We narrowed to 4,524 results for: GCA

1. NFATc3-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

   Plasmid

   #124288

   Purpose

   sgRNA against human NFATc3

   Depositor

   Katalin Josvay, Csaba Vizler

   Insert

   NFATc3 nuclear factor of activated T cells (NFATC3 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

2. pSLQ1653_sgSOD1

   Plasmid

   #63711

   Purpose

   Express sgSOD1 in mammalian cells of human.

   Depositor

   Stanley Qi

   Article

   Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.

   Insert

   sgSOD1, CAG promoter, mCherry, puro
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUCSh8-2300

   Plasmid

   #170986

   Purpose

   Encodes sgRNA target 2300 and Hygromycin resistance transposon.

   Depositor

   Christopher Reisch

   Article

   Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

   Insert

   Sh-CAST sgRNA 2300
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCRISPR-Cas9-ScaligD

   Plasmid

   #125688

   Purpose

   Gene knockout/in for actinomycetes

   Depositor

   Tilmann Weber

   Article

   Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

   Insert

   streptomyces codon optimized spCas9, sgRNA casette, and ScaligD
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   ermE*/tipA

   Available since

   Nov. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUCSh5vio-2300

   Plasmid

   #170987

   Purpose

   Encodes sgRNA target 2300 and Gentamicin resistance - violacein pathway transposon.

   Depositor

   Christopher Reisch

   Article

   Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

   Insert

   Sh-CAST sgRNA 2300 violacein
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   placI

   Available since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPEgRNA

   Plasmid

   #172716

   Purpose

   Delivery of guide RNA for prime editing

   Depositor

   Tilmann Weber

   Article

   Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

   Insert

   gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pnsgRNA

   Plasmid

   #172717

   Purpose

   Delivery of guide RNA for prime editing

   Depositor

   Tilmann Weber

   Article

   Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

   Insert

   J23119-gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LRT2B-GO2

   Plasmid

   #136909

   Purpose

   Lentivirus expressing GO2 sgRNA

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   sgGO2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   hU6

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pColE1_sgRNA_NT_AmpR

   Plasmid

   #119144

   Purpose

   Encodes non-targeting sgRNA

   Depositor

   Vincent Noireaux

   Article

   Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

   Insert

   non-targeting sgRNA
   

   Use

   Crispr

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pENTR APP shRNA 1

    Plasmid

    #30134

    Depositor

    Dennis Selkoe, Tracy Young-Pearse

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    APP shRNA 1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pCZGY2162

    Plasmid

    #110136

    Purpose

    Epidermal Mitochondrial-tagged Florescent Calcium Sensor

    Depositor

    Andrew Chisholm

    Article

    Xu et al Dev Cell. 2014 Oct 13;31(1):48-60. doi:

    Insert

    mito::GCaMP3
    

    Use

    Tags

    mito::GCaMP3

    Expression

    Worm

    Mutation

    Promoter

    Pcol-19

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAH233

    Plasmid

    #121144

    Purpose

    Expression of gRNA with BbsI placeholder for target cloning

    Depositor

    Katsunori Tanaka

    Article

    Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

    Insert

    gRNA cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    S.pombe rrk1

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. MYC sgRNA5

    Plasmid

    #100557

    Purpose

    Expresses MYC sgRNA5. Target sequence GAGAGGCAGAGGGAGCGAGC

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

    Insert

    MYC sgRNA5
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MYC sgRNA4

    Plasmid

    #100556

    Purpose

    Expresses MYC sgRNA4. Target sequence: GTAATTCCAGCGAGAGGCAG

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

    Insert

    MYC sgRNA4
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pQdCas12a.luxR(mut)-sggfp(A)

    Plasmid

    #236039

    Purpose

    The plasmid pQdCas12a.luxR(mut)-sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.

    Depositor

    Radhakrishnan Mahadevan

    Insert

    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    quorum sensing promoter

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUCSh2-2300

    Plasmid

    #170984

    Purpose

    Encodes sgRNA target 2300 and Kanamycin resistance transposon.

    Depositor

    Christopher Reisch

    Article

    Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

    Insert

    Sh-CAST sgRNA 2300
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUCSh3-2300

    Plasmid

    #170985

    Purpose

    Encodes sgRNA target 2300 and Tetracycline resistance transposon.

    Depositor

    Christopher Reisch

    Article

    Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

    Insert

    Sh-CAST sgRNA 2300
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pZac2.1 gfaABC1D-tdTomato

    Plasmid

    #44332

    Depositor

    Baljit Khakh

    Article

    Shigetomi et al J Gen Physiol. 2013 Apr 15.

    Has Service

    AAV5

    Insert

    tdTomato
    

    Use

    AAV; Adeno-associated virus ()

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSWAP_Entry_T2

    Plasmid

    #184864

    Purpose

    pSwap plasmid part containing the T2 sgRNA module for the Swap and Drop recombination system.

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pSWAP_Entry_T1

    Plasmid

    #184863

    Purpose

    pSwap plasmid part containing the T1 sgRNA module for the Swap and Drop recombination system.

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pJYS2_crtYf

    Plasmid

    #85544

    Purpose

    Constitutive transcription of crRNA of crtYf, Spectinomycin resistance

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Insert

    crRNA of crtYf
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLH-nmsgRNA1.1

    Plasmid

    #64115

    Purpose

    Vector for expression of Nm sgRNA1.1 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Nm sgRNA1.1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PLKO.1-SMG6-CDS-1

    Plasmid

    #136046

    Purpose

    SMG6 shRNA (Targeting CDS #1) inserted into the PLKO.1 plasmid (AACTTGTAAGTAACCTGCAGC)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    SMG6 (SMG6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCRISPR-dCas9

    Plasmid

    #125687

    Purpose

    Gene knockdown for actinomycetes

    Depositor

    Tilmann Weber

    Article

    Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

    Insert

    streptomyces codon optimized spdCas9, sgRNA casette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    ermE*/tipA

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLH-stsgRNA3.1

    Plasmid

    #64118

    Purpose

    Vector for expression of St1 sgRNA3.1 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 sgRNA3.1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pG4

    Plasmid

    #162605

    Purpose

    Edit Ade2 gene in yeast

    Depositor

    Lars Steinmetz

    Article

    Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

    Insert

    Tef1-Cas9 with RPL25 Intron
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Level 1 P4 TaU6 guide acceptor

    Plasmid

    #165600

    Purpose

    GoldenGate (MoClo) Level 1 Position 4 TaU6 guide acceptor plasmid

    Depositor

    John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood

    Article

    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

    Insert

    TaU6 promoter::LacZ::sgRNA scaffold
    

    Use

    CRISPR and Synthetic Biology; Sgrna acceptor with…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Level 1 P3 TaU6 guide acceptor

    Plasmid

    #165599

    Purpose

    GoldenGate (MoClo) Level 1 Position 3 TaU6 guide acceptor plasmid

    Depositor

    John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood

    Article

    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

    Insert

    TaU6 promoter::LacZ::sgRNA scaffold
    

    Use

    CRISPR and Synthetic Biology; Sgrna acceptor with…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJYS2cm_crtYf

    Plasmid

    #85543

    Purpose

    Constitutive transcription of crRNA of crtYf, Chloramphenicol resistance

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Insert

    crRNA of crtYf
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSK-Sasg

    Plasmid

    #214350

    Purpose

    Expresses cloning backbone for SlugCas9 sgRNA

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugCas9 tracrRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDropVn

    Plasmid

    #184873

    Purpose

    Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for Vibrio natriegens genome editing protocol.

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. ATG16L1 sgRNA

    Plasmid

    #207563

    Purpose

    pX330 expressing Cas9 and a sgRNA targeting the ATG16L1 locus

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    GCAGCAAGTGACATGTCGTC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. MK1274

    Plasmid

    #71431

    Purpose

    This E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 300 and a TetR translation rate of 35149.

    Depositor

    Howard Salis

    Article

    Kushwaha et al Nat Commun. 2015 Jul 17;6:7832. doi: 10.

    Insert

    Mixed Feedback Loop version of the UBER system
    

    Use

    Tags

    Expression

    Mutation

    T7 RBS:AACCGAGCCCAATATAGGACTCAGGGTGCCAAAAAA and T…

    Promoter

    Available since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. 53BP1 N-terminal sgRNA

    Plasmid

    #207091

    Purpose

    pX330 based plasmid for expression of Cas9 and the GGGGAGCAGATGGACCCTAC sgRNA to target the 53BP1 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    GGGGAGCAGATGGACCCTAC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pX-EGFP-g1

    Plasmid

    #107273

    Purpose

    eGFP sgRNA-1 and Cas9 expression vector (aka. pX-ps1)

    Depositor

    Knut Woltjen

    Article

    Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

    Insert

    GFP sgRNA-1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLentiX2-PURO-shGFP

    Plasmid

    #61230

    Purpose

    Lentivirus expressing shRNA to GFP

    Depositor

    Reuben Shaw

    Article

    Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    H1/TO

    Available since

    March 20, 2015

    Availability

    Academic Institutions and Nonprofits only

37. pBa.VAMP2-GFP-SBP

    Plasmid

    #180249

    Purpose

    Mammalian expression of VAMP2

    Depositor

    Marvin Bentley

    Article

    Nabb et al Mol Biol Cell. 2021 Nov 3:mbcE21080425.

    Insert

    VAMP2 (Vamp2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Chicken Beta actin

    Available since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pALPS puro miR30-IRF1

    Plasmid

    #117156

    Purpose

    Target site TTGCTCTTAGCATCTCGGCTG

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    shRNA targeting miR30
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pUC19-U6-AksgRNA

    Plasmid

    #121955

    Purpose

    Mammalian expression, Genome editing, gRNA scaffold

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AkCas12b single chimeric gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pUC19-U6-AasgRNA

    Plasmid

    #121954

    Purpose

    Mammalian expression, Genome editing, gRNA scaffold

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AaCas12b single chimeric gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge