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We narrowed to 16,879 results for: Cas9

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Showing: 6101 - 6120 of 16879 results

  1. pCRISPRa_VPR_yl

    Plasmid
    #107677
    Purpose
    CRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing dCas9-VPR and AvrII site for sgRNA insertion
    Depositor
    Ian Wheeldon
    Publication
    Schwartz et al Biotechnol J. 2018 May 5:e1700584. doi:
    Inserts
    Codon optimized dCas9-VPR
    sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available since
    May 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    107677-plasmid-map-sequence-id-209648

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  2. pCMV-Target-ACEmax

    Plasmid
    #139105
    Purpose
    Mammalian Target-ACEmax expression vector (pSI-625)
    Depositor
    Nozomu Yachie
    Publication
    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    139105-plasmid-map-sequence-id-273275

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  3. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-Phi29 (JO582)

    Plasmid
    #208954
    Purpose
    A variant CE1 construct with Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-Phi29(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A), Phi29(-exo;D169A)
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208954-plasmid-map-sequence-id-415743

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  4. pCMV-SaPE2-P2A-BSD

    Plasmid
    #174818
    Purpose
    Mammalian expression of SaCas9 prime editor 2 with P2A-BSD marker
    Depositor
    David Liu
    Publication
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SaPE2-P2A-BSD
    Tags
    SV40 bpNLS
    Expression
    Mammalian
    Mutation
    Detailed in manuscript
    Promoter
    CMV
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174818-plasmid-map-sequence-id-343609

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  5. pLV_hPGK-2xMeCP2NLS-dCas9-HA-2xSV40NLS-KOX1-NpNLS-P2A/T2A-mTagBFP2-2xMycNLS-WPRE

    Plasmid
    #254799
    Purpose
    Constituitve expression of dCas9-KOX1 with optimized nuclear localization in iPSC-derived neurons via lenti delivery
    Depositor
    Ophir Shalem
    Publication
    Cajka et al bioRxiv [Preprint]. 2024 Oct 19:2024.10.
    Insert
    2xMeCP2NLS-dCas9-HA-2xSV40NLS-KOX1-NpNLS-P2A/T2A-mTagBFP2-2xMycNLS
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    catalytically dead spCas9
    Promoter
    hPGK
    Available since
    Aug. 31, 2026
    Availability
    Academic Institutions and Nonprofits only
    254799-plasmid-map-sequence-id-520123

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  10. px330_PTEN exon 5 gRNA

    Plasmid
    #68348
    Purpose
    px330 with gRNA towards PTEN exon 5. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PTEN exon 5 gRNA
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68348-plasmid-map-sequence-id-119620

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  11. pdCASclos

    Plasmid
    #73640
    Purpose
    Transcriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824
    Depositor
    Sheng Yang
    Publication
    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9
    sgRNA to spo0A
    Use
    E.coli-clostridium shuttle vector
    Mutation
    D10A, H840A
    Promoter
    Pj23119 and ptb
    Available since
    June 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73640-plasmid-map-sequence-id-148604

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  15. pMTL3

    Plasmid
    #127966
    Purpose
    constitutive expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus
    Depositor
    Martin Kampmann
    Publication
    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AAVS1-CAG-KRAB-dCas9-BFP-KRAB
    Use
    TALEN
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Nov. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    127966-plasmid-map-sequence-id-249160

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  17. pCRISPReporter-mCherry

    Plasmid
    #65008
    Purpose
    E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with mCherry reporter.
    Depositor
    Mattheos Koffas
    Publication
    Cress et al ACS Synth Biol. 2015 Mar 30.
    Insert
    mCherry
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    User-defined
    Available since
    May 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    65008-plasmid-map-sequence-id-109895

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  18. pMTL5

    Plasmid
    #127967
    Purpose
    DHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus
    Depositor
    Martin Kampmann
    Publication
    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB
    Use
    TALEN
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Nov. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    127967-plasmid-map-sequence-id-249184

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Showing: 6101 - 6120 of 16879 results