We narrowed to 14,321 results for: cas9

Showing: 6661 - 6680 of 14321 results

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gh19

Plasmid

#106703

Purpose

expression of gRNA targeting GALNT19

Depositor

Eric-Paul Bennett

Publication

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GALNT19

Expression

Mammalian

Available since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1446

Plasmid

#111129

Purpose

Expresses FANCF

Depositor

Jacob Corn

Publication

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCF (FANCF Human)

Use

Lentiviral

Tags

HA

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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SGL40C.EFS.E2Crimson

Plasmid

#100894

Purpose

Lentiviral gRNA delivery

Depositor

Dirk Heckl

Publication

Labuhn et al Nucleic Acids Res. 2018 Feb 16;46(3):137

Insert

E2Crimson

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAP1698-4

Plasmid

#105242

Purpose

mammalian eGFP

Depositor

Geraldine Seydoux

Publication

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

eGFP

Expression

Bacterial

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pYZ145

Plasmid

#98405

Purpose

Donor DNA plasmid to introduce leu1 deletion in S. pombe. Linearization with Not1 digestion.

Depositor

Jef Boeke

Publication

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Inserts

upstream homologous region to delete leu1 in genome by recombination (apc2 Fission Yeast)
downstream homologous region to delete leu1 in genome by recombination (top2 Fission Yeast)

Use

CRISPR and Synthetic Biology

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pYZ149

Plasmid

#98407

Purpose

Donor DNA plasmid to introduce his3 deletion in S. pombe. Linearization with Not1 digestion.

Depositor

Jef Boeke

Publication

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Inserts

upstream homologous region to delete his3 in genome by recombination (alg2 Fission Yeast)
downstream homologous region to delete his3 in genome by recombination (cog8 Fission Yeast)

Use

CRISPR and Synthetic Biology

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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sg2.02 MUC4.1

Plasmid

#101152

Purpose

gRNA with two MCP binding sites

Depositor

Mazhar Adli

Publication

Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

Insert

MUC4 (MUC4 Human)

Use

CRISPR and Lentiviral

Promoter

mU6

Available since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pEJS507-pCDest2-noAcr-mTagBFP2-IRES

Plasmid

#85748

Purpose

Expresses mTagBFP2 only; used as a control

Depositor

Erik Sontheimer

Publication

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

mTagBFP2

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pX459_HR_Prnp_3

Plasmid

#78623

Purpose

pX459 vector encoding SpCas9-2A-Puro and a chimeric guide RNA targeting Prnp coding sequence

Depositor

Walker Jackson

Publication

Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

Insert

HR_Prnp_3 (Prnp Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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sgRNA2_IL1RN

Plasmid

#64142

Purpose

Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Publication

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA2_IL1RN (IL1RN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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sgRNA4_IL1RN

Plasmid

#64152

Purpose

Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Publication

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA4_IL1RN (IL1RN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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AID-C12*-BE4max

Plasmid

#216729

Purpose

Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and nCas9 (Cas9 with D10A mutation) in mammalian cells.

Depositor

Rahul Kohli

Publication

Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

Insert

AID-C12*-BE4max (AICDA Human)

Expression

Mammalian

Promoter

CMV

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAGM65905

Plasmid

#153217

Purpose

Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformation

Depositor

Sylvestre Marillonnet

Publication

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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APq5210

Plasmid

#66086

Purpose

co-expression of Cas9 and a sgRNA targeting K08F4.2 in the middle of the ORF

Depositor

Geraldine Seydoux

Publication

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APa4-2

Use

CRISPR

Expression

Worm

Promoter

U6

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only
scaffold-linker-U6

Plasmid

#89639

Purpose

construction of paired sgRNAs driven by two U6 promoters. Vector itself only has one U6 promoter.

Depositor

Wensheng Wei

Publication

Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pJAT43

Plasmid

#204299

Purpose

For introducing heat shock inducble B3 recombinase. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthparts.

Depositor

David Stern

Publication

Stern et al bioRxiv 2023.06.17.545412

Insert

ie1-EGFP

Use

CRISPR

Promoter

ie1

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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vHB178

Plasmid

#193457

Purpose

3xHA endogenous tagging of pandoravirus/mollivirus genes

Depositor

Chantal Abergel

Publication

Bisio et al Nat Commun. 2023 Jan 26;14(1):428. doi:

Insert

3xHA

Use

Pandoravirus/mollivirus expression

Promoter

----

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pLPC-mCh-ACTB-P2A-EGFP

Plasmid

#165158

Purpose

Expresses an ACTB gene fragment with P2A, flanked by mCherry and EGFP, in Mammalian cells

Depositor

Taekjip Ha

Publication

Zou et al Mol Cell. 2021 Apr 1;81(7):1553-1565.e8.

Insert

mCherry-ACTB-P2A-EGFP (ACTB Human)

Use

Retroviral

Tags

EGFP and mCherry

Expression

Mammalian

Promoter

CMV

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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LBJJ432

Plasmid

#117508

Purpose

AtEC_enh. promoter Golden Gate Level 0

Depositor

Jonathan D Jones

Publication

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:GGAG:pEC_enh:AATG:BsaI

Expression

Plant

Promoter

EC1.2:EC1.1

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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LBJJ383

Plasmid

#117517

Purpose

PCR template for sgRNA classic with polyT as terminator

Depositor

Jonathan D Jones

Publication

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:ACTA:pU6-26:sgRNA:polyT:TTAC:BpiI

Use

CRISPR

Expression

Plant

Promoter

AtU6-26

Available since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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