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pAAV.CAG.(cyto).cpSFGFP.tdTomato

Plasmid

#248076

Purpose

cpSFGFP control with non-responsive mIRFP670nano3

Depositor

Tim Brown

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

cpSFGFP.mIRFP670nano3

Use

AAV

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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pHCMV-10A1

Plasmid

#15805

Depositor

Miguel Sena-Esteves

Article

Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

Insert

10A1 Murine leukemia virus envelope mRNA

Expression

Mammalian

Available Since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

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FRP793_insul-(lexA-box)4-PminCYC1-Citrine-TCYC1

Plasmid

#58434

Purpose

Induces Citrine expression from a promoter containing 4 lexA boxes in yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

CitrineA206K

Expression

Yeast

Promoter

insul-(lexA-box)4-PminCYC1

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Plastin-C-10

Plasmid

#55438

Purpose

Localization: Fimbrin/Cytoskeleton, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Plastin (PLS1 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCSCG-TM(DPP4)-Fc-hST3Gal4 WT

Plasmid

#239825

Purpose

Displays Fc-fusion hST3Gal4 WT on mammalian cell surface via transmembrane domain of DPP4

Depositor

Sriram Neelamegham

Article

Hombu et al Small. 2025 May 26:e2502318. doi: 10.100

Insert

TM(DPP4)-Fc-hST3Gal4 WT

Use

Lentiviral

Tags

human IgG1 Fc region

Expression

Mammalian

Mutation

amino acids 1-42 of hST3Gal4 were deleted

Promoter

CMV promoter

Available Since

Aug. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pRS313-SCT1-S288C

Plasmid

#226266

Purpose

Plasmid expressing the SCT1 allele from S288C, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SCT1 (SCT1 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-mCherry

Plasmid

#197420

Purpose

Cas9 from S. pyogenes with 2A-mCherry, and cloning backbone for sgRNA

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

Cas9

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pG1

Plasmid

#162602

Purpose

Edit Ade2 gene in yeast

Depositor

Lars Steinmetz

Article

Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

Insert

Tef1-Cas9 with RPL25 Intron

Expression

Yeast

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pET30-R5-GFP

Plasmid

#17838

Depositor

Jay Keasling

Article

Shaikh et al Anal Chem. 2008 Feb 1. 80(3):886-90.

Insert

pET30-GFP

Use

Synthetic Biology

Tags

His, R5, and S-tag

Expression

Bacterial

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only

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CMV-mito-CitronRH

Plasmid

#158547

Purpose

Mitochondria-targeted nonbinding variant of direct-response citrate sensor

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

CitronRH

Expression

Mammalian

Promoter

cmv

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX_ECFP

Plasmid

#247757

Purpose

Lentiviral expression of ECFP in mammalian cells

Depositor

Alan Ashworth

Article

Abrams et al Cell Chem Biol. 2025 Nov 10:S2451-9456(2

Insert

ECFP

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Eps15-pmCherryN1

Plasmid

#27696

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Eps15 (EPS15 Human)

Tags

mCherry

Expression

Mammalian

Available Since

May 5, 2011

Availability

Academic Institutions and Nonprofits only

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pBAD-Citron1

Plasmid

#134300

Purpose

Bacterial expression of direct-response citrate sensor

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citron1

Tags

6-His tag

Expression

Bacterial

Promoter

pBAD

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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pRBC-sfGFP wild type

Plasmid

#174075

Purpose

Plasmid with p15a origin of replication expressing codon optimized superfolder GFP with C-terminal His6 tag, under T7 promoter

Depositor

Ryan Mehl

Article

Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

Insert

Superfolder GFP

Tags

His-6

Expression

Bacterial

Promoter

T7

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-roxSTOProx-ZsGreen

Plasmid

#51274

Purpose

Dre-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by Dre-recognition sites (rox) followed by ZsGreen

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

roxSTOProx-ZsGreen

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti_hU6-peg(ATP1A1-T804N)_mU6-BsmBI_Puro

Plasmid

#234070

Purpose

For lentiviral expression of user-specified pegRNA in tandem with epegRNA (ATP1A1-T804N) for ouabain co-selection

Depositor

Greg Findlay

Article

Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pBbAW4k-loxP-TT-loxP-mRFP1

Plasmid

#134405

Purpose

Fluorescent reporter for Cre recombinase activity. Cre removes a transcription terminator, leading to RFP production.

Depositor

Mary Dunlop

Article

Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

Insert

W4-loxP-TT-loxP-mRFP1

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

W4 (modified T7A1)

Available Since

Jan. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAJM.011

Plasmid

#108529

Purpose

TetR + PTet*-YFP Reporter. aTc sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

TetR + PTet*-YFP

Expression

Bacterial

Available Since

June 29, 2018

Availability

Academic Institutions and Nonprofits only

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T777T

Plasmid

#113082

Purpose

Enhanced RNAi vektor for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites and a lacZ coding sequence.

Depositor

Tibor Vellai

Article

Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

Type

Empty backbone

Use

RNAi

Expression

Bacterial

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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IFI16-FL (pcDNA3-FLAG)

Plasmid

#35064

Depositor

Cheryl Arrowsmith

Article

Liao et al Structure. 2011 Mar 9;19(3):418-29.

Insert

IFI16 (IFI16 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 4, 2012

Availability

Academic Institutions and Nonprofits only