We narrowed to 14,684 results for: nts

1. MSP2135

   Plasmid

   #72248

   Purpose

   Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A, and D1135E

   Promoter

   CMV

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pECE M2-SNX17 WT

   Plasmid

   #69811

   Purpose

   Expresses M2-SNX17 in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   Sorting nexin-17 (SNX17 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   SV40 early promoter

   Available Since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAF0362 Ef1a-Cp36_attP-Puro-p2a-mCherry donor

   Plasmid

   #193469

   Purpose

   Cp36 Donor with promoter for mcherry and puro expression

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Cp36_attP
   

   Expression

   Mammalian

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX6P1-human full-length LIC1

   Plasmid

   #74597

   Purpose

   used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag

   Depositor

   Ron Vale

   Article

   Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

   Insert

   full length human dynein light intermediate chain 1 (DYNC1LI1 Human)
   

   Tags

   N-terminal GST and Strep II

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJP-CoV2leader-nLuc-TSS

   Plasmid

   #175430

   Purpose

   Expresses nLuc with 5' leader of CoV2 in mammalian cells.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   nLUC plus 5' CoV2 leader
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. p663-UBC-miniTurbo-V5-NRF3

   Plasmid

   #207815

   Purpose

   N-terminal tagged NRF3 for miniTurbo proximity experiments

   Depositor

   Ben Major

   Article

   LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

   Insert

   miniTurbo-V5-NRF3 (NFE2L3 Human)
   

   Use

   Lentiviral

   Tags

   miniTurbo-V5

   Promoter

   UBC

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. p667-UBC-NRF1-V5-miniTurbo

   Plasmid

   #207810

   Purpose

   C-terminal tagged NRF1 for miniTurbo proximity experiments

   Depositor

   Ben Major

   Article

   LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

   Insert

   NRF1-V5-miniTurbo (NFE2L1 Human)
   

   Use

   Lentiviral

   Tags

   V5-miniTurbo

   Promoter

   UBC

   Available Since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. p663-UBC-miniTurbo-V5-NRF1

   Plasmid

   #207809

   Purpose

   N-terminal tagged NRF1 for miniTurbo proximity experiments

   Depositor

   Ben Major

   Article

   LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

   Insert

   miniTurbo-V5-NRF1 (NFE2L1 Human)
   

   Use

   Lentiviral

   Tags

   miniTurbo-V5

   Promoter

   UBC

   Available Since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. p667-UBC-NRF3-V5-miniTurbo

   Plasmid

   #207816

   Purpose

   C-terminal tagged NRF3 for miniTurbo proximity experiments

   Depositor

   Ben Major

   Article

   LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

   Insert

   NRF3-V5-miniTurbo (NFE2L3 Human)
   

   Use

   Lentiviral

   Tags

   V5-miniTurbo

   Promoter

   UBC

   Available Since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJT396 PB ITR and Cp36 attD pEF Puro-2a-tagBFP2 donor

    Plasmid

    #184952

    Purpose

    Donor for both PiggyBac and Cp36 Recombinase

    Depositor

    Michael Bassik, Ami Bhatt, Patrick Hsu, Lacra Bintu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    PuroR, P2A, mTagBFP2
    

    Expression

    Mammalian

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. VVT1

    Plasmid

    #65779

    Purpose

    The Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    SaCas9 gRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIS1-Vim/Actb5UTR-renilla

    Plasmid

    #38238

    Depositor

    David Sabatini

    Article

    Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

    Insert

    Vim promoter with Actb 5UTR/Renilla (Vim, Actb Mouse)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Actb promoter (1000 nt upstream) and 5' UTR …

    Promoter

    Endogenous Vim

    Available Since

    Nov. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pGEX C_K nsp1 CoV2

    Plasmid

    #175516

    Purpose

    Expresses C_K for bioconjugation on nsp1

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    C_K nsp1 CoV2
    

    Tags

    GST tag

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-mCherry-p2A-LIC1 Y101A, Y103A

    Plasmid

    #74605

    Purpose

    expresses LIC1 in mammalian cells; HA-tagged; point mutants that abrogate binding to dynein

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    full length human dynein light intermediate chain 1 Y101A, Y103A (DYNC1LI1 Human)
    

    Use

    Lentiviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Y101A, Y103A

    Promoter

    SFFV

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX nsp1 R99A CoV2

    Plasmid

    #175515

    Purpose

    For protein expression of R99A nsp1 CoV2

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    R99A nsp1 CoV2
    

    Tags

    GST tag

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX nsp1 K164A/H165A CoV2

    Plasmid

    #175514

    Purpose

    For protein expression of K164A/H165A nsp1 CoV2

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    K164A/H165A nsp1 CoV2
    

    Tags

    GST tag

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX nsp1 R124A/K125A CoV2

    Plasmid

    #175513

    Purpose

    For protein expression of R124A/K125A nsp1 CoV2

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    R124A/K125A nsp1 CoV2
    

    Tags

    GST tag

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX C_K nsp1 K164A/H165A CoV2

    Plasmid

    #175517

    Purpose

    Expresses C_K for bioconjugation on nsp1

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    C_K nsp1 CoV2
    

    Tags

    GST tag

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBS NL4-3 envFS eGFP 5'CMV Δtat

    Plasmid

    #101347

    Purpose

    tat ATG->ACG (silent in vpr reading frame), nt 78 mutated T->G to change Tyr to stop codon, nt 116 mutated T->C to disrupt Met, 5'LTR replaced with CMV-R-U5 from pALPS for tat-independent transcription in HEK293E cells.

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3
    

    Use

    Lentiviral

    Mutation

    delta Tat

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pECE M2-SNX17 S437A

    Plasmid

    #69812

    Purpose

    Expresses M2-SNX17 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    Sorting nexin-17 (SNX17 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    SV40 early promoter

    Available Since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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