167,318 results

1. pcDNA-R-iLACCO1.2

   Plasmid

   #208027

   Purpose

   Biosensor for intracellular L-lactate

   Depositor

   Robert Campbell

   Article

   Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

   Insert

   R-iLACCO1.2
   

   Expression

   Mammalian

   Available Since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-PGK-Hygro-PIP-FUCCI

   Plasmid

   #138714

   Purpose

   Lentiviral expression of the cell cycle marker PIP-FUCCI

   Depositor

   Jean Cook

   Article

   Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

   Insert

   PIP-FUCCI
   

   Use

   Lentiviral

   Tags

   mCherry and mVenus

   Expression

   Mammalian

   Promoter

   hPGK

   Available Since

   May 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-FLAG-CRBN-pGK-HYG

   Plasmid

   #107380

   Purpose

   Mammalian expression of FLAG-CRBN

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   cereblon (CRBN Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-hATG7wt

   Plasmid

   #87867

   Purpose

   Express a wild type human Atg7/Apg7 in mammalian cells

   Depositor

   Isei Tanida, Eiki Kominami

   Article

   Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

   Insert

   ATG7 (ATG7 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-pCMV-dSaCas9-VP64-pU6-sgRNA

   Plasmid

   #158990

   Purpose

   Vector G encodes pAAV-pCMV-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in mammalian cells

   Depositor

   Chung Tin

   Article

   Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

   Insert

   dSaCas9-VP64
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   pCMV

   Available Since

   Aug. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. CAG-BFP-sesRNA-GFP-WPRE-pA

   Plasmid

   #192063

   Purpose

   Expression of BFP, sesRNA in mammalian cells, with GFP as efRNA.

   Depositor

   Josh Huang

   Article

   Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

   Insert

   sesRNA for tdTom
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGloSensor -42F cGMP Vector

   Plasmid

   #236806

   Purpose

   Express -42F cGMP in biosensor assay

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   -42F
   

   Use

   Luciferase

   Mutation

   None

   Promoter

   CMV

   Available Since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. opto-E-cad GFP

   Plasmid

   #203327

   Purpose

   Expresses E-cadherin with a LOV2 insert after T133 for blue light inhibition in mammalian cells

   Depositor

   Seraphine Wegner

   Article

   Nzigou Mombo et al Nat Commun. 2023 Oct 9;14(1):6292. doi:

   Insert

   Photoswitchable - E-cadherin with GFP tag (CDH1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Addition of Aslov2 domain at T133 of human E-cadh…

   Available Since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-M13-TevC-IRES-SP6-Cre

   Plasmid

   #171620

   Purpose

   M13-TevC with Cre

   Depositor

   Hyungbae Kwon

   Article

   Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

   Insert

   M13-TevC-IRES-SP6-Cre
   

   Use

   AAV and Synthetic Biology

   Tags

   6x His

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Flag-SIRT1 H363Y

    Plasmid

    #1792

    Depositor

    Michael Greenberg

    Article

    Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

    Insert

    SIRT1 (SIRT1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    H363Y, deacetylase domain mutation

    Available Since

    May 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSFDuet-1_VNp15-mNeongreen

    Plasmid

    #182389

    Purpose

    Bacterial expression of Vesicle Nucleating peptide15-mNeongreen fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp15-mNeongreen-His
    

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL3-Basic-IRES

    Plasmid

    #64784

    Purpose

    Luciferase reporter vector

    Depositor

    Joshua Mendell

    Article

    Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

    Type

    Empty backbone

    Use

    Luciferase

    Available Since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1-TetON-Puro-HIF1a 3809

    Plasmid

    #118705

    Purpose

    Lentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)

    Depositor

    William Kaelin

    Article

    Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

    Insert

    shRNA against human HIF1a
    

    Use

    Lentiviral

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. mMaroon-C1

    Plasmid

    #54835

    Purpose

    Localization: C1 Cloning Vector, Excitation: 610, Emission: 652

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    mMaroon

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pMyNT

    Plasmid

    #42191

    Depositor

    Annabel Parret, Matthias Wilmanns

    Article

    Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

    Type

    Empty backbone

    Tags

    His6

    Expression

    Bacterial

    Promoter

    ColE1

    Available Since

    Aug. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. peGFP C3-SPG20 8xV

    Plasmid

    #218579

    Purpose

    Expresses GFP-tagged human SPG20 bulky hydrophobic amino acid mutant in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

    Insert

    SPG20 (SPART Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    changed Tryptophan 419, Leucine 438, Isoleucine 4…

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3puro-mGFP-ROCK2

    Plasmid

    #225472

    Purpose

    Express mEGFP-fusion protein of ROCK2

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    ROCK2 (ROCK2 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG_smFP V5

    Plasmid

    #59758

    Purpose

    encodes multiple copies of an epitope tag V5

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Insert

    smFP _V5
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHM840

    Plasmid

    #20701

    Depositor

    Thomas Stamminger

    Article

    Sorg et al Biotechniques. 1999 May . 26(5):858-62.

    Insert

    GFP-NLS-lacZ
    

    Expression

    Mammalian

    Available Since

    April 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-15b-UbcH5b/2419

    Plasmid

    #11475

    Depositor

    Allan Weissman

    Article

    Jensen et al J Biol Chem. 1995 Dec 22. 270(51):30408-

    Insert

    UbcH5b (UBE2D2 Human)
    

    Expression

    Bacterial

    Mutation

    n/a

    Available Since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

21. pINDUCER21-RORA

    Plasmid

    #51303

    Purpose

    Expression of RORA after doxycycline administration

    Depositor

    George Daley

    Article

    Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

    Insert

    RAR-related orphan receptor A (RORA Human)
    

    Use

    Lentiviral

    Tags

    n/a

    Mutation

    full length ORF

    Available Since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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22. pET-Expotin 1

    Plasmid

    #42047

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    Exportin-1 (XPO1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Feb. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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23. Mouse GeCKO Lentiviral sgRNA Library (LentiGuide-Puro)

    Pooled Library

    #1000000053

    Purpose

    CRISPR gRNA knockout pooled library for targeting the mouse genome

    Depositor

    Feng Zhang

    Available Since

    July 23, 2014

    Availability

    Academic Institutions and Nonprofits only

24. pAAV-hSyn1-mNeonGreen

    Plasmid

    #99135

    Purpose

    An AAV genome that ubiquitously expresses the fluorescent protein mNeonGreen from the hSyn1 promoter

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    mNeonGreen
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn1

    Available Since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. pET51b-His-TEV-HaloTag7

    Plasmid

    #167266

    Purpose

    Overexpression of HaloTag7 in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    HaloTag7
    

    Tags

    His x10

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFRT-TODestFLAGHAhFMRPiso1

    Plasmid

    #48690

    Purpose

    Expresses hFMRP iso 1

    Depositor

    Thomas Tuschl

    Article

    Ascano M et al Nature. 2012 Dec 20;492(7429):382-6. doi

    Insert

    FlagHA FMR1 Iso 1 (FMR1 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Promoter

    CMV, 2x Tet

    Available Since

    May 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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27. Human SHIP1 (clone hp51CN)

    Plasmid

    #123347

    Purpose

    Expression of human SHIP1

    Depositor

    Christophe Erneux

    Article

    Drayer et al Biochem Biophys Res Commun. 1996 Aug 5;2

    Insert

    INPP5D (INPP5D Human)
    

    Expression

    Mammalian

    Available Since

    April 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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28. pGL4.23-GW

    Plasmid

    #60323

    Purpose

    This is a modified version of the pGL4.23 vector from Promega, containing a Gateway cassette upstream of the minimal promoter. This vector can be used for for Gateway cloning of candidate enhancer elements. As negative control, use pGL4.23 without the gateway cassette (available from Promega).

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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29. BL21ΔBCF

    Bacterial Strain

    #102269

    Purpose

    lamB, ompC, ompF triple knockout strain for overproduction of outer membrane proteins

    Depositor

    Jack Leo

    Article

    Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

    Bacterial Resistance

    None

    Available Since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

30. pET28a hMDH1v3 (+TEV)

    Plasmid

    #204229

    Purpose

    Bacterial expression of human cytosolic malate dehydrogenase isoform 1 transcript variant 3 (hMDH1v3); Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    hMDH1v3 (MDH1 Human)
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Inserted a G after ATG to maintain kozak site

    Promoter

    Lac

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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