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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 1551 - 1600 of 11733 results

  2. K785R Smarca4-sfGFP

    Plasmid
    #107057
    Purpose
    Expression of dominant-negative K785R Smarca4 (Brg1) with C-terminal super-folder GFP fusion.
    Depositor
    Jerry Crabtree, Courtney Hodges
    Article
    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7
    Insert
    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    Use
    Lentiviral
    Tags
    Super-folder GFP (sfGFP)
    Expression
    Mammalian
    Mutation
    K785R
    Promoter
    CAG
    Available Since
    April 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    107057-plasmid-map-sequence-id-201959

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  7. pAM4891 (pCV0003)

    Plasmid
    #120080
    Purpose
    Broad host range plasmid. Bacteria plasmid vector to test various parts and devices. Expression of GFP and a Kanamycin/Neomycin resistance gene in various cyanobacteria.
    Depositor
    Susan Golden
    Article
    Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku
    Insert
    Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-RSF1010Y25F, CV-aph1, CV-PconII-LTRBS-GFPmut2
    Use
    Other
    Mutation
    mobA Y25F
    Available Since
    Feb. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120080-plasmid-map-sequence-id-234472

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  8. CIBN-GFP-TMD

    Plasmid
    #79571
    Purpose
    expression of CIBN domain fused to GFP tagged mouse Syntaxin1 TMD domain (transmembrane domain and polybasic region)
    Depositor
    Pietro De Camilli, Olof Idevall-Hagren
    Article
    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10
    Insert
    syntaxin 1A (Stx1a Mouse)
    Tags
    CIBN and EGFP
    Expression
    Mammalian
    Mutation
    aa 249_288 (C-terminal region (polybasic stretch …
    Promoter
    CMV
    Available Since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    79571-plasmid-map-sequence-id-456519

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  26. ACBE

    Plasmid
    #204607
    Purpose
    Mammalian expression of adenine-to-cytosine base editor ACBE
    Depositor
    Dali Li
    Article
    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103
    Insert
    bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
    Use
    CRISPR
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)
    Available Since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    204607-plasmid-map-sequence-id-404719

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  27. pDG330

    Plasmid
    #100898
    Purpose
    SpCas9 with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100898-plasmid-map-sequence-id-195326

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  31. pCDNA5_FRT_TO_TurboID-mCherry-Cry2

    Plasmid
    #166504
    Purpose
    Expression of your protein of interest fused with TurboID and mcherry-CRY2 for optogenetic activation, Flp-In T-Rex system. Search for client proteins within light-induced biomolecular condensates.
    Depositor
    Angelos Constantinou
    Article
    Frattini et al Mol Cell. 2021 Jan 16. pii: S1097-2765(2
    Insert
    None
    Tags
    TurboID and mCherry-CRY2
    Expression
    Mammalian
    Available Since
    March 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    166504-plasmid-map-sequence-id-328214

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  37. GFP-LiMETER-v2

    Plasmid
    #113934
    Purpose
    Also termed as OptoPBer; Express an STIM1-derived optical tether to label and interrogate ER-plasma membrane contact sites (MCS) with light; visualized by GFP
    Depositor
    Yubin Zhou
    Article
    He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi
    Insert
    LOV2 and Stim1 polybasic domain (PB)
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    113934-plasmid-map-sequence-id-225307

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  49. LAMP2-bio-His

    Plasmid
    #51861
    Purpose
    Expresses full-length Lysosome-associated membrane glycoprotein 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    LAMP2 (LAMP2 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51861-plasmid-map-sequence-id-79751

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Showing: 1551 - 1600 of 11733 results