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Showing: 8881 - 8910 of 282703 results

  3. pAAV-hDlx-Flex-GFP-Fishell_6 (AAV1)

    Viral Prep
    #83895-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-hDlx-Flex-GFP-Fishell_6 (#83895). In addition to the viral particles, you will also receive purified pAAV-hDlx-Flex-GFP-Fishell_6 plasmid DNA. hDlx-driven, Cre-dependent expression of GFP. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Article
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Promoter
    hDlx
    Tags
    GFP (Cre-dependent)
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only

  5. pRDA_867

    Plasmid
    #231109
    Purpose
    A>G base editor
    Depositor
    David Root, John Doench
    Insert
    ABE8e-nCas9-SpG-HA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    D10A
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    231109-plasmid-map-sequence-id-458425

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  6. pME-Cas9-T2A-GFP

    Plasmid
    #63155
    Purpose
    Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.
    Depositor
    Leonard Zon
    Article
    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15
    Insert
    Cas9-T2A-GFP
    Use
    CRISPR; Tol2 middle entry vector for gateway
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    63155-plasmid-map-sequence-id-220487

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  8. p189

    Plasmid
    #65207
    Purpose
    Mutant EGFP expression plasmid for the creation of a heteroduplex DNA repair assay plasmid with pGEM5Z(+)-EGFP (p111). *Please see notes in comments section.
    Depositor
    LuZhe Sun
    Article
    Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. d
    Insert
    EGFP
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Premature stop codon added to pGEM5Z(+)-EGFP (TGG…
    Promoter
    CMV
    Available since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. pRRLsin-SV40 T antigen-IRES-mCherry

    Plasmid
    #58993
    Purpose
    lentiviral expression of SV40 large T antigen and mCherry red fluorescent reporter
    Depositor
    Snorri Thorgeirsson
    Article
    Holczbauer et al Gastroenterology. 2013 Jul;145(1):221-31
    Inserts
    CMV enhancer-chicken beta actin promoter
    SV40 large T antigen
    IRES-mCherry red fluorescent protein
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    EcoRV site in MCS converted to BamHI site
    Promoter
    CMV enhancer-chicken beta actin
    Available since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    58993-plasmid-map-sequence-id-457463

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  10. pGEM5Z(+)-EGFP

    Plasmid
    #65206
    Purpose
    EGFP expression plasmid (also called p111) for the creation of a heteroduplex DNA repair assay plasmid with p189. *Please see notes in comments section.
    Depositor
    LuZhe Sun
    Article
    Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. d
    Insert
    EGFP
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Restriction enzyme recognition sites added either…
    Promoter
    CMV
    Available since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  17. pEGFP rbFOX1

    Plasmid
    #63085
    Purpose
    Mammalian expression of EGFP tagged FOX1
    Depositor
    Nicolas Charlet-Berguerand
    Insert
    RBFOX1 (RBFOX1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63085-plasmid-map-sequence-id-104484

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  19. pAAV.CMV.PI.EGFP.WPRE.bGH (AAV2)

    Viral Prep
    #105530-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA. CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    CMV
    Tags
    EGFP
    Available since
    June 29, 2018
    Availability
    Academic Institutions and Nonprofits only

  23. pAAV.hSynapsin.SF-iGluSnFR.A184S

    Plasmid
    #106174
    Purpose
    Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Has Service
    AAV1 and AAV9
    Insert
    SF-iGluSnFR.A184S
    Use
    AAV
    Tags
    Expression
    Mutation
    GltI: A184S
    Promoter
    hSynapsin
    Available since
    Feb. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    106174-plasmid-map-sequence-id-204113

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  30. pSecUAG-Evol2

    Plasmid
    #163148
    Purpose
    Expresses machinery necessary for selenocysteine incorporation at UAG codons
    Depositor
    Dieter Söll
    Article
    Mukai et al Angew Chem Int Ed Engl. 2018 Jun 11;57(2
    Inserts
    allo-tRNA UTu2D
    thioredoxin
    Selenophosphate synthase
    Selenocysteine synthase
    Cysteine desulfurase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Cysteine 32 switched to Selenocysteine (UAG), Cys…
    Promoter
    EM7, araBAD, native As SelD promoter, and native …
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    163148-plasmid-map-sequence-id-331409

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Showing: 8881 - 8910 of 282703 results