161,377 results

1. pU6_N7-sgRNA2

   Plasmid

   #190664

   Purpose

   Expresses U6-driven N7 sgRNA2 (poly-T mutated out), BsmbI dropout cassette for cloning

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   N7 sgRNA2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSB1C3-Lux

   Plasmid

   #109383

   Purpose

   Arabinose inducible expression of the Vibrio Fischery Lux operon. Used to test burden response in E. coli

   Depositor

   Tom Ellis

   Article

   Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

   Insert

   pARABAD-LUX operon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC0078 RESCUE

   Plasmid

   #130661

   Purpose

   pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375S/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

   Insert

   dRanCas13b-ADAR2dd (RESCUE)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pME-QF2

   Plasmid

   #83307

   Purpose

   codes for QF2 (truncated QF) in a gateway middle entry backbone.

   Depositor

   Marnie Halpern

   Insert

   QF
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJFRC12-10XUAS-IVS-myr::GFP

   Plasmid

   #26222

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Aug 9. ():.

   Insert

   myr::GFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 19, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1_crGE

   Plasmid

   #172933

   Purpose

   Crowding FRET sensor with mCerulean3 and mCitrine for mammalian cell expression. Linker comprises 2 helices and 3 GSG domains.

   Depositor

   Arnold Boersma

   Article

   Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

   Insert

   CrowdingSensorGE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-CD74-ROS1 nonmutant

   Plasmid

   #183813

   Purpose

   Express CD74-ROS1 fusion (C6;R34) in mammalian cells

   Depositor

   Aaron Hata

   Article

   Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

   Inserts

   ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
   CD74 molecule (CD74 Human)
   

   Use

   Lentiviral

   Tags

   The fusion protein of CD74 exon 1-6 and ROS1 exon…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNestin-EGFP

   Plasmid

   #38777

   Depositor

   Wei Cui

   Article

   Noisa et al Stem Cell Rev. 2010 Sep;6(3):438-49.

   Insert

   nestin 2nd intron enhancer with TK mini promoter (NES Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. FUGW-ER-TurboID

   Plasmid

   #200965

   Purpose

   3rd gen lentiviral plasmid for expressing endoplasmic reticulum localized TurboID with EGFP

   Depositor

   Keriann Backus

   Article

   Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

   Insert

   ER-TurboID
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pF151 pcDNA3.1(+)SOD1WT

    Plasmid

    #26397

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA5/FRT_CMV_NES-Caprola_on-mEGFP

    Plasmid

    #194686

    Purpose

    CMV driven expression of the consitutively active calcium recorder positive control Caprola_on in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_on-mEGFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. STING-V2

    Plasmid

    #124264

    Purpose

    C-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments

    Depositor

    Eric Schirmer

    Insert

    TMEM173 (STING1 Human)
    

    Use

    Tags

    Venus2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCBFD2Bad

    Plasmid

    #53574

    Purpose

    Produces an active CBFD (carotenoid β-ring 4-dehydrogenase) enzyme in E. coli when expression is induced with arabinose

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

    Insert

    cbfd2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT2K-CAGGS-T2TP

    Plasmid

    #114725

    Purpose

    expression in mammalian cells

    Depositor

    Martine Roussel

    Article

    Kawauchi et al Oncogene. 2017 Sep 14;36(37):5231-5242.

    Insert

    Tol2 transposase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHR-TRE3G-dCas9-EGFP-HaloTag

    Plasmid

    #169451

    Purpose

    expression of dCas9-EGFP-HaloTag

    Depositor

    Stanley Qi

    Article

    Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

    Insert

    dCas9-EGFP-HaloTag
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-scFv-GCN4-sfGFP-GB1-NLS-iLID-dWPRE

    Plasmid

    #121966

    Purpose

    Expresses fusion of single chain variable fragment recognizing SunTag, fluorescent protein sfGFP, and iLID which upon light activation binds to sspB.

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    scFv-GCN4
    

    Use

    Lentiviral

    Tags

    superfold GFP-GB1-NLS-iLID

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMThy1.1_hDDX5_FL

    Plasmid

    #88873

    Purpose

    constitutive expression of DDX5 in mammalian cells

    Depositor

    Dan Littman

    Article

    Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

    Insert

    DDX5 (DDX5 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB_EF1a-DjCas13d-msfGFP-Blast

    Plasmid

    #226008

    Purpose

    DjCas13d protein for RNA targeting in piggyBac backbone

    Depositor

    Silvana Konermann

    Article

    Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

    Insert

    DjCas13d RNA-targeting nuclease
    

    Use

    CRISPR

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST14-DogCatcher

    Plasmid

    #171772

    Purpose

    Expresses DogCatcher protein in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    DogCatcher
    

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. mPlum-pBAD

    Plasmid

    #54564

    Purpose

    Localization: Protein Expression Vector , Excitation: 590, Emission: 649

    Depositor

    Roger Tsien, Michael Davidson

    Article

    Wang et al Proc Natl Acad Sci U S A. 2004 Nov 30;10

    Type

    Empty backbone

    Use

    Tags

    6xHis and mPlum

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28a-MH6-Bsu

    Plasmid

    #163911

    Purpose

    Expresses Bsu large fragment for affinity purification

    Depositor

    Paul Freemont

    Article

    Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

    Insert

    Bsu polymerase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-FLEX-SaCas9-U6-sgSlc18a2

    Plasmid

    #124849

    Purpose

    Mutagenesis of Slc18a2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc18a2 (Slc18a2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEGFP Sec22b

    Plasmid

    #101918

    Purpose

    Expresses Sec22b in mammalian cells

    Depositor

    Thierry Galli

    Article

    Petkovic et al Nat Cell Biol. 2014 May;16(5):434-44. do

    Insert

    Sec22b (Sec22b Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCDNA5/FRT_CMV_NES-Caprola_09-mEGFP

    Plasmid

    #194685

    Purpose

    CMV driven expression of the calcium recorder Caprola_09 in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_09-mEGFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-U6-racRNA-A

    Plasmid

    #200824

    Purpose

    AAV transfer plasmid encoding a circularized RNA barcode A under U6 promoter and a subcellular localization protein binder under hSyn promoter

    Depositor

    Xiao Wang

    Article

    Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

    Inserts

    U6-racRNA
    V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
    

    Use

    AAV

    Tags

    C-Far, Flag, and V5

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLenti PGK Puro DEST (w529-2)

    Plasmid

    #19068

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Puro

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. lentiGuide-Hygro-dTomato

    Plasmid

    #99376

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A dTomato from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Inserts

    S. pyogenes sgRNA cassette
    Hygro-P2A-dTomato
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a and hU6

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. RCASBP-AP (A) (CC#37)

    Plasmid

    #15160

    Depositor

    Connie Cepko

    Article

    Fekete et al Mol Cell Biol. 1993 Apr . 13(4):2604-13.

    Insert

    PLAP (ALPP Human)
    

    Use

    Retroviral; Avian expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET-21a-PE2-His

    Plasmid

    #201973

    Purpose

    Express His-tagged PE2 in bacteria

    Depositor

    Scot Wolfe

    Article

    Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

    Insert

    PE2-HIS
    

    Use

    Tags

    His and c-myc NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAHYB-GFP

    Plasmid

    #85074

    Purpose

    Expression of GFP in Pichia pastoris

    Depositor

    Sheng Yang

    Article

    Yang et al Yeast. 2014 Apr;31(4):115-25.

    Insert

    GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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