We narrowed to 14,165 results for: nts

1. 2Bc-T MBP_BoMoC(ed)_6xH

   Plasmid

   #186461

   Purpose

   Recombinant B. mori truncated R2 RT expression

   Depositor

   Kathleen Collins

   Article

   Upton et al Proc Natl Acad Sci U S A. 2021 Oct 19;11

   Insert

   B. mori truncated R2 RT
   

   Use

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pColdI-DDX3_helicase_core

   Plasmid

   #233618

   Purpose

   Expression of His-tagged helicase core region of DDX3X in E.coli

   Depositor

   Shintaro Iwasaki

   Article

   Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

   Insert

   DDX3X helicase core WT (DDX3X Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. psiCHECK2-Eef2TOP-RLCP-HCVIRES-FL

   Plasmid

   #233628

   Purpose

   Expression of bicistronic reporter with TOP motif

   Depositor

   Shintaro Iwasaki

   Article

   Shichino et al Nat Struct Mol Biol. 2024 Oct;31(10):155

   Insert

   Promoter and 5'UTR of Eef2 gene and HCV IRES (Eef2 Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNTI194

   Plasmid

   #84266

   Purpose

   Expresses Avi-tagged RPL1 and humanized birA

   Depositor

   Nicholas Ingolia

   Article

   Ingolia et al Cell Rep. 2014 Sep 11;8(5):1365-79. doi:

   Inserts

   RPL1 (RPL10A Human, (*see below))
   humanized birA
   

   Use

   Tags

   Avi tag, Flag, Gly/Ser linker, and TEV site

   Expression

   Mammalian

   Mutation

   R33A, A34E (*see below)

   Promoter

   CMV and P2A peptide

   Available since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJT305 Pa01 Lenti pEF-attB LSR GFP

   Plasmid

   #184948

   Purpose

   Lentiviral delivery of recombinase landing pad

   Depositor

   Lacra Bintu, Michael Bassik, Patrick Hsu, Ami Bhatt

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Pa01 Lenti pEF1a-attB LSR GFP
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJW1659

   Plasmid

   #154314

   Purpose

   NT Donor-cassette for SapTrap

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa linker::AID*::3xFLAG for NT slot
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA 3.2/V5-DEST RBM14-V5

   Plasmid

   #69823

   Purpose

   Expresses RBM14-V5 in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   RNA-binding protein 14 (RBM14 Human)
   

   Use

   Retroviral

   Tags

   v5

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   April 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA4 CoV-2 nsp1 3xFLAG

   Plasmid

   #176057

   Purpose

   Expressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG tag fusion to nsp1's C-terminus.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV-2 nsp1 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. LiPOP2

   Plasmid

   #168266

   Purpose

   A single-component optogenetic tool for photoswitchable pyroptosis

   Depositor

   Yubin Zhou

   Article

   He et al Adv Sci (Weinh). 2021 May 6;8(13):210042

   Insert

   mGSDMD(1-276) (Gsdmd Mouse)
   

   Use

   Tags

   cpLOV2, mCherry

   Expression

   Mammalian

   Mutation

   R138A, K146A, R152A, R154A

   Promoter

   CMV

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. psiCHECK2-Eef2TOPm-RLCP-HCVIRES-FL

    Plasmid

    #233629

    Purpose

    Expression of bicistronic reporter with mutated TOP motif

    Depositor

    Shintaro Iwasaki

    Article

    Shichino et al Nat Struct Mol Biol. 2024 Oct;31(10):155

    Insert

    Promoter and 5'UTR of Eef2 gene with mutation in TOP motif and HCV IRES (Eef2 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    AGAAGG in TOP motif

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJT301 BxBi Lenti pEF-attB LSR GFP

    Plasmid

    #184944

    Purpose

    Lentiviral delivery of recombinase landing pad

    Depositor

    Lacra Bintu, Michael Bassik, Patrick Hsu, Ami Bhatt

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    BxBi Lenti pEF1a-attB LSR GFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. cpLID-micro

    Plasmid

    #168995

    Purpose

    circularly permuted LOV2 based optical dimerization system (ssrA-cpLOV2 and sspB)

    Depositor

    Yubin Zhou

    Article

    He et al Nat Chem Biol. 2021 Aug;17(8):915-923. d

    Insert

    mCh-sspB-T2A-cpLID-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-AuroraB kd K106R

    Plasmid

    #108493

    Purpose

    expression of EGFP-AuroraB kinase dead (K106R)

    Depositor

    Susanne Lens

    Article

    Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

    Insert

    AuroraB (AURKB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Kinase death K106A

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pECE HA AMPKa1 WT

    Plasmid

    #69504

    Purpose

    Expresses AMPK alpha1 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    SV40 early promoter

    Available since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSP712

    Plasmid

    #65768

    Purpose

    Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Bacterial

    Mutation

    D10A and H840A mutations in Cas9

    Promoter

    T7 (x2)

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA 3.2/V5-DEST SNAP29-V5

    Plasmid

    #69821

    Purpose

    Expresses SNAP29-V5 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    Synaptosomal-associated protein 29 (SNAP29 Human)
    

    Use

    Retroviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    April 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE-NRF2-E79Q

    Plasmid

    #207828

    Purpose

    FLAG-tagged NRF2-E79Q driven by CMV promoter

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    NRF2-E79Q (NFE2L2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    E79Q

    Promoter

    CMV

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. CaV1.3e[8a,11,31b,Δ32,42a]

    Plasmid

    #49333

    Purpose

    Repaired Addgene #26576 to agree with the rat ref sequence. Repairs are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].

    Depositor

    Diane Lipscombe

    Article

    Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

    Insert

    CACNA1D (Cacna1d Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains exons 8a, 11, 31b and 42a.Deletion of ex…

    Promoter

    CMV

    Available since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJT306 Kp03 Lenti pEF-attB LSR GFP

    Plasmid

    #184949

    Purpose

    Lentiviral delivery of recombinase landing pad

    Depositor

    Lacra Bintu, Michael Bassik, Patrick Hsu, Ami Bhatt

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Kp03 Lenti pEF1a-attB LSR GFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pColdI-SBP-eIF4A1

    Plasmid

    #233621

    Purpose

    Expression of SBP-tagged eIF4A1 in E.coli

    Depositor

    Shintaro Iwasaki

    Article

    Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

    Insert

    SBP-eIF4A1 (EIF4A1 Human)
    

    Use

    Tags

    His6-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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