We narrowed to 7,375 results for: ef1a

1. pAAV-Ef1a-Coff/Fon-GCaMP6F

   Plasmid

   #137124

   Purpose

   Intersectional viral expression of GCaMP6F in cells expressing Flp AND NOT Cre

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-GCaMP6F
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   Removed RSET tag

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Ef1a-DIO-mCherry-WPRE-pA

   Plasmid

   #37083

   Purpose

   Cre-dependent expression of mCherry

   Depositor

   Bernardo Sabatini

   Article

   Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

   Insert

   mCherry
   

   Use

   AAV and Cre/Lox; Cre-on

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   July 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Ef1a-Coff/Fon-mCherry

   Plasmid

   #137134

   Purpose

   Intersectional viral expression of mCherry in cells expressing Flp AND NOT Cre

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-mCherry
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   n/a

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCF525-mTagBFP2_EF1a-Hygro-P2A-mTagBFP2_lenti

   Plasmid

   #138315

   Purpose

   Lenti expression of mTgBFP2

   Depositor

   Jennifer Doudna

   Article

   Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

   Insert

   mTAGBFP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. lenti-EF1a-dCas9-VP64-Puro

   Plasmid

   #99371

   Purpose

   3rd generation lenti vector encoding dCas9-VP64 with 2A puromycin resistance marker (EF1a-dCas9-VP64-T2A-Puro-WPRE)

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Insert

   dCas9-VP64-T2A-Puro
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-FRT-EF1a-TVA-2A-N2cG

   Plasmid

   #172361

   Purpose

   Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from Flp-expressing neurons

   Depositor

   Yoav Ben-Simon, Peter Jonas

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   TVA + B19-N2cG
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EF1a-DIO-hM4D(Gi)-mCherry

   Plasmid

   #50461

   Purpose

   Double floxed Gi-coupled hM4D DREADD fused with mCherry under the control of EF1a promoter

   Depositor

   Bryan Roth

   Insert

   hM4D(Gi)-mCherry (CHRM4 Human)
   

   Use

   AAV

   Tags

   HA and mCherry

   Expression

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   EF1a

   Available Since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-U6-gRNA-EF1a-EGFP-CAAX

   Plasmid

   #167936

   Purpose

   gRNA entry vector

   Depositor

   Emma Rawlins

   Article

   Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

   Insert

   U6-gRNA backbone
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EF1a-DIO-ASAP3Kv-WPRE

   Plasmid

   #132330

   Purpose

   expresses ASAP3Kv(soma-located version of ASAP3, ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vector

   Depositor

   Michael Lin

   Article

   Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

   Insert

   ASAP3KV
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Dec. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. lenti EF1a-FLAG-dCas9-VPR

    Plasmid

    #114195

    Purpose

    Lentivirus compatible dCas9-VPR construct driven by the EF1a promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    dCas9-VPR
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-EF1a-GalT-IRES-Puromycin

    Plasmid

    #134862

    Purpose

    Expresses EGFP-tagged Golgi marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    beta 1,4-galactosyltransferase (B4GALT1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 83-398

    Promoter

    EF1a

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-EF1a-CCO-IRES-Puromycin

    Plasmid

    #134861

    Purpose

    Expresses EGFP-tagged inner mitochondrial membrane marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    cytochrome c oxidase subunit VIII (COX8A Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Ef1a-ConVERGD-eGFP-WPRE

    Plasmid

    #218745

    Purpose

    Provides AND intersectional (Cre+Flp) expression of eGFP

    Depositor

    Lindsay Schwarz

    Article

    Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-EF1a-DIO-hM3D(Gq)-mCherry

    Plasmid

    #50460

    Purpose

    Double floxed Gq-coupled hM3D DREADD fused with mCherry under the control of EF1a promoter

    Depositor

    Bryan Roth

    Insert

    hM3D(Gq)-mCherry (CHRM3 Human)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    EF1a

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLEX-EF1a ANXA11-mEmerald D40G

    Plasmid

    #164211

    Purpose

    pLEX lentivirus backbone expresses mEmerald tagged ANXA11 D40G mutant under EF1a promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mutation

    D40G

    Promoter

    EF1a

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLEX-EF1a ANXA11-mEmerald R346C

    Plasmid

    #164213

    Purpose

    pLEX lentivirus backbone expresses mEmerald tagged ANXA11 R346C mutant under EF1a promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mutation

    R346C

    Promoter

    EF1a

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLEX-EF1a ANXA11-mEmerald R235Q

    Plasmid

    #164212

    Purpose

    pLEX lentivirus backbone expresses mEmerald tagged ANXA11 R235Q mutant under EF1a promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mutation

    R235Q

    Promoter

    EF1a

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-Ef1a-DO_DIO-TdTomato_EGFP-WPRE-pA

    Plasmid

    #37120

    Purpose

    DO/DIO Cre-Switch. Expresses tdTomato in Cre negative cells, expresses EGFP in Cre positive cells

    Depositor

    Bernardo Sabatini

    Article

    Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

    Insert

    TdTomato-EGFP
    

    Use

    AAV and Cre/Lox; Cre-switch

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    July 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV EF1a DIO iChloC 2A dsRed

    Plasmid

    #70762

    Purpose

    An improved chloride-conducting channelrhodopsin for light-induced inhibition of neuronal activity in vivo. Cre inducible expression (double floxed inversed ORF)

    Depositor

    Thomas Oertner

    Article

    Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.103

    Inserts

    Channelrhodopsin-2
    red fluorescent protein
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    E83Q,E90R,E101S,D156N,T159C

    Promoter

    EF1a

    Available Since

    Jan. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-EF1a-mCherry-IRES-Dre

    Plasmid

    #55633

    Purpose

    Expresses Dre in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Inserts

    mCherry
    Dre
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a and Ef1a/IRES

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSIN4-EF1a-GATA1-IRES-Puro

    Plasmid

    #61062

    Purpose

    lentiviral vector for GATA1

    Depositor

    Igor Slukvin

    Article

    Elcheva et al Nat Commun. 2014 Jul 14;5:4372. doi: 10.

    Insert

    GATA1 (GATA1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    EF1alpha

    Available Since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-Ef1a-Con/Fon-oScarlet

    Plasmid

    #137136

    Purpose

    Intersectional viral expression of oScarlet in cells expressing both Cre AND Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-oScarlet
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    E95D

    Promoter

    EF1a

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHAGE2-EF1aL-Cre-IRES-NeoR-W

    Plasmid

    #126700

    Purpose

    lentiviral or plasmid based co-expression of Cre recombinase and a neomycin resistance cassette

    Depositor

    Darrell Kotton

    Article

    Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

    Insert

    Cre-IRES
    

    Use

    Cre/Lox and Lentiviral

    Tags

    SV40 NLS

    Expression

    Mutation

    Promoter

    Ef1a

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Lenti-EF1alpha-VP64-dCas9-p300_Blast

    Plasmid

    #192654

    Purpose

    3rd generation lenti vector encoding VP64-dCas9-p300 with 2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    VP64-dCas9-p300
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A; H840A in Cas9

    Promoter

    EF1a

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Lenti-EF1alpha-VP64-dCas9-VP64_Blast

    Plasmid

    #192650

    Purpose

    3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    VP64-dCas9-VP64
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1a

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-EF1a-DIO-JEDI-1P-Kv-WPRE

    Plasmid

    #202608

    Purpose

    Double floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector expressed under the mammalian promoter (EF1a)

    Depositor

    Francois St-Pierre

    Article

    Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

    Insert

    JEDI-1P-Kv
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-Ef1a-Con/Fon-mCherry

    Plasmid

    #137132

    Purpose

    Intersectional viral expression of mCherry in cells expressing both Cre AND Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-mCherry
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    EF1a

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-EF1a-Con/Fon-GCaMP6M

    Plasmid

    #137119

    Purpose

    Intersectional viral expression of GCaMP6M in cells expressing both Cre AND Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-GCaMP6M
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    Removed RSET tag

    Promoter

    EF1a

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-Ef1a-fDIO-eGFP-WPRE

    Plasmid

    #203843

    Purpose

    Flp-dependent EGFP expression

    Depositor

    Ivo Spiegel

    Article

    Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pX458-Ef1a-Cas9-H2B-GFP

    Plasmid

    #159654

    Purpose

    Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B GFP nuclear reporter.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge