationPromoterAvailable sinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
MSCV-miRE_shBRD9_561-SV40-GFP
Plasmid#75139PurposeRetroviral expression plasmid encoding shBRD9_561 (targeting human BRD)DepositorInsertshBRD9_561
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
pLX317-SNAI1
Plasmid#115446PurposeConstitutive lentiviral expressionDepositorInsertSNAI1 (SNAI1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
3xnls-mScarlet-I
Plasmid#98816PurposeIn vivo visualization of the nucleus (can be used for colocalization studies)DepositorArticleInsert3xNLS
UseTagsmScarlet-IExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
beta2-spectrin-HA
Plasmid#31070DepositorInsertbeta2-spectrin (SPTBN1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDV05.2_pEF1a-iRFP720_secreted
Plasmid#200409PurposeExpresses a secreted version of iRFP720 constitutively in mammalian cellsDepositorInsertiRFP720 (secreted)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
EF1α-Lox2272-LoxP-MARKS·mTurquoise2-NLS·turboRFP-Lox2272-LoxP
Plasmid#198753PurposeCre reporter, which switches from membraneous mTurquoise to nuclear RFP upon Cre recombinase activityDepositorInsertEF1a promotor-Lox2272-LoxP-MARKS-mTurquoise2-NLS.turboRFP-Lox2272-LOXP
UseLentiviralTagsExpressionBacterial and MammalianMutationSee depositor comments belowPromoterEF1aAvailable sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2556
Plasmid#144042PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertADNP2 (ADNP2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
LRP8-bio-His
Plasmid#51879PurposeExpresses full-length Low-density lipoprotein receptor-related protein 8 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertLRP8 (LRP8 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2-N1
Plasmid#54525PurposeLocalization: N1 Cloning Vector, Excitation: 507 / 572, Emission: 516 / 580DepositorTypeEmpty backboneUseTagsmEos3.2ExpressionMammalianMutationPromoterCMVAvailable sinceJune 12, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
Bassik lab Mouse CRISPR-Cas9 Deletion Library - Drug targets, kinases, phosphatases
Pooled Library#1000000122PurposeCRISPR gRNA mouse knockout pooled library - Drug targets, kinases, phosphatasesDepositorAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only
